Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 650,141 G→T 14.4% V428V (GTC→GTA)  dcuC ← anaerobic C4‑dicarboxylate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273650,1410GT14.4% 837.1 / 81.7 288V428V (GTC→GTA) dcuCanaerobic C4‑dicarboxylate transport
Reads supporting (aligned to +/- strand):  ref base G (125/118);  new base T (18/27);  total (143/145)
Fisher's exact test for biased strand distribution p-value = 1.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

TGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTCGGGAT  >  CP009273/649992‑650290
                                                                                                                                                     |                                                                                                                                                     
tGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACg                                                                                                                                                       >  1:2880955/1‑150 (MQ=255)
tGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACg                                                                                                                                                       <  1:827684/150‑1 (MQ=255)
    tGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACtt                                                                                                                                                   <  2:3869192/150‑1 (MQ=255)
     gACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTc                                                                                                                                                  <  1:3104651/150‑1 (MQ=255)
      aCATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCa                                                                                                                                                 >  2:2529674/1‑150 (MQ=255)
        aTGATAAAGCCGGAGGAAACTTGCCTCCGGAATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACACGCACCGGTACCGAGGTGCGTTTTACGACTTCaaa                                                                                                                                               <  1:1088009/150‑1 (MQ=255)
            tAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACACTCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGc                                                                                                                                           >  2:2136202/1‑150 (MQ=255)
              aaGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCga                                                                                                                                         >  1:617633/1‑150 (MQ=255)
                   ggaggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCt                                                                                                                                    >  2:4112952/1‑150 (MQ=255)
                    gaggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCtt                                                                                                                                   >  1:370817/1‑150 (MQ=255)
                         aaCTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCa                                                                                                                              >  2:2574250/1‑150 (MQ=255)
                          aCTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTACCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCAt                                                                                                                             <  1:1930807/150‑1 (MQ=255)
                           cTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATc                                                                                                                            >  1:969767/1‑150 (MQ=255)
                            ttGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCTTTTTACGACTTCAAACGGCGAGATCTTCGCCATcc                                                                                                                           <  2:1889265/150‑1 (MQ=255)
                            ttGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATcc                                                                                                                           >  1:3865461/1‑150 (MQ=255)
                             tGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTCCAAACGGCGAGATCTTCGCCATccc                                                                                                                          <  2:2439665/150‑1 (MQ=255)
                               ccTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCgg                                                                                                                        >  1:2325007/1‑150 (MQ=255)
                                   cGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAAc                                                                                                                    >  2:1256941/1‑150 (MQ=255)
                                     gCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCg                                                                                                                  >  1:61695/1‑150 (MQ=255)
                                      cATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGc                                                                                                                 >  1:756382/1‑150 (MQ=255)
                                      cATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGc                                                                                                                 >  1:2926447/1‑150 (MQ=255)
                                       aTTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCa                                                                                                                >  2:3064667/1‑150 (MQ=255)
                                         tACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAAc                                                                                                              >  1:1033865/1‑150 (MQ=255)
                                          aCTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACg                                                                                                             >  1:3661265/1‑150 (MQ=255)
                                          aCTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACg                                                                                                             <  1:3451819/150‑1 (MQ=255)
                                          aCTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACg                                                                                                             >  1:85563/1‑150 (MQ=255)
                                          aCTATTTACTTGCCTGTGACCGCTGCGGCCGGTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACg                                                                                                             <  1:2785853/150‑1 (MQ=255)
                                              tttACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTa                                                                                                         >  1:2726110/1‑150 (MQ=255)
                                                  cTTGCCTGTGCCCGCTGCGGCCGTTCGTGGCACCATCAGCTCTGTAGCAACTATAACAATCACCAACCCACCAAGCACGGGTACCGAGGTGCTTTTTACTACTCCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGcc                                                                                                     <  1:4122988/150‑1 (MQ=255)
                                                         gtgACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACg                                                                                              <  2:4316097/150‑1 (MQ=255)
                                                         gtgACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGATTTACGACTTCAAACGGCGAGATCTTCGCCATCCCCGCAACCGCAACGACTACGCCAGCAACg                                                                                              >  1:2558708/1‑150 (MQ=255)
                                                            aCCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGc                                                                                           <  1:3246723/150‑1 (MQ=255)
                                                            aCCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGc                                                                                           <  2:3240571/150‑1 (MQ=255)
                                                             ccGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACACGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCg                                                                                          <  2:4039568/150‑1 (MQ=255)
                                                              cGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGa                                                                                         >  2:2667978/1‑150 (MQ=255)
                                                                ctgctgCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGaaa                                                                                       >  2:1569182/1‑150 (MQ=255)
                                                                 tgctgcCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAg                                                                                      >  1:3089188/1‑150 (MQ=255)
                                                                  gctgcCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAgg                                                                                     <  1:3907648/150‑1 (MQ=255)
                                                                    ggcCGTTCCTGGCACCATCAGCTCTGTAGCAACGAAAACAACCACCAAACCAACACCCACCGGCACCGAGGTGCTTTTTACTACTACAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGt                                                                                   <  1:3350053/149‑1 (MQ=255)
                                                                     gcCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTa                                                                                  <  2:3829400/150‑1 (MQ=255)
                                                                       cGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACg                                                                                <  2:1224089/150‑1 (MQ=255)
                                                                       cGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACg                                                                                <  2:3418150/150‑1 (MQ=255)
                                                                            cTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCaa                                                                           >  2:2189252/1‑150 (MQ=255)
                                                                             tGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAg                                                                          <  2:2738190/150‑1 (MQ=255)
                                                                             tGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAg                                                                          <  2:662888/150‑1 (MQ=255)
                                                                                cACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACACGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGtt                                                                       <  1:2374379/150‑1 (MQ=255)
                                                                                cACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGtt                                                                       <  2:3201555/150‑1 (MQ=255)
                                                                                cACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGtt                                                                       >  2:3997033/1‑150 (MQ=255)
                                                                                cACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGtt                                                                       >  2:2275451/1‑150 (MQ=255)
                                                                                    aTCAGCTCTGTAGCAACGATAACAATCACCACACCAACCAGCACCGGTACCGCGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGAc                                                                   <  1:2458181/150‑1 (MQ=255)
                                                                                    aTCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGAc                                                                   >  2:77872/1‑150 (MQ=255)
                                                                                    aTCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGAc                                                                   <  2:3472065/150‑1 (MQ=255)
                                                                                    aTCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGAc                                                                   <  2:4350926/150‑1 (MQ=255)
                                                                                       aGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGcc                                                                >  2:1120665/1‑150 (MQ=255)
                                                                                       aGCTCTGTAGCAACGATAACAAACACCAAACCCCCACGCACCGGTACCGCGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGcc                                                                <  1:441770/150‑1 (MQ=255)
                                                                                             gTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTgcagc                                                          >  1:1979954/1‑150 (MQ=255)
                                                                                               aGCAACGATAACAATCACCAACCCAACACGCACCGGTACCGAGGTGCGTTTTACTACTACAACCGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCAt                                                        <  1:2598922/150‑1 (MQ=255)
                                                                                               aGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGGGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCAt                                                        <  1:680747/150‑1 (MQ=255)
                                                                                                  aaCGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCgg                                                     >  2:3585874/1‑150 (MQ=255)
                                                                                                  aaCGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCgg                                                     >  2:2406694/1‑150 (MQ=255)
                                                                                                   aCGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCggg                                                    <  2:2854700/150‑1 (MQ=255)
                                                                                                    cGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGa                                                   >  1:1174441/1‑150 (MQ=255)
                                                                                                      aTAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATa                                                 <  2:1514938/150‑1 (MQ=255)
                                                                                                      aTAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATa                                                 >  1:1759834/1‑150 (MQ=255)
                                                                                                      aTAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATa                                                 <  2:2203349/150‑1 (MQ=255)
                                                                                                       tAACAATCACCAAACCAACACGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAg                                                <  1:4217147/150‑1 (MQ=255)
                                                                                                         aCAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTc                                              >  1:3236659/1‑150 (MQ=255)
                                                                                                          cAATCACCAACCCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCa                                             <  2:1842278/150‑1 (MQ=255)
                                                                                                           aaTCACCACACCACCACGCCCCGGTACCGCGGGGCGTTTTACTACTTCACACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCaa                                            <  1:538085/150‑1 (MQ=255)
                                                                                                             tCACCAAACCACCCCGCACCGGTACCGAGGTGCTTTTTACTACTACAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAAt                                          <  1:3411442/150‑1 (MQ=255)
                                                                                                             tCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAAt                                          >  1:2196408/1‑150 (MQ=255)
                                                                                                               accaaaccaaCACGCACCGGTACCGAGGTGCGTTTTACTACTTCACACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATAc                                        <  1:128078/150‑1 (MQ=255)
                                                                                                                ccaaaccaaCACGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACg                                       <  2:377757/150‑1 (MQ=255)
                                                                                                                ccaaaccaaCAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAACCGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACg                                       <  2:1672687/150‑1 (MQ=255)
                                                                                                                ccaaaccaaCAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACg                                       <  2:1387297/150‑1 (MQ=255)
                                                                                                                ccaaaccaaCAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACg                                       <  2:1950840/150‑1 (MQ=255)
                                                                                                                  aaaccaaCAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGcc                                     <  1:2759218/150‑1 (MQ=255)
                                                                                                                  aaaccaaCAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGcc                                     >  2:3680310/1‑150 (MQ=255)
                                                                                                                   aaccaaCAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCg                                    >  1:634821/1‑150 (MQ=255)
                                                                                                                   aaccaaCAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCg                                    >  1:1863504/1‑150 (MQ=255)
                                                                                                                   aaccaaCAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCg                                    >  1:3330429/1‑150 (MQ=255)
                                                                                                                    accaaCAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCgg                                   >  1:3800903/1‑150 (MQ=255)
                                                                                                                     ccaccaCGCACCGGTCCCGCGGGGCGTTTTACTCCTTCCAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTACAGCATCGGGATAGTCAAATACGCCggg                                  <  1:1199188/150‑1 (MQ=255)
                                                                                                                     ccaaCAAGCACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCggg                                  >  1:2747604/1‑150 (MQ=255)
                                                                                                                       aacaaGCACCGGTCCCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGtt                                <  1:4283768/150‑1 (MQ=255)
                                                                                                                          aaGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAAt                             >  2:480293/1‑150 (MQ=255)
                                                                                                                           cgccccGGTACCGCGGTGCTTTTTACGACTTCACACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATg                            <  1:1611605/146‑1 (MQ=255)
                                                                                                                           aGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATg                            <  2:1484741/150‑1 (MQ=255)
                                                                                                                            gccccGGTCCCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGc                           <  2:952225/147‑1 (MQ=255)
                                                                                                                            gCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGc                           <  2:2723202/150‑1 (MQ=255)
                                                                                                                             cACCGGTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGcc                          <  2:1008667/150‑1 (MQ=255)
                                                                                                                                 ggTACCGAGGTGCGTTTTACTACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGaa                      >  2:158985/1‑150 (MQ=255)
                                                                                                                                       gAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTgc                >  1:3764041/1‑150 (MQ=255)
                                                                                                                                              gTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGtt         >  2:421981/1‑150 (MQ=255)
                                                                                                                                               ttttACGAATTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGttt        <  2:3276009/150‑1 (MQ=255)
                                                                                                                                                 ttACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTCg      >  2:2356115/1‑150 (MQ=255)
                                                                                                                                                  tACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTCgg     <  1:1407874/150‑1 (MQ=255)
                                                                                                                                                  tACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTCgg     <  1:3560784/150‑1 (MQ=255)
                                                                                                                                                   aCGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTCggg    >  2:3734654/1‑150 (MQ=255)
                                                                                                                                                    cGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTCGGGa   <  1:2422026/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGCCAGTTTCGGGAT  >  CP009273/649992‑650290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑