Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,290 G→A 100% intergenic (+45/‑299) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,2900GA94.7% 792.4 / 24.0 229intergenic (+45/‑299)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (7/5);  new base A (95/121);  total (102/127)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 230 total aligned reads displayed.

TTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCC  >  CP009273/450142‑450435
                                                                                                                                                    |                                                                                                                                                    
ttCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGa                                                                                                                                                     >  2:806393/1‑150 (MQ=255)
ttCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGa                                                                                                                                                     >  2:806251/1‑150 (MQ=255)
  cATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                                   >  1:1490876/1‑150 (MQ=255)
  cATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                                   <  1:2726231/150‑1 (MQ=255)
    tGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtttt                                                                                                                                                 >  2:2340957/1‑150 (MQ=255)
        cTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGa                                                                                                                                             <  1:3220592/150‑1 (MQ=255)
        cTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGa                                                                                                                                             >  1:2997756/1‑150 (MQ=255)
           gCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaa                                                                                                                                          >  2:3859667/1‑150 (MQ=255)
            ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                         <  2:3799955/150‑1 (MQ=255)
            ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                         >  2:1720949/1‑150 (MQ=255)
             tctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAc                                                                                                                                        <  1:1152220/150‑1 (MQ=255)
              ctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACg                                                                                                                                       <  1:2952820/150‑1 (MQ=255)
                 cATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGcc                                                                                                                                    >  2:908548/1‑150 (MQ=255)
                    aCTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGc                                                                                                                                 >  1:1798374/1‑150 (MQ=255)
                    aCTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGc                                                                                                                                 >  1:3167472/1‑150 (MQ=255)
                    aCTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGc                                                                                                                                 >  1:4109637/1‑150 (MQ=255)
                      ttACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAAGGCCTGCgg                                                                                                                               >  1:880283/1‑150 (MQ=255)
                      ttACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCgg                                                                                                                               <  2:1084655/150‑1 (MQ=255)
                        acacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGc                                                                                                                             >  2:3641853/1‑150 (MQ=255)
                         cacTATTAAGGAGTGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGcc                                                                                                                            <  1:1284279/150‑1 (MQ=255)
                          acTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCg                                                                                                                           >  2:3849816/1‑150 (MQ=255)
                          acTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCg                                                                                                                           <  2:619921/150‑1 (MQ=255)
                              ttAACGTGTGGGCAGGGTTGCAGGACACCGACTTTGCCACTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTACCAGTAGGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtttt                                                                                                                       <  1:3267898/150‑1 (MQ=255)
                                aaGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtgttgt                                                                                                                     >  2:1311132/1‑150 (MQ=255)
                                aaGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtgttgg                                                                                                                     >  1:1023215/1‑149 (MQ=255)
                                  ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGGCgtgttgttt                                                                                                                   >  1:3337638/1‑150 (MQ=255)
                                           aGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGG‑At                                                                                                         >  1:3978768/1‑150 (MQ=255)
                                           aGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑At                                                                                                         <  2:2697745/150‑1 (MQ=255)
                                           aGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑At                                                                                                         >  2:1033140/1‑150 (MQ=255)
                                            gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑Att                                                                                                        <  2:3037583/150‑1 (MQ=255)
                                            gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑Att                                                                                                        <  2:3234742/150‑1 (MQ=255)
                                            gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑Att                                                                                                        <  1:2188410/150‑1 (MQ=255)
                                             ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTTTGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑Attt                                                                                                       <  1:485342/150‑1 (MQ=255)
                                             ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTTG‑Attt                                                                                                       >  2:2843414/1‑150 (MQ=255)
                                              gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑Atttt                                                                                                      <  2:3556325/150‑1 (MQ=255)
                                                       taaggTCTTTGCCTCTCCTCCCTGTCTTGGAGCATGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCttttt                                                                                             <  2:1378495/146‑1 (MQ=255)
                                                       taaggTCTTTGCCTCTCCTCCCGGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCttttt                                                                                             <  2:3325169/146‑1 (MQ=255)
                                                       cacCGTCTTTGCCACTCCTCCCTGTCGTGGAGCAGTAAGCATCGCTTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATTTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCttttt                                                                                             <  1:1916792/150‑1 (MQ=255)
                                                        acCGTCTGTGCCTCTCCTCCCTGGCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTg                                                                                            <  1:2977773/150‑1 (MQ=255)
                                                          cGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGTAAGCATCGCGTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGcc                                                                                          <  1:1077378/150‑1 (MQ=255)
                                                           gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTGGTTTTTTGGGGTTTTTGcc                                                                                          >  1:874171/1‑150 (MQ=255)
                                                               ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTGG‑GTTTTTGGCTTTTTTGCCAGC‑a                                                                                    >  1:2050345/1‑150 (MQ=255)
                                                                tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑At                                                                                   >  2:1671113/1‑150 (MQ=255)
                                                                tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCGGG‑ATTTTGGGCTTTTTTCCCaccca                                                                                    >  1:987707/1‑146 (MQ=255)
                                                                  ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGGTTTTTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTc                                                                                 >  2:1932235/1‑150 (MQ=255)
                                                                   ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCTGG‑ATTTTGCGCGTTTTGCCCAGC‑ATTCa                                                                                >  2:3393069/1‑150 (MQ=255)
                                                                    tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAg                                                                               <  2:1828596/150‑1 (MQ=255)
                                                                       cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTGG‑GTTTTTGGGTTTTTGACCCGC‑AATCAGACg                                                                            >  1:1680031/1‑150 (MQ=255)
                                                                        ctccCTGTCGTGGAACAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGa                                                                           >  2:2505267/1‑150 (MQ=255)
                                                                        ctccCTGTCGTGGAACAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGa                                                                           >  2:330789/1‑150 (MQ=255)
                                                                           ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGaaaa                                                                        <  1:2537843/150‑1 (MQ=255)
                                                                             tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAt‑t                                                                     <  1:2305069/150‑1 (MQ=255)
                                                                             tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAt‑t                                                                     <  1:3633304/150‑1 (MQ=255)
                                                                              gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑Tg                                                                    <  1:970510/150‑1 (MQ=255)
                                                                              gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCCGC‑AATCAGACGAAAAT‑Tg                                                                    >  2:301382/1‑150 (MQ=255)
                                                                              gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGAAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑Tg                                                                    <  1:3753774/150‑1 (MQ=255)
                                                                                cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGG‑ATTTTTCGCTTTTTGGCCAGC‑ATTCAGACGCAAAT‑TGcc                                                                  >  2:3380976/1‑150 (MQ=255)
                                                                                cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑CTTCAGACGAAAAG‑TGcc                                                                  >  2:3243937/1‑150 (MQ=255)
                                                                                cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGcc                                                                  <  1:1639000/150‑1 (MQ=255)
                                                                                cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGcc                                                                  <  1:792650/150‑1 (MQ=255)
                                                                                cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCGGG‑TTTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGcc                                                                  >  2:3605868/1‑150 (MQ=255)
                                                                                cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTTG‑GTTTTTGGCTTTTTTACCACC‑ATTGAGACGaaaaaagcc                                                                   >  2:1811960/1‑145 (MQ=255)
                                                                                cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑CTTCAGACGCAAAA‑TGcc                                                                  >  2:2294636/1‑150 (MQ=255)
                                                                                 gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGccc                                                                 <  1:2168751/150‑1 (MQ=255)
                                                                                  tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCg                                                                <  1:3047883/150‑1 (MQ=255)
                                                                                  tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTGGTTTTTTGGGTTTTTTGCCC‑GC‑ATTAAGGCGGAAAT‑Tggcgg                                                                >  1:3899098/1‑146 (MQ=255)
                                                                                   ggAGCAGGAAGCATCGCGTAAAAACGCATGTGCAACTGGCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCgg                                                               <  1:2581949/150‑1 (MQ=255)
                                                                                    gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGG‑ATTTTGGGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCggg                                                              >  2:214311/1‑150 (MQ=255)
                                                                                     aGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGa                                                             <  1:1798840/150‑1 (MQ=255)
                                                                                        aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAAtt                                                          <  2:1757292/150‑1 (MQ=255)
                                                                                         ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCTGG‑ATTTTGCGCTTTTTTCCCAGC‑ATTCAGTCGAAAAT‑TGCCCGGGAATtg                                                         >  2:1743444/1‑150 (MQ=255)
                                                                                            aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGa                                                      <  1:1012570/150‑1 (MQ=255)
                                                                                             gCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCGGCGGGCGGTTTTTTTTTGTGGGG‑TTTTTGGGGGTTTTTGCCACC‑AATCAGGCGATAAT‑TGGCCGGGTATagttta                                                     >  1:583392/1‑147 (MQ=255)
                                                                                                  gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAAt                                                <  1:3129329/150‑1 (MQ=255)
                                                                                                  gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAAt                                                <  1:3201745/150‑1 (MQ=255)
                                                                                                   cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTTCGCTTTTTTCCCAGC‑ATTAAGACGAAAAT‑TTGCCGGGAATTGTGAAAAAATa                                               >  2:4086974/1‑150 (MQ=255)
                                                                                                   cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATa                                               <  1:4321408/150‑1 (MQ=255)
                                                                                                    gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATAc                                              <  2:638574/150‑1 (MQ=255)
                                                                                                         caCGCATGTGCAACTGGCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGAc                                         <  2:2205867/149‑1 (MQ=255)
                                                                                                         aaCGCATTTGCAACTGTCGGCGCTTTTCCATTATTTTTCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGAc                                         <  2:2206292/150‑1 (MQ=255)
                                                                                                            gCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTGTTTTTTGTGG‑GTTTTTGGGTTTTTTGGCACC‑CAACAGACGAAAATATGCCCGGG‑ATTGTGTAAAAAAAAGCGCCacc                                      >  1:1201925/1‑148 (MQ=255)
                                                                                                              aTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTTCCCAGC‑AATAAGACCAAAAA‑TGCCCCGGGATTGTGAAAAAAAACGCCACAccgc                                    >  2:2622929/1‑150 (MQ=255)
                                                                                                                    aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa                              <  2:1233497/150‑1 (MQ=255)
                                                                                                                      cTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAc                            <  2:1658398/150‑1 (MQ=255)
                                                                                                                           ggCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTGTATGG‑ATTTTTGGCTTTTTGCCCACC‑ATTCAGACCAAAAA‑TGACCGGGAATTTTGTAAAAATAAGCGACAGCGCGCAAAAACCCTtc                       >  2:1267688/1‑150 (MQ=255)
                                                                                                                           ggCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtc                       <  2:2794246/150‑1 (MQ=255)
                                                                                                                            gcgcTTTGACAGTATGTTTCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtct                      <  1:4246642/150‑1 (MQ=255)
                                                                                                                              gcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTTG‑ATTTTTCGCTTTTTGCCCACC‑AATCAGAACAAAAT‑TTCCCGGGAATTGTGAAAAAAAAAGCGACAGCGCGCAATAAcccatctag                    >  2:2258779/1‑143 (MQ=255)
                                                                                                                               cTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                   <  2:1503294/150‑1 (MQ=255)
                                                                                                                                ttttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTTCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATAAGCGACAGCGCGCAATAACCGTTCTCGAc                  >  2:3939938/1‑150 (MQ=255)
                                                                                                                                ttttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTTTGTGG‑ATTTTTCGCTTTTTTACCAGCAATACGCACAAAAAT‑TCCCCGGGAA‑TGTTAAAAAAAAAGCGAACCCGCCCAAAAACCGATCTCGAc                  >  2:1116796/1‑150 (MQ=255)
                                                                                                                                 tttCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAAGTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACt                 <  1:106665/150‑1 (MQ=255)
                                                                                                                                    ccAGTATGTTGCTAAAAATGTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTAGGG‑ATTTTGCGCTTTTTTCCCAGC‑ATTCAGACGAAAAG‑TGCCCGGGAATTTTGTAAAAATACGCGGAAGCGCGCACAAACCGGTATCGACTCAt              >  2:3531495/1‑150 (MQ=255)
                                                                                                                                     ctgtttGTTGATAAAATTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATa             <  1:3784832/145‑1 (MQ=255)
                                                                                                                                     cAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATa             <  2:1198702/150‑1 (MQ=255)
                                                                                                                                     cAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATa             <  2:207284/150‑1 (MQ=255)
                                                                                                                                       gTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaa           <  2:2627069/150‑1 (MQ=255)
                                                                                                                                           gTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTg       <  2:2606231/150‑1 (MQ=255)
                                                                                                                                            tggCTAAAATTTTTATTAAAAACGGCCGGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAATT‑TGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGa      <  1:3288990/148‑1 (MQ=255)
                                                                                                                                               cTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTGTTTTTTCTGG‑ATTTTTCGCGTTTTTCCCAGC‑AATAAGACGAAAAT‑TGGCCGGGCATTGTGAAAAAATAAGCGACAGCGCGCAATAAACGTTCTCGACTCAAAAAAGTGATGc   >  2:3700635/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCC  >  CP009273/450142‑450435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑