Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,041,982 A→T 25.4% S1188S (TCA→TCT)  yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,041,9820AT25.4% 520.6 / 202.8 255S1188S (TCA→TCT) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base A (105/85);  new base T (32/32);  total (137/118)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 274 total aligned reads displayed.

TTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTG  >  CP009273/2041834‑2042129
                                                                                                                                                    |                                                                                                                                                   
ttGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATtcagctcag                                                                                                                                                    >  2:1030075/1‑150 (MQ=255)
  gtgGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGcc                                                                                                                                                  <  2:4334766/150‑1 (MQ=255)
      cGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCtt                                                                                                                                              <  2:3711643/150‑1 (MQ=255)
          cAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAgg                                                                                                                                          >  2:1586838/1‑150 (MQ=255)
          cAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAgg                                                                                                                                          >  1:3281645/1‑150 (MQ=255)
            aaGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGAc                                                                                                                                        >  2:3201765/1‑150 (MQ=255)
            aaGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGAc                                                                                                                                        <  1:2316596/150‑1 (MQ=255)
            aaGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGAc                                                                                                                                        <  2:2874938/150‑1 (MQ=255)
             aGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACt                                                                                                                                       >  2:2751408/1‑150 (MQ=255)
             aGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACt                                                                                                                                       <  1:923026/150‑1 (MQ=255)
             aGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACt                                                                                                                                       >  1:1853457/1‑150 (MQ=255)
               ccTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCa                                                                                                                                     <  1:2155480/150‑1 (MQ=255)
                cTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATCGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCAc                                                                                                                                    >  2:2079112/1‑150 (MQ=255)
                cTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCAc                                                                                                                                    <  1:2247070/150‑1 (MQ=255)
                    gCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTg                                                                                                                                >  2:1811215/1‑150 (MQ=255)
                        aGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGAccc                                                                                                                            >  1:2968177/1‑150 (MQ=255)
                           ttGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCgg                                                                                                                         >  2:1714481/1‑150 (MQ=255)
                            tGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCggg                                                                                                                        <  2:2238683/150‑1 (MQ=255)
                               ccTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGagt                                                                                                                     <  1:3986961/150‑1 (MQ=255)
                               ccTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGagt                                                                                                                     >  1:2652007/1‑150 (MQ=255)
                                cTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGagta                                                                                                                    >  1:1092308/1‑150 (MQ=255)
                                 tGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGagtaa                                                                                                                   <  1:1230610/150‑1 (MQ=255)
                                      atatCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAAt                                                                                                              >  2:96579/1‑150 (MQ=255)
                                      atatCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAAGAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAAt                                                                                                              <  1:1570970/150‑1 (MQ=255)
                                       tatCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATa                                                                                                             <  2:735907/150‑1 (MQ=255)
                                         tCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATAcc                                                                                                           >  1:2847069/1‑150 (MQ=255)
                                         tCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATAcc                                                                                                           >  1:677320/1‑150 (MQ=255)
                                           aaaaGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCaa                                                                                                         >  2:3940070/1‑150 (MQ=255)
                                            aaaGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAAc                                                                                                        <  2:3126615/150‑1 (MQ=255)
                                             aaGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACg                                                                                                       >  2:4221086/1‑150 (MQ=255)
                                              aGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGa                                                                                                      >  2:1266233/1‑150 (MQ=255)
                                              aGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGa                                                                                                      >  2:3340431/1‑150 (MQ=255)
                                                  gagaTCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCt                                                                                                  <  1:1621324/150‑1 (MQ=255)
                                                  gagaTCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCt                                                                                                  >  2:2447613/1‑150 (MQ=255)
                                                   agaTCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTg                                                                                                 <  2:1739502/150‑1 (MQ=255)
                                                    gaTCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTgg                                                                                                >  1:3509696/1‑150 (MQ=255)
                                                       cacaGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCAt                                                                                             >  2:3398427/1‑150 (MQ=255)
                                                        acaGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCGGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATc                                                                                            >  2:2609535/1‑150 (MQ=255)
                                                              aaTGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAg                                                                                      <  2:705662/150‑1 (MQ=255)
                                                               aTGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAgg                                                                                     >  2:626345/1‑150 (MQ=255)
                                                                   cGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCAc                                                                                 >  2:1683298/1‑150 (MQ=255)
                                                                   cGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCAc                                                                                 <  1:51078/150‑1 (MQ=255)
                                                                    gTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCAct                                                                                >  2:2553180/1‑150 (MQ=255)
                                                                       gATAGCGCAACGCTAACTGCAACGGTTAAAGATAAGTTCGACAATGAGGTGAATAATCTTCCGGTAAAATTCAGCTCTGCCTCTTCAGGACTCACCCTGACACCGCGAGTAAGTAATACCAACCAGACTGGCATCGCGCAGGAAACtctc                                                                             >  1:1101526/1‑150 (MQ=255)
                                                                         tAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGc                                                                           >  2:2580923/1‑150 (MQ=255)
                                                                         tAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGc                                                                           >  2:1803299/1‑150 (MQ=255)
                                                                          aGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAAACTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCa                                                                          <  1:54759/150‑1 (MQ=255)
                                                                             gcAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGc                                                                       >  2:3315756/1‑150 (MQ=255)
                                                                             gcAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGc                                                                       >  1:197186/1‑150 (MQ=255)
                                                                              cAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCg                                                                      >  2:1879539/1‑150 (MQ=255)
                                                                               aaCGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGt                                                                     >  1:2890989/1‑150 (MQ=255)
                                                                                 cGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGCGGTGACTAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTg                                                                   <  1:4055495/150‑1 (MQ=255)
                                                                                    tAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCGGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCt                                                                <  2:473344/150‑1 (MQ=255)
                                                                                    tAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCt                                                                >  1:1958004/1‑150 (MQ=255)
                                                                                     aaCTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCtt                                                               <  1:3749274/150‑1 (MQ=255)
                                                                                     aaCTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCtt                                                               <  2:1163512/150‑1 (MQ=255)
                                                                                      aCTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCttt                                                              <  2:1504284/150‑1 (MQ=255)
                                                                                      aCTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCttt                                                              <  1:1268212/150‑1 (MQ=255)
                                                                                       cTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTg                                                             <  2:872591/150‑1 (MQ=255)
                                                                                        tGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTgg                                                            >  2:2111271/1‑150 (MQ=255)
                                                                                          cAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTCACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTGGGGTg                                                          >  1:3377729/1‑150 (MQ=255)
                                                                                          cAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTg                                                          >  1:3027664/1‑150 (MQ=255)
                                                                                            aCGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTgag                                                        <  2:1665475/150‑1 (MQ=255)
                                                                                             cGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTgaga                                                       >  2:2898741/1‑150 (MQ=255)
                                                                                              ggTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCATTGGTGagaa                                                      >  1:4209771/1‑150 (MQ=255)
                                                                                               gTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGagaag                                                     <  1:4303825/150‑1 (MQ=255)
                                                                                               gTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGagaag                                                     <  1:4227136/150‑1 (MQ=255)
                                                                                               gTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGagaag                                                     >  2:4052345/1‑150 (MQ=255)
                                                                                               gTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGagaag                                                     >  2:1862206/1‑150 (MQ=255)
                                                                                                ttAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGagaaga                                                    >  1:2351287/1‑150 (MQ=255)
                                                                                                 tAAAGATCAGTTCGACAATGAGGTGACTAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCCCCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGAc                                                   <  2:1182324/150‑1 (MQ=255)
                                                                                                 tAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGAc                                                   <  2:1391308/150‑1 (MQ=255)
                                                                                                    aGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGt                                                >  2:3478208/1‑150 (MQ=255)
                                                                                                        cAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACt                                            >  2:1934994/1‑150 (MQ=255)
                                                                                                          gTTCGACAATGAGGTGAATACTCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGc                                          <  1:2692748/150‑1 (MQ=255)
                                                                                                              gACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCa                                      <  2:2652007/150‑1 (MQ=255)
                                                                                                               aCAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCAc                                     >  1:3065841/1‑150 (MQ=255)
                                                                                                                cAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACt                                    <  2:2271640/150‑1 (MQ=255)
                                                                                                                  aTGAGGTGAATCATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTgg                                  <  1:3640348/150‑1 (MQ=255)
                                                                                                                  aTGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTgg                                  >  1:4142092/1‑150 (MQ=255)
                                                                                                                  aTGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAACACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTgg                                  <  2:2075743/150‑1 (MQ=255)
                                                                                                                     aGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCta                               >  2:2791108/1‑150 (MQ=255)
                                                                                                                      ggTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCtaa                              >  1:3949483/1‑150 (MQ=255)
                                                                                                                       gTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCGGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCtaat                             >  1:4000463/1‑150 (MQ=255)
                                                                                                                           ataatCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGTCGGTTACTGCATCACTGGCTAATAATg                         >  1:1265949/1‑150 (MQ=255)
                                                                                                                             aatCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGt                       >  1:4293541/1‑150 (MQ=255)
                                                                                                                              atCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTg                      <  1:1811215/150‑1 (MQ=255)
                                                                                                                                cTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGcc                    <  2:3821102/150‑1 (MQ=255)
                                                                                                                                cTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGcc                    <  2:1632204/150‑1 (MQ=255)
                                                                                                                                 ttCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCa                   <  2:2020947/150‑1 (MQ=255)
                                                                                                                                 ttCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCa                   <  1:2019000/150‑1 (MQ=255)
                                                                                                                                  tCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAg                  <  1:1683298/150‑1 (MQ=255)
                                                                                                                                       tAACATTCGGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGaca             >  2:2989063/1‑150 (MQ=255)
                                                                                                                                        aaCATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGacaa            <  2:1361805/150‑1 (MQ=255)
                                                                                                                                              cagctcagcCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAAc      >  2:3571910/1‑150 (MQ=255)
                                                                                                                                              cagctcagcCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAAc      <  2:4279034/150‑1 (MQ=255)
                                                                                                                                               agctcagcCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACt     <  2:2968177/150‑1 (MQ=255)
                                                                                                                                               agctcagcCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACt     >  2:4181849/1‑150 (MQ=255)
                                                                                                                                               agctcagcCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACt     >  2:2998234/1‑150 (MQ=255)
                                                                                                                                                  tcagcCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACtgtg  >  2:1818562/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTG  >  CP009273/2041834‑2042129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑