Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 81,562 C→A 100% I236I (ATC→ATA)  leuO → global transcription factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927381,5620CA95.7% 838.7 / 22.2 253I236I (ATC→ATA) leuOglobal transcription factor
Reads supporting (aligned to +/- strand):  ref base C (6/5);  new base A (144/97);  total (150/103)
Fisher's exact test for biased strand distribution p-value = 7.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 262 total aligned reads displayed.

GCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAACAGCAG  >  CP009273/81415‑81708
                                                                                                                                                   |                                                                                                                                                  
gCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATcgc                                                                                                                                                  >  2:1128625/1‑150 (MQ=255)
 cTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATagcg                                                                                                                                                 <  2:2399558/150‑1 (MQ=255)
    gTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTAt                                                                                                                                              >  2:1756759/1‑150 (MQ=255)
       gCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAg                                                                                                                                           >  1:4223913/1‑150 (MQ=255)
             aaaaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATg                                                                                                                                     <  1:1379281/150‑1 (MQ=255)
                aaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCa                                                                                                                                  >  1:3821633/1‑150 (MQ=255)
                aaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCa                                                                                                                                  >  1:1942033/1‑150 (MQ=255)
                  tcatcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAat                                                                                                                                >  2:1498782/1‑150 (MQ=255)
                    atcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAatga                                                                                                                              <  2:1471223/150‑1 (MQ=255)
                     tcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAatgat                                                                                                                             >  2:3270409/1‑150 (MQ=255)
                     tcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAatgat                                                                                                                             >  1:2760854/1‑150 (MQ=255)
                     tcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAatgat                                                                                                                             <  1:1900883/150‑1 (MQ=255)
                       caacaaTTAAGGGCCCGTTACTGAAACATGATGATTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAAtgatga                                                                                                                           <  2:1999544/150‑1 (MQ=255)
                        aacaaTTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAg                                                                                                                          <  2:3473913/150‑1 (MQ=255)
                              tAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACt                                                                                                                    >  1:3498002/1‑150 (MQ=255)
                                aGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTa                                                                                                                  >  1:2100812/1‑150 (MQ=255)
                                aGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTa                                                                                                                  >  1:2100848/1‑150 (MQ=255)
                                aGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTa                                                                                                                  <  1:2346923/150‑1 (MQ=255)
                                     ccGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCgtg                                                                                                             <  2:1817938/150‑1 (MQ=255)
                                      cGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCgtgg                                                                                                            >  1:1753045/1‑150 (MQ=255)
                                        ttACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCgtggtg                                                                                                          >  2:1686871/1‑150 (MQ=255)
                                          aCTGATACTTGTTGTTTTTTCCGATCTACATGCGGCGGTTTCGCTCGTTCGTTTCTCTTAATTTAGTCATCGTTTGTATGACTCGGTTGATTTGGTAGCCAGTTTTGCGTATGAGGGCATGGCTATGTTGTGCGTTCTTTTCGTGGTGTc                                                                                                        <  2:3645237/150‑1 (MQ=255)
                                            tGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGc                                                                                                      >  2:294703/1‑150 (MQ=255)
                                              aaaCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCaa                                                                                                    >  1:4357534/1‑150 (MQ=255)
                                                aCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAAc                                                                                                  >  1:468995/1‑150 (MQ=255)
                                                aCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCGCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAAc                                                                                                  >  1:1103671/1‑150 (MQ=255)
                                                    gatgTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCAt                                                                                              >  2:983782/1‑150 (MQ=255)
                                                    gatgTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCAt                                                                                              <  1:1565905/150‑1 (MQ=255)
                                                        tttATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTgg                                                                                          >  2:4227697/1‑150 (MQ=255)
                                                         ttATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGt                                                                                         <  2:856140/150‑1 (MQ=255)
                                                          tataACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCTCGTCATTTAGTCAACCTAGGTATGACAACGTATAAAAGCAAGCAAGAATAGCCAAACAGGGCATTTCAATGATGATCTAACTTAGCGTGGTGTACCAAACGAAATTTGTc                                                                                        >  1:661498/1‑150 (MQ=255)
                                                          tataACGAACAACATGCGGCGGTTTAGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATTACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTAATTAGCGTGGTGTCGCAAACGCATTTGGTc                                                                                        >  1:2829762/1‑150 (MQ=255)
                                                           ataACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAATCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCg                                                                                       >  1:2225073/1‑150 (MQ=255)
                                                            taACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGc                                                                                      >  1:3820460/1‑150 (MQ=255)
                                                                    aacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTgcgc                                                                              >  2:104811/1‑150 (MQ=255)
                                                                       aTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTATCGTGGTGTCGCAAACGCATTTGGTCGCTATTTcgccgc                                                                           >  1:840374/1‑150 (MQ=255)
                                                                          cggcggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGtt                                                                        >  1:1056994/1‑150 (MQ=255)
                                                                          cggcggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGtt                                                                        <  2:643716/150‑1 (MQ=255)
                                                                          cggcggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGtt                                                                        >  2:4019727/1‑150 (MQ=255)
                                                                           ggcggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtg                                                                       >  1:1196461/1‑150 (MQ=255)
                                                                            gcggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtgg                                                                      <  2:1091114/150‑1 (MQ=255)
                                                                              ggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtggct                                                                    >  2:3904576/1‑150 (MQ=255)
                                                                              ggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtggct                                                                    <  2:1311308/150‑1 (MQ=255)
                                                                              ggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtggct                                                                    <  1:1213018/150‑1 (MQ=255)
                                                                               gTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtggctg                                                                   >  1:2938617/1‑150 (MQ=255)
                                                                                  tCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTggctggct                                                                <  1:3612922/150‑1 (MQ=255)
                                                                                   cGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGgctggctg                                                               <  2:2562789/150‑1 (MQ=255)
                                                                                   cGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGgctggctg                                                               <  2:2740402/150‑1 (MQ=255)
                                                                                   cGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGgctggctg                                                               <  2:2180289/150‑1 (MQ=255)
                                                                                    gCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGa                                                              >  2:3771198/1‑150 (MQ=255)
                                                                                    gCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGa                                                              >  2:1052396/1‑150 (MQ=255)
                                                                                      tCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAag                                                            <  1:2176039/150‑1 (MQ=255)
                                                                                       cGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAaga                                                           >  2:1189225/1‑150 (MQ=255)
                                                                                        gATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAagag                                                          >  2:3319781/1‑150 (MQ=255)
                                                                                         aTCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGt                                                         >  1:526929/1‑150 (MQ=255)
                                                                                           cGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTc                                                       <  1:1342812/150‑1 (MQ=255)
                                                                                           cGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTc                                                       >  1:377313/1‑150 (MQ=255)
                                                                                           cGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTc                                                       >  1:3251539/1‑150 (MQ=255)
                                                                                             tttCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGc                                                     >  2:4117398/1‑150 (MQ=255)
                                                                                             tttCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGc                                                     >  1:3786648/1‑150 (MQ=255)
                                                                                              ttCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCt                                                    <  1:3323620/150‑1 (MQ=255)
                                                                                                cgcgTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGa                                                  >  1:875435/1‑150 (MQ=255)
                                                                                                  cgTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAAt                                                >  1:4116953/1‑150 (MQ=255)
                                                                                                   gTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATc                                               <  2:556276/150‑1 (MQ=255)
                                                                                                     cATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCt                                             >  2:1632755/1‑150 (MQ=255)
                                                                                                         tAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGa                                         <  2:3824568/150‑1 (MQ=255)
                                                                                                          aGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGaa                                        >  2:669682/1‑150 (MQ=255)
                                                                                                           gTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAAt                                       >  2:4042797/1‑150 (MQ=255)
                                                                                                            tCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAAtt                                      <  2:1605693/150‑1 (MQ=255)
                                                                                                            tCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAAtt                                      <  2:1603696/150‑1 (MQ=255)
                                                                                                             cAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTa                                     <  2:2553927/150‑1 (MQ=255)
                                                                                                              aaCCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTAc                                    >  2:2259569/1‑150 (MQ=255)
                                                                                                                 cTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAgg                                 >  1:3048787/1‑150 (MQ=255)
                                                                                                                 cTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAgg                                 >  1:1319880/1‑150 (MQ=255)
                                                                                                                 cTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAgg                                 <  2:2386078/150‑1 (MQ=255)
                                                                                                                 attGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGAATGGCAATGAAGAGCGTACTTAGAGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTAAGAATTACAgg                                 >  2:4058511/2‑150 (MQ=255)
                                                                                                                    ggTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTAt                              >  2:1515410/1‑150 (MQ=255)
                                                                                                                    ggTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTAt                              <  1:2673653/150‑1 (MQ=255)
                                                                                                                      tATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTa                            <  1:767116/150‑1 (MQ=255)
                                                                                                                         gACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCg                         <  2:957909/150‑1 (MQ=255)
                                                                                                                            acGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTg                      <  2:1419957/150‑1 (MQ=255)
                                                                                                                            acGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTg                      >  1:1293491/1‑150 (MQ=255)
                                                                                                                              ggTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGcc                    >  1:180576/1‑150 (MQ=255)
                                                                                                                              ggTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGcc                    <  2:568445/150‑1 (MQ=255)
                                                                                                                              ggTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGcc                    >  1:177652/1‑150 (MQ=255)
                                                                                                                               gTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCg                   <  2:3383195/150‑1 (MQ=255)
                                                                                                                                tAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGt                  >  2:3703758/1‑150 (MQ=255)
                                                                                                                                 aGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGtt                 <  1:2498633/150‑1 (MQ=255)
                                                                                                                                 aGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGtt                 <  1:3456999/150‑1 (MQ=255)
                                                                                                                                 aGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGtt                 >  1:3078671/1‑150 (MQ=255)
                                                                                                                                 aGATAAGCAAGCCAGTAGAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGtt                 <  1:4038784/150‑1 (MQ=255)
                                                                                                                                  gATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTa                <  1:202365/150‑1 (MQ=255)
                                                                                                                                   aTAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaa               >  2:4217505/1‑150 (MQ=255)
                                                                                                                                        caagcCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaaacaa          >  2:775248/1‑150 (MQ=255)
                                                                                                                                           gcCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaaacaaaac       <  2:51188/150‑1 (MQ=255)
                                                                                                                                            ccAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAaaacaaaaca      >  1:3196281/1‑150 (MQ=255)
                                                                                                                                              aGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAAcagc    >  2:3959132/1‑150 (MQ=255)
                                                                                                                                               gTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAAcagca   <  1:720581/150‑1 (MQ=255)
                                                                                                                                                tataGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAAcagcag  >  1:918937/1‑150 (MQ=255)
                                                                                                                                                tataGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAAcagcag  >  1:2655867/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAACAGCAG  >  CP009273/81415‑81708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑