Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,301,604 A→T 20.5% I433I (ATT→ATA)  clsA ← cardiolipin synthase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,301,6040AT20.5% 540.1 / 148.0 234I433I (ATT→ATA) clsAcardiolipin synthase 1
Reads supporting (aligned to +/- strand):  ref base A (87/99);  new base T (26/22);  total (113/121)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.40e-01

Only 100 of 242 total aligned reads displayed.

CTGAAGAAGTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAA  >  CP009273/1301455‑1301753
                                                                                                                                                     |                                                                                                                                                     
cTGAAGAAGTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTa                                                                                                                                                       >  2:2836797/1‑150 (MQ=255)
 tGAAGAAGTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTaa                                                                                                                                                      <  1:993618/150‑1 (MQ=255)
 tGAAGAAGTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTaa                                                                                                                                                      <  1:3180920/150‑1 (MQ=255)
  gaagaaGTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAAt                                                                                                                                                     <  1:3615565/150‑1 (MQ=255)
  gaagaaGTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAAt                                                                                                                                                     <  2:4039318/150‑1 (MQ=255)
   aagaagTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAAtc                                                                                                                                                    <  2:2228164/150‑1 (MQ=255)
   aagaagTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAAtc                                                                                                                                                    >  2:4349559/1‑150 (MQ=255)
     gaagTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAAtctc                                                                                                                                                  <  1:3039877/150‑1 (MQ=255)
      aagTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCg                                                                                                                                                 <  1:1083133/150‑1 (MQ=255)
      aagTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCg                                                                                                                                                 >  2:4113278/1‑150 (MQ=255)
       agTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGa                                                                                                                                                >  2:2381138/1‑150 (MQ=255)
         tAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGaaa                                                                                                                                              <  1:485633/150‑1 (MQ=255)
           aaaCAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAAtt                                                                                                                                            <  2:1473089/150‑1 (MQ=255)
           aaaCAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAAtt                                                                                                                                            <  1:3421396/150‑1 (MQ=255)
           aaaCAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAAtt                                                                                                                                            >  1:1221906/1‑150 (MQ=255)
            aaCAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAAttt                                                                                                                                           <  2:486075/150‑1 (MQ=255)
             aCAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTa                                                                                                                                          <  2:2808187/150‑1 (MQ=255)
               aGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGc                                                                                                                                        <  1:1926916/150‑1 (MQ=255)
               aGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGc                                                                                                                                        <  1:2244973/150‑1 (MQ=255)
                    cTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAg                                                                                                                                   <  2:3255778/150‑1 (MQ=255)
                      cGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGAc                                                                                                                                 >  2:2192683/1‑150 (MQ=255)
                      cGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGAc                                                                                                                                 <  2:1303961/150‑1 (MQ=255)
                        gCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTa                                                                                                                               >  2:1006198/1‑150 (MQ=255)
                         cGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTAc                                                                                                                              >  2:1255378/1‑150 (MQ=255)
                           acacGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTTCCAGGGTAATCTCGAAATTTAGCCACAGACTAccc                                                                                                                            >  1:2564036/1‑148 (MQ=255)
                            cacGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCa                                                                                                                           <  1:2597142/150‑1 (MQ=255)
                               gTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCatat                                                                                                                        >  1:1010287/1‑150 (MQ=255)
                                 tGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCa                                                                                                                      <  1:367656/150‑1 (MQ=255)
                                    cATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTTCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAgg                                                                                                                   >  2:1548454/1‑150 (MQ=255)
                                      tAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGtt                                                                                                                 <  1:2481061/150‑1 (MQ=255)
                                      tAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGtt                                                                                                                 <  1:1696088/150‑1 (MQ=255)
                                       aaTGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTa                                                                                                                <  1:3343222/150‑1 (MQ=255)
                                          ggACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACt                                                                                                             <  1:1441763/150‑1 (MQ=255)
                                             cGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACtgtg                                                                                                          <  2:3605157/150‑1 (MQ=255)
                                               ttttAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGcc                                                                                                        >  1:1385660/1‑150 (MQ=255)
                                                  tAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAAc                                                                                                     >  2:80945/1‑150 (MQ=255)
                                                  tAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAAc                                                                                                     >  1:581400/1‑150 (MQ=255)
                                                  tAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAAc                                                                                                     <  2:2111298/150‑1 (MQ=255)
                                                   aGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAAcc                                                                                                    <  1:135253/150‑1 (MQ=255)
                                                    gCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGAGTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCa                                                                                                   <  2:3764146/150‑1 (MQ=255)
                                                    gCCATAAACGGGCATCGAGCAGACGAGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCa                                                                                                   <  2:1222562/150‑1 (MQ=255)
                                                      cATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGa                                                                                                 >  2:1616510/1‑150 (MQ=255)
                                                      cATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGa                                                                                                 >  2:1429166/1‑150 (MQ=255)
                                                      cATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGa                                                                                                 >  2:2302796/1‑150 (MQ=255)
                                                       aTAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTATCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGAc                                                                                                >  2:3598442/1‑150 (MQ=255)
                                                       aTAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGAc                                                                                                >  1:940866/1‑150 (MQ=255)
                                                         aaaCGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACtt                                                                                              >  1:1156720/1‑150 (MQ=255)
                                                         aaaCGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGAGTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACtt                                                                                              >  2:2653356/1‑150 (MQ=255)
                                                             gggCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGtt                                                                                          >  2:4193930/1‑150 (MQ=255)
                                                              ggCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTc                                                                                         <  2:1916763/150‑1 (MQ=255)
                                                               gtaTCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCg                                                                                        >  2:4156676/3‑150 (MQ=255)
                                                                   cGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCAt                                                                                    <  1:1756845/150‑1 (MQ=255)
                                                                    gAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATc                                                                                   >  1:649238/1‑150 (MQ=255)
                                                                    gAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATc                                                                                   >  2:103849/1‑150 (MQ=255)
                                                                      gCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGa                                                                                 <  1:2338381/150‑1 (MQ=255)
                                                                        aGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGAcc                                                                               <  1:2755147/150‑1 (MQ=255)
                                                                        aGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGAcc                                                                               >  1:4153859/1‑150 (MQ=255)
                                                                             tGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCAc                                                                          <  1:1270067/150‑1 (MQ=255)
                                                                             tGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCAc                                                                          <  2:3103923/150‑1 (MQ=255)
                                                                               aaCGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGc                                                                        >  1:3584112/1‑150 (MQ=255)
                                                                                  gcgAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGctct                                                                     <  2:1959775/150‑1 (MQ=255)
                                                                                      aaTATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGt                                                                 >  2:1654502/1‑150 (MQ=255)
                                                                                            aTCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAg                                                           >  2:1706395/1‑150 (MQ=255)
                                                                                            aTCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAg                                                           >  1:202701/1‑150 (MQ=255)
                                                                                            aTCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAg                                                           >  1:3504089/1‑150 (MQ=255)
                                                                                                  cTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAAccc                                                     <  1:3378503/150‑1 (MQ=255)
                                                                                                    gAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGc                                                   >  2:2390893/1‑150 (MQ=255)
                                                                                                      aCGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGTCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCt                                                 <  1:2915724/150‑1 (MQ=255)
                                                                                                      aCGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGTCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCt                                                 <  1:1974957/150‑1 (MQ=255)
                                                                                                      aCGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCt                                                 <  2:3568491/150‑1 (MQ=255)
                                                                                                       cggcggCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCtt                                                <  1:4113278/150‑1 (MQ=255)
                                                                                                         gcggcgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCa                                              <  1:2188439/150‑1 (MQ=255)
                                                                                                         gcggcgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCa                                              <  1:90053/150‑1 (MQ=255)
                                                                                                          cggcgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCaa                                             <  1:3287197/150‑1 (MQ=255)
                                                                                                             cgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACt                                          <  2:850027/150‑1 (MQ=255)
                                                                                                              gAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTg                                         >  2:2524046/1‑150 (MQ=255)
                                                                                                              gAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTg                                         <  2:3920145/150‑1 (MQ=255)
                                                                                                              gAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTg                                         >  1:3860810/1‑150 (MQ=255)
                                                                                                                ggTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGAt                                       >  1:270166/1‑150 (MQ=255)
                                                                                                                    aGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAAt                                   <  2:3405979/150‑1 (MQ=255)
                                                                                                                        ccAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTa                               <  1:4305119/150‑1 (MQ=255)
                                                                                                                        ccAAAACCTTGATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTa                               <  2:3675053/150‑1 (MQ=255)
                                                                                                                         cAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTaa                              <  1:2289616/150‑1 (MQ=255)
                                                                                                                          aaaaCCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAAc                             >  1:4214513/1‑150 (MQ=255)
                                                                                                                          aaaaCCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAAc                             <  2:322075/150‑1 (MQ=255)
                                                                                                                           aaaCCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAAcc                            <  2:2850054/150‑1 (MQ=255)
                                                                                                                             aCCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAAcccc                          <  1:4349559/150‑1 (MQ=255)
                                                                                                                                 ttATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagca                      >  1:2091492/1‑150 (MQ=255)
                                                                                                                                  tATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcag                     <  1:3014334/150‑1 (MQ=255)
                                                                                                                                  tATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcag                     <  2:4272397/150‑1 (MQ=255)
                                                                                                                                    tcgtcgATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagcc                   >  1:3354921/1‑150 (MQ=255)
                                                                                                                                     cgtcgATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagcca                  <  1:3850255/150‑1 (MQ=255)
                                                                                                                                     cgtcgATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagcca                  >  2:3030412/1‑150 (MQ=255)
                                                                                                                                       tcgATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagccagc                >  2:3491252/1‑150 (MQ=255)
                                                                                                                                        cgATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagccagca               >  1:1534222/1‑150 (MQ=255)
                                                                                                                                            tGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTc           >  2:1042024/1‑150 (MQ=255)
                                                                                                                                             gCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTcc          <  1:1831231/150‑1 (MQ=255)
                                                                                                                                               cAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGt        >  1:715967/1‑150 (MQ=255)
                                                                                                                                               cAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTACCCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGt        >  1:407/1‑150 (MQ=255)
                                                                                                                                                aGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTa       >  1:1041704/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGAAGAAGTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAA  >  CP009273/1301455‑1301753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑