BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,863,933	A→C	6.3%	K241T (AAG→ACG)	yeaH →	UPF0229 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,863,933	0	A	C	6.3%	938.8 / 4.8	325	K241T (AAG→ACG)	yeaH	UPF0229 family protein
Reads supporting (aligned to +/- strand): ref base A (145/149); new base C (2/29); total (147/178)
Fisher's exact test for biased strand distribution p-value = 1.55e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 331 total aligned reads displayed.

GAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTA  >  CP009273/1863785‑1864081

gAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGaa                                                                                                                                                      <  2:4755479/149‑1 (MQ=255)

 aaGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAg                                                                                                                                                     >  2:2820070/1‑149 (MQ=255)

     gaATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCAGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACACGTCCCTATTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGc                                                                                                                                                 >  2:2610083/1‑149 (MQ=255)

       aTTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGATACAAGAACTACGAGACGCGGccc                                                                                                                                               >  2:2492191/1‑149 (MQ=255)

       aTTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGAAAAGAAATTGAATAATTACGTGCCAAAATTGAACGCGTCCATTTTTTTGACACCTTCTATTTACGTTACAATAACTACGAGAAGCGGccc                                                                                                                                               <  2:1550978/149‑1 (MQ=255)

         ttGGCAATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGAAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGa                                                                                                                                             <  2:2997798/149‑1 (MQ=255)

          tGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGATTTACGTGCCAAAATTTAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGACGCGGCCCGAt                                                                                                                                            <  2:1609127/149‑1 (MQ=255)

          tGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGAt                                                                                                                                            >  1:3513684/1‑149 (MQ=255)

           ggCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATc                                                                                                                                           >  1:5031705/1‑149 (MQ=255)

                  atcaGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTccag                                                                                                                                    >  1:1031781/1‑149 (MQ=255)

                  atcaGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTccag                                                                                                                                    >  1:1458534/1‑149 (MQ=255)

                   tcaGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTccagc                                                                                                                                   >  2:1021971/1‑149 (MQ=255)

                      gCAACAGTGAACCTGCGCAACTGCTGGATGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTTTTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTccagccag                                                                                                                                <  2:3260996/149‑1 (MQ=255)

                        aaCAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGc                                                                                                                              >  2:4095819/1‑149 (MQ=255)

                         aCAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCa                                                                                                                             >  2:3355965/1‑149 (MQ=255)

                            gTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTg                                                                                                                          >  1:1082791/1‑149 (MQ=255)

                             tGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGa                                                                                                                         >  2:1325761/1‑149 (MQ=255)

                               aaCCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATg                                                                                                                       >  1:3011100/1‑149 (MQ=255)

                                  cTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGttt                                                                                                                    <  2:3292955/149‑1 (MQ=255)

                                     cgcAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGc                                                                                                                 >  1:1770946/1‑149 (MQ=255)

                                      gcAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCAAAAATTGAACGCGTCCCTTTTATTGACACCTTCTATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGcc                                                                                                                <  1:3415386/149‑1 (MQ=255)

                                      gaaaCTGCTGTAATAGTAACGTCTGCGCAAATAATTTGAAGATTTAGGTGCAAAAATTTAACGCGTCCCTTTTATTGACACCTTGTATTTACGTTACAATAACTACTAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGcc                                                                                                                <  1:4271411/147‑1 (MQ=255)

                                       cacctgctgGCAGAGGAACGTCTGCGAAAAGAAAGTCCATAATTACGTGCAAAAATTGACCGCGTCCATTTTTTTGACACATTCGATTTACGTTACAACAACTACGACACGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCt                                                                                                               <  1:2848321/146‑1 (MQ=255)

                                         cctgctgTAAGAGGAACGTATGCTCAAAGAAATTGCAGAATTACGTCCCAAAATTCACCGCGCCCCTTTTATTGACACCTTCTATTTACGTAACAAGAACTCCGAGACGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGa                                                                                                             <  1:4924696/148‑1 (MQ=255)

                                           tgctgGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATg                                                                                                           >  2:2140366/1‑149 (MQ=255)

                                                 aaGCGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTCTTGACACCTTCGATTTACGTTACAAGAACTACGAGACGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGtt                                                                                                     <  2:3773226/149‑1 (MQ=255)

                                                   gagGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACgggtc                                                                                                   >  2:1911366/1‑145 (MQ=255)

                                                   gagGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTc                                                                                                   >  1:4535348/1‑149 (MQ=255)

                                                    agGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTcc                                                                                                  >  2:4905602/1‑149 (MQ=255)

                                                      gaccGTCTGCGCAAAGAATTGGCAGACTTCCGTGCAAAAATTGACCGCGGCCCTTTTCTTCACACCTTCGTTTTACGTTACAAGACCTCCGAGACGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCgg                                                                                                <  1:536894/146‑1 (MQ=255)

                                                       aaCGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGt                                                                                               >  2:4999575/1‑149 (MQ=255)

                                                        aCGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGtt                                                                                              <  2:1666876/149‑1 (MQ=255)

                                                        aCGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGtt                                                                                              >  2:3691075/1‑149 (MQ=255)

                                                           tCTGCGAAAATAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATGGACACCTTCTATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCaa                                                                                           <  2:980499/149‑1 (MQ=255)

                                                             tGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATg                                                                                         >  2:3808592/1‑149 (MQ=255)

                                                               cgcAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGa                                                                                       <  2:748047/149‑1 (MQ=255)

                                                                 aaaaGAATTTGCAGAATTACGTGCCAAAATTGACCGCGTCCCTTTTTTTGACACCTTCGATTTACGTTACACGAACTACGAGACGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATc                                                                                     <  1:3353709/148‑1 (MQ=255)

                                                                  aaaGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCa                                                                                    >  1:2533061/1‑149 (MQ=255)

                                                                  aaaGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCa                                                                                    >  1:1310887/1‑149 (MQ=255)

                                                                   aataaaTTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCaa                                                                                   <  2:4396250/146‑1 (MQ=255)

                                                                    aGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAAt                                                                                  >  2:5140385/1‑149 (MQ=255)

                                                                    aGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAAt                                                                                  >  1:3268509/1‑149 (MQ=255)

                                                                    aGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAAt                                                                                  <  2:5031705/149‑1 (MQ=255)

                                                                        cctGCAGAATTCCGTGCCAAACTTGAACGCGTCCCTTTTCTTGCCCCCTTCGATTTACGTTACAAGAACTACGAGACGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCAc                                                                              <  2:3111856/147‑1 (MQ=255)

                                                                        aTTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCAc                                                                              <  2:620102/149‑1 (MQ=255)

                                                                         ttGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACt                                                                             <  2:1097840/149‑1 (MQ=255)

                                                                          tGCAGATTTACGTGCAAAAATTGAACGCTTCCCTTTTATTGACACCTTCTATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTa                                                                            <  2:4769755/149‑1 (MQ=255)

                                                                            cataaTTACGTGCAAAAATTGAACGCGTCCATTTTATTGACACCTTCGATTTACGTTAAAAGAACTACGATAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTaaa                                                                          <  1:799906/146‑1 (MQ=255)

                                                                            cAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTaaa                                                                          >  1:4050524/1‑149 (MQ=255)

                                                                             atatttACGTGCAAAATTTGACCGCGTCCATTTTTTTGACACCTTCGATTTAGGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAg                                                                         <  1:4162193/145‑1 (MQ=255)

                                                                             aGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAg                                                                         >  1:4510349/1‑149 (MQ=255)

                                                                               aaTTACTTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCTATTTACTTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGat                                                                       <  1:1978320/149‑1 (MQ=255)

                                                                               aaTTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAgtt                                                                       >  1:2130392/1‑147 (MQ=255)

                                                                                aTTACGTGCAAAAATTGAACGCGTCCCTTTTTTTGACACCTTCTTTTTACGTTACAAGAACTACGAGACGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGata                                                                      <  1:4968504/149‑1 (MQ=255)

                                                                                aTTACGTGCAAAAATTGAACGCGTCCCTTTTATTGACACCTTCTATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGata                                                                      <  2:3909275/149‑1 (MQ=255)

                                                                                 ttACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGatat                                                                     >  2:3886296/1‑149 (MQ=255)

                                                                                 ttACGTGCAAAAATTTAACGCGTCCCTTTTATTGACACCTTCTATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGatat                                                                     <  1:1505294/149‑1 (MQ=255)

                                                                                       gCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGTTTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTaa                                                               <  1:1219543/149‑1 (MQ=255)

                                                                                         cAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTAAAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGc                                                             <  2:1884227/149‑1 (MQ=255)

                                                                                         cAAAATTGAACGCGTCCATTTTATTGACACCTTCGATTTACGTTAAAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGc                                                             <  2:141286/149‑1 (MQ=255)

                                                                                         aaataTTGACCGCGTCCTTTTTATTCACACCTTATTTTTACTTTAAAATAACAACTACCCGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGc                                                             <  1:2602884/145‑1 (MQ=255)

                                                                                         aaaaaTTGAACGCGTCCATTTTATTGACACCTTCTTTTTAGGTTAAAATAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGc                                                             <  1:694114/148‑1 (MQ=255)

                                                                                           aaaTTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGt                                                           >  2:2396350/1‑149 (MQ=255)

                                                                                           aaaTTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGt                                                           >  2:4606674/1‑149 (MQ=255)

                                                                                           aaaTTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGt                                                           >  1:2061032/1‑149 (MQ=255)

                                                                                             aTTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTCAAAGAACTACTAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGttt                                                         <  2:1113545/149‑1 (MQ=255)

                                                                                              ttGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGtttt                                                        >  1:4774097/1‑149 (MQ=255)

                                                                                               tGAACGCGTCCTTTTTATTGAAACCTTCTTTTTACTTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGttttt                                                       <  1:1349630/149‑1 (MQ=255)

                                                                                                gAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTta                                                      >  1:659989/1‑149 (MQ=255)

                                                                                                    gcgTCCCTTTTATTGACACCTTCTATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATAtt                                                  <  2:46455/149‑1 (MQ=255)

                                                                                                    gcgTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATAtt                                                  <  2:824267/149‑1 (MQ=255)

                                                                                                     cgTCCCTTTTATTGACACCTTCGATTTACGTTACAAGACCTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTc                                                 <  2:4531835/149‑1 (MQ=255)

                                                                                                     cgTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTc                                                 <  1:1666449/149‑1 (MQ=255)

                                                                                                     cgTCCCTTTTATTCACACCTTCCATTTACGTTACAAGAACCACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTc                                                 <  2:1661697/149‑1 (MQ=255)

                                                                                                       tCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTctg                                               >  1:1114890/1‑149 (MQ=255)

                                                                                                         ccTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTctgct                                             >  1:2797685/1‑149 (MQ=255)

                                                                                                         ccTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCACTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTctgct                                             >  2:2239567/1‑149 (MQ=255)

                                                                                                           ttttATTTACACCTTCGTTTTACTTTACAAGAACAACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGt                                           <  1:1909390/149‑1 (MQ=255)

                                                                                                           ttttATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGt                                           >  1:2657569/1‑149 (MQ=255)

                                                                                                            tttatttaCACATTCGTTTTACGTTACAAGACCTCCGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTa                                          <  1:3698477/149‑1 (MQ=255)

                                                                                                              tATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATc                                        >  2:3470931/1‑149 (MQ=255)

                                                                                                              tATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATc                                        >  2:359120/1‑149 (MQ=255)

                                                                                                                     acCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCt                                 <  2:2024875/149‑1 (MQ=255)

                                                                                                                      ccTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTc                                <  2:3712811/149‑1 (MQ=255)

                                                                                                                         tCGATTTACGTTACAAGAACAACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTcagc                             <  2:3610515/149‑1 (MQ=255)

                                                                                                                          ctaTTTACGTTAAAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTcagca                            <  1:628312/147‑1 (MQ=255)

                                                                                                                          cGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTcagca                            >  1:4605802/1‑149 (MQ=255)

                                                                                                                            aTTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGa                          >  1:95108/1‑149 (MQ=255)

                                                                                                                             tttACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGaa                         <  2:4510344/149‑1 (MQ=255)

                                                                                                                             tttACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGaa                         <  2:1430876/149‑1 (MQ=255)

                                                                                                                                aCGTTACAATAACTCCGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGt                      <  1:1403906/149‑1 (MQ=255)

                                                                                                                                     aCAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAg                 >  2:616113/1‑149 (MQ=255)

                                                                                                                                            ctccGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTgg          <  1:3643454/146‑1 (MQ=255)

                                                                                                                                            cTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTgg          >  2:1848103/1‑149 (MQ=255)

                                                                                                                                              aCGAGACGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGaa        <  2:1808236/149‑1 (MQ=255)

                                                                                                                                               cGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAg       <  1:2191910/149‑1 (MQ=255)

                                                                                                                                                gagaAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGt      >  1:127419/1‑149 (MQ=255)

                                                                                                                                                 agaAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTc     >  1:4368212/1‑149 (MQ=255)

                                                                                                                                                    aGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTCTAAGAACGTGGAAGTCGta  >  1:4810100/1‑149 (MQ=255)

                                                                                                                                                    aGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGta  <  1:3691075/149‑1 (MQ=255)

GAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTGACACCTTCGATTTACGTTACAAGAACTACGAGAAGCGGCCCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTA  >  CP009273/1863785‑1864081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`