Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,533,940 C→T 7.4% A970T (GCG→ACG)  narZ ← nitrate reductase 2 (NRZ), alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,533,9400CT 7.4% 1135.1 / 53.0 322A970T (GCG→ACG) narZnitrate reductase 2 (NRZ), alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (148/150);  new base T (13/11);  total (161/161)
Fisher's exact test for biased strand distribution p-value = 8.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 322 total aligned reads displayed.

TGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCATCCTG  >  CP009273/1533792‑1534087
                                                                                                                                                    |                                                                                                                                                   
tGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCt                                                                                                                                                    >  1:1762152/1‑150 (MQ=255)
tGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCt                                                                                                                                                    >  1:2608842/1‑150 (MQ=255)
 gATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCtt                                                                                                                                                   >  1:1837805/1‑150 (MQ=255)
   tCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTg                                                                                                                                                 <  1:2318573/150‑1 (MQ=255)
    cGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGa                                                                                                                                                >  2:270158/1‑150 (MQ=255)
      cTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACt                                                                                                                                              <  2:4001503/150‑1 (MQ=255)
        ttCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGc                                                                                                                                            >  1:2387016/1‑150 (MQ=255)
         tCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCa                                                                                                                                           >  1:1008842/1‑150 (MQ=255)
          ccAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGACAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCaa                                                                                                                                          <  2:1044621/150‑1 (MQ=255)
          ccAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCaa                                                                                                                                          <  2:333136/150‑1 (MQ=255)
             gACCGGACCAGGTGGGGCTGGAGATAATGTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACag                                                                                                                                       <  2:3111837/150‑2 (MQ=255)
              aCCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATg                                                                                                                                      >  2:4083124/1‑150 (MQ=255)
               ccGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGa                                                                                                                                     <  2:1544236/150‑1 (MQ=255)
                cGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGAc                                                                                                                                    <  1:1701289/150‑1 (MQ=255)
                 ggACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGAcc                                                                                                                                   >  1:1075855/1‑150 (MQ=255)
                   aCCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGTTTTGACTGCAACATGACCGt                                                                                                                                 >  2:3508358/1‑150 (MQ=255)
                   aCCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGt                                                                                                                                 <  1:3821184/150‑1 (MQ=255)
                   aCCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGt                                                                                                                                 >  2:621563/1‑150 (MQ=255)
                       ggTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGt                                                                                                                             <  2:306837/150‑1 (MQ=255)
                        gTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGtt                                                                                                                            <  1:487200/150‑1 (MQ=255)
                          ggggCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTc                                                                                                                          <  1:2841534/150‑1 (MQ=255)
                           gggCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTcc                                                                                                                         <  2:3608345/150‑1 (MQ=255)
                              cTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGgt                                                                                                                      <  2:2191611/150‑1 (MQ=255)
                                ggAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGgtgc                                                                                                                    >  1:2353933/1‑150 (MQ=255)
                                   gaTAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGgtgccag                                                                                                                 <  1:3500210/150‑1 (MQ=255)
                                      aaTTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGgtgccagtgc                                                                                                              >  2:2321929/1‑150 (MQ=255)
                                            aCGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAAt                                                                                                        <  2:3691889/150‑1 (MQ=255)
                                            aCGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAAt                                                                                                        >  2:2791266/1‑150 (MQ=255)
                                              gcgGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCa                                                                                                      <  1:4298399/150‑1 (MQ=255)
                                              gcgGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCa                                                                                                      <  2:1736732/150‑1 (MQ=255)
                                               cgGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCAc                                                                                                     >  2:1234407/1‑150 (MQ=255)
                                               cgGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCAc                                                                                                     >  2:3427766/1‑150 (MQ=255)
                                                ggCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACt                                                                                                    >  2:2741278/1‑150 (MQ=255)
                                                   tGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCt                                                                                                 >  2:1197566/1‑150 (MQ=255)
                                                     cgcCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGa                                                                                               <  1:2141068/150‑1 (MQ=255)
                                                      gcCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGaa                                                                                              >  1:973604/1‑150 (MQ=255)
                                                      gcCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGaa                                                                                              <  1:708561/150‑1 (MQ=255)
                                                        cTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGTTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGc                                                                                            <  1:3557744/150‑1 (MQ=255)
                                                                cgcgAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATgg                                                                                    <  2:3556861/150‑1 (MQ=255)
                                                                 gcgAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGc                                                                                   >  1:628345/1‑150 (MQ=255)
                                                                     aaGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGgtg                                                                               >  2:798853/1‑150 (MQ=255)
                                                                      aGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGgtgt                                                                              >  1:3790010/1‑150 (MQ=255)
                                                                      aGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGgtgt                                                                              >  1:1599098/1‑150 (MQ=255)
                                                                         gAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGTTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCaa                                                                           >  1:2899002/1‑150 (MQ=255)
                                                                         gAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCaa                                                                           >  2:1410941/1‑150 (MQ=255)
                                                                          aaTCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAAt                                                                          >  1:3012445/1‑150 (MQ=255)
                                                                           aTCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATc                                                                         >  2:1106750/1‑150 (MQ=255)
                                                                            tcttctCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCa                                                                        <  2:1316386/150‑1 (MQ=255)
                                                                             cttctCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAg                                                                       <  1:3956103/150‑1 (MQ=255)
                                                                               tctcGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCg                                                                     <  2:3361136/150‑1 (MQ=255)
                                                                                  cGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGAc                                                                  <  1:3689974/150‑1 (MQ=255)
                                                                                    tCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGc                                                                >  2:500097/1‑150 (MQ=255)
                                                                                        ctTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGTTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCt                                                            >  2:74883/1‑150 (MQ=255)
                                                                                        ctTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCt                                                            >  1:98290/1‑150 (MQ=255)
                                                                                           tgtgCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCtgtg                                                         <  2:3348367/150‑1 (MQ=255)
                                                                                             tgCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGcc                                                       >  1:780134/1‑150 (MQ=255)
                                                                                                aGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCggg                                                    >  2:110912/1‑150 (MQ=255)
                                                                                                  cgcCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGcc                                                  >  2:3939570/1‑150 (MQ=255)
                                                                                                   gcCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCa                                                 >  1:3749012/1‑150 (MQ=255)
                                                                                                   gcCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCa                                                 >  1:4361128/1‑150 (MQ=255)
                                                                                                      aGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCa                                              <  1:1406924/150‑1 (MQ=255)
                                                                                                          gggTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCt                                          <  2:1906726/150‑1 (MQ=255)
                                                                                                          gggTATGGTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCt                                          <  2:695834/150‑1 (MQ=255)
                                                                                                           ggTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCtt                                         >  2:2267352/1‑150 (MQ=255)
                                                                                                           ggTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGAAGGTTTCCGGTGCCAGTGCCAGAATCACTTCAGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGAGCCGGGCCAACACGCta                                         >  2:3239147/1‑149 (MQ=255)
                                                                                                            gTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTg                                        >  1:1808796/1‑150 (MQ=255)
                                                                                                             tATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTgg                                       <  1:3475434/150‑1 (MQ=255)
                                                                                                              aTGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGgt                                      <  1:957805/150‑1 (MQ=255)
                                                                                                               tGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGTTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGgtg                                     >  1:3087824/1‑150 (MQ=255)
                                                                                                                gTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACAGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAAACACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGgtgt                                    >  2:1708448/1‑150 (MQ=255)
                                                                                                                    gcgcCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTACtt                                >  2:3152003/1‑150 (MQ=255)
                                                                                                                    gcgcCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAAtt                                <  2:2830684/150‑1 (MQ=255)
                                                                                                                      gcCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGa                              <  2:725184/150‑1 (MQ=255)
                                                                                                                        ccGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGt                            >  2:1351069/1‑150 (MQ=255)
                                                                                                                        ccGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGt                            >  2:1666034/1‑150 (MQ=255)
                                                                                                                          ggTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGttt                          >  1:1377223/1‑150 (MQ=255)
                                                                                                                          ggTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGttt                          <  2:2135673/150‑1 (MQ=255)
                                                                                                                             gATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGcc                       >  2:2559853/1‑150 (MQ=255)
                                                                                                                             gATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGcc                       >  1:1268813/1‑150 (MQ=255)
                                                                                                                              aTCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCg                      >  1:2799822/1‑150 (MQ=255)
                                                                                                                                  cGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGa                  <  2:3766546/150‑1 (MQ=255)
                                                                                                                                   gCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGaa                 <  1:3815367/150‑1 (MQ=255)
                                                                                                                                    cccAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGaaa                >  1:2615957/1‑150 (MQ=255)
                                                                                                                                     ccAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAAt               <  1:337863/150‑1 (MQ=255)
                                                                                                                                       aGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCg             >  2:2889446/1‑150 (MQ=255)
                                                                                                                                       aGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCg             <  2:1855453/150‑1 (MQ=255)
                                                                                                                                         cgcCTGCCACGTTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGAt           <  1:2710377/150‑1 (MQ=255)
                                                                                                                                         cgcCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGAt           >  2:719594/1‑150 (MQ=255)
                                                                                                                                           ccTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGAttt         >  1:4201530/1‑150 (MQ=255)
                                                                                                                                           ccTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGAttt         >  2:4299484/1‑150 (MQ=255)
                                                                                                                                            cTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTc        <  2:873604/150‑1 (MQ=255)
                                                                                                                                            cTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTc        <  2:1798838/150‑1 (MQ=255)
                                                                                                                                             tGCCACGTTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCa       >  1:2192696/1‑150 (MQ=255)
                                                                                                                                             tGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCa       <  1:115956/150‑1 (MQ=255)
                                                                                                                                             tGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCa       <  1:1898751/150‑1 (MQ=255)
                                                                                                                                             tGCCACGCTTTGACTGCAACATGACCGTTGGTGTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCa       <  1:833337/150‑1 (MQ=255)
                                                                                                                                                 aCGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAACTCGATTTCATCCt   >  1:1555371/1‑150 (MQ=255)
                                                                                                                                                 aCGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCATCCt   <  1:3155056/150‑1 (MQ=255)
                                                                                                                                                  cGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCATCCTg  >  1:1101328/1‑150 (MQ=255)
                                                                                                                                                  cGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCATCCTg  >  1:586742/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAAGCGAATCTTCTCGTCCTCTTTGTGCAGCGCCAGATGGGTATGTTCGCGCCCGGTGATCTCGCCCAGCGCCTGCCACGCTTTGACTGCAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCATCCTG  >  CP009273/1533792‑1534087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑