Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 594,191 C→T 7.2% R8C (CGC→TGC)  cusA → copper/silver efflux system, membrane component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273594,1910CT 7.2% 882.7 / 38.5 250R8C (CGC→TGC) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base C (124/108);  new base T (9/9);  total (133/117)
Fisher's exact test for biased strand distribution p-value = 8.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 268 total aligned reads displayed.

GGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAGCTATCCCGGTCAGGCA  >  CP009273/594042‑594340
                                                                                                                                                     |                                                                                                                                                     
ggtctggCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATtcgtc                                                                                                                                                       <  1:4115563/150‑1 (MQ=255)
 gtctggCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATtcgtcg                                                                                                                                                      <  1:960079/150‑1 (MQ=255)
   ctggCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTTGCt                                                                                                                                                    <  2:306663/150‑1 (MQ=255)
       cGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGt                                                                                                                                                <  2:1290551/150‑1 (MQ=255)
        ggAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTg                                                                                                                                               <  2:2259858/150‑1 (MQ=255)
         gAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTgg                                                                                                                                              <  2:2949115/150‑1 (MQ=255)
         gAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTgg                                                                                                                                              <  2:2948577/150‑1 (MQ=255)
           aGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCg                                                                                                                                            >  1:81343/1‑150 (MQ=255)
            ggTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGa                                                                                                                                           >  2:2337597/1‑150 (MQ=255)
             gTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGaa                                                                                                                                          >  2:77756/1‑150 (MQ=255)
              tGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAAc                                                                                                                                         >  2:2138837/1‑150 (MQ=255)
              tGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAAc                                                                                                                                         >  2:4329165/1‑150 (MQ=255)
               gAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAccc                                                                                                                                        >  1:3005518/1‑150 (MQ=255)
               gAAAAGGTGGTTTCCAGCGGCCTGGTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAAcc                                                                                                                                        <  2:3458174/150‑1 (MQ=255)
                aaaaGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCg                                                                                                                                       >  1:1797159/1‑150 (MQ=255)
                       ggtTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTTGCTCGGTGGCGAACCGTTTTTTg                                                                                                                                >  1:2041566/1‑150 (MQ=255)
                       ggtTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTg                                                                                                                                >  1:293044/1‑150 (MQ=255)
                           tCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCCTGCGCATTGAGGGAATAACCACTGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGc                                                                                                                            <  2:3836650/150‑1 (MQ=255)
                           tCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGc                                                                                                                            <  2:2867087/150‑1 (MQ=255)
                            ccAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCt                                                                                                                           <  2:200228/150‑1 (MQ=255)
                             cAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTg                                                                                                                          <  1:4272085/150‑1 (MQ=255)
                             cAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTg                                                                                                                          <  2:2544673/150‑1 (MQ=255)
                               gCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGAt                                                                                                                        <  1:888242/150‑1 (MQ=255)
                                   ccTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTTGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGgc                                                                                                                    >  1:4346541/1‑150 (MQ=255)
                                     tGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGgcgc                                                                                                                  >  1:630968/1‑150 (MQ=255)
                                       ttCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCgt                                                                                                                >  1:3185155/1‑150 (MQ=255)
                                            gattgattCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGttt                                                                                                           <  2:476811/150‑1 (MQ=255)
                                             attgattCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGACCCGTTTTTTGGTGCTGATGGGCGCGTTGTTTc                                                                                                          >  2:3392846/1‑150 (MQ=255)
                                             attgattCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTc                                                                                                          >  2:4357289/1‑150 (MQ=255)
                                                gattCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGa                                                                                                       >  2:3754406/1‑150 (MQ=255)
                                                 attCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAg                                                                                                      <  1:2726333/150‑1 (MQ=255)
                                                      gAAGCCAATATTTCTGTCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCTCATTTAGGTAATAAACAATGATTGAATGGATTATTATTCGCTCGGTGGCGAACCGTTTTTTTGTGATGATTGGATAGTTGTTTCTGAGCATCt                                                                                                 >  2:902219/1‑150 (MQ=255)
                                                       aaGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTg                                                                                                <  2:1977880/150‑1 (MQ=255)
                                                         gCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTggg                                                                                              <  2:743673/150‑1 (MQ=255)
                                                            aaTATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGGTGGGCGCGTTGTTTCTGAGCATCTGGGGCa                                                                                           >  2:4068897/1‑150 (MQ=255)
                                                              tatTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCAcc                                                                                         >  2:3637432/1‑150 (MQ=255)
                                                              tatTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCAcc                                                                                         >  2:1169642/1‑150 (MQ=255)
                                                               atTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCt                                                                                        >  2:4255287/1‑150 (MQ=255)
                                                                 ttCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTTGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTgg                                                                                      <  2:3941309/150‑1 (MQ=255)
                                                                    tGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGAcc                                                                                   <  1:3926740/150‑1 (MQ=255)
                                                                          aCTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGTGCATCTGGGGCACCTGGACCATCAtt                                                                             >  1:371047/1‑150 (MQ=255)
                                                                          aCTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCAtt                                                                             >  2:3660316/1‑150 (MQ=255)
                                                                          aCTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCAtt                                                                             >  1:2440566/1‑150 (MQ=255)
                                                                           ctcgAGCGGATGCGCTCAGAACGTGCTACCCCTGCGCATTGCGGGAAGAACCAATGCTTGACTGGATGATTCGTCGCGCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTa                                                                            <  1:3422095/147‑1 (MQ=255)
                                                                           cTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTTGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTa                                                                            <  2:1034110/150‑1 (MQ=255)
                                                                           cTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTa                                                                            <  1:1279450/150‑1 (MQ=255)
                                                                             ggAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAAt                                                                          >  2:470366/1‑150 (MQ=255)
                                                                                gCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACg                                                                       <  1:2794907/150‑1 (MQ=255)
                                                                                 cGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTGTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGc                                                                      >  1:4153944/1‑150 (MQ=255)
                                                                                     tGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGt                                                                  <  2:376532/150‑1 (MQ=255)
                                                                                          tctGAAAGTGCTACCCATGCGCATGGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATg                                                             <  2:3285480/150‑1 (MQ=255)
                                                                                             gAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATgcgc                                                          >  1:591324/1‑150 (MQ=255)
                                                                                              aaaGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCt                                                         >  2:3673477/1‑150 (MQ=255)
                                                                                                 gTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGcc                                                      <  2:4157595/150‑1 (MQ=255)
                                                                                                  tGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCg                                                     >  1:1025517/1‑150 (MQ=255)
                                                                                                     tACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTTCTGATTGGCGCGTTGTTTATTATCATCTGGGGCACCTGGACCATCATTAATAAGCCAGTGGATGCGCTGCCGGAt                                                  >  1:2547614/1‑150 (MQ=255)
                                                                                                      aCCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGAtc                                                 >  1:3244866/1‑150 (MQ=255)
                                                                                                      aCCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGAtc                                                 >  1:937596/1‑150 (MQ=255)
                                                                                                        ccATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGAtctc                                               <  2:1330308/150‑1 (MQ=255)
                                                                                                           tGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTcc                                            >  2:688119/1‑150 (MQ=255)
                                                                                                           tGCGCATTGAGGGAATAACCAATGATGGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTcc                                            <  2:2308330/150‑1 (MQ=255)
                                                                                                              gcATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGAt                                         <  1:688854/150‑1 (MQ=255)
                                                                                                              gcATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGAt                                         <  2:1070129/150‑1 (MQ=255)
                                                                                                                 ttGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGAtgtg                                      <  2:1954497/150‑1 (MQ=255)
                                                                                                                 ttGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGAtgtg                                      <  2:3310335/150‑1 (MQ=255)
                                                                                                                     gggAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAgg                                  >  1:837976/1‑150 (MQ=255)
                                                                                                                      ggAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGt                                 <  2:2354461/150‑1 (MQ=255)
                                                                                                                       gAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGtt                                <  2:1808002/150‑1 (MQ=255)
                                                                                                                        aaTAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGtta                               >  2:591385/1‑150 (MQ=255)
                                                                                                                        aaTAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGtta                               >  1:1711493/1‑150 (MQ=255)
                                                                                                                         aTAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGttat                              >  2:4292770/1‑150 (MQ=255)
                                                                                                                         aTAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGttat                              <  2:2401772/150‑1 (MQ=255)
                                                                                                                          tAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGttatt                             <  1:3027810/150‑1 (MQ=255)
                                                                                                                           aaCCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGttatta                            <  1:1149819/150‑1 (MQ=255)
                                                                                                                           aaCCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGttatta                            <  1:4023710/150‑1 (MQ=255)
                                                                                                                           aaCCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGttatta                            <  2:1638991/150‑1 (MQ=255)
                                                                                                                           aaCCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGttatta                            <  1:787148/150‑1 (MQ=255)
                                                                                                                             ccAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGttattatt                          <  1:2608357/150‑1 (MQ=255)
                                                                                                                             ccAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGttattatt                          >  1:2196904/1‑150 (MQ=255)
                                                                                                                             ccAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGttattatt                          <  1:432291/150‑1 (MQ=255)
                                                                                                                              cAATGATTGACTGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATCCGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGttattatta                         <  1:2279469/150‑1 (MQ=255)
                                                                                                                                 tGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTaaaa                      >  2:614745/1‑150 (MQ=255)
                                                                                                                                    ttGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCa                   <  2:1847573/150‑1 (MQ=255)
                                                                                                                                     tGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGCTGTGCAGGGTAGTATTAAAACCAg                  >  1:475458/1‑150 (MQ=255)
                                                                                                                                     tGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAg                  >  2:4284734/1‑150 (MQ=255)
                                                                                                                                     tGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAg                  >  2:783188/1‑150 (MQ=255)
                                                                                                                                       aaTGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAGCt                >  2:83432/1‑150 (MQ=255)
                                                                                                                                       aaTGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAGCt                <  1:2337597/150‑1 (MQ=255)
                                                                                                                                       aaTGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAGCt                <  2:3645208/150‑1 (MQ=255)
                                                                                                                                        aTGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAGCTa               >  2:1903203/1‑150 (MQ=255)
                                                                                                                                           gATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTTTTGGGCGCGTTGTTTATGAGCATCTGGGGCACCAGGACCATCATTAATACGCCACTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAGCTATcc            >  1:368786/1‑150 (MQ=255)
                                                                                                                                           gATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAGCTATcc            >  2:3494570/1‑150 (MQ=255)
                                                                                                                                              tattCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAGCTATCCCgg         >  2:2500427/1‑150 (MQ=255)
                                                                                                                                               attCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAGCTATCCCGGt        <  2:3169133/150‑1 (MQ=255)
                                                                                                                                               attCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAGCTATCCCGGt        >  1:3889199/1‑150 (MQ=255)
                                                                                                                                                ttCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAGCTATCCCGGTc       >  2:2767374/1‑150 (MQ=255)
                                                                                                                                                   gtcgCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAGCTATCCCGGTCAgg    >  1:2102562/1‑150 (MQ=255)
                                                                                                                                                   gtcgCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAGCTATCCCGGTCAgg    >  2:2279266/1‑150 (MQ=255)
                                                                                                                                                    tcgCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAGCTATCCCGGTCAGGc   >  1:1643904/1‑150 (MQ=255)
                                                                                                                                                    tcgCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCT‑GGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAGCTATCCCGGTCAGGca  <  1:1525004/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAATGGATTATTCGTCGCTCGGTGGCGAACCGTTTTTTGGTGCTGATGGGCGCGTTGTTTCTGAGCATCTGGGGCACCTGGACCATCATTAATACGCCAGTGGATGCGCTGCCGGATCTCTCCGATGTGCAGGTTATTATTAAAACCAGCTATCCCGGTCAGGCA  >  CP009273/594042‑594340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑