BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,796,428	C→A	14.1%	V134F (GTC→TTC)	thrS ←	threonyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,796,428	0	C	A	14.1%	795.5 / 101.5	246	V134F (GTC→TTC)	thrS	threonyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base C (124/87); new base A (18/17); total (142/104)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

GGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGT  >  CP009273/1796281‑1796576

 gCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAcgt                                                                                                                                                    <  1:767432/149‑1 (MQ=255)

     atatCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTa                                                                                                                                                <  1:4900993/149‑1 (MQ=255)

          gACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCTTGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGtttt                                                                                                                                           <  1:2481941/149‑1 (MQ=255)

             atatTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTtct                                                                                                                                        >  2:1734529/1‑149 (MQ=255)

             atatTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTtct                                                                                                                                        <  1:2640390/149‑1 (MQ=255)

             atatTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTtct                                                                                                                                        >  1:1289269/1‑149 (MQ=255)

             atatTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTtct                                                                                                                                        >  2:2802641/1‑149 (MQ=255)

              tatTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTtctc                                                                                                                                       >  2:2967955/1‑149 (MQ=255)

                   ttcATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCaa                                                                                                                                  >  1:400173/1‑149 (MQ=255)

                     cATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGc                                                                                                                                >  2:4175845/1‑149 (MQ=255)

                      aTGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCt                                                                                                                               >  1:4227071/1‑149 (MQ=255)

                       tGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTc                                                                                                                              >  2:1676234/1‑149 (MQ=255)

                        ggAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCa                                                                                                                             >  1:1416767/1‑149 (MQ=255)

                        ggAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCa                                                                                                                             <  2:451034/149‑1 (MQ=255)

                            gTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCa                                                                                                                         >  2:360797/1‑149 (MQ=255)

                            gTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCa                                                                                                                         >  2:3043804/1‑149 (MQ=255)

                                  aCCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCt                                                                                                                   >  1:1733086/1‑149 (MQ=255)

                                  aCCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCt                                                                                                                   >  1:1862666/1‑149 (MQ=255)

                                   ccTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCtt                                                                                                                  >  1:1560218/1‑149 (MQ=255)

                                    cTGGCTTGTCATCATGGGCGATGTTTTCGTCAGGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTtc                                                                                                                 >  1:3587150/1‑149 (MQ=255)

                                    cTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTtc                                                                                                                 <  2:4417089/149‑1 (MQ=255)

                                     tGGCTTTTCATCATGGGCGTTGTTTTAGTCAATAATGGAGTCTTTGTATCTCTCCCCACGGTTGGCGAAATTTTCACGGGCTTCGTTCCATCTGTCGTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTtct                                                                                                                <  1:3724446/149‑1 (MQ=255)

                                       gCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCg                                                                                                              >  1:4753685/1‑149 (MQ=255)

                                        cTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGa                                                                                                             <  1:86218/149‑1 (MQ=255)

                                        cTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCAGCTTCTCGa                                                                                                             >  2:1881499/1‑149 (MQ=255)

                                         ttGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAg                                                                                                            >  2:843617/1‑149 (MQ=255)

                                            tcatcaTGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGc                                                                                                         <  1:1021227/149‑1 (MQ=255)

                                            tcatcaTGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGc                                                                                                         <  1:1547519/149‑1 (MQ=255)

                                             catcatGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCt                                                                                                        <  1:115152/149‑1 (MQ=255)

                                              atcatGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCtt                                                                                                       >  2:3199911/1‑149 (MQ=255)

                                               tcatGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCGTCTCGAGTGCTTc                                                                                                      >  2:2398001/1‑149 (MQ=255)

                                                catGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCg                                                                                                     >  1:1846393/1‑149 (MQ=255)

                                                 atGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGa                                                                                                    >  2:2072537/1‑149 (MQ=255)

                                                  tGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATTACGTCGTAGTTTTTATCAGCAAGCTCATTCATCCGCTTCTCGAGTGCTTCGAc                                                                                                   >  1:3502595/1‑149 (MQ=255)

                                                   gggCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACa                                                                                                  >  1:3016667/1‑149 (MQ=255)

                                                    ggCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACAt                                                                                                 >  1:4132143/1‑149 (MQ=255)

                                                     gCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATc                                                                                                <  1:4498875/149‑1 (MQ=255)

                                                        aTGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTc                                                                                             >  2:1367705/1‑149 (MQ=255)

                                                        aTGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTc                                                                                             <  1:699387/149‑1 (MQ=255)

                                                            tttCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTgg                                                                                         <  2:1855347/149‑1 (MQ=255)

                                                                gTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTa                                                                                     >  2:1166145/1‑149 (MQ=255)

                                                                 tCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTaa                                                                                    >  1:2870190/1‑149 (MQ=255)

                                                                  cAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAAc                                                                                   >  2:1627378/1‑149 (MQ=255)

                                                                   aaGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACg                                                                                  >  1:1169792/1‑149 (MQ=255)

                                                                    aGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGt                                                                                 >  1:1026053/1‑149 (MQ=255)

                                                                       aTGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCg                                                                              >  1:1435269/1‑149 (MQ=255)

                                                                          gagaCTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTc                                                                           >  1:1260990/1‑149 (MQ=255)

                                                                           agaCTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCa                                                                          <  1:4824507/149‑1 (MQ=255)

                                                                                    aGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAcg                                                                 <  1:640777/149‑1 (MQ=255)

                                                                                     gCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAcgt                                                                <  1:1834464/149‑1 (MQ=255)

                                                                                      ctctCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAcgtc                                                               <  2:3646434/149‑1 (MQ=255)

                                                                                       tctcCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAcgtcg                                                              >  1:4239664/1‑149 (MQ=255)

                                                                                        ctcCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAcgtcgt                                                             >  1:2229631/1‑149 (MQ=255)

                                                                                         tcCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGAGCAACGTCGta                                                            >  2:2739712/1‑149 (MQ=255)

                                                                                             cACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGtaataa                                                        <  2:831468/149‑1 (MQ=255)

                                                                                             cACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGtaataa                                                        >  1:3390526/1‑149 (MQ=255)

                                                                                             cACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTCGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGGGCTTCGACATCTTCCTGGGTGAACGGGCGGTCAAGATCAACGTCGtactaa                                                        >  2:3832969/1‑149 (MQ=255)

                                                                                              aCGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATaaa                                                       >  2:956381/1‑149 (MQ=255)

                                                                                              aCGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATaaa                                                       >  2:2263996/1‑149 (MQ=255)

                                                                                               cGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATaaaa                                                      >  1:4309811/1‑149 (MQ=255)

                                                                                                ggTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATaaaaa                                                     <  2:4869899/149‑1 (MQ=255)

                                                                                                      cGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTg                                               <  1:3506103/149‑1 (MQ=255)

                                                                                                        aaaGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTc                                             >  2:2143528/1‑149 (MQ=255)

                                                                                                        aaaGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTc                                             >  2:4700167/1‑149 (MQ=255)

                                                                                                         aaGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCa                                            >  2:3965747/1‑149 (MQ=255)

                                                                                                          aGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCaa                                           >  1:4113761/1‑149 (MQ=255)

                                                                                                             ttCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATaa                                        >  2:419942/1‑149 (MQ=255)

                                                                                                             ttCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATaa                                        >  2:4259282/1‑149 (MQ=255)

                                                                                                             ttCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATaa                                        <  1:1450488/149‑1 (MQ=255)

                                                                                                              tCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAAc                                       >  1:2374924/1‑149 (MQ=255)

                                                                                                                  gcgcTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCggg                                   >  1:5082667/1‑149 (MQ=255)

                                                                                                                   cgcTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGc                                  >  2:3269742/1‑149 (MQ=255)

                                                                                                                   cgcTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGc                                  <  1:3947759/149‑1 (MQ=255)

                                                                                                                      ttCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGa                               <  2:779330/149‑1 (MQ=255)

                                                                                                                      ttCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGa                               >  2:3217356/1‑149 (MQ=255)

                                                                                                                       tCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGAt                              <  2:1560218/149‑1 (MQ=255)

                                                                                                                       tCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGAt                              <  1:1676234/149‑1 (MQ=255)

                                                                                                                       tCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGAt                              <  1:3199911/149‑1 (MQ=255)

                                                                                                                       tCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGAt                              <  2:1562556/149‑1 (MQ=255)

                                                                                                                            ccAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCa                         >  2:891573/1‑149 (MQ=255)

                                                                                                                            ccAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGACa                         >  1:992298/1‑149 (MQ=255)

                                                                                                                             cAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCAt                        >  2:2418171/1‑149 (MQ=255)

                                                                                                                              aGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCAtt                       >  1:1936440/1‑149 (MQ=255)

                                                                                                                               gCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCCttt                      >  1:3096847/1‑149 (MQ=255)

                                                                                                                                cTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCAtttt                     <  1:4798106/149‑1 (MQ=255)

                                                                                                                                  gACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTGCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTgg                   >  2:4379739/1‑149 (MQ=255)

                                                                                                                                   aCTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGt                  <  2:3698399/149‑1 (MQ=255)

                                                                                                                                    cTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTa                 <  2:1026010/149‑1 (MQ=255)

                                                                                                                                       tcttctTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGc              >  1:4406952/1‑149 (MQ=255)

                                                                                                                                       tcttctTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGc              >  2:3079634/1‑149 (MQ=255)

                                                                                                                                        cttcttAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCg             <  1:909049/149‑1 (MQ=255)

                                                                                                                                         ttcttAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCgg            <  1:429709/149‑1 (MQ=255)

                                                                                                                                          tcttAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGc           >  1:2761507/1‑149 (MQ=255)

                                                                                                                                              aaTGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCaaa       >  1:2802714/1‑149 (MQ=255)

                                                                                                                                               aTGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAg      >  2:5011777/1‑149 (MQ=255)

                                                                                                                                                tGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAgt     <  1:3328628/149‑1 (MQ=255)

                                                                                                                                                 gACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAgtt    >  2:465163/1‑149 (MQ=255)

                                                                                                                                                 gACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAgtt    >  2:2966319/1‑149 (MQ=255)

                                                                                                                                                  aCGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAgttg   <  2:3284564/149‑1 (MQ=255)

                                                                                                                                                   cgtcgtAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAgttgt  <  1:2608910/149‑1 (MQ=255)

GGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGT  >  CP009273/1796281‑1796576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`