Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,835,291 T→G 8.9% C315G (TGC→GGC)  ascB → cryptic 6‑phospho‑beta‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,835,2910TG 8.9% 790.0 / 5.0 282C315G (TGC→GGC) ascBcryptic 6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand):  ref base T (103/141);  new base G (32/2);  total (139/143)
Fisher's exact test for biased strand distribution p-value = 2.87e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

TATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTA  >  CP009273/2835143‑2835439
                                                                                                                                                    |                                                                                                                                                    
tATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTGTCTTTCAGCTATTAAGCCTCGCGCt                                                                                                                                                      >  2:1352522/1‑149 (MQ=255)
  tCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTCTCGTTCAGCTATTACGCCACGCGCGgc                                                                                                                                                    >  2:3613231/1‑149 (MQ=255)
  tCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTCAGCTATTACGCCTCGCGCTgc                                                                                                                                                    >  2:1621278/1‑149 (MQ=255)
      tgtgCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCt                                                                                                                                                <  1:1765129/149‑1 (MQ=255)
       gtgCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTc                                                                                                                                               <  2:2301167/149‑1 (MQ=255)
        tgCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTcg                                                                                                                                              <  1:5169621/149‑1 (MQ=255)
         gCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCCGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTcgg                                                                                                                                             <  2:1582000/149‑1 (MQ=255)
          cAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTATCTTTAAGCTATTACCCCTCCCGCGGCGcccccgc                                                                                                                                            >  1:3221768/1‑144 (MQ=255)
           aGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCAcggcg                                                                                                                                           >  2:4711883/1‑149 (MQ=255)
            ggCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTcggcgg                                                                                                                                          >  2:3357363/1‑149 (MQ=255)
            ggCGCGGGGCACGTATCCGGCTTACACTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTAAGCTATTAAGCCACGCGCTGCGCCGcggcgg                                                                                                                                          >  1:4985483/1‑149 (MQ=255)
               gcgGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGgaga                                                                                                                                       <  2:3752035/149‑1 (MQ=255)
                cgGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGAt                                                                                                                                      <  1:4953478/149‑1 (MQ=255)
                 ggggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATg                                                                                                                                     <  2:155897/149‑1 (MQ=255)
                  gggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGa                                                                                                                                    <  1:1246350/149‑1 (MQ=255)
                  gggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTGTATTTGAGAGAGTTAGCCGCGCGCGGCGCCGCGGCGGCGGTGa                                                                                                                                    >  1:3070788/1‑149 (MQ=255)
                    gCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACGATAAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAAc                                                                                                                                  <  1:4650249/149‑1 (MQ=255)
                    gCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTATCTTTAAGCTATTAAGCCACGCGCGGCGCCACGGCGGAGATTaaa                                                                                                                                  >  2:902277/1‑148 (MQ=255)
                      aCGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGc                                                                                                                                <  1:136725/149‑1 (MQ=255)
                      aCGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGc                                                                                                                                <  1:2726642/149‑1 (MQ=255)
                               gCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTGGGTTTGATCAATTGAGCCGCGCGGGGCGCCGCGGGGGAGGTGTACCCCAaaaacaa                                                                                                                       >  1:4833973/1‑148 (MQ=255)
                                cTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAAcagc                                                                                                                      <  1:1096417/149‑1 (MQ=255)
                                  tACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAAcagcag                                                                                                                    <  2:2537780/149‑1 (MQ=255)
                                  tACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAAcagcag                                                                                                                    <  1:4608351/149‑1 (MQ=255)
                                  tACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAAcagcag                                                                                                                    <  1:4230643/149‑1 (MQ=255)
                                  tACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAAcagcag                                                                                                                    <  2:2034739/149‑1 (MQ=255)
                                   aCTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGt                                                                                                                   <  2:3934318/149‑1 (MQ=255)
                                    ctctGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTATATTTCATCTATTACGCCACGCGCTGCGCCACGGCGGAGATGAACGCCAACAAAATCAGTg                                                                                                                  >  2:1867659/1‑149 (MQ=255)
                                     tctGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTgc                                                                                                                 <  1:2550552/149‑1 (MQ=255)
                                      ctGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCGATTACGCCACGCGCGGCGCCGCGGCGGAGCTGAAAGCCAAAAAAAGCAGTgcg                                                                                                                >  1:1644982/1‑149 (MQ=255)
                                        gCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTgcggc                                                                                                              <  1:4039607/149‑1 (MQ=255)
                                        gCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTgcggc                                                                                                              <  1:4980638/149‑1 (MQ=255)
                                          ccGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTCACCGAGTACGCCACCCGCCGCGCCCCGGCGGCGCTGAACGCCCCCCACACCCGAgcggcgc                                                                                                            >  1:4377905/1‑148 (MQ=255)
                                           cgcgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGaa                                                                                                           <  2:4151162/149‑1 (MQ=255)
                                           cgcgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTCTATTTAAGCTATTAAGCCACCCGCAGCGCCACGGCGGAGATTAACGCCCACAACAACAGTGCGGCGaa                                                                                                           >  1:1860221/1‑149 (MQ=255)
                                            gcgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAAc                                                                                                          <  2:1982860/149‑1 (MQ=255)
                                            gcgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAAc                                                                                                          <  2:4592207/149‑1 (MQ=255)
                                            gcgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTATCTTTCAGCGATTAAGCCTCGCGCTGCGCCTCGGCGGAGAAGAACGCCAACAACAGCACTGCGGCGCAc                                                                                                          >  2:4436375/1‑149 (MQ=255)
                                             cgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTCAGCTATTAAGCCACCCGCGGCGCCGCCGCGGAGATGAAAGCCCAAAACAGCAGTGCGGCGAAcc                                                                                                         >  1:5162151/1‑148 (MQ=255)
                                               tATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACgtg                                                                                                       <  2:4839058/149‑1 (MQ=255)
                                                aTTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTAAGCTAATAAGCCTCGCGCTGCGCCACGGCGGAGATGAAAGCCAACAACAGCAGTGCGGCGAACgtgg                                                                                                      >  2:3181566/1‑149 (MQ=255)
                                                       gAAAAAGGGGTAACCATCAACAACGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATc                                                                                               <  2:1010156/149‑1 (MQ=255)
                                                       gAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATc                                                                                               <  2:3864635/149‑1 (MQ=255)
                                                          aaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTCAGCTATTACCCCACGCGCTGCGCCGCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGCACGGGGTGTAATCGcg                                                                                            >  2:4528504/1‑148 (MQ=255)
                                                                gTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTaa                                                                                      <  1:3618047/149‑1 (MQ=255)
                                                                gTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGGGGTGAAATCGCTGCGTaa                                                                                      >  2:2912767/1‑149 (MQ=255)
                                                                   aCCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTCTCTTTCAGCGAATACGCCGCGCGCTGCGCCACGGCGGAGATTAACGCCAACAACAGCACTGCGGCGAACGTGGTGAAATCGCTGCGTAAtac                                                                                   >  2:4033507/1‑147 (MQ=255)
                                                                     cATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGt                                                                                 <  1:2317904/149‑1 (MQ=255)
                                                                       tCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTGAGCGCTTAAGCCGCGCGCTGCGCCTCGGCGGAGCTGAACGCCAACAACAGCAGTGCGGCGAACGTGGGGAAATCGCTGCGTAATACGTAt                                                                               >  2:1307415/1‑149 (MQ=255)
                                                                         aacaaAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCGATTACGCCACGCGCTGCGCCGCGGCGGTGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCt                                                                             >  2:3246018/1‑149 (MQ=255)
                                                                          acaaAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCGGCGCCTCGGCGGAGATGACCGCCAACCACAGCAGTGCGGCGCACGTGGTGAAATCGCTGCGTAATCCGTATcga                                                                            >  2:4646126/1‑147 (MQ=255)
                                                                            aaaGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACa                                                                          <  2:3592160/149‑1 (MQ=255)
                                                                             aaGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCAATTACCCCGCGCGCGGCGCCTCGGCGGAGATGAACGCCAACAACAGCACTGCGGCGAACGTGGTGAAAACGCCGCGGACTCCGTATCTACac                                                                         >  2:4719816/1‑148 (MQ=255)
                                                                                 aCCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGa                                                                     <  2:1972979/149‑1 (MQ=255)
                                                                                 aCCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGa                                                                     <  2:2483691/149‑1 (MQ=255)
                                                                                    gggCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCg                                                                  <  2:4163026/149‑1 (MQ=255)
                                                                                       cGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGAct                                                               <  1:3472474/149‑1 (MQ=255)
                                                                                            atgaAATCCTGAAAAACACCGTCGATTTTGTCTATTTCAGCTATTAAGCCACGCGCTGCGCCGCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATACGTATCTACAGGTGAGCGActggggc                                                          >  2:4076633/1‑149 (MQ=255)
                                                                                               aaaTCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActggggctgg                                                       <  2:2124811/149‑1 (MQ=255)
                                                                                                aaTCCTGAAAAACACCGTCGAGTTTGTGTCTTTCCGCGATTACGCCGCGCGCTGCGCCTCGGCGGGGGTGAACGCCCACAACACCAGTGCGGCGCAAGTGGTGTAAACGCGGCGGAATCCGGATAAACAGGGGAGCGCcgggggctggg                                                      >  1:2539030/1‑149 (MQ=255)
                                                                                                aaTCCTGAAAAACACCGTCGAGTTTGTCTATTTCAGCTAATAAGCCACCCGCTGCCCCTCCGCGGAGATTAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAAAAGCTGCGGAAAACGTATATACACGTGAGCCCcaggggcgggg                                                      >  1:4689271/1‑149 (MQ=255)
                                                                                                 aTCCTGAAAAACACCGTCGATTTTGTCTCTTTCCGCTAGTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActggggctgggg                                                     <  2:3659312/149‑1 (MQ=255)
                                                                                                 aTCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActggggctgggg                                                     <  1:2999174/149‑1 (MQ=255)
                                                                                                 aTCCTGAAAAACACCGTCGATTTTGTATCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAAAAACAGCAGTGCGGCGCACGTTGTGAAATCGCTGCGTAATACGTATCTACAGGTGAGCGActgggggtgggg                                                     >  2:5108096/1‑149 (MQ=255)
                                                                                                  tCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCCCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGa                                                    <  1:3391453/149‑1 (MQ=255)
                                                                                                    cTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAAt                                                  <  2:1212378/149‑1 (MQ=255)
                                                                                                    cTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAAt                                                  <  2:550772/149‑1 (MQ=255)
                                                                                                      gAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCGGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAAACGCTGCGTAATCCGTATAAACAGGTGAGCGACTGGGGCTGGGGAAttt                                                >  2:4283985/1‑148 (MQ=255)
                                                                                                          aaCACCGTCGAGTTTTTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCACGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGGATCTACAGGTGAGCGACAGGGGCTGGGGAATTGATcc                                            >  2:5189643/1‑149 (MQ=255)
                                                                                                              ccGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTc                                        <  2:2320791/149‑1 (MQ=255)
                                                                                                               cGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATACACTCg                                       >  2:1726096/1‑149 (MQ=255)
                                                                                                               cGTCGATTTTGTCTCTTTAAGCTATTACGCCTCGCGCGGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATACACACg                                       >  1:3174927/1‑149 (MQ=255)
                                                                                                                  cGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGACTCGGCGGAGATGAACGCAAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGGTTATTCCGTATATAAAGTTGAGCGACTGGGGATGGGGAATTGATCCACTCGGTc                                    <  1:784769/149‑1 (MQ=255)
                                                                                                                    aTTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTg                                  <  2:4974451/149‑1 (MQ=255)
                                                                                                                      tttGTCTCTTTCAGCTATTCCGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCg                                <  1:1848521/149‑1 (MQ=255)
                                                                                                                      tttGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAAGGTGGTGAAATCGCTGCGTAATCCGTATCTACAGTTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCg                                <  1:4460660/149‑1 (MQ=255)
                                                                                                                       ttGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGt                               <  2:1702705/149‑1 (MQ=255)
                                                                                                                        tGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTa                              >  2:2618110/1‑149 (MQ=255)
                                                                                                                        tGTCTCGTGCCGCTATTCCGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACCCCCGCAGTGCGGCGAACGTGGGGAAAGCGCTGCGTAATCCGGATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTa                              <  2:2773299/149‑1 (MQ=255)
                                                                                                                          tctcTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATc                            >  2:3085086/1‑149 (MQ=255)
                                                                                                                          tctcTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATc                            >  2:4327246/1‑149 (MQ=255)
                                                                                                                          tctcTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATc                            >  2:1932114/1‑149 (MQ=255)
                                                                                                                                  aGCTATTACGCCTCGCGCTGCGCCTCGGCGTATATGAACGCCAACAACAGCAGTGCGGCGAACGTGTTGAAATCTATGCGTAATCCGTATCTACAGTTTAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGaa                    <  2:3330320/149‑1 (MQ=255)
                                                                                                                                  aGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGaa                    >  1:910030/1‑149 (MQ=255)
                                                                                                                                    cTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAata                  <  1:335570/149‑1 (MQ=255)
                                                                                                                                     tATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAatat                 <  2:3427543/149‑1 (MQ=255)
                                                                                                                                     tATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAatat                 >  1:2459085/1‑149 (MQ=255)
                                                                                                                                      aTTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatg                >  1:1122221/1‑149 (MQ=255)
                                                                                                                                       ttACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatga               <  1:4100465/149‑1 (MQ=255)
                                                                                                                                        tACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatgat              >  2:3048361/1‑149 (MQ=255)
                                                                                                                                         aCGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatgatg             >  1:441940/1‑149 (MQ=255)
                                                                                                                                         aCGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatgatg             >  2:4915860/1‑149 (MQ=255)
                                                                                                                                          cGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGt            >  2:1688311/1‑149 (MQ=255)
                                                                                                                                          cGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGt            >  2:257660/1‑149 (MQ=255)
                                                                                                                                           gCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTa           >  1:2121326/1‑149 (MQ=255)
                                                                                                                                           gCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTa           >  1:2033031/1‑149 (MQ=255)
                                                                                                                                             cTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACg         <  2:329315/149‑1 (MQ=255)
                                                                                                                                              tCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGa        >  1:1625344/1‑149 (MQ=255)
                                                                                                                                                gcgcTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATc      >  1:4518653/1‑149 (MQ=255)
                                                                                                                                                  gcTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGt    <  2:2399443/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTA  >  CP009273/2835143‑2835439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑