Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 435,336 G→T 94.1% Q100K (CAG→AAG)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273435,3360GT94.1% 968.0 / 43.9 307Q100K (CAG→AAG) dxs1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
Reads supporting (aligned to +/- strand):  ref base G (9/9);  new base T (147/142);  total (156/151)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

Only 100 of 307 total aligned reads displayed.

CACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGAG  >  CP009273/435189‑435481
                                                                                                                                                   |                                                                                                                                                 
cacaGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGAc                                                                                                                                                 >  2:2471126/1‑150 (MQ=255)
  caGACGGTGCGGCGATTTTTTCCTTCTTTTTAGGCAGCAACCCCAAAACCAAATCCGGCACTGATGGAGGTTGATGAATTCCCCACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACgg                                                                                                                                               >  1:385282/1‑150 (MQ=255)
   aGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGCTGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTGTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGa                                                                                                                                              >  2:745181/1‑150 (MQ=255)
   aGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGGCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGa                                                                                                                                              >  2:2144640/1‑150 (MQ=255)
    gACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGAt                                                                                                                                             >  1:1030985/1‑150 (MQ=255)
     aCGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATg                                                                                                                                            >  2:3576122/1‑150 (MQ=255)
      cGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATgg                                                                                                                                           <  1:2689508/150‑1 (MQ=255)
        gTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTg                                                                                                                                         <  1:821149/150‑1 (MQ=255)
          gcggcgATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGcc                                                                                                                                       <  1:3415686/150‑1 (MQ=255)
          gcggcgATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGcc                                                                                                                                       <  1:2024055/150‑1 (MQ=255)
               gATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGAttt                                                                                                                                  >  1:1818944/1‑150 (MQ=255)
               gATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATACCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGAttt                                                                                                                                  >  1:957270/1‑150 (MQ=255)
                   tttGCCTTCTTTTTCGGCATCAACCGCAATACCAATTCCGTCACTGATGGAGGTTGATGAATGCCCGTCGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGTTTCAGACCGCCTTTCTTACGGATGGTTCCGATTTTGTc                                                                                                                              <  1:4134894/150‑1 (MQ=255)
                      gCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTcgcg                                                                                                                           <  2:1387945/150‑1 (MQ=255)
                       ccTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCgcgg                                                                                                                          >  1:2971062/1‑150 (MQ=255)
                           cttTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTc                                                                                                                      >  1:4349112/1‑150 (MQ=255)
                            tttttCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTcc                                                                                                                     <  2:3211370/150‑1 (MQ=255)
                            tttttCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTcc                                                                                                                     >  1:3832476/1‑150 (MQ=255)
                            tttttCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTcc                                                                                                                     <  2:3038325/150‑1 (MQ=255)
                              tttCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCgg                                                                                                                   <  2:3419946/150‑1 (MQ=255)
                                tCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTc                                                                                                                 <  1:2515377/150‑1 (MQ=255)
                                 cGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCa                                                                                                                <  1:1090920/150‑1 (MQ=255)
                                  ggCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGACCGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCaa                                                                                                               >  2:3854219/1‑150 (MQ=255)
                                  ggCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCaa                                                                                                               >  2:3370069/1‑150 (MQ=255)
                                  ggCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCaa                                                                                                               >  2:1668499/1‑150 (MQ=255)
                                     agcaACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAAt                                                                                                            <  2:2728529/150‑1 (MQ=255)
                                      gcaACCGCAAGACCACTTGCGGGACTGATGGAGGTTTATGAATGCACGTCGCTTATTTCTTCATCTTCTCTTTCGCCGCGACTAGGGAAGGTGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAAtt                                                                                                           <  1:753179/150‑1 (MQ=255)
                                       caACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAAttt                                                                                                          >  2:14829/1‑150 (MQ=255)
                                         aCCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTa                                                                                                        >  1:1682183/1‑150 (MQ=255)
                                          ccGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTAt                                                                                                       <  2:896120/150‑1 (MQ=255)
                                              aaTACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCgg                                                                                                   <  1:264423/150‑1 (MQ=255)
                                              aaTACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCgg                                                                                                   >  1:4282889/1‑150 (MQ=255)
                                                 aCCAAGTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATa                                                                                                <  2:2846249/150‑1 (MQ=255)
                                                  ccAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCGTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATaa                                                                                               <  1:2721993/150‑1 (MQ=255)
                                                         cGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGAt                                                                                        <  1:3281518/150‑1 (MQ=255)
                                                         cGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGAt                                                                                        >  2:1122243/1‑150 (MQ=255)
                                                           gCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGc                                                                                      <  1:2901001/150‑1 (MQ=255)
                                                              cTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGcccc                                                                                   >  2:3948101/1‑150 (MQ=255)
                                                              cTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGcccc                                                                                   <  1:313951/150‑1 (MQ=255)
                                                              cTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGcccc                                                                                   >  1:361286/1‑150 (MQ=255)
                                                                 aTGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCcaca                                                                                <  1:3510682/150‑1 (MQ=255)
                                                                  tGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACAt                                                                               >  1:4076002/1‑150 (MQ=255)
                                                                   ggAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATc                                                                              >  2:772627/1‑150 (MQ=255)
                                                                     aGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATccc                                                                            <  2:897664/150‑1 (MQ=255)
                                                                     aGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATccc                                                                            <  2:3772548/150‑1 (MQ=255)
                                                                       gTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCaa                                                                          <  1:518475/150‑1 (MQ=255)
                                                                        ttGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCaaa                                                                         >  1:2087661/1‑150 (MQ=255)
                                                                             gaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAAt                                                                    <  2:332531/150‑1 (MQ=255)
                                                                             gaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGAGGCCCCACATCCCAAATCAAt                                                                    >  2:2572504/1‑150 (MQ=255)
                                                                                  cccGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTc                                                               <  2:1818944/150‑1 (MQ=255)
                                                                                    cGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCaa                                                             >  2:1321410/1‑150 (MQ=255)
                                                                                    cGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCaa                                                             >  1:2985581/1‑150 (MQ=255)
                                                                                    cGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCaa                                                             <  1:1135702/150‑1 (MQ=255)
                                                                                    cGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCaa                                                             >  2:1687344/1‑150 (MQ=255)
                                                                                    cGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCaa                                                             >  2:1888010/1‑150 (MQ=255)
                                                                                    cGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCaa                                                             <  1:15666/150‑1 (MQ=255)
                                                                                     gACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCACATCAATTGGTCaaa                                                            >  2:1893301/1‑150 (MQ=255)
                                                                                      aCGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAAc                                                           <  2:2494547/150‑1 (MQ=255)
                                                                                      aCGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAAc                                                           >  2:2536632/1‑150 (MQ=255)
                                                                                        gCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACgg                                                         <  2:3922987/150‑1 (MQ=255)
                                                                                        gCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACgg                                                         >  1:583199/1‑150 (MQ=255)
                                                                                        gCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACgg                                                         >  1:2763063/1‑150 (MQ=255)
                                                                                         cTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACggg                                                        >  2:314250/1‑150 (MQ=255)
                                                                                                 gTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTa                                                <  1:3306289/150‑1 (MQ=255)
                                                                                                    atatTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATCAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGAc                                             >  2:2057148/1‑150 (MQ=255)
                                                                                                    atatTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGAc                                             >  2:1545919/1‑150 (MQ=255)
                                                                                                    atatTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGAc                                             >  2:4295784/1‑150 (MQ=255)
                                                                                                    atatTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGAc                                             <  1:684766/150‑1 (MQ=255)
                                                                                                     tatTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACa                                            >  2:1876867/1‑150 (MQ=255)
                                                                                                     tatTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACa                                            <  1:933342/150‑1 (MQ=255)
                                                                                                       ttCGCTTTCGCCGCGCCACGGGTACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATa                                          <  1:3665874/150‑1 (MQ=255)
                                                                                                        tCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACCTAg                                         >  2:2268898/1‑150 (MQ=255)
                                                                                                          gCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTg                                       <  2:967778/150‑1 (MQ=255)
                                                                                                          gCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTg                                       <  2:2401098/150‑1 (MQ=255)
                                                                                                             ttCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAg                                    <  1:2212007/150‑1 (MQ=255)
                                                                                                                  cgcgCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCa                               >  2:2307109/1‑150 (MQ=255)
                                                                                                                  cgcgCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCa                               >  2:435356/1‑150 (MQ=255)
                                                                                                                   gcgcCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCAc                              <  1:1264066/150‑1 (MQ=255)
                                                                                                                     gcCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACgg                            >  2:3733930/1‑150 (MQ=255)
                                                                                                                       cACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTc                          >  1:3650076/1‑150 (MQ=255)
                                                                                                                       cACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTc                          <  2:3633882/150‑1 (MQ=255)
                                                                                                                        aCGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCa                         <  1:3040696/150‑1 (MQ=255)
                                                                                                                         cGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAACCGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAg                        <  2:712086/150‑1 (MQ=255)
                                                                                                                         cGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAg                        <  2:3640326/150‑1 (MQ=255)
                                                                                                                         cGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAg                        <  2:2621189/150‑1 (MQ=255)
                                                                                                                         cGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAg                        >  1:2871640/1‑150 (MQ=255)
                                                                                                                         cGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAg                        <  2:3826891/150‑1 (MQ=255)
                                                                                                                          gggAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGt                       <  1:3134135/150‑1 (MQ=255)
                                                                                                                          gggAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGt                       <  2:2915448/150‑1 (MQ=255)
                                                                                                                          gggAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGt                       >  1:2552794/1‑150 (MQ=255)
                                                                                                                             aaCGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCg                    >  1:4218374/1‑150 (MQ=255)
                                                                                                                                gggTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGAcc                 <  2:1250998/150‑1 (MQ=255)
                                                                                                                                 ggTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCg                >  1:2335201/1‑150 (MQ=255)
                                                                                                                                   tGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTg              <  2:1242607/150‑1 (MQ=255)
                                                                                                                                   tGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTg              >  1:3586156/1‑150 (MQ=255)
                                                                                                                                     cAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGcc            >  2:3545801/1‑150 (MQ=255)
                                                                                                                                     cAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGcc            <  1:1982360/150‑1 (MQ=255)
                                                                                                                                     cAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTCATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGcc            <  1:144760/150‑1 (MQ=255)
                                                                                                                                       gACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCa          <  2:3099235/150‑1 (MQ=255)
                                                                                                                                             cTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCgg    >  2:101780/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGAG  >  CP009273/435189‑435481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑