Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 441,811 A→C 20.7% I104M (ATT→ATG)  yajR ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273441,8110AC20.7% 579.2 / 195.9 294I104M (ATT→ATG) yajRputative transporter
Reads supporting (aligned to +/- strand):  ref base A (118/115);  new base C (27/34);  total (145/149)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.86e-01

Only 100 of 312 total aligned reads displayed.

AGCACCATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACC  >  CP009273/441662‑441960
                                                                                                                                                     |                                                                                                                                                     
aGCACCATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGaata                                                                                                                                                       >  1:3777227/1‑150 (MQ=255)
    ccATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTc                                                                                                                                                   <  2:642514/150‑1 (MQ=255)
     cATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTcc                                                                                                                                                  >  2:2511046/1‑150 (MQ=255)
      aTCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTccc                                                                                                                                                 >  1:1535196/1‑150 (MQ=255)
          cAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGa                                                                                                                                             >  1:3508373/1‑150 (MQ=255)
            aTGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATg                                                                                                                                           <  1:1340743/150‑1 (MQ=255)
            aTGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATg                                                                                                                                           >  2:3679237/1‑150 (MQ=255)
             tGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATgg                                                                                                                                          >  2:1039722/1‑150 (MQ=255)
                   aGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCgg                                                                                                                                    >  1:1462459/1‑150 (MQ=255)
                    ggTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGa                                                                                                                                   >  2:3476431/1‑150 (MQ=255)
                     gTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGaa                                                                                                                                  >  2:3538868/1‑150 (MQ=255)
                       aaTGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTAGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTTCACCAGAGCCTTGTAGCGCCCGGCCCAGAATAATACCCCAGATGGAGTCGGAAAg                                                                                                                                >  2:509768/1‑150 (MQ=255)
                         tGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAgcg                                                                                                                              >  2:2826960/1‑150 (MQ=255)
                            cAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAg                                                                                                                           <  2:2985338/150‑1 (MQ=255)
                              aaGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCg                                                                                                                         >  1:984041/1‑150 (MQ=255)
                               aGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGa                                                                                                                        >  2:246117/1‑150 (MQ=255)
                                gCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGAt                                                                                                                       <  1:3675373/150‑1 (MQ=255)
                                 cTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATa                                                                                                                      <  1:1010379/150‑1 (MQ=255)
                                   cACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAAc                                                                                                                    <  1:2374280/150‑1 (MQ=255)
                                     cGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGc                                                                                                                  >  2:746240/1‑150 (MQ=255)
                                      gCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCt                                                                                                                 <  2:3111056/150‑1 (MQ=255)
                                      gCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCt                                                                                                                 <  2:1498386/150‑1 (MQ=255)
                                      gCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCt                                                                                                                 >  2:397684/1‑150 (MQ=255)
                                        cGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTTCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTAc                                                                                                               >  2:2122040/1‑150 (MQ=255)
                                         gATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTAcc                                                                                                              >  1:2549317/1‑150 (MQ=255)
                                                gCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCa                                                                                                       >  2:1467793/1‑150 (MQ=255)
                                                gCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCa                                                                                                       >  2:1183174/1‑150 (MQ=255)
                                                gCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCa                                                                                                       >  2:3334558/1‑150 (MQ=255)
                                                  cATCGCTTTGGTGCTGTTGTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCTGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCaaa                                                                                                     <  2:2623808/150‑1 (MQ=255)
                                                  cATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCaaa                                                                                                     >  1:2508696/1‑150 (MQ=255)
                                                    tCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAaca                                                                                                   >  2:1272321/1‑150 (MQ=255)
                                                      gCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACAcc                                                                                                 <  1:1052805/150‑1 (MQ=255)
                                                         ttGGTGCGGTTTTGTTCGCGCGTGAGATCGGATAGCAGCGCCTTAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGcc                                                                                              <  1:3349279/150‑1 (MQ=255)
                                                           ggTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAg                                                                                            >  1:2137074/1‑150 (MQ=255)
                                                               cGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCa                                                                                        >  1:2737183/1‑150 (MQ=255)
                                                                ggTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCAc                                                                                       >  2:2188691/1‑150 (MQ=255)
                                                                ggTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCAc                                                                                       >  1:2675496/1‑150 (MQ=255)
                                                                ggTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCAc                                                                                       >  2:953360/1‑150 (MQ=255)
                                                                 gTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCAcc                                                                                      >  1:2806143/1‑150 (MQ=255)
                                                                 gTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTACAGAGCCTTGTAGCGCCCGGCCAAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGAGATAACGCTACCGGCGGCAAACACCGCCAGCCCAcc                                                                                      <  1:1402501/150‑1 (MQ=255)
                                                                   tttGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGa                                                                                    >  1:2842008/1‑150 (MQ=255)
                                                                   tttGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGa                                                                                    <  2:1655278/150‑1 (MQ=255)
                                                                      gTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACaa                                                                                 >  1:4259322/1‑150 (MQ=255)
                                                                      gTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACaa                                                                                 >  2:1947799/1‑150 (MQ=255)
                                                                       gtCGCGCGTGAGAGCGGACAGCCGCGCCATAACGGCGGCGGCAAGTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAAGTCCCCAGATGGAGTCGGAAAGCGCAGCGATCACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAAt                                                                                <  2:3571530/149‑1 (MQ=255)
                                                                        tCGCGCGTGAGATGGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAAtt                                                                               <  1:3408441/150‑1 (MQ=255)
                                                                        tCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAAtt                                                                               >  1:3061821/1‑150 (MQ=255)
                                                                          gcgcgTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTaa                                                                             <  1:2703543/150‑1 (MQ=255)
                                                                           cgcgTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAAt                                                                            >  1:3801527/1‑150 (MQ=255)
                                                                           cgcgTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAAt                                                                            <  1:300022/150‑1 (MQ=255)
                                                                               tGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGtt                                                                        <  2:4319530/150‑1 (MQ=255)
                                                                                     cGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGAc                                                                  >  2:3101750/1‑150 (MQ=255)
                                                                                        aaaGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCaa                                                               <  1:1668316/150‑1 (MQ=255)
                                                                                        aaaGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCaa                                                               >  1:3189002/1‑150 (MQ=255)
                                                                                         aaGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAAt                                                              <  2:2093693/150‑1 (MQ=255)
                                                                                          agcagcGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATg                                                             <  2:3768221/150‑1 (MQ=255)
                                                                                          agcagcGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATg                                                             >  2:1831941/1‑150 (MQ=255)
                                                                                           gcagcGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGc                                                            <  1:355729/150‑1 (MQ=255)
                                                                                           gcagcGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAGTGc                                                            <  1:3298200/150‑1 (MQ=255)
                                                                                            cagcGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTCCCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCg                                                           >  2:1294500/1‑150 (MQ=255)
                                                                                            cagcGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCg                                                           >  1:3981486/1‑150 (MQ=255)
                                                                                            cagcGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCg                                                           >  2:4283875/1‑150 (MQ=255)
                                                                                               cgcCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTc                                                        <  1:1968514/150‑1 (MQ=255)
                                                                                                 ccATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCgg                                                      >  2:1615876/1‑150 (MQ=255)
                                                                                                   aTAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGaa                                                    >  2:3260569/1‑150 (MQ=255)
                                                                                                    tAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGaaa                                                   >  1:1172739/1‑150 (MQ=255)
                                                                                                     aaCGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAg                                                  <  2:1761863/150‑1 (MQ=255)
                                                                                                     aaCGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAg                                                  <  2:2354927/150‑1 (MQ=255)
                                                                                                      aCGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGc                                                 >  1:1818883/1‑150 (MQ=255)
                                                                                                       cggcggcggCAATTGCTACAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACTATTAATGGTTTGCGACCAATGCGGTCGGAAAGCa                                                <  2:3830817/150‑1 (MQ=255)
                                                                                                          cggcggcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGc                                             <  2:2746745/150‑1 (MQ=255)
                                                                                                          cggcggcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGc                                             <  2:3777424/150‑1 (MQ=255)
                                                                                                           ggcggcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGcc                                            >  2:3276608/1‑150 (MQ=255)
                                                                                                           ggcggcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGcc                                            >  1:895685/1‑150 (MQ=255)
                                                                                                               gcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAAc                                        <  1:3719649/150‑1 (MQ=255)
                                                                                                               gcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAAGTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAAc                                        <  1:4117650/150‑1 (MQ=255)
                                                                                                                  aTTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGa                                     >  1:3380396/1‑150 (MQ=255)
                                                                                                                      cTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCt                                 >  1:852562/1‑150 (MQ=255)
                                                                                                                        ccAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGa                               >  2:2145522/1‑150 (MQ=255)
                                                                                                                          agagCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGaaa                             <  2:2231319/150‑1 (MQ=255)
                                                                                                                          agagCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGaaa                             <  2:57628/150‑1 (MQ=255)
                                                                                                                           gagCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGaaaa                            <  1:4323361/150‑1 (MQ=255)
                                                                                                                           gagCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGaaaa                            >  1:1842077/1‑150 (MQ=255)
                                                                                                                             gCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAAc                          >  2:1568594/1‑150 (MQ=255)
                                                                                                                                 tGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGcc                      <  2:2914804/150‑1 (MQ=255)
                                                                                                                                   tAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTg                    >  2:526164/1‑150 (MQ=255)
                                                                                                                                    aGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGa                   >  1:4311624/1‑150 (MQ=255)
                                                                                                                                        cccGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCa               <  2:548808/150‑1 (MQ=255)
                                                                                                                                        cccGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCa               <  1:4270701/150‑1 (MQ=255)
                                                                                                                                         ccGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAg              >  2:1801184/1‑150 (MQ=255)
                                                                                                                                          cGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGa             >  2:4174466/1‑150 (MQ=255)
                                                                                                                                           ggCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGAc            >  1:1510871/1‑150 (MQ=255)
                                                                                                                                           ggCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGAc            >  1:513232/1‑150 (MQ=255)
                                                                                                                                             cccAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCa          >  1:1916266/1‑150 (MQ=255)
                                                                                                                                             cccAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCa          >  1:766643/1‑150 (MQ=255)
                                                                                                                                                 gAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATaaa      >  1:1323724/1‑150 (MQ=255)
                                                                                                                                                 gAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATaaa      <  2:843735/150‑1 (MQ=255)
                                                                                                                                                  aataatTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAAt     >  2:352428/1‑150 (MQ=255)
                                                                                                                                                   ataatTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATa    >  1:657993/1‑150 (MQ=255)
                                                                                                                                                     aatTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATAcc  <  2:1342422/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCACCATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACC  >  CP009273/441662‑441960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑