Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,909,342 T→A 93.4% E150D (GAA→GAT)  proQ ← RNA chaperone, putative ProP translation regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,909,3420TA93.4% 835.9 / 35.8 242E150D (GAA→GAT) proQRNA chaperone, putative ProP translation regulator
Reads supporting (aligned to +/- strand):  ref base T (9/7);  new base A (111/115);  total (120/122)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 246 total aligned reads displayed.

GTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCT  >  CP009273/1909195‑1909490
                                                                                                                                                   |                                                                                                                                                    
gTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCcatc                                                                                                                                                    >  2:3577576/1‑150 (MQ=255)
  tttGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCcatctt                                                                                                                                                  <  1:2316801/150‑1 (MQ=255)
   ttGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCttt                                                                                                                                                 <  1:3506129/150‑1 (MQ=255)
   ttGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCttt                                                                                                                                                 <  2:3598241/150‑1 (MQ=255)
         cGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTgcggcg                                                                                                                                           >  2:873910/1‑150 (MQ=255)
         cGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTgcggcg                                                                                                                                           <  1:3774994/150‑1 (MQ=255)
         cGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTgcggcg                                                                                                                                           <  1:1585290/150‑1 (MQ=255)
             ttCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCggcgtggc                                                                                                                                       >  1:4155255/1‑150 (MQ=255)
               cACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCggcgtggcgt                                                                                                                                     >  2:77110/1‑150 (MQ=255)
               cACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCggcgtggcgt                                                                                                                                     >  2:430285/1‑150 (MQ=255)
               cACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCggcgtggcgt                                                                                                                                     >  2:2248193/1‑150 (MQ=255)
                aCCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTa                                                                                                                                    <  1:4184445/150‑1 (MQ=255)
                    tCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCg                                                                                                                                >  1:364509/1‑150 (MQ=255)
                     cAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCgg                                                                                                                               <  2:1037614/150‑1 (MQ=255)
                       gggCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGAc                                                                                                                             <  1:3827149/150‑1 (MQ=255)
                       gggCGTGTCCGACAGTCAGAGCTGAAATGTCAGATACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGAc                                                                                                                             <  2:4079015/150‑1 (MQ=255)
                        ggCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGATGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTTCGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACg                                                                                                                            <  2:3038712/150‑1 (MQ=255)
                        ggCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACg                                                                                                                            >  1:1746789/1‑150 (MQ=255)
                           ttGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTgg                                                                                                                         >  2:1752043/1‑150 (MQ=255)
                            tGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGc                                                                                                                        <  1:2509748/150‑1 (MQ=255)
                                 gACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCg                                                                                                                   <  2:2805499/150‑1 (MQ=255)
                                   cAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGtt                                                                                                                 <  1:693877/150‑1 (MQ=255)
                                   cAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGtt                                                                                                                 >  1:495587/1‑150 (MQ=255)
                                    aGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTc                                                                                                                <  1:200060/150‑1 (MQ=255)
                                       cAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcg                                                                                                             <  2:1963408/150‑1 (MQ=255)
                                        agagCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcgg                                                                                                            >  2:522595/1‑150 (MQ=255)
                                         gagCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggc                                                                                                           <  1:261253/150‑1 (MQ=255)
                                          agCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggcg                                                                                                          <  2:1181875/150‑1 (MQ=255)
                                              gAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGt                                                                                                      >  1:92387/1‑150 (MQ=255)
                                               aaaTGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTg                                                                                                     <  1:2556693/150‑1 (MQ=255)
                                                aaTGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGc                                                                                                    <  2:457226/150‑1 (MQ=255)
                                                aaTGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGc                                                                                                    <  2:2517634/150‑1 (MQ=255)
                                                  tGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGt                                                                                                  >  2:3480027/1‑150 (MQ=255)
                                                  tGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGt                                                                                                  >  1:2688735/1‑150 (MQ=255)
                                                    tCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGtct                                                                                                <  1:3943532/150‑1 (MQ=255)
                                                    tCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGtct                                                                                                <  1:628597/150‑1 (MQ=255)
                                                         aaCCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCttcttt                                                                                           >  2:4080868/1‑150 (MQ=255)
                                                               ggTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCAcc                                                                                     <  2:68537/150‑1 (MQ=255)
                                                               ggTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCAcc                                                                                     <  2:110007/150‑1 (MQ=255)
                                                                  gtgCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACcagc                                                                                  >  2:1632751/1‑150 (MQ=255)
                                                                    gCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACcagcag                                                                                >  1:3142007/1‑150 (MQ=255)
                                                                       gTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTg                                                                             >  2:1199664/1‑150 (MQ=255)
                                                                       gTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTg                                                                             >  2:1390041/1‑150 (MQ=255)
                                                                         tcttcGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCg                                                                           >  1:792677/1‑150 (MQ=255)
                                                                          cttcGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCgg                                                                          >  1:2939870/1‑150 (MQ=255)
                                                                          cttcGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGGTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCgg                                                                          >  2:733300/1‑150 (MQ=255)
                                                                             cgcgAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAg                                                                       <  2:1947287/150‑1 (MQ=255)
                                                                               cgAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCt                                                                     >  2:319553/1‑150 (MQ=255)
                                                                                gAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCtt                                                                    <  1:4202659/150‑1 (MQ=255)
                                                                                gAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCtt                                                                    >  2:1880934/1‑150 (MQ=255)
                                                                                gAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCtt                                                                    <  2:3867730/150‑1 (MQ=255)
                                                                                 aGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTc                                                                   <  1:2864409/150‑1 (MQ=255)
                                                                                 aGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTc                                                                   <  2:3604951/150‑1 (MQ=255)
                                                                                      cTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtt                                                              <  1:3063086/150‑1 (MQ=255)
                                                                                         ttACTTTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGttttt                                                           >  1:2778851/1‑150 (MQ=255)
                                                                                         ttACTTTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGttttt                                                           >  1:3227947/1‑150 (MQ=255)
                                                                                         ttACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGttttt                                                           <  2:4241869/150‑1 (MQ=255)
                                                                                          tACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttttt                                                          >  1:4019143/1‑150 (MQ=255)
                                                                                          tACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttttt                                                          >  2:4053914/1‑150 (MQ=255)
                                                                                          tACTGTTTTTGGCGCTGTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttttt                                                          <  1:2407634/150‑1 (MQ=255)
                                                                                           aCTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTc                                                         >  1:3338543/1‑150 (MQ=255)
                                                                                           aCTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTc                                                         >  1:1734724/1‑150 (MQ=255)
                                                                                             tGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGc                                                       >  2:394407/1‑150 (MQ=255)
                                                                                              gTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCt                                                      <  1:522595/150‑1 (MQ=255)
                                                                                               tttttGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCtt                                                     <  1:1458644/150‑1 (MQ=255)
                                                                                                  ttGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCt                                                  <  1:3355847/150‑1 (MQ=255)
                                                                                                   tGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTg                                                 >  1:1838685/1‑150 (MQ=255)
                                                                                                   tGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTg                                                 >  1:1804892/1‑150 (MQ=255)
                                                                                                   tGGAGCTTTCTCTACCGGCATTTGCGCACGACGTTTACGTACAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTTCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCTGTTGCGGCAGCTTCGCGTTTTTTCGCTTGATg                                                 >  2:4147496/1‑150 (MQ=255)
                                                                                                     gcgcATTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGtt                                               <  1:1054861/150‑1 (MQ=255)
                                                                                                      cgcTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTc                                              >  2:2163686/1‑150 (MQ=255)
                                                                                                      cgcTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTc                                              >  2:3610411/1‑150 (MQ=255)
                                                                                                         tttCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGc                                           >  1:14216/1‑150 (MQ=255)
                                                                                                           tctcTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCAc                                         <  2:3462089/150‑1 (MQ=255)
                                                                                                            ctctACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACg                                        <  1:2057157/150‑1 (MQ=255)
                                                                                                            ctctACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACg                                        <  1:4151766/150‑1 (MQ=255)
                                                                                                              ctACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCt                                      >  1:1254211/1‑150 (MQ=255)
                                                                                                              ctACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCt                                      <  1:1623380/150‑1 (MQ=255)
                                                                                                                aCCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCTCTTGCTGTTCAGCACGCtgt                                    >  1:2246559/1‑150 (MQ=255)
                                                                                                                  cGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGc                                  >  2:1530067/1‑150 (MQ=255)
                                                                                                                    gCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCt                                <  2:2668742/150‑1 (MQ=255)
                                                                                                                    gCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCt                                >  1:2866595/1‑150 (MQ=255)
                                                                                                                           cgcACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACAcg                         <  2:1480867/150‑1 (MQ=255)
                                                                                                                            gcACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACAcgc                        >  2:3389770/1‑150 (MQ=255)
                                                                                                                            gcACGAGGTTTACGTTAAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCATGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGATGCGGCAGCTACGCGTTTTTTCGCTTGCTGTTCAGCACGCGGTGCCTGAACAcgc                        >  2:111197/1‑150 (MQ=255)
                                                                                                                             cACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACAcgcg                       >  2:287686/1‑150 (MQ=255)
                                                                                                                                   gTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCg                 >  1:3928969/1‑150 (MQ=255)
                                                                                                                                   gTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTCCg                 <  2:2317177/150‑1 (MQ=255)
                                                                                                                                    tttACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGc                >  2:1847026/1‑150 (MQ=255)
                                                                                                                                     ttACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGct               >  1:3039117/1‑150 (MQ=255)
                                                                                                                                      tACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGctt              >  1:346827/1‑150 (MQ=255)
                                                                                                                                      tACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGctt              >  1:1155464/1‑150 (MQ=255)
                                                                                                                                       aCGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGcttc             <  1:830919/150‑1 (MQ=255)
                                                                                                                                       aCGTTCAGCGCCATCTGTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGcttc             <  1:555590/150‑1 (MQ=255)
                                                                                                                                          ttCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCttcttc          <  1:3840666/150‑1 (MQ=255)
                                                                                                                                            cAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCaa        <  2:4047773/150‑1 (MQ=255)
                                                                                                                                            cAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCaa        >  1:2654689/1‑150 (MQ=255)
                                                                                                                                               cgcCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAgct     <  2:1965537/150‑1 (MQ=255)
                                                                                                                                               cgcCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAgct     >  2:2088850/1‑150 (MQ=255)
                                                                                                                                                gcCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAgctg    >  1:3558186/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCT  >  CP009273/1909195‑1909490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑