Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,303,607 A→C 5.6% L93R (CTG→CGG)  ada ← fused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,303,6070AC 5.6% 943.8 / 6.5 353L93R (CTG→CGG) adafused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (148/168);  new base C (30/2);  total (178/175)
Fisher's exact test for biased strand distribution p-value = 9.28e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 370 total aligned reads displayed.

TTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGG  >  CP009273/2303459‑2303754
                                                                                                                                                    |                                                                                                                                                   
ttCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCcagca                                                                                                                                                     >  2:795984/1‑149 (MQ=255)
     gcAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGa                                                                                                                                                <  1:4076910/149‑1 (MQ=255)
     gcAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCGGTTCCCGCAGTCGa                                                                                                                                                >  2:1512675/1‑149 (MQ=255)
       aaaCGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTACAGCAGTCGacc                                                                                                                                              >  1:5043421/1‑148 (MQ=255)
        aaCGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGacac                                                                                                                                             <  1:1869520/149‑1 (MQ=255)
            gCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTTGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGt                                                                                                                                         >  2:393071/1‑149 (MQ=255)
                gcgcgcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGt                                                                                                                                     <  1:3082838/149‑1 (MQ=255)
                  gcgcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGa                                                                                                                                   <  2:1853538/149‑1 (MQ=255)
                  gcgcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGa                                                                                                                                   <  2:3397809/149‑1 (MQ=255)
                  gcgcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTACAGCAGTCGAAACGCGTGGGTGa                                                                                                                                   >  2:2278390/1‑149 (MQ=255)
                   cgcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGAt                                                                                                                                  <  1:1261385/149‑1 (MQ=255)
                    gcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGAtt                                                                                                                                 <  1:1725679/149‑1 (MQ=255)
                    gcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGAtt                                                                                                                                 >  2:3695938/1‑149 (MQ=255)
                    gcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTACTGTTCCAGCAGTCGACACGCGTGGGTGAtt                                                                                                                                 >  2:496964/1‑149 (MQ=255)
                     ccAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGAttt                                                                                                                                <  2:3039964/149‑1 (MQ=255)
                       aGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTa                                                                                                                              <  2:1701276/149‑1 (MQ=255)
                       aGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCGGTTCCAGCAGTCGACACGCGTGGGTGATTTTa                                                                                                                              >  1:4643251/1‑149 (MQ=255)
                       aGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTGTCCTGTTCCAGCAGGCGCCACGCGTGGGTGATTTTa                                                                                                                              >  1:4691498/1‑149 (MQ=255)
                        ggCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTAt                                                                                                                             >  1:2009049/1‑149 (MQ=255)
                        ggCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTAt                                                                                                                             <  2:631696/149‑1 (MQ=255)
                           cTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCa                                                                                                                          >  2:5174994/1‑149 (MQ=255)
                           cTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCa                                                                                                                          >  2:2391102/1‑149 (MQ=255)
                            tGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTATCCaa                                                                                                                         >  1:826341/1‑149 (MQ=255)
                            tGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTATCCaa                                                                                                                         >  1:3416458/1‑149 (MQ=255)
                            tGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCGGTTCCAGCCGGCGACCCGCGTGGGTGATTTTATCCaa                                                                                                                         >  1:4191696/1‑149 (MQ=255)
                            tGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTACTGTTCCAGCAGTCGACACGCGGGGGTGATTTTATACaa                                                                                                                         >  2:90171/1‑149 (MQ=255)
                             gTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAAc                                                                                                                        >  2:1111886/1‑149 (MQ=255)
                                    ggCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCCCCCCTCGACACGCGTGGGTGGTTTTATCCAAACGATGtt                                                                                                                 >  2:3499705/1‑149 (MQ=255)
                                       tttAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCt                                                                                                              <  2:2009049/149‑1 (MQ=255)
                                       tttAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATACAACCGATGTTGCt                                                                                                              >  1:1657920/1‑149 (MQ=255)
                                          aGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTACTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGAAGGTGCTggg                                                                                                           >  2:4391019/1‑149 (MQ=255)
                                              gTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCAtt                                                                                                       >  2:4211919/1‑149 (MQ=255)
                                               tCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGGCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTg                                                                                                      >  2:1636635/1‑149 (MQ=255)
                                               tCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTGTCCTGTTCCCTCAGTCGACACCCGGGGGTGATTTTATCCAACCGAGGTTTCTGGGCCTTg                                                                                                      >  1:3688370/1‑149 (MQ=255)
                                                  ttCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCt                                                                                                   <  2:3335751/149‑1 (MQ=255)
                                                    ccGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCttt                                                                                                 <  1:4949853/149‑1 (MQ=255)
                                                    ccGGTAGTCGCTTTAAACAACCGAGGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCttt                                                                                                 <  1:1019686/149‑1 (MQ=255)
                                                     cGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCtttt                                                                                                <  2:813311/149‑1 (MQ=255)
                                                        tAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCt                                                                                             <  2:2039531/149‑1 (MQ=255)
                                                        tAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCt                                                                                             <  2:4091192/149‑1 (MQ=255)
                                                        tAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCt                                                                                             <  2:5109782/149‑1 (MQ=255)
                                                         aGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTg                                                                                            >  2:2065050/1‑149 (MQ=255)
                                                         aGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATACAACCGATTTTGCTGGGCCTTGGCTTTTTATg                                                                                            >  1:2341916/1‑149 (MQ=255)
                                                         aGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATACAACCGATGTTGCTGGGCATTGGCTTTTTCTg                                                                                            >  2:1016364/1‑149 (MQ=255)
                                                         aGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTACTGTTACAGCAGTCGACACCCGGGGGTGATTTTTTAAAAACGATTTTTCTGGGGCTTGGCTTTTTATg                                                                                            >  1:1336729/1‑149 (MQ=255)
                                                          gTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTgg                                                                                           <  2:5071336/149‑1 (MQ=255)
                                                          gTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGAGTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTgg                                                                                           >  2:5137024/1‑149 (MQ=255)
                                                           tCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGc                                                                                          >  1:2469933/1‑149 (MQ=255)
                                                           tCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGc                                                                                          <  2:1825717/149‑1 (MQ=255)
                                                           tCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGGGGGTGATTTTATCCAACCGATGTTGGTGGGCATTGGCTTTTTCTTGc                                                                                          >  2:4768487/1‑149 (MQ=255)
                                                              cTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGa                                                                                       <  2:526997/149‑1 (MQ=255)
                                                                   aacaacCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACCCGCGGGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTATGGCTGTCAACg                                                                                  >  1:3098310/1‑149 (MQ=255)
                                                                   aacaacCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTACAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCCTTGGCTTTTTCTGGCTGACAACg                                                                                  >  1:147645/1‑149 (MQ=255)
                                                                     caacCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACACCGtt                                                                                <  2:725246/149‑1 (MQ=255)
                                                                       acCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTg                                                                              <  1:4496916/149‑1 (MQ=255)
                                                                           aTGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAgg                                                                          >  2:4482309/1‑149 (MQ=255)
                                                                           aTGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTAGCAACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCAATGGCTTTTTCTGGCTGACAACGTTTGCAgg                                                                          >  2:1912907/1‑149 (MQ=255)
                                                                           aTGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTACTGGTCCAGCCGTCGACACGCGTGGGTGATTTTATACAACCCATGTTGCTGGGCATTGGCTTTTTCTTGCTGACCACGTTTGCAgg                                                                          >  1:1430726/1‑149 (MQ=255)
                                                                            tGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTGCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCAATGGCTTTTTCTGGGTGACAACGTTTGCAggg                                                                         >  1:1135118/1‑149 (MQ=255)
                                                                            tGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCCggg                                                                         >  1:1209660/1‑149 (MQ=255)
                                                                            tGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAggg                                                                         >  2:3885978/1‑149 (MQ=255)
                                                                            tGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTACAGCAGTCGACACGCGTGGGTGATTTTATCCAAACGATGTTGCTGGGCATTGGCTTTTTCTGGGTGAAAAAGTTTGCAggg                                                                         >  1:2304149/1‑149 (MQ=255)
                                                                              tAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGc                                                                       >  1:1617508/1‑149 (MQ=255)
                                                                                  tGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGaaa                                                                   >  2:2514009/1‑149 (MQ=255)
                                                                                  tGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGaaa                                                                   <  2:4649784/149‑1 (MQ=255)
                                                                                   gAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAg                                                                  <  1:3928690/149‑1 (MQ=255)
                                                                                    aaaTGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTTTACAACCGATGTTGCTGGGCCTTGGCTTTTTATGGCTGTCAACGTTTGCAGGGGCGAAAGc                                                                 >  1:2600519/1‑149 (MQ=255)
                                                                                        ggACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAgc                                                             <  1:486199/149‑1 (MQ=255)
                                                                                           cTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAgcggc                                                          >  2:4592131/1‑149 (MQ=255)
                                                                                           cTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAgcggc                                                          <  2:426273/149‑1 (MQ=255)
                                                                                            tCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAgcggcg                                                         <  1:3444738/149‑1 (MQ=255)
                                                                                              aTCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAg                                                       <  2:1366892/149‑1 (MQ=255)
                                                                                                cGCCACCTGGTCGGCTATGGCTTCCAGCGTTACTGGCGTTTCCTGTTCCTGCAGTCGACACGCGTGGGTTTTTTTATCCAACCGATGTTGGTGGGATTTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCTAAAGCCAGCGGCGAGTg                                                     <  1:778585/149‑1 (MQ=255)
                                                                                                  ccACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGcc                                                   <  2:4307307/149‑1 (MQ=255)
                                                                                                  ccACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGGGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGcc                                                   <  2:4551869/149‑1 (MQ=255)
                                                                                                  ccACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTACTGTTCCAGCCCTCGACACGCGTGGGTGATTTTATACAACCGATGTTTCTGGGCATTTGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGcc                                                   >  1:2741362/1‑149 (MQ=255)
                                                                                                   cACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCCACCGATGTTGCTGGGCCTTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGGGCCt                                                  >  2:3382473/1‑149 (MQ=255)
                                                                                                   cACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCTTTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCTAAAGCCAGCGGCGAGTGCCt                                                  <  2:1530050/149‑1 (MQ=255)
                                                                                                   cACCGGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGGCGACACGCGTGGGTGATTTTATCCACCCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCt                                                  <  2:4839223/149‑1 (MQ=255)
                                                                                                     ccTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTACGGTTCCAGCAGTCGACCCGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCCTTGGCTTTTTCTGGCTGACACCGGTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCg                                                >  2:3032749/1‑149 (MQ=255)
                                                                                                      cTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGc                                               <  2:377812/149‑1 (MQ=255)
                                                                                                        ggTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGGTGGGCATTGGCTTTTTCTGGGTGTGAACGTTTGCAGGGGCGTAAGCCCGCGGCGAGTGCCTCGCTg                                             >  1:3737610/1‑149 (MQ=255)
                                                                                                           cGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCa                                          <  2:3629179/149‑1 (MQ=255)
                                                                                                            ggCTAAGGCTTCCCGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACAGGCGTGGGTTATTTTATCCAACCGATGTTGCTGGGAATTGGCTTTTTCTGGCTGACAACGTTTGCTGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCAt                                         <  1:3198402/149‑1 (MQ=255)
                                                                                                               tatggCTTCCAGGGTTACCGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACACCTTTTGCAGGGGCTAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTg                                      <  1:4602736/146‑1 (MQ=255)
                                                                                                                  ggCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGt                                   >  1:4325447/1‑149 (MQ=255)
                                                                                                                       ccAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATACAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCGGGCATTTGCGTAGAAg                              >  1:2435235/1‑149 (MQ=255)
                                                                                                                            gTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGAc                         >  2:3324533/1‑149 (MQ=255)
                                                                                                                              tACCGGCGTTTCCTGTTCCAGCAGTCGACACGGGTGGGTGATTTTATCCAACCGAGGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGt                       <  2:2616173/149‑1 (MQ=255)
                                                                                                                                cAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCCTTGGCTTTTTCTGGCTGACAACGTTTTCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCAGGCATTTGCGTAGAAGGAGACGttt                     >  2:2918645/1‑149 (MQ=255)
                                                                                                                                   gggTTTCCTGTTCCAGCAGTCGACACGGGTGGGTTTTTTTATCCACCCGTTGTTGCTGGGCTTTGGATTTTTCGGGCTTACAACTTTGCCTGGGGCGAAAGCCCGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTcc                  <  1:575250/147‑1 (MQ=255)
                                                                                                                                     gTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCg                >  2:2787173/1‑149 (MQ=255)
                                                                                                                                      tttCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGc               >  1:5184407/1‑149 (MQ=255)
                                                                                                                                         ccTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCaaa            >  1:5196174/1‑149 (MQ=255)
                                                                                                                                         ccTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCaaa            >  1:1679649/1‑149 (MQ=255)
                                                                                                                                          cTGTCCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGATTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAg           <  1:1553626/149‑1 (MQ=255)
                                                                                                                                            gTTCCAGCAGTCGACAGGGGTGGTTTTTTTTATCCACCCGATGTTGCGGGTCTTTGGCTTTTTCTGGCTGACAACGTGTGCTGGGGCTAACCCCCGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCa         <  1:4423884/149‑1 (MQ=255)
                                                                                                                                            gTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCa         >  1:4662419/1‑149 (MQ=255)
                                                                                                                                               ccAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGt      <  2:3013536/149‑1 (MQ=255)
                                                                                                                                                 agcagTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCt    >  2:1199046/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGG  >  CP009273/2303459‑2303754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑