BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,795,380	A→T	52.8%	V483E (GTA→GAA)	thrS ←	threonyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,795,380	0	A	T	52.8%	57.7 / inf	250	V483E (GTA→GAA)	thrS	threonyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base A (60/58); new base T (58/74); total (118/132)
Fisher's exact test for biased strand distribution p-value = 3.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

AACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATCCC  >  CP009273/1795234‑1795527

aaCCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGctgtact                                                                                                                                                   >  2:2576432/1‑149 (MQ=255)

            ctTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGc                                                                                                                                       <  2:3497933/149‑1 (MQ=255)

            ctTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGGTCATTGTCTTCGCCTACATCAGAAGCGCTCAGACGAGACGGCACAGAGAAGTCCAGCTGTACTGTACCGCACTGc                                                                                                                                       >  2:4456623/1‑149 (MQ=255)

                 gTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGc                                                                                                                                  <  1:1150965/149‑1 (MQ=255)

                 gTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGc                                                                                                                                  <  1:4270167/149‑1 (MQ=255)

                     ggATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGa                                                                                                                              >  1:574480/1‑149 (MQ=255)

                     ggATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGa                                                                                                                              >  1:2004891/1‑149 (MQ=255)

                      gATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGAt                                                                                                                             <  1:3630879/149‑1 (MQ=255)

                      gATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGAt                                                                                                                             <  2:4052026/149‑1 (MQ=255)

                       aTACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATc                                                                                                                            <  1:3942674/149‑1 (MQ=255)

                        tACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCACAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCg                                                                                                                           >  2:4000513/1‑149 (MQ=255)

                         aCCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGa                                                                                                                          <  2:1169221/149‑1 (MQ=255)

                          ccGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAg                                                                                                                         >  2:3657478/1‑149 (MQ=255)

                           cGATGAAACGTTCCATCGACCCCAGAAGTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAgg                                                                                                                        <  1:4434566/149‑1 (MQ=255)

                            gATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGc                                                                                                                       >  1:1278558/1‑149 (MQ=255)

                               gAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGt                                                                                                                    <  1:4536347/149‑1 (MQ=255)

                                aaaCGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTc                                                                                                                   <  2:4978307/149‑1 (MQ=255)

                                 aaCGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCTAGGCAGTCa                                                                                                                  <  1:3697469/149‑1 (MQ=255)

                                 aaCGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCa                                                                                                                  <  2:1315496/149‑1 (MQ=255)

                                  aCGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCAt                                                                                                                 >  1:2970796/1‑149 (MQ=255)

                                    gTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATAc                                                                                                               >  2:905725/1‑149 (MQ=255)

                                    gTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATAc                                                                                                               <  1:2305774/149‑1 (MQ=255)

                                    gTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATAc                                                                                                               <  2:4274996/149‑1 (MQ=255)

                                     ttCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACa                                                                                                              >  2:3787263/1‑149 (MQ=255)

                                      tCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAg                                                                                                             >  2:2659449/1‑149 (MQ=255)

                                        cATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAggg                                                                                                           >  2:1438862/1‑149 (MQ=255)

                                         aTCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGt                                                                                                          <  1:867737/149‑1 (MQ=255)

                                         aTCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGt                                                                                                          >  2:1581523/1‑149 (MQ=255)

                                           cGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTaa                                                                                                        >  2:1495881/1‑149 (MQ=255)

                                           cGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTaa                                                                                                        >  2:1497295/1‑149 (MQ=255)

                                            gACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTaaa                                                                                                       <  1:2713455/149‑1 (MQ=255)

                                            gACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTaaa                                                                                                       >  1:3729067/1‑149 (MQ=255)

                                            gACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTaaa                                                                                                       >  2:668153/1‑149 (MQ=255)

                                             aCCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAAt                                                                                                      >  2:4145011/1‑149 (MQ=255)

                                              ccccAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAAtt                                                                                                     >  2:2115154/1‑149 (MQ=255)

                                                ccAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCa                                                                                                   >  1:2102121/1‑149 (MQ=255)

                                                 cAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCaa                                                                                                  <  1:4778104/149‑1 (MQ=255)

                                                    aaTTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAAttt                                                                                               <  1:451270/149‑1 (MQ=255)

                                                     aTTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAAtttt                                                                                              >  2:4465389/1‑149 (MQ=255)

                                                     aTTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAAtttt                                                                                              <  1:1672689/149‑1 (MQ=255)

                                                       tGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCg                                                                                            <  1:320897/149‑1 (MQ=255)

                                                       tGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCg                                                                                            <  1:1153769/149‑1 (MQ=255)

                                                         cgcgGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGa                                                                                          <  1:1483604/149‑1 (MQ=255)

                                                         cgcgGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGa                                                                                          <  1:2876787/149‑1 (MQ=255)

                                                                aaTCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGa                                                                                   >  2:4649021/1‑149 (MQ=255)

                                                                aaTCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGa                                                                                   <  2:5118095/149‑1 (MQ=255)

                                                                  tCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGaaa                                                                                 >  1:1592782/1‑149 (MQ=255)

                                                                    aTTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAgc                                                                               >  2:2805855/1‑149 (MQ=255)

                                                                    aTTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAgc                                                                               <  1:280769/149‑1 (MQ=255)

                                                                     ttACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAgcg                                                                              <  2:96971/149‑1 (MQ=255)

                                                                     ttACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAgcg                                                                              >  2:4525539/1‑149 (MQ=255)

                                                                       aCCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGcc                                                                            <  2:3152865/149‑1 (MQ=255)

                                                                       aCCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGcc                                                                            <  2:2400449/149‑1 (MQ=255)

                                                                        ccGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCt                                                                           <  2:5150230/149‑1 (MQ=255)

                                                                          ggTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTc                                                                         >  2:2767094/1‑149 (MQ=255)

                                                                           gTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCa                                                                        <  1:214499/149‑1 (MQ=255)

                                                                           gTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCa                                                                        >  2:1849447/1‑149 (MQ=255)

                                                                           gTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCa                                                                        >  2:2318668/1‑149 (MQ=255)

                                                                            tACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCAc                                                                       <  2:1949705/149‑1 (MQ=255)

                                                                             aCTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCAcc                                                                      >  1:3761654/1‑149 (MQ=255)

                                                                             aCTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCAcc                                                                      >  1:4102314/1‑149 (MQ=255)

                                                                                ttACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAg                                                                   >  1:66080/1‑149 (MQ=255)

                                                                                   cGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTg                                                                >  1:4549184/1‑149 (MQ=255)

                                                                                     ttCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGat                                                              <  1:4922083/149‑1 (MQ=255)

                                                                                     ttCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGat                                                              >  2:133520/1‑149 (MQ=255)

                                                                                          tGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCa                                                         <  1:168767/149‑1 (MQ=255)

                                                                                           gTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCaa                                                        <  1:860305/149‑1 (MQ=255)

                                                                                            tCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCaaa                                                       <  2:4378637/149‑1 (MQ=255)

                                                                                             cTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAAc                                                      <  2:4916431/149‑1 (MQ=255)

                                                                                              ttCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACg                                                     >  2:631844/1‑149 (MQ=255)

                                                                                                cGCCTACTTAAGAAGCGCTCAGACGAGACGGCAAAGTTAAGTCCAGCTGTACTGTACCGAACTGCCATGCACGATCGAGGCAGTCATTCTGTGTAAATTCAATTTTCTGACCGTAGAATGCGCCTTCACCCAGTTGATATTCAAACggg                                                   <  2:2489790/149‑1 (MQ=255)

                                                                                                 gCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGa                                                  <  1:1268547/149‑1 (MQ=255)

                                                                                                   cTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGAtg                                                >  2:1821583/1‑149 (MQ=255)

                                                                                                    tACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGAtgt                                               <  2:757443/149‑1 (MQ=255)

                                                                                                    tACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGAtgt                                               <  2:2011447/149‑1 (MQ=255)

                                                                                                     aCATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGAtgtt                                              <  1:3134946/149‑1 (MQ=255)

                                                                                                       aTAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGAtgttgt                                            <  1:3744062/149‑1 (MQ=255)

                                                                                                        tAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATgttgtt                                           <  1:3809222/149‑1 (MQ=255)

                                                                                                         aagaagCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGttt                                          <  2:3057053/149‑1 (MQ=255)

                                                                                                           gaagCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTttc                                        >  1:1910772/1‑149 (MQ=255)

                                                                                                            aagCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTttct                                       <  1:1589611/149‑1 (MQ=255)

                                                                                                              gcgcTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTttcttc                                     >  2:1081567/1‑149 (MQ=255)

                                                                                                                    agacgagacgGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAgcgc                               >  1:1121225/1‑149 (MQ=255)

                                                                                                                     gacgagacgGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCa                              >  1:2482676/1‑149 (MQ=255)

                                                                                                                     gacgagacgGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCa                              <  2:3434479/149‑1 (MQ=255)

                                                                                                                      acgagacgGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCaa                             >  2:3910208/1‑149 (MQ=255)

                                                                                                                       cgagacgGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAAc                            <  1:113188/149‑1 (MQ=255)

                                                                                                                          gacgGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGc                         >  2:4605360/1‑149 (MQ=255)

                                                                                                                           acgGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGcc                        <  2:3232059/149‑1 (MQ=255)

                                                                                                                               cAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGt                    <  1:3850836/149‑1 (MQ=255)

                                                                                                                                      aaGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTc             <  1:1861240/149‑1 (MQ=255)

                                                                                                                                       aGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCa            >  1:422190/1‑149 (MQ=255)

                                                                                                                                        gTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAg           >  2:3221956/1‑149 (MQ=255)

                                                                                                                                           cAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCAc        <  2:5164653/149‑1 (MQ=255)

                                                                                                                                           cAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCAc        <  2:2515272/149‑1 (MQ=255)

                                                                                                                                           cAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCAc        <  1:4517827/149‑1 (MQ=255)

                                                                                                                                             gCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGa      <  1:4163049/149‑1 (MQ=255)

                                                                                                                                               tgtactgtacCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATc    >  1:2340329/1‑149 (MQ=255)

                                                                                                                                               tgtactgtacCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATc    <  2:392708/149‑1 (MQ=255)

                                                                                                                                                gtactgtacCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATcc   <  1:4724325/149‑1 (MQ=255)

AACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATCCC  >  CP009273/1795234‑1795527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`