Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,796,428 C→A 7.5% V134F (GTC→TTC)  thrS ← threonyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,796,4280CA 7.5% 860.8 / 33.3 232V134F (GTC→TTC) thrSthreonyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (106/108);  new base A (14/4);  total (120/112)
Fisher's exact test for biased strand distribution p-value = 2.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 239 total aligned reads displayed.

GCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTT  >  CP009273/1796279‑1796574
                                                                                                                                                     |                                                                                                                                                  
   gCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAcgtc                                                                                                                                                 >  2:2337331/1‑150 (MQ=255)
   gCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAagtc                                                                                                                                                 <  2:266699/150‑1 (MQ=255)
    cacaTATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAagtcg                                                                                                                                                <  2:2767691/150‑1 (MQ=255)
       atatCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAg                                                                                                                                             <  2:1910615/150‑1 (MQ=255)
       atatCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAg                                                                                                                                             <  2:1005042/150‑1 (MQ=255)
          tCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGttt                                                                                                                                          >  2:1735/1‑150 (MQ=255)
           cGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGtttt                                                                                                                                         <  2:4061114/150‑1 (MQ=255)
                tatTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCa                                                                                                                                    <  2:4203002/150‑1 (MQ=255)
                   tcttcATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCa                                                                                                                                 <  1:3031088/150‑1 (MQ=255)
                   tcttcATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCa                                                                                                                                 >  2:4149099/1‑150 (MQ=255)
                    cttcATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCaa                                                                                                                                >  2:2396192/1‑150 (MQ=255)
                    cttcATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCaa                                                                                                                                >  2:1445034/1‑150 (MQ=255)
                      tcATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGc                                                                                                                              >  1:3269348/1‑150 (MQ=255)
                      tcATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGc                                                                                                                              >  1:2965773/1‑150 (MQ=255)
                       cATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCt                                                                                                                             >  2:3329230/1‑150 (MQ=255)
                         tGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCa                                                                                                                           <  2:2943059/150‑1 (MQ=255)
                             aGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCa                                                                                                                       <  2:935546/150‑1 (MQ=255)
                               tACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATc                                                                                                                     <  1:3664767/150‑1 (MQ=255)
                                aCAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATcc                                                                                                                    >  2:3878919/1‑150 (MQ=255)
                                    aCCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCtt                                                                                                                >  2:2592166/1‑150 (MQ=255)
                                      cTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTtct                                                                                                              <  1:3972627/150‑1 (MQ=255)
                                       tGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTtctc                                                                                                             <  1:4283147/150‑1 (MQ=255)
                                       tGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTtctc                                                                                                             >  2:1811386/1‑150 (MQ=255)
                                        ggCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCg                                                                                                            <  1:4041561/150‑1 (MQ=255)
                                           ttGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGt                                                                                                         <  2:2713662/150‑1 (MQ=255)
                                            tGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTg                                                                                                        <  1:2806079/150‑1 (MQ=255)
                                             gTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGc                                                                                                       <  2:3122364/150‑1 (MQ=255)
                                                atcatGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTc                                                                                                    >  1:2163901/1‑150 (MQ=255)
                                                       gCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCt                                                                                             <  1:1663049/150‑1 (MQ=255)
                                                        cGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCtt                                                                                            >  1:3036163/1‑150 (MQ=255)
                                                        cGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCtt                                                                                            >  2:3429105/1‑150 (MQ=255)
                                                          aTGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTcc                                                                                          >  1:168244/1‑150 (MQ=255)
                                                          aTGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTcc                                                                                          >  1:2755602/1‑150 (MQ=255)
                                                            gTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTg                                                                                        >  2:1574578/1‑150 (MQ=255)
                                                            gTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTg                                                                                        >  2:2256188/1‑150 (MQ=255)
                                                                 cGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTa                                                                                   >  2:166795/1‑150 (MQ=255)
                                                                  gTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTaa                                                                                  <  2:2892789/150‑1 (MQ=255)
                                                                  gTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTaa                                                                                  >  1:2606816/1‑150 (MQ=255)
                                                                   tCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAAc                                                                                 >  1:1436580/1‑150 (MQ=255)
                                                                   tCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAAc                                                                                 <  2:2196743/150‑1 (MQ=255)
                                                                   tCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAAc                                                                                 <  2:1232435/150‑1 (MQ=255)
                                                                   tCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAAc                                                                                 <  2:1233238/150‑1 (MQ=255)
                                                                     aaGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACgg                                                                               <  1:782239/150‑2 (MQ=255)
                                                                     aaGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGt                                                                               >  1:2266970/1‑150 (MQ=255)
                                                                       gAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGc                                                                             >  1:2088567/1‑150 (MQ=255)
                                                                       gAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGc                                                                             >  1:4274197/1‑150 (MQ=255)
                                                                        aaTGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCg                                                                            >  2:1649276/1‑150 (MQ=255)
                                                                         aTGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCgg                                                                           >  2:234067/1‑150 (MQ=255)
                                                                         aTGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCgg                                                                           >  2:2223388/1‑150 (MQ=255)
                                                                          tGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGt                                                                          >  2:4099836/1‑150 (MQ=255)
                                                                           ggAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTc                                                                         >  1:2182662/1‑150 (MQ=255)
                                                                               aCTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGa                                                                     <  2:691433/150‑1 (MQ=255)
                                                                                cTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGAt                                                                    <  2:169399/150‑1 (MQ=255)
                                                                                cTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGAt                                                                    >  1:210585/1‑150 (MQ=255)
                                                                                cTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGAt                                                                    <  2:2037650/150‑1 (MQ=255)
                                                                                 tttGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATc                                                                   <  1:4149099/150‑1 (MQ=255)
                                                                                 tttGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATc                                                                   <  2:3574835/150‑1 (MQ=255)
                                                                                  ttGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCa                                                                  <  1:1990166/150‑1 (MQ=255)
                                                                                   tGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCaa                                                                 <  1:716124/150‑1 (MQ=255)
                                                                                   tGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCaa                                                                 <  1:2884861/150‑1 (MQ=255)
                                                                                    gTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAc                                                                <  2:542661/150‑1 (MQ=255)
                                                                                         tctcCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAcgtcgt                                                           >  1:322807/1‑150 (MQ=255)
                                                                                          ctcCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGta                                                          <  1:1792705/150‑1 (MQ=255)
                                                                                          ctcCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGta                                                          >  1:2238441/1‑150 (MQ=255)
                                                                                             cccACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGtaata                                                       >  2:3750460/1‑150 (MQ=255)
                                                                                              ccACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGtaataa                                                      >  1:1083854/1‑150 (MQ=255)
                                                                                                 cGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATaaaaa                                                   <  1:958140/150‑1 (MQ=255)
                                                                                                        cGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGt                                            >  1:3199924/1‑150 (MQ=255)
                                                                                                         gAAAGTTTCTCGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGATTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTc                                           <  1:1117001/150‑1 (MQ=255)
                                                                                                         gAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTc                                           >  1:1892918/1‑150 (MQ=255)
                                                                                                         gAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTc                                           >  1:62712/1‑150 (MQ=255)
                                                                                                         gAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTc                                           >  1:1620930/1‑150 (MQ=255)
                                                                                                          aaaGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCa                                          >  2:1171729/1‑150 (MQ=255)
                                                                                                           aaGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCaa                                         <  1:1009339/150‑1 (MQ=255)
                                                                                                           aaGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCaa                                         >  1:4617/1‑150 (MQ=255)
                                                                                                             gTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATa                                       >  2:3566517/1‑150 (MQ=255)
                                                                                                             gTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATa                                       <  1:827991/150‑1 (MQ=255)
                                                                                                                tCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAAcc                                    <  2:1792363/150‑1 (MQ=255)
                                                                                                                 cACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCg                                   >  1:1492347/1‑150 (MQ=255)
                                                                                                                 cACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCg                                   >  2:1770011/1‑150 (MQ=255)
                                                                                                                    gcgcTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGc                                <  1:2339099/150‑1 (MQ=255)
                                                                                                                     cgcTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGcc                               <  1:136441/150‑1 (MQ=255)
                                                                                                                         tCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGAtt                           <  1:1695468/150‑1 (MQ=255)
                                                                                                                         tCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGAtt                           <  2:3433937/150‑1 (MQ=255)
                                                                                                                               cAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAAACGTTGTCAATAACCGGGCCGATTGCCAtt                     >  1:2909594/1‑150 (MQ=255)
                                                                                                                                 gCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCAtttt                   >  1:279228/1‑150 (MQ=255)
                                                                                                                                  cTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTg                  <  2:1919756/150‑1 (MQ=255)
                                                                                                                                  cTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTg                  <  1:2097878/150‑1 (MQ=255)
                                                                                                                                  cTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTg                  <  1:3366869/150‑1 (MQ=255)
                                                                                                                                      cTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAAACGTTGTCAATAACCGGGCCGATTTCCATTTTTGTAt              >  1:3648182/1‑150 (MQ=255)
                                                                                                                                       tttCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATg             >  1:852042/1‑150 (MQ=255)
                                                                                                                                       tttCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATg             <  1:1649276/150‑1 (MQ=255)
                                                                                                                                         tcttctTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCg           <  2:214695/150‑1 (MQ=255)
                                                                                                                                          cttcttAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCgg          >  2:1178723/1‑150 (MQ=255)
                                                                                                                                           ttcttAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGc         <  2:2055225/150‑1 (MQ=255)
                                                                                                                                            tcttAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGcc        >  1:2500750/1‑150 (MQ=255)
                                                                                                                                                aaTGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAg    >  1:3489588/1‑150 (MQ=255)
                                                                                                                                                 aTGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAgt   <  2:3225880/150‑1 (MQ=255)
                                                                                                                                                 aTGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAgt   <  2:3224978/150‑1 (MQ=255)
                                                                                                                                                  tGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAgtt  >  2:669534/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTT  >  CP009273/1796279‑1796574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑