Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 987,780 C→A 8.4% A568E (GCG→GAG)  pepN → aminopeptidase N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273987,7800CA 8.4% 1016.3 / 48.5 274A568E (GCG→GAG) pepNaminopeptidase N
Reads supporting (aligned to +/- strand):  ref base C (132/119);  new base A (11/12);  total (143/131)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 281 total aligned reads displayed.

CTTTGTCTTTGATAATGTCTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTGGCGGCAGAAATCCT  >  CP009273/987631‑987927
                                                                                                                                                     |                                                                                                                                                   
cTTTGTCTTTGATAATGTCTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATgcggc                                                                                                                                                     <  2:3103502/150‑1 (MQ=255)
 tttGTCTTTGATAATGTCTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATgcggcg                                                                                                                                                    <  1:1851073/150‑1 (MQ=255)
   tGTCTTTGATAATGTCTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCa                                                                                                                                                  <  1:3790842/150‑1 (MQ=255)
       tttGATAATGTCTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGt                                                                                                                                              <  1:3281139/150‑1 (MQ=255)
        ttGATAATGTCTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGtt                                                                                                                                             >  1:2310545/1‑150 (MQ=255)
         tGATAATGTCTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGttt                                                                                                                                            <  2:2616156/150‑1 (MQ=255)
            tAATGTCTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCt                                                                                                                                         <  1:2424148/150‑1 (MQ=255)
            tAATGTCTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCt                                                                                                                                         >  2:4202013/1‑150 (MQ=255)
             aaTGTCTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTg                                                                                                                                        <  1:3935328/150‑1 (MQ=255)
             aaTGTCTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTg                                                                                                                                        >  1:2315476/1‑150 (MQ=255)
              aTGTCTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTgg                                                                                                                                       <  1:927655/150‑1 (MQ=255)
              aTGTCTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTgg                                                                                                                                       <  2:2476647/150‑1 (MQ=255)
                  cTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTTATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAAc                                                                                                                                   <  2:688571/150‑1 (MQ=255)
                  cTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATTATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAAc                                                                                                                                   >  1:3671814/1‑150 (MQ=255)
                  cTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAAc                                                                                                                                   >  2:3153448/1‑150 (MQ=255)
                    aCTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCt                                                                                                                                 >  2:2628568/1‑150 (MQ=255)
                    aCTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGAGCAAAGTTTGCTGGCAACCt                                                                                                                                 >  1:3642092/1‑150 (MQ=255)
                      ttCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGAGGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTAc                                                                                                                               >  1:4053919/1‑150 (MQ=255)
                       tCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACa                                                                                                                              >  1:2820748/1‑150 (MQ=255)
                       tCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGAGCAAAGTTTGCTGGCAACCTACa                                                                                                                              <  2:3265912/150‑1 (MQ=255)
                        ccAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACAt                                                                                                                             <  1:1966810/150‑1 (MQ=255)
                                   cTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACg                                                                                                                  <  1:482568/150‑1 (MQ=255)
                                     gcgcTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTc                                                                                                                <  1:1988049/150‑1 (MQ=255)
                                     gcgcTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTc                                                                                                                <  1:1121356/150‑1 (MQ=255)
                                     gcgcTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTc                                                                                                                <  2:499424/150‑1 (MQ=255)
                                     gcgcTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTc                                                                                                                <  2:3460892/150‑1 (MQ=255)
                                      cgcTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGAGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTcg                                                                                                               >  1:2475702/1‑150 (MQ=255)
                                        ctgctgTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTcgcg                                                                                                             <  1:2661699/150‑1 (MQ=255)
                                        ctgctgTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTcgcg                                                                                                             <  1:3191692/150‑1 (MQ=255)
                                                cGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGtcatca                                                                                                     >  1:639619/1‑150 (MQ=255)
                                                  aaTTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGc                                                                                                   >  2:3583519/1‑150 (MQ=255)
                                                   aTTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCa                                                                                                  >  1:3911471/1‑150 (MQ=255)
                                                   aTTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCa                                                                                                  >  1:3911316/1‑150 (MQ=255)
                                                   aTTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCa                                                                                                  >  1:4175615/1‑150 (MQ=255)
                                                       tctGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGt                                                                                              >  1:2749855/1‑150 (MQ=255)
                                                             ccAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCg                                                                                        >  2:1246343/1‑150 (MQ=255)
                                                             ccAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCg                                                                                        >  1:699196/1‑150 (MQ=255)
                                                               aGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCt                                                                                      >  2:3398649/1‑150 (MQ=255)
                                                               aGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCt                                                                                      >  2:2257524/1‑150 (MQ=255)
                                                                   aaaCTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGtct                                                                                  >  1:772631/1‑150 (MQ=255)
                                                                   aaaCTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGtct                                                                                  >  2:3806086/1‑150 (MQ=255)
                                                                    aaCTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGtctc                                                                                 >  1:3331945/1‑150 (MQ=255)
                                                                    aaCTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGtctc                                                                                 >  1:2488464/1‑150 (MQ=255)
                                                                    aaCTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGtctc                                                                                 >  1:1187544/1‑150 (MQ=255)
                                                                     aCTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTctct                                                                                <  1:3601715/150‑1 (MQ=255)
                                                                       tGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGAGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGc                                                                              >  2:2850684/1‑150 (MQ=255)
                                                                        ggAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCACGGTCAGCCGCTGTCTCTGcc                                                                             >  2:2951201/1‑150 (MQ=255)
                                                                        ggAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGcc                                                                             >  2:1785970/1‑150 (MQ=255)
                                                                        ggAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCAAGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGACAGCCGCTGTCTCTGcc                                                                             >  2:3972429/1‑150 (MQ=255)
                                                                             ataAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGAGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGc                                                                        >  1:542756/1‑150 (MQ=255)
                                                                                aGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCAtg                                                                     >  2:791568/1‑150 (MQ=255)
                                                                                   ggAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTgg                                                                  <  2:156940/150‑1 (MQ=255)
                                                                                    gAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGc                                                                 <  2:1878760/150‑1 (MQ=255)
                                                                                     aGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCt                                                                <  2:13106/150‑1 (MQ=255)
                                                                                      gCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTg                                                               <  1:2908354/150‑1 (MQ=255)
                                                                                        gATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGAt                                                             <  1:1463798/150‑1 (MQ=255)
                                                                                          tCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGc                                                           >  1:2138124/1‑150 (MQ=255)
                                                                                           cAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCt                                                          >  2:1514673/1‑150 (MQ=255)
                                                                                            aGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCtt                                                         <  1:1030269/150‑1 (MQ=255)
                                                                                             gCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCttt                                                        <  2:1616439/150‑1 (MQ=255)
                                                                                              cAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTc                                                       <  1:2963023/150‑1 (MQ=255)
                                                                                              cAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTc                                                       <  2:2576123/150‑1 (MQ=255)
                                                                                              cAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTc                                                       >  1:1710517/1‑150 (MQ=255)
                                                                                                aCTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCcg                                                     >  2:2391501/1‑150 (MQ=255)
                                                                                                aCTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCcg                                                     >  2:2293642/1‑150 (MQ=255)
                                                                                                     ccTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGAGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCgg                                                >  1:3399360/1‑150 (MQ=255)
                                                                                                      cTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGt                                               >  1:162254/1‑150 (MQ=255)
                                                                                                         ccTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACt                                            >  1:153287/1‑150 (MQ=255)
                                                                                                            gATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACCTCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCt                                         <  2:870296/150‑1 (MQ=255)
                                                                                                            gATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCt                                         >  1:2076208/1‑150 (MQ=255)
                                                                                                            gATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCt                                         >  2:1254222/1‑150 (MQ=255)
                                                                                                            gATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGAGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGGACTGCt                                         >  2:4296194/1‑150 (MQ=255)
                                                                                                             aTGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCtt                                        <  1:4279344/150‑1 (MQ=255)
                                                                                                             aTGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCtt                                        <  1:3037990/150‑1 (MQ=255)
                                                                                                              tGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTtg                                       <  2:2586673/150‑1 (MQ=255)
                                                                                                                cGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTtgat                                     >  1:2350265/1‑150 (MQ=255)
                                                                                                                cGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTtgat                                     >  1:4115754/1‑150 (MQ=255)
                                                                                                                   cATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATgag                                  <  2:1174794/150‑1 (MQ=255)
                                                                                                                   cATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATgag                                  <  2:2657940/150‑1 (MQ=255)
                                                                                                                     tGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGagaa                                <  1:3583445/150‑1 (MQ=255)
                                                                                                                       cgcgTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGAGTCATCAGCAAGGTCAGCCGCTTTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGagaaga                              <  1:4218567/150‑1 (MQ=255)
                                                                                                                         cgTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGAtt                            <  1:592979/150‑1 (MQ=255)
                                                                                                                          gTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTg                           <  2:290732/150‑1 (MQ=255)
                                                                                                                          gTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTg                           <  2:2103130/150‑1 (MQ=255)
                                                                                                                                aTTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAg                     >  2:1381710/1‑150 (MQ=255)
                                                                                                                                  ttCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAgcg                   <  1:2619344/150‑1 (MQ=255)
                                                                                                                                  ttCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAgcg                   >  1:320908/1‑150 (MQ=255)
                                                                                                                                  ttCTCCCGCTGGGATGCGGAGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAgcg                   <  2:635705/150‑1 (MQ=255)
                                                                                                                                   tctcCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAgcgc                  >  1:1016649/1‑150 (MQ=255)
                                                                                                                                     tcCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTg                >  2:3287004/1‑150 (MQ=255)
                                                                                                                                     tcCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTg                >  2:4044190/1‑150 (MQ=255)
                                                                                                                                        cGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTggcg             <  2:1968397/150‑1 (MQ=255)
                                                                                                                                        cGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTggcg             >  2:113694/1‑150 (MQ=255)
                                                                                                                                          cTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTggcggc           >  1:259036/1‑150 (MQ=255)
                                                                                                                                           tGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTGGCGGCa          <  1:4279695/150‑1 (MQ=255)
                                                                                                                                           tGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTGGCGGCa          >  1:3944272/1‑150 (MQ=255)
                                                                                                                                               aTGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTGGCGGCAGaaa      >  1:1884780/1‑150 (MQ=255)
                                                                                                                                                tGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTGGCGGCAGAAAt     <  2:3860904/150‑1 (MQ=255)
                                                                                                                                                tGCGGAGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTGGCGGCAGAAAt     <  2:3646183/150‑1 (MQ=255)
                                                                                                                                                   ggcgCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTGGCGGCAGAAATCCt  <  1:1382037/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CTTTGTCTTTGATAATGTCTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTGGCGGCAGAAATCCT  >  CP009273/987631‑987927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑