Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,802,047 G→A 100% intergenic (+100/‑6) ydiZ → / → yniA uncharacterized protein/fructosamine kinase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,802,0470GA95.5% 1029.4 / 22.5 291intergenic (+100/‑6)ydiZ/yniAuncharacterized protein/fructosamine kinase family protein
Reads supporting (aligned to +/- strand):  ref base G (8/5);  new base A (152/124);  total (160/131)
Fisher's exact test for biased strand distribution p-value = 7.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 294 total aligned reads displayed.

GAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGA  >  CP009273/1801900‑1802195
                                                                                                                                                   |                                                                                                                                                    
    tCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAAcatca                                                                                                                                                >  2:2360977/1‑150 (MQ=255)
     cACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAAcatcat                                                                                                                                               <  1:782863/150‑1 (MQ=255)
       ccATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCAtgt                                                                                                                                             <  1:2863694/150‑1 (MQ=255)
       ccATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCAtgt                                                                                                                                             <  1:4128047/150‑1 (MQ=255)
         aTCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTgg                                                                                                                                           >  1:358062/1‑150 (MQ=255)
          tCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTggc                                                                                                                                          >  2:1503711/1‑150 (MQ=255)
          tCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTggc                                                                                                                                          >  1:1782090/1‑150 (MQ=255)
           cACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTggca                                                                                                                                         >  2:1585149/1‑150 (MQ=255)
           cACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTggca                                                                                                                                         <  1:264269/150‑1 (MQ=255)
           cACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTggca                                                                                                                                         <  2:3275981/150‑1 (MQ=255)
              gACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTggcaggc                                                                                                                                      >  2:1900478/1‑150 (MQ=255)
              gACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTggcaggc                                                                                                                                      <  1:357435/150‑1 (MQ=255)
               aCCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTggcaggca                                                                                                                                     >  2:2524340/1‑150 (MQ=255)
                  tGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATc                                                                                                                                  >  1:30150/1‑150 (MQ=255)
                   gggAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCa                                                                                                                                 >  1:3220502/1‑150 (MQ=255)
                   gggAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCa                                                                                                                                 >  1:930371/1‑150 (MQ=255)
                   gggAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCa                                                                                                                                 >  1:3989474/1‑150 (MQ=255)
                     gaggaATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAgt                                                                                                                               <  2:104451/150‑1 (MQ=255)
                      aggaATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAgtc                                                                                                                              <  1:645915/150‑1 (MQ=255)
                           tggaatAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCtt                                                                                                                         >  1:4253534/1‑150 (MQ=255)
                           tggaatAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCtt                                                                                                                         >  2:3430422/1‑150 (MQ=255)
                            ggaatAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCttt                                                                                                                        <  2:1173527/150‑1 (MQ=255)
                            ggaatAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCttt                                                                                                                        >  1:4024379/1‑150 (MQ=255)
                             gaatAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCtttt                                                                                                                       >  1:635555/1‑150 (MQ=255)
                               atAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTga                                                                                                                     >  1:2379875/1‑150 (MQ=255)
                                 aGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTgagc                                                                                                                   >  1:4346502/1‑150 (MQ=255)
                                   cAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTgagcga                                                                                                                 >  2:3232369/1‑150 (MQ=255)
                                   cAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTgagcga                                                                                                                 <  2:3845931/150‑1 (MQ=255)
                                   cAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTgagcga                                                                                                                 >  2:3877600/1‑150 (MQ=255)
                                    aaCAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTgagcgag                                                                                                                >  1:1891153/1‑150 (MQ=255)
                                      cAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACCTGGGAGAGACATCATGTGGCAGGCAATCAGTCGACTTTTGAGCGAGCa                                                                                                              >  2:3094186/1‑150 (MQ=255)
                                      cAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCa                                                                                                              >  1:3454990/1‑150 (MQ=255)
                                        gAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGt                                                                                                            >  2:1886247/1‑150 (MQ=255)
                                        gAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGt                                                                                                            >  2:2933553/1‑150 (MQ=255)
                                        gAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGt                                                                                                            >  1:3233164/1‑150 (MQ=255)
                                          aaataaatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTa                                                                                                          >  1:2812333/1‑150 (MQ=255)
                                          aaataaatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTa                                                                                                          >  2:1776615/1‑150 (MQ=255)
                                             taaatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGt                                                                                                       >  1:19164/1‑150 (MQ=255)
                                             taaatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGt                                                                                                       >  2:3154712/1‑150 (MQ=255)
                                              aaatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTg                                                                                                      >  1:3777140/1‑150 (MQ=255)
                                               aatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGa                                                                                                     >  2:1694648/1‑150 (MQ=255)
                                               aatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGa                                                                                                     >  1:1760546/1‑150 (MQ=255)
                                                  ggTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAgg                                                                                                  >  1:835960/1‑150 (MQ=255)
                                                  ggTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAgg                                                                                                  <  2:4012865/150‑1 (MQ=255)
                                                   gTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGc                                                                                                 >  2:991478/1‑150 (MQ=255)
                                                   gTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGc                                                                                                 >  1:2570878/1‑150 (MQ=255)
                                                     ttttGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGa                                                                                               <  1:2209580/150‑1 (MQ=255)
                                                         gggCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATc                                                                                           >  2:2174615/1‑150 (MQ=255)
                                                          ggCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCg                                                                                          >  1:1856951/1‑150 (MQ=255)
                                                            cAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGaa                                                                                        >  1:1355963/1‑150 (MQ=255)
                                                            cAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGAAGTTAGGTGAAGGCGAAATCGaa                                                                                        >  2:2184884/1‑150 (MQ=255)
                                                              ataatCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACt                                                                                      >  2:1808176/1‑150 (MQ=255)
                                                              ataatCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACt                                                                                      <  2:72215/150‑1 (MQ=255)
                                                               taatCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTg                                                                                     >  2:1088003/1‑150 (MQ=255)
                                                               taatCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTg                                                                                     >  2:424292/1‑150 (MQ=255)
                                                               taatCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTg                                                                                     >  2:655121/1‑150 (MQ=255)
                                                                aatCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGc                                                                                    >  2:2978569/1‑150 (MQ=255)
                                                                    aGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTaa                                                                                >  1:2988943/1‑150 (MQ=255)
                                                                    aGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTaa                                                                                >  2:4060567/1‑150 (MQ=255)
                                                                     gTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAAt                                                                               >  2:1651968/1‑150 (MQ=255)
                                                                       cTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGa                                                                             <  1:2932542/150‑1 (MQ=255)
                                                                           ggtgTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTg                                                                         >  1:4064461/1‑150 (MQ=255)
                                                                            gtgtGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGc                                                                        <  2:515710/150‑1 (MQ=255)
                                                                               tgCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTg                                                                     >  2:128278/1‑150 (MQ=255)
                                                                               tgCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTg                                                                     <  2:2467437/150‑1 (MQ=255)
                                                                                gCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTgg                                                                    <  2:188407/150‑1 (MQ=255)
                                                                                 cGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGc                                                                   >  1:3136666/1‑150 (MQ=255)
                                                                                      gCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGgaga                                                              >  1:1123063/1‑150 (MQ=255)
                                                                                       cTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGaa                                                             <  1:3170609/150‑1 (MQ=255)
                                                                                       cTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGaa                                                             <  1:3963777/150‑1 (MQ=255)
                                                                                           tgtTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTcc                                                         >  1:2072664/1‑150 (MQ=255)
                                                                                             tttttACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCAt                                                       <  2:2851726/150‑1 (MQ=255)
                                                                                             tttttACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCAt                                                       <  2:810167/150‑1 (MQ=255)
                                                                                              ttttACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATg                                                      <  1:1806024/150‑1 (MQ=255)
                                                                                                 tACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCg                                                   <  2:672955/150‑1 (MQ=255)
                                                                                                     ccGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATg                                               >  1:1113049/1‑150 (MQ=255)
                                                                                                     ccGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATg                                               >  2:3560262/1‑150 (MQ=255)
                                                                                                      cGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATgg                                              <  1:2133108/150‑1 (MQ=255)
                                                                                                        cATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCa                                            <  2:1451526/150‑1 (MQ=255)
                                                                                                              tGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTgcg                                      <  2:1335127/150‑1 (MQ=255)
                                                                                                              tGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTgcg                                      <  1:3647652/150‑1 (MQ=255)
                                                                                                                 gcTAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTa                                   <  2:2874592/150‑1 (MQ=255)
                                                                                                                   tAACCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATg                                 <  2:3584837/150‑1 (MQ=255)
                                                                                                                     aCCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCa                               >  1:3881148/1‑150 (MQ=255)
                                                                                                                     aCCTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCa                               <  2:445244/150‑1 (MQ=255)
                                                                                                                     aCCTTATGATCGGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCa                               <  2:2629638/150‑1 (MQ=255)
                                                                                                                       cTTATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAgg                             >  2:935461/1‑150 (MQ=255)
                                                                                                                        ttATGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGa                            <  1:1886245/150‑1 (MQ=255)
                                                                                                                           tGATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACAt                         <  2:3669654/150‑1 (MQ=255)
                                                                                                                            gATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATg                        >  1:2172816/1‑150 (MQ=255)
                                                                                                                            gATCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATg                        <  1:146086/150‑1 (MQ=255)
                                                                                                                             aTCTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGa                       >  2:1943/1‑150 (MQ=255)
                                                                                                                               cTGGCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGAtt                     >  2:373660/1‑150 (MQ=255)
                                                                                                                                 ggCAGACAACATGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGAtttt                   <  2:30151/150‑1 (MQ=255)
                                                                                                                                      acaacaTGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGt              <  1:1483203/150‑1 (MQ=255)
                                                                                                                                       caacaTGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTc             >  1:1647569/1‑150 (MQ=255)
                                                                                                                                         acaTGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCaa           >  1:1270471/1‑150 (MQ=255)
                                                                                                                                           aTGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAAt         >  1:2408121/1‑150 (MQ=255)
                                                                                                                                           aTGGGAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAAt         <  2:2570893/150‑1 (MQ=255)
                                                                                                                                              ggAGAAACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAAtgtg      <  1:3310136/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGA  >  CP009273/1801900‑1802195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑