Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,795,267 A→T 13.5% F521I (TTC→ATC)  thrS ← threonyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,795,2670AT13.5% 880.6 / 109.0 289F521I (TTC→ATC) thrSthreonyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (151/99);  new base T (21/18);  total (172/117)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 291 total aligned reads displayed.

TTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTC  >  CP009273/1795119‑1795414
                                                                                                                                                    |                                                                                                                                                   
ttAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGaaa                                                                                                                                                    <  1:2912047/150‑1 (MQ=255)
 tAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAAc                                                                                                                                                   <  1:4069437/150‑1 (MQ=255)
 tAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAAc                                                                                                                                                   >  2:2188090/1‑150 (MQ=255)
   acacGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGt                                                                                                                                                 >  1:810194/1‑150 (MQ=255)
    cacGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGtt                                                                                                                                                >  2:3861249/1‑150 (MQ=255)
     acGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTc                                                                                                                                               <  1:3028853/150‑1 (MQ=255)
      cGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTcc                                                                                                                                              <  2:1430474/150‑1 (MQ=255)
       gAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCa                                                                                                                                             >  1:1973222/1‑150 (MQ=255)
        aaTGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCAt                                                                                                                                            >  1:437237/1‑150 (MQ=255)
         aTGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACAGGCTCAAGCCAGGTCGGGAAGAAACAAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATc                                                                                                                                           >  2:374544/1‑150 (MQ=255)
          tGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCg                                                                                                                                          <  2:2861951/150‑1 (MQ=255)
          tGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCg                                                                                                                                          >  2:2169116/1‑150 (MQ=255)
          ggCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCg                                                                                                                                          <  2:3957886/149‑1 (MQ=255)
             ccGCATTTGATAGTTTTTGCGTCAATTCTTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGAcc                                                                                                                                       <  2:1334775/150‑1 (MQ=255)
               gCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGAcccc                                                                                                                                     >  2:3977015/1‑150 (MQ=255)
                 aTTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAg                                                                                                                                   >  2:714823/1‑150 (MQ=255)
                      aTAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTg                                                                                                                              >  1:1148228/1‑150 (MQ=255)
                      aTAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTg                                                                                                                              <  2:2554933/150‑1 (MQ=255)
                        aGTTTTTGCGTCAATTCGTTAACGTATTCAGTCTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCTAGCCAGGTCGGGAAGAAACCAGCGTACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTgcg                                                                                                                            >  2:111833/1‑150 (MQ=255)
                        aGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTgcg                                                                                                                            >  2:2286031/1‑150 (MQ=255)
                        aGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTgcg                                                                                                                            <  2:4203069/150‑1 (MQ=255)
                             ttGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTg                                                                                                                       <  2:1974195/150‑1 (MQ=255)
                              tGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGa                                                                                                                      >  1:1088682/1‑150 (MQ=255)
                                 gTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATc                                                                                                                   >  1:2194129/1‑150 (MQ=255)
                                 gTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATc                                                                                                                   >  2:3303927/1‑150 (MQ=255)
                                 gTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATc                                                                                                                   >  1:1027867/1‑150 (MQ=255)
                                     aTTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTa                                                                                                               >  1:1304136/1‑150 (MQ=255)
                                       tCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGACTCATAAcc                                                                                                             >  1:2899777/1‑150 (MQ=255)
                                        cGTTAACGTATTCAGACTGTGAATCGGTAATATTCCTGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCg                                                                                                            <  2:3593497/150‑1 (MQ=255)
                                          ttAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGTAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGTATCGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGt                                                                                                          <  2:575752/150‑1 (MQ=255)
                                          ttAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGt                                                                                                          >  2:4094818/1‑150 (MQ=255)
                                            aaCGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTAc                                                                                                        >  2:870834/1‑150 (MQ=255)
                                             aCGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACt                                                                                                       >  1:900179/1‑150 (MQ=255)
                                             aCGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACt                                                                                                       >  1:246688/1‑150 (MQ=255)
                                              cGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACtt                                                                                                      <  2:623792/150‑1 (MQ=255)
                                               gTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACttt                                                                                                     <  1:495159/150‑1 (MQ=255)
                                               gTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGACTCATTACCGGTACttt                                                                                                     >  1:4113614/1‑150 (MQ=255)
                                                tATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTGTa                                                                                                    >  2:3663866/1‑150 (MQ=255)
                                                 aTTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTAc                                                                                                   >  1:3303055/1‑150 (MQ=255)
                                                 aTTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTAc                                                                                                   >  2:2331111/1‑150 (MQ=255)
                                                    cAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGtt                                                                                                >  1:2712794/1‑150 (MQ=255)
                                                       aCTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCAt                                                                                             <  1:995434/150‑1 (MQ=255)
                                                          gtgAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGt                                                                                          >  1:3451697/1‑150 (MQ=255)
                                                            gAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCt                                                                                        >  2:3172284/1‑150 (MQ=255)
                                                            gAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCt                                                                                        >  1:1106217/1‑150 (MQ=255)
                                                              aTCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTc                                                                                      >  2:2377073/1‑150 (MQ=255)
                                                               tCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCg                                                                                     >  2:476610/1‑150 (MQ=255)
                                                               tCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCg                                                                                     >  1:3204623/1‑150 (MQ=255)
                                                                cGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGc                                                                                    >  2:493864/1‑150 (MQ=255)
                                                                 ggTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGcc                                                                                   <  1:3883750/150‑1 (MQ=255)
                                                                  gTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCt                                                                                  <  1:2281395/150‑1 (MQ=255)
                                                                     atatTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACa                                                                               <  1:4173277/150‑1 (MQ=255)
                                                                     atatTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACa                                                                               <  1:171336/150‑1 (MQ=255)
                                                                      tatTCATGATAACAACCTGCACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACAt                                                                              <  2:2628561/150‑1 (MQ=255)
                                                                      tatTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACAt                                                                              <  1:3667811/150‑1 (MQ=255)
                                                                         tCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaag                                                                           >  2:2104314/1‑150 (MQ=255)
                                                                         tCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaag                                                                           >  1:452051/1‑150 (MQ=255)
                                                                           aTGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaagaa                                                                         >  2:3390161/1‑150 (MQ=255)
                                                                            tGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaagaag                                                                        >  1:1895637/1‑150 (MQ=255)
                                                                            tGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaagaag                                                                        <  2:4333708/150‑1 (MQ=255)
                                                                            tGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaagaag                                                                        <  1:724582/150‑1 (MQ=255)
                                                                             gATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAgc                                                                       <  1:1831063/150‑1 (MQ=255)
                                                                              aTAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTCCCGGTCCTTTACGTTCATTGTCTTCGCCTCCATAAGAAgcg                                                                      >  2:4021020/1‑150 (MQ=255)
                                                                              aTAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAgcg                                                                      >  2:1900989/1‑150 (MQ=255)
                                                                                  caacCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCa                                                                  >  1:1997858/1‑150 (MQ=255)
                                                                                   aacCTGAACCGGCGCAAGCCAGGTCGTGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGGTCATTGTCTTCGCCTACATAAGAAGCGCTccg                                                                 <  1:1914551/150‑3 (MQ=255)
                                                                                   aacCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCag                                                                 >  1:2693585/1‑150 (MQ=255)
                                                                                   aacCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCag                                                                 >  2:2923352/1‑150 (MQ=255)
                                                                                    acCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCaga                                                                <  1:1531853/150‑1 (MQ=255)
                                                                                    acCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCaga                                                                >  2:2874609/1‑150 (MQ=255)
                                                                                      cTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCagacg                                                              >  2:1877982/1‑150 (MQ=255)
                                                                                      cTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCagacg                                                              >  2:3528337/1‑150 (MQ=255)
                                                                                            cGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACgg                                                        >  1:345543/1‑150 (MQ=255)
                                                                                              gcgcAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCa                                                      <  1:1422141/150‑1 (MQ=255)
                                                                                              gcgcAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGACGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCa                                                      <  1:1586724/150‑1 (MQ=255)
                                                                                                   aGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAagag                                                 >  1:1239005/1‑150 (MQ=255)
                                                                                                    gCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCGGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATGAGAAGCGCTCAGACGAGACGGCAAAgaga                                                >  2:1594456/1‑150 (MQ=255)
                                                                                                      cAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAg                                              <  2:2349456/150‑1 (MQ=255)
                                                                                                       aGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTCCTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGt                                             >  1:2387352/1‑150 (MQ=255)
                                                                                                       aGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGt                                             >  1:3400181/1‑150 (MQ=255)
                                                                                                       aGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGt                                             >  2:1274312/1‑150 (MQ=255)
                                                                                                         gTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTcc                                           <  1:2167405/150‑1 (MQ=255)
                                                                                                                agaaACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCACAGAGAAGTCCAGctgta                                    >  2:795157/1‑150 (MQ=255)
                                                                                                                agaaACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGctgta                                    >  1:2060233/1‑150 (MQ=255)
                                                                                                                agaaACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGctgta                                    <  1:179368/150‑1 (MQ=255)
                                                                                                                    aCCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGctgtactgt                                >  1:3058071/1‑150 (MQ=255)
                                                                                                                      cAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCtgtactgtac                              >  1:1053985/1‑150 (MQ=255)
                                                                                                                       aGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTAcc                             >  1:2550866/1‑150 (MQ=255)
                                                                                                                           aaCTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCAc                         >  2:3180582/1‑150 (MQ=255)
                                                                                                                            aCTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACt                        >  1:962771/1‑150 (MQ=255)
                                                                                                                            aCTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACt                        >  1:359417/1‑150 (MQ=255)
                                                                                                                             ctctTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTg                       <  2:3302603/150‑1 (MQ=255)
                                                                                                                               cgtCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGcc                     <  2:1339510/148‑1 (MQ=255)
                                                                                                                                   ggTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGc                 <  1:2614247/150‑1 (MQ=255)
                                                                                                                                   ggTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGc                 >  2:2718550/1‑150 (MQ=255)
                                                                                                                                        ggATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGAt            <  1:3775032/150‑1 (MQ=255)
                                                                                                                                         gATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATc           <  1:4290893/150‑1 (MQ=255)
                                                                                                                                               gATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCa     >  1:3130418/1‑150 (MQ=255)
                                                                                                                                               gATGAAACGTTCCATCGACCCCAGAAGTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCa     <  2:2645167/150‑1 (MQ=255)
                                                                                                                                                 tGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGt   <  2:6643/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTC  >  CP009273/1795119‑1795414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑