Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,711,673 A→C 9.2% V72G (GTT→GGT)  yfiF ← putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,711,6730AC 9.2% 913.0 / 9.8 300V72G (GTT→GGT) yfiFputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (208/54);  new base C (2/36);  total (210/90)
Fisher's exact test for biased strand distribution p-value = 1.72e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

TGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGA  >  CP009273/2711526‑2711821
                                                                                                                                                    |                                                                                                                                                      
  gAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑g                                                                                                                                                    >  2:1369297/1‑149 (MQ=255)
   aaaaGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑Gt                                                                                                                                                   <  2:3645019/149‑1 (MQ=255)
       gTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTg‑cgc                                                                                                                                              >  2:4158791/1‑149 (MQ=255)
        tGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGcc                                                                                                                                             <  2:1183535/149‑1 (MQ=255)
          ccTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCAc                                                                                                                                           <  2:1666108/149‑1 (MQ=255)
           cTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACg                                                                                                                                          >  1:4182657/1‑149 (MQ=255)
           cTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACg                                                                                                                                          >  2:1424305/1‑149 (MQ=255)
             gACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGc                                                                                                                                        >  2:1338217/1‑149 (MQ=255)
               cATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGa                                                                                                                                      >  2:3792494/1‑149 (MQ=255)
                   ctttttCGCGGTAGCCGGGGGTTATTCCCGCACGATGACGACGAAACACTTCCGGATCAATAAAACTTTACCCAGTGAT‑GCCACGTTTATCGGCCTTTTCGGGCGTCTAACCCCCGGGCGCGCGGCAAACG‑GTG‑CGCCACGGCGAATCt                                                                                                                                  <  2:2933784/147‑1 (MQ=255)
                    aTTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑TCCACCGTGATCGTCCTTTTCCGGCGTCTCATCACACGGCGCGCGGGACACC‑TTG‑CGCCACGGCGAATCtt                                                                                                                                 >  1:361180/1‑149 (MQ=255)
                    aTTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCtt                                                                                                                                 >  1:1803446/1‑149 (MQ=255)
                         cGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCAc                                                                                                                            >  2:1772804/1‑149 (MQ=255)
                             gTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGc                                                                                                                        >  1:5167439/1‑149 (MQ=255)
                             gTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGc                                                                                                                        >  2:629506/1‑149 (MQ=255)
                              tAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCg                                                                                                                       >  2:3438558/1‑149 (MQ=255)
                               aGACGGGGGTTTCTGCCGCACGCTGACGCCGAAACAGTTCCGGATCAATAAAAATTTTACCACTGAT‑GCCCCCGTTTGCGGCCTTTTCGGGCGTCTCAACACCCGGCGCGCGGGAAACC‑GGG‑CGCCACGGCGAATCTTCCCACTTGCgg                                                                                                                      <  2:4236265/149‑1 (MQ=255)
                               aGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCgg                                                                                                                      >  1:1304356/1‑149 (MQ=255)
                                       gTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACgggg                                                                                                              >  1:1862169/1‑149 (MQ=255)
                                         ttcttcCGCACGCTTCCGACGCAACACTCCCGTATCAATAAAAATTTTCCCAGTGAT‑GCCACCGTGATCGGCCTTGTCCGGCGTCTCATCCCCGGGCGCGGGGGAACCG‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCt                                                                                                            <  2:389235/149‑1 (MQ=255)
                                          tcttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGACTCTTCCCACTTGCGGTCACGGGGCTg                                                                                                           >  1:3112264/1‑149 (MQ=255)
                                            gtcCGCCGGCTGCCGACCCAACCCTGCGGTAACAAAAAAAC‑TTTACCCCTGTTGGCCACCTTCAGGGGCCTTTGCGGGCGTCTCA‑CCCCCGGGGGGGGGGTAACCGGGG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc                                                                                                         <  2:1106088/148‑1 (MQ=255)
                                              ccGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActc                                                                                                       >  2:2293644/1‑149 (MQ=255)
                                                gCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTg                                                                                                     >  1:2958203/1‑149 (MQ=255)
                                                     cTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGt                                                                                                >  1:1872757/1‑149 (MQ=255)
                                                     cTGACGACCAAACACTACCGTATAAATAAAACTTTAACCACTGAG‑GCCACGGTTATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGGGGTAAACC‑GGG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGt                                                                                                <  2:4440975/149‑1 (MQ=255)
                                                      tGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTc                                                                                               >  1:2679666/1‑149 (MQ=255)
                                                        acgacgCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCAt                                                                                             >  2:4684700/1‑149 (MQ=255)
                                                        accacgAAACACGTCCGTAACAATAAAACTTTTCCCACGGAG‑CCCACCGTGTTCGGCCTTTCCGGGCGTCACAACCCCCGGCGCGCGGGAACCC‑GGG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCAt                                                                                             <  2:5038805/146‑1 (MQ=255)
                                                          tccgCACCACTTCCGTATCAATAAAACTTTTCCCACTGAG‑GCCACGGTGATCGGCC‑GTTCGGGCGTCTCATCCCCGGGGGGGCGGTAAACG‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATcgc                                                                                          <  2:2069854/147‑1 (MQ=255)
                                                           acgCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATcgc                                                                                          >  1:2051022/1‑149 (MQ=255)
                                                               aaCACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggc                                                                                      >  1:1026387/1‑149 (MQ=255)
                                                               aaCACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggc                                                                                      >  1:2607541/1‑149 (MQ=255)
                                                                 cacTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcgg                                                                                    >  2:4824742/1‑149 (MQ=255)
                                                                   cTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcgggg                                                                                  >  1:3204959/1‑149 (MQ=255)
                                                                    ttCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcggggc                                                                                 >  1:3987140/1‑149 (MQ=255)
                                                                    ttCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcggggc                                                                                 >  1:555664/1‑149 (MQ=255)
                                                                    ttCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcggggc                                                                                 >  1:555988/1‑149 (MQ=255)
                                                                     tCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcggggcg                                                                                >  1:3778861/1‑149 (MQ=255)
                                                                     tCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcggggcg                                                                                >  1:1167896/1‑149 (MQ=255)
                                                                       cgtaccAATAAAACTTTTCCCCCGGAG‑GCCCCGGTTCGCGCCCTTTCCCGGCGTATCACCCCCCGGCGCGCGGCAACCC‑GGG‑CCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGAc                                                                              <  2:243187/144‑1 (MQ=255)
                                                                       agtataaaTAAAAATTTAACCCCTGCT‑CCCAGGTTTACCGGCCTTTCCGGGCTTCCCAACCCCGGGCG‑GGGGGCAACG‑GGGCCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGAc                                                                              <  1:4671865/143‑1 (MQ=255)
                                                                             aaTAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAc                                                                        >  1:2119126/1‑149 (MQ=255)
                                                                             aaTAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAc                                                                        >  2:3753763/1‑149 (MQ=255)
                                                                              aTAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAcc                                                                       >  2:4763052/1‑149 (MQ=255)
                                                                               tAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCg                                                                      >  2:4270433/1‑149 (MQ=255)
                                                                               tAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCg                                                                      >  2:3257745/1‑149 (MQ=255)
                                                                                     ttttACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCa                                                                >  1:1192821/1‑149 (MQ=255)
                                                                                     ttttACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCa                                                                >  1:2822640/1‑149 (MQ=255)
                                                                                     ttttACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCa                                                                >  1:2562948/1‑149 (MQ=255)
                                                                                     ttttACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTGTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCa                                                                >  1:3374899/1‑149 (MQ=255)
                                                                                     ttttACCACGGAG‑GCCACCTTGATGGGCCTTTCCCGGCGTCTCAACCCCCGGCGCGGGGGAAACC‑GTG‑CCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCa                                                                <  2:304529/149‑1 (MQ=255)
                                                                                           cACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAt                                                          >  1:1964662/1‑149 (MQ=255)
                                                                                            aCTGCT‑GCCACGTTGTCCGGCATTTCCGGGCGTCACACCCCCGGGGGCG‑GGGCAACC‑GGGCCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAtt                                                         <  1:1604423/149‑1 (MQ=255)
                                                                                             cctgagcccACCGTGATCGGCATGTGCCGGCGTCTCAACACCGGGCGCGCGGTAAACG‑GTG‑CCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAtt                                                         <  1:4721183/142‑1 (MQ=255)
                                                                                             cTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAttt                                                        >  2:3993285/1‑149 (MQ=255)
                                                                                               gAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTc                                                      >  2:2451807/1‑149 (MQ=255)
                                                                                                   gCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTg                                                   >  2:786297/1‑149 (MQ=255)
                                                                                                    ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTgg                                                  >  2:531267/1‑149 (MQ=255)
                                                                                                     cACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGTGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGa                                                 >  1:4910219/1‑149 (MQ=255)
                                                                                                        cGTGCTCGGCCTTTCCGGGCTTCACCCCCCCCGGCGCGGGGCAACCC‑GGC‑CCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgc                                              <  1:1759738/149‑1 (MQ=255)
                                                                                                        cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgc                                              >  1:3919121/1‑149 (MQ=255)
                                                                                                        cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgc                                              >  1:3079734/1‑149 (MQ=255)
                                                                                                         gttaTCGGCTTTTGCCGGGGTCACACCCCCCGGCGCGCGGGAACCG‑GGG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcc                                             <  2:205691/146‑1 (MQ=255)
                                                                                                         gTGAGCGGCCTTTGCGGGCGTCTCATCCCCCGGCGCGCGGTAAACG‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcc                                             <  2:205555/149‑1 (MQ=255)
                                                                                                          ttaTCGGCCTTTGCGGGCTTCTCAACACCGGGCGGGGGGGAACCG‑GGG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgccc                                            <  1:3792494/147‑1 (MQ=255)
                                                                                                          tgcgcgcccTTTTCGGGCGACCCACCCCCGGGCGGGGGGACACCC‑GGG‑CCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgccc                                            <  1:1420285/142‑1 (MQ=255)
                                                                                                          tGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGCCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgccc                                            >  1:350573/1‑149 (MQ=255)
                                                                                                            aTCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccgc                                          >  2:61303/1‑149 (MQ=255)
                                                                                                            aTCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccgc                                          >  2:1919614/1‑149 (MQ=255)
                                                                                                             tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccgcc                                         >  2:2197322/1‑149 (MQ=255)
                                                                                                             tCGGCCTTTGCCGGCGACACAACCCCGGGCGCGCGGGAACCC‑GGG‑CCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccgcc                                         <  2:180226/149‑1 (MQ=255)
                                                                                                             tCGGCCGGTGCCGGCGTCTAACCACCCGGCGCGCGGTAAACC‑GTC‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccgcc                                         <  2:2773684/149‑1 (MQ=255)
                                                                                                               ggCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGcccc                                       >  2:2107493/1‑149 (MQ=255)
                                                                                                                 ccTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCt                                     >  2:4106025/1‑149 (MQ=255)
                                                                                                                  cTTTGCCGGGGGCCCATCCCCGGGGGGGCGGGAAACG‑GGG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCtt                                    <  2:1506975/149‑1 (MQ=255)
                                                                                                                   ttttCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCttt                                   >  1:4070677/1‑149 (MQ=255)
                                                                                                                   ttttCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCttt                                   >  2:369548/1‑149 (MQ=255)
                                                                                                                   ttttCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCttt                                   >  2:4349236/1‑149 (MQ=255)
                                                                                                                    ttgccGGCGTCTAACCACCCGGCGCGCGGCAACCC‑GGG‑CCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTc                                  <  2:565638/146‑1 (MQ=255)
                                                                                                                      tcgggCTTCTCAACCCCCGGCGCGCGGTAAACG‑GGC‑CCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTccc                                <  1:1550424/146‑1 (MQ=255)
                                                                                                                          gCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTa                            >  2:4989921/1‑149 (MQ=255)
                                                                                                                          gCGTCACACCCCCGGGCGCGGGGGAACCG‑GGC‑CCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTa                            <  1:89663/149‑1 (MQ=255)
                                                                                                                           cttctcATCACCCGGCGCGGGGTAACCC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAc                           <  2:1551762/147‑1 (MQ=255)
                                                                                                                             tctcATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcgc                         >  2:4573777/1‑149 (MQ=255)
                                                                                                                             tctcATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcgc                         >  2:26214/1‑149 (MQ=255)
                                                                                                                              ctcATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcgcg                        >  2:3497954/1‑149 (MQ=255)
                                                                                                                              ctcATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcgcg                        >  1:5171607/1‑149 (MQ=255)
                                                                                                                                catcaCCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCggg                      >  1:2749726/1‑149 (MQ=255)
                                                                                                                                aatcaCCGGGCGGGCGTTAAACG‑GTG‑CCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCggg                      <  1:3828417/148‑1 (MQ=255)
                                                                                                                                 aacaCCCGGCGCGCGGTAAACG‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGt                     <  2:5168049/147‑1 (MQ=255)
                                                                                                                                  tcaCCCGGCGCGGGGTAAACG‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGtt                    <  2:3778865/149‑1 (MQ=255)
                                                                                                                                  tcaCCCGGCGCGCGGGAACCC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGtt                    >  1:2585493/1‑149 (MQ=255)
                                                                                                                                  tcaCCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGtt                    <  2:1167896/149‑1 (MQ=255)
                                                                                                                                      cgggCGCGCGGCAAACG‑GGG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTgg                <  2:1872757/147‑1 (MQ=255)
                                                                                                                                      ccGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTgg                >  2:879066/1‑149 (MQ=255)
                                                                                                                                        ggCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGt              >  1:4560683/1‑149 (MQ=255)
                                                                                                                                               gggAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTGTCCCGGTACGCGGGTTGTGGGTACGTTTa       <  2:4928675/149‑1 (MQ=255)
                                                                                                                                               gggAAACC‑GGG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTa       <  2:3505268/149‑1 (MQ=255)
                                                                                                                                                 gAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATc     >  2:2674614/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGAT‑GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGA  >  CP009273/2711526‑2711821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑