Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,823,835 A→C 7.5% W53G (TGG→GGG)  astD ← succinylglutamic semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,823,8350AC 7.5% 766.0 / 2.3 289W53G (TGG→GGG) astDsuccinylglutamic semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (195/60);  new base C (6/26);  total (201/88)
Fisher's exact test for biased strand distribution p-value = 2.32e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

ATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCAT  >  CP009273/1823687‑1823983
                                                                                                                                                     |                                                                                                                                                    
atcatcGCCGTCACTTCGGGTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCa                                                                                                                                                      <  2:5172768/149‑1 (MQ=255)
   atcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAgcg                                                                                                                                                   >  2:4522803/1‑149 (MQ=255)
      gCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATACTCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCgg                                                                                                                                                >  2:2775190/1‑149 (MQ=255)
      gCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCgg                                                                                                                                                >  1:2030749/1‑149 (MQ=255)
       ccGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCCGCGCGGa                                                                                                                                               >  1:3541445/1‑149 (MQ=255)
         gTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGaaa                                                                                                                                             >  1:4126639/1‑149 (MQ=255)
            aCTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAAcgc                                                                                                                                          >  1:3332346/1‑149 (MQ=255)
                     gCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAACCGCCGCACGGGc                                                                                                                                 >  2:3759461/1‑149 (MQ=255)
                        gCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGc                                                                                                                              >  1:2948592/1‑149 (MQ=255)
                          ttCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGccc                                                                                                                            >  1:4115917/1‑149 (MQ=255)
                            cccAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGa                                                                                                                          >  2:4369602/1‑149 (MQ=255)
                             ccAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGAc                                                                                                                         >  1:3294938/1‑149 (MQ=255)
                             ccAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGAc                                                                                                                         >  2:2315706/1‑149 (MQ=255)
                             ccAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGAc                                                                                                                         >  1:5112230/1‑149 (MQ=255)
                              cAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGACACGCCGCACGGGCTGCCCGACa                                                                                                                        >  2:2438877/1‑149 (MQ=255)
                              cAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACa                                                                                                                        >  2:3970203/1‑149 (MQ=255)
                                  gcgGATTACCCGTTTCTCTGGCAATAATCGCGGTTAAGTCGGATTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGcc                                                                                                                    <  2:4304641/149‑1 (MQ=255)
                                      cTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCt                                                                                                                >  2:104403/1‑149 (MQ=255)
                                        tACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCg                                                                                                              >  2:2543930/1‑149 (MQ=255)
                                        tACCCGTTTCTCTGGAAATAATCGGGTTTAATTGGGTTTTTTTGCTTTCCAGCCTTGCGGAAACGGTTTCGACACCGGATTGACTTTCACAAAATT‑AAAGCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCg                                                                                                              <  1:1690098/149‑1 (MQ=255)
                                          cccGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGAc                                                                                                            <  2:4428724/149‑1 (MQ=255)
                                           ccGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGAcc                                                                                                           >  1:2436151/1‑149 (MQ=255)
                                             gTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTg                                                                                                         <  2:409771/149‑1 (MQ=255)
                                              tttCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGa                                                                                                        >  1:3137587/1‑149 (MQ=255)
                                                 ctcgGGAAATAATCGGGGTTATTTCGGCTTTTTTGCTTTCCAGCTGGGCGCAAATGCGTTCCCCACGGGCATGACGTTCAGCAAAGT‑AAAGCCGCGCCCCGCGCGCAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcg                                                                                                     <  2:3910720/149‑1 (MQ=255)
                                                    tGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggca                                                                                                  >  2:3088116/1‑149 (MQ=255)
                                                    tGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggca                                                                                                  >  1:4060660/1‑149 (MQ=255)
                                                     ggCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcat                                                                                                 >  2:982545/1‑149 (MQ=255)
                                                     ggCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcat                                                                                                 >  2:3578045/1‑149 (MQ=255)
                                                      gCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatc                                                                                                >  1:856346/1‑149 (MQ=255)
                                                       cAATACTCGCGGTTAATTCGGCTTTCTTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTACAGCAAATG‑AACGCCGCGCCCAGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcg                                                                                               <  2:564868/149‑1 (MQ=255)
                                                         ataatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggc                                                                                             >  2:1960399/1‑149 (MQ=255)
                                                         ataatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggc                                                                                             >  2:3819386/1‑149 (MQ=255)
                                                               ggggTTAATGGGGATTTTTTGCTTGCCTGCAGTGCGTAAACGCGTTCGAAAACGGAATGCCGTTC‑CCAAATGAAAAGCCGCGCCCAGCGCGCAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAtt                                                                                       <  1:2329241/147‑1 (MQ=255)
                                                               gcgGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAtt                                                                                       >  2:796152/1‑149 (MQ=255)
                                                                   ttAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGAAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcct                                                                                   <  2:1651702/149‑1 (MQ=255)
                                                                     aaTTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttg                                                                                 >  1:3404630/1‑149 (MQ=255)
                                                                      aTTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgc                                                                                >  1:3215467/1‑149 (MQ=255)
                                                                       ttCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgcc                                                                               >  2:4852683/1‑149 (MQ=255)
                                                                        tggggttttttGCTTTCCCGCTGTGCGGCAAAGGGTTCGACACCGGAATGACGTTCCGAAAATG‑AAAGCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCa                                                                              <  1:1978635/144‑1 (MQ=255)
                                                                        tCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCa                                                                              >  1:1109879/1‑149 (MQ=255)
                                                                            cTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAac                                                                          >  2:3627595/1‑149 (MQ=255)
                                                                            atttATTGATTTCCTGCTGTGCGGCAACGCTTTCGACACCGGATTTACTTTCAGAAATTC‑AAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGACATCGGCATCATTGCCTTGCCATAac                                                                          <  1:756622/148‑1 (MQ=255)
                                                                                tttGGTTTCCAGCAGGGCGGCAACGCGTTCGACACCGGCATGACGTTCAGCAATTG‑AAAGCCGCGCCCAGCGCGTAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCt                                                                      <  2:475395/148‑1 (MQ=255)
                                                                                     tttCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGccc                                                                 >  1:2807847/1‑149 (MQ=255)
                                                                                     tttCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGccc                                                                 >  2:3017937/1‑149 (MQ=255)
                                                                                          agcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAc                                                            >  2:2922962/1‑149 (MQ=255)
                                                                                           gcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAcc                                                           >  1:3980844/1‑149 (MQ=255)
                                                                                              gTGCGGCACAGGGTGCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAGTGCCTTGCCATAACACCTCGCCCGATACCGGa                                                        <  2:3512632/149‑1 (MQ=255)
                                                                                              gTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGa                                                        >  1:4316708/1‑149 (MQ=255)
                                                                                               tGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGAt                                                       >  1:548603/1‑149 (MQ=255)
                                                                                                  ggCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTAc                                                    >  1:4965228/1‑149 (MQ=255)
                                                                                                     aaaGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCt                                                 >  2:3866138/1‑149 (MQ=255)
                                                                                                     aaaGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCt                                                 >  1:1305482/1‑149 (MQ=255)
                                                                                                      aaGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCtt                                                >  1:4915759/1‑149 (MQ=255)
                                                                                                       aGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTc                                               >  1:267908/1‑149 (MQ=255)
                                                                                                        gCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTca                                              >  1:2200677/1‑149 (MQ=255)
                                                                                                         cGTTCGAAAACGGATTGAGGTTCAGAAAATG‑AAAGCCGCGCCCAGCGCGTAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTcac                                             <  1:4712215/149‑1 (MQ=255)
                                                                                                          gTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTcaca                                            >  2:845271/1‑149 (MQ=255)
                                                                                                             cGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACAcgc                                         <  2:4195704/149‑1 (MQ=255)
                                                                                                               aCAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGa                                       >  1:1445973/1‑149 (MQ=255)
                                                                                                               aCAACGGCATGACGATCAGCAAATG‑AAAGCCGCGCCCACCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCACGACCTGAGCGGCAACGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGa                                       >  1:3835635/1‑149 (MQ=255)
                                                                                                                cAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGAt                                      >  1:868558/1‑149 (MQ=255)
                                                                                                                 aaCGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATg                                     >  1:1152684/1‑149 (MQ=255)
                                                                                                                 aaCGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATg                                     >  1:1152700/1‑149 (MQ=255)
                                                                                                                  acggattGACGTTCAGAAAATT‑AACGCCGCGCCCAGCGCGTAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATgc                                    <  2:2200677/143‑1 (MQ=255)
                                                                                                                   cGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATgcg                                   >  1:3046029/1‑149 (MQ=255)
                                                                                                                    ggCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATgcgc                                  >  1:3028226/1‑149 (MQ=255)
                                                                                                                    agCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATgcgc                                  <  2:4913858/148‑1 (MQ=255)
                                                                                                                     gCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGcc                                 >  2:5003235/1‑149 (MQ=255)
                                                                                                                      cATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGccc                                <  2:1608924/149‑1 (MQ=255)
                                                                                                                      cATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGccc                                <  2:1128316/149‑1 (MQ=255)
                                                                                                                       ttGACGTTCAGCAAATG‑AATGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCt                               <  2:2402164/148‑1 (MQ=255)
                                                                                                                        tGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTg                              <  2:2988513/149‑1 (MQ=255)
                                                                                                                           cGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGcc                           >  2:2167134/1‑149 (MQ=255)
                                                                                                                             ttCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCg                         >  2:2532088/1‑149 (MQ=255)
                                                                                                                              tccgaaaaTG‑AACGCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGt                        <  1:2775027/144‑1 (MQ=255)
                                                                                                                              tCAGCAATTG‑AAAGCCGCGCCCCGCGCGTAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGt                        <  1:3805507/149‑1 (MQ=255)
                                                                                                                              tCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGt                        >  1:2164665/1‑149 (MQ=255)
                                                                                                                                    aaTG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCa                  >  1:1091838/1‑149 (MQ=255)
                                                                                                                                    aaTG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCa                  <  2:1543139/149‑1 (MQ=255)
                                                                                                                                    aaTG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCa                  >  2:355130/1‑149 (MQ=255)
                                                                                                                                      tG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGt                >  1:4225475/1‑149 (MQ=255)
                                                                                                                                       g‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTc               <  2:2653319/149‑1 (MQ=255)
                                                                                                                                       g‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTc               <  2:384187/149‑1 (MQ=255)
                                                                                                                                         aaaGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCa              >  2:3695377/1‑149 (MQ=255)
                                                                                                                                          aaGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCAc             >  1:2071675/1‑149 (MQ=255)
                                                                                                                                          aaGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCAc             >  1:4437904/1‑149 (MQ=255)
                                                                                                                                           aGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCAcc            >  2:4271534/1‑149 (MQ=255)
                                                                                                                                           aGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCAcc            >  1:605507/1‑149 (MQ=255)
                                                                                                                                             ccGCGCCCAGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGt          <  2:4145572/149‑1 (MQ=255)
                                                                                                                                             ccGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGt          <  2:4697616/149‑1 (MQ=255)
                                                                                                                                              cgcgCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGtt         <  1:4628026/149‑1 (MQ=255)
                                                                                                                                                cgcCCGGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTaa       <  1:4859309/149‑1 (MQ=255)
                                                                                                                                                 gcCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAAt      >  1:111971/1‑149 (MQ=255)
                                                                                                                                                 gcCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAAt      >  1:1507606/1‑149 (MQ=255)
                                                                                                                                                   ccAGCGCTTACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATcc    <  1:5043211/149‑1 (MQ=255)
                                                                                                                                                   ccAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATcc    <  1:1604597/149‑1 (MQ=255)
                                                                                                                                                     aGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCAt  >  2:4790537/1‑149 (MQ=255)
                                                                                                                                                     aGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCAt  <  2:2070687/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATG‑AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCAT  >  CP009273/1823687‑1823983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑