Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 689,079 C→A 20.1% E445* (GAA→TAA)  miaB ← tRNA‑i(6)A37 methylthiotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273689,0790CA20.1% 693.5 / 126.1 274E445* (GAA→TAA) miaBtRNA‑i(6)A37 methylthiotransferase
Reads supporting (aligned to +/- strand):  ref base C (112/105);  new base A (35/22);  total (147/127)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

AAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCT  >  CP009273/688931‑689228
                                                                                                                                                    |                                                                                                                                                     
  aGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGACTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACTGGCCAACACTGAGTCCGGTTTTTCTGCCACGCGCAGACCCATTTAATc                                                                                                                                                    >  1:172598/1‑150 (MQ=255)
    aTGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCtt                                                                                                                                                  >  1:331496/1‑150 (MQ=255)
    aTGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCtt                                                                                                                                                  <  1:2734210/150‑1 (MQ=255)
    aTGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCtt                                                                                                                                                  >  2:3228390/1‑150 (MQ=255)
    aTGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATAACAGATTCCGGTGTTTCTGCCACGCGCAGACCCAATTCATCtt                                                                                                                                                  >  1:3009874/1‑150 (MQ=255)
     tGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTc                                                                                                                                                 >  2:69581/1‑150 (MQ=255)
      ggggATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCa                                                                                                                                                <  1:908744/150‑1 (MQ=255)
      ggggATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTCa                                                                                                                                                <  2:3821990/150‑1 (MQ=255)
       gggATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCCATCACAGATTACGGTGTTTCTGCCACGCGCAGACCCCATTAATCTTCAg                                                                                                                                               >  1:1103913/1‑150 (MQ=255)
       gggATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAg                                                                                                                                               <  2:2523291/150‑1 (MQ=255)
          aTAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTAc                                                                                                                                            <  1:2027617/150‑1 (MQ=255)
          aTAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTAc                                                                                                                                            >  2:1077287/1‑150 (MQ=255)
           tAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACg                                                                                                                                           >  2:2785811/1‑150 (MQ=255)
           tAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTATGCCACGCGCAGACCCAATTCATCTTCAGTACg                                                                                                                                           >  1:3084433/1‑150 (MQ=255)
            aaaGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGc                                                                                                                                          >  1:814505/1‑150 (MQ=255)
             aaGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTTCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCAATTCATCTTCAGTACGca                                                                                                                                         >  1:2982808/1‑150 (MQ=255)
             aaGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCCATAACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTCAGTACGca                                                                                                                                         >  1:7926/1‑150 (MQ=255)
               gagagaAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGcacc                                                                                                                                       <  1:1271918/150‑1 (MQ=255)
                  agaAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTATTTGCGGGTACGGGCAATCACTGATTACGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTAAGTAAGcaccaca                                                                                                                                    >  1:535689/1‑149 (MQ=255)
                   gaAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACtt                                                                                                                                   >  2:1438724/1‑150 (MQ=255)
                   gaAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTCAGTACGCACCACtt                                                                                                                                   <  1:3358181/150‑1 (MQ=255)
                    aaaaaaCAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACTGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACttt                                                                                                                                  <  1:2882325/150‑1 (MQ=255)
                     aaaaaCAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTa                                                                                                                                 <  2:51569/150‑1 (MQ=255)
                      aaaaCAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTAc                                                                                                                                >  1:228131/1‑150 (MQ=255)
                      aaaaCAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTCAGTACGCACCACTTTAc                                                                                                                                >  1:926416/1‑150 (MQ=255)
                       aaaCAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTAcc                                                                                                                               >  2:1054354/1‑150 (MQ=255)
                        aaCAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACcg                                                                                                                              <  1:2654450/150‑1 (MQ=255)
                        aaCAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACcg                                                                                                                              <  1:482482/150‑1 (MQ=255)
                          cAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACcgcg                                                                                                                            >  2:1958211/1‑150 (MQ=255)
                           aaGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCgcgc                                                                                                                           >  2:3787936/1‑150 (MQ=255)
                              gCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCaga                                                                                                                        <  2:4075712/150‑1 (MQ=255)
                               cccACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTCAGTACGCACCACTTTACCGCGCagag                                                                                                                       >  2:4281102/1‑150 (MQ=255)
                                ccACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCagaga                                                                                                                      <  1:2415894/150‑1 (MQ=255)
                                   ccGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGtt                                                                                                                   <  2:1364382/150‑1 (MQ=255)
                                    cGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTc                                                                                                                  >  2:1816758/1‑150 (MQ=255)
                                        aCGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGt                                                                                                              <  2:4054076/150‑1 (MQ=255)
                                          ggCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTCAGTACGCACCAATTTACCGCGCAGAGAGTTCGGGTAg                                                                                                            >  2:3200553/1‑150 (MQ=255)
                                            cAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGAc                                                                                                          <  1:3816200/150‑1 (MQ=255)
                                               gCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTc                                                                                                       >  2:3070071/1‑150 (MQ=255)
                                               gCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTc                                                                                                       <  1:2446167/150‑1 (MQ=255)
                                                ccTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTAGGGTAGACGTCg                                                                                                      >  1:3152685/1‑150 (MQ=255)
                                                ccTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCg                                                                                                      <  1:1257526/150‑1 (MQ=255)
                                                   gagaATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTa                                                                                                   <  2:2043037/150‑1 (MQ=255)
                                                      aaTTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAAtt                                                                                                <  2:260735/150‑1 (MQ=255)
                                                      aaTTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAAtt                                                                                                <  2:308594/150‑1 (MQ=255)
                                                      aaTTACGGCTGATAATAACCCACGCCAAGGTCGTTTTATTTTCGGGTACGGGCAAAAACAGATTCCGGTGTTTCTGCCACGCGCCGACCCATTTAATCTTCAGTACGCACCACATTACCGCGCAGAGAGTTAGGGTAGACGTCGGTAAtt                                                                                                >  1:4244123/1‑150 (MQ=255)
                                                             gCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACAt                                                                                         <  1:1054992/150‑1 (MQ=255)
                                                              cTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATc                                                                                        >  2:475668/1‑150 (MQ=255)
                                                               tGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATcg                                                                                       >  2:2243667/1‑150 (MQ=255)
                                                                 ataataACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATcgac                                                                                     <  2:1731000/150‑1 (MQ=255)
                                                                  taataaCCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATcgacg                                                                                    >  2:3912234/1‑150 (MQ=255)
                                                                  taataaCCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATcgacg                                                                                    >  2:1399773/1‑150 (MQ=255)
                                                                      aaCCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAAtt                                                                                <  1:1865931/150‑1 (MQ=255)
                                                                      aaCCCACGCCAAGGTCGTGTTCGTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCCCGCGCAGACCCATTTCATCTTCAGTACGCACCACTGTCCCGCGCCGAGAGGTCGGGTAGACGGCGGTAATTTCTACCTCGACGaagt                                                                                >  2:144560/1‑148 (MQ=255)
                                                                           aCGCCAAGGTCGTTTTATTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTAcag                                                                           >  2:3933683/1‑148 (MQ=255)
                                                                             gCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTATACAACGACGAATTTAACGAt                                                                         >  2:753409/1‑150 (MQ=255)
                                                                             gCCAAGGTCGTTTTATTTGCGGGTACGGGCAAACACTGATTCCGGTGTTTCTGCCACGCGCAGACCCAATTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAAATTCTACAACGACGAAATTAcccaa                                                                         >  2:873839/1‑147 (MQ=255)
                                                                              ccAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATc                                                                        <  2:684308/150‑1 (MQ=255)
                                                                               cAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCa                                                                       <  1:4108422/150‑1 (MQ=255)
                                                                                aaGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCat                                                                      <  2:926416/150‑1 (MQ=255)
                                                                                    tCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATcc                                                                  >  2:2940297/1‑150 (MQ=255)
                                                                                      gTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCgg                                                                >  1:3958247/1‑150 (MQ=255)
                                                                                          tCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTg                                                            <  1:1353638/150‑1 (MQ=255)
                                                                                           cTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTCCCGATCATATCCGGCGTGc                                                           >  2:110866/1‑150 (MQ=255)
                                                                                           cTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGc                                                           >  1:21827/1‑150 (MQ=255)
                                                                                               gCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTc                                                       <  1:1077287/150‑1 (MQ=255)
                                                                                                 gggTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGa                                                     >  1:3651247/1‑150 (MQ=255)
                                                                                                 gggTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGa                                                     >  2:1200852/1‑150 (MQ=255)
                                                                                                    tACGGTCAATCTCTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGTGTAGTCGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCTGCGTGCCTTCGAAGt                                                  <  1:4010506/150‑1 (MQ=255)
                                                                                                      cGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTg                                                >  1:1017663/1‑150 (MQ=255)
                                                                                                      cGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTg                                                <  2:3992089/150‑1 (MQ=255)
                                                                                                       gggCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGa                                               >  1:709550/1‑150 (MQ=255)
                                                                                                        ggCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGAc                                              <  1:3235945/150‑1 (MQ=255)
                                                                                                         gCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGAcc                                             <  2:254685/150‑1 (MQ=255)
                                                                                                           aaTCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCAc                                           >  1:2387819/1‑150 (MQ=255)
                                                                                                             tCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCAcgc                                         >  1:2174618/1‑150 (MQ=255)
                                                                                                             tCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCAcgc                                         >  2:3639199/1‑150 (MQ=255)
                                                                                                               aCTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCgg                                       >  1:3131388/1‑150 (MQ=255)
                                                                                                                 tGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGtt                                     >  1:3457090/1‑150 (MQ=255)
                                                                                                                  gATTCCGGTGTTTCTGCCACGCGCAGACCCATTTAATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTa                                    >  1:3724902/1‑150 (MQ=255)
                                                                                                                    gtCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTAtt                                  <  2:140531/149‑1 (MQ=255)
                                                                                                                     tCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTAttt                                 >  2:4175595/1‑150 (MQ=255)
                                                                                                                        ggTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTcc                              >  1:1778866/1‑150 (MQ=255)
                                                                                                                        ggTGTTTCTGCCACGCGCAGACCCATTTAATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTcc                              >  1:2135954/1‑150 (MQ=255)
                                                                                                                              tCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGc                        >  2:1546868/1‑150 (MQ=255)
                                                                                                                               cTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGcc                       <  2:2775782/150‑1 (MQ=255)
                                                                                                                                  ccACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCgg                    <  1:3414863/150‑1 (MQ=255)
                                                                                                                                     cgcgCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGaaa                 >  2:1598028/1‑150 (MQ=255)
                                                                                                                                        gcAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCt              <  2:3434425/150‑1 (MQ=255)
                                                                                                                                         cAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTc             >  2:2878091/1‑150 (MQ=255)
                                                                                                                                         cAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTc             >  1:419516/1‑150 (MQ=255)
                                                                                                                                           gACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCa           <  2:3009610/150‑1 (MQ=255)
                                                                                                                                            aCCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCat          >  1:465742/1‑150 (MQ=255)
                                                                                                                                            aCCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCAGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCat          >  2:3501740/1‑150 (MQ=255)
                                                                                                                                               cagttCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACCTCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCatgat       <  2:2399825/147‑1 (MQ=255)
                                                                                                                                                aTTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCatgatg      >  2:3985660/1‑150 (MQ=255)
                                                                                                                                                  ttCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGct    <  2:4230671/150‑1 (MQ=255)
                                                                                                                                                   tCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGctc   <  1:2249018/150‑1 (MQ=255)
                                                                                                                                                   tCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGctc   <  1:74675/150‑1 (MQ=255)
                                                                                                                                                    cATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGctct  >  2:3701385/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCT  >  CP009273/688931‑689228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑