Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,362,338 T→A 100% L274Q (CTG→CAG)  arnA → fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,362,3380TA96.1% 992.8 / 22.0 279L274Q (CTG→CAG) arnAfused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (7/4);  new base A (153/115);  total (160/119)
Fisher's exact test for biased strand distribution p-value = 7.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 279 total aligned reads displayed.

CCGATCCGTGGCCGGGTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCTGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCTCGGGG  >  CP009273/2362191‑2362484
                                                                                                                                                   |                                                                                                                                                  
     ccGTGGCCGGGTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATc                                                                                                                                             >  2:1075276/1‑150 (MQ=255)
           ccGGGTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCAcc                                                                                                                                       >  1:3860177/1‑150 (MQ=255)
           ccGGGTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCAcc                                                                                                                                       <  2:1241327/150‑1 (MQ=255)
              ggTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGa                                                                                                                                    >  2:626611/1‑150 (MQ=255)
               gTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGAc                                                                                                                                   >  1:1281465/1‑150 (MQ=255)
                   cTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAggc                                                                                                                               >  2:1562045/1‑150 (MQ=255)
                       aGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAggcgggc                                                                                                                           <  1:3139824/150‑1 (MQ=255)
                       aGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAggcgggc                                                                                                                           >  2:296464/1‑150 (MQ=255)
                         cTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGa                                                                                                                         >  1:2455593/1‑150 (MQ=255)
                         cTATGTTGGCAATCAGAAATTCACCGTCTGGACGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGa                                                                                                                         >  2:1019930/1‑150 (MQ=255)
                          tATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGAc                                                                                                                        >  1:1618572/1‑150 (MQ=255)
                           aTGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACg                                                                                                                       >  2:2380352/1‑150 (MQ=255)
                           aTGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACg                                                                                                                       >  2:206921/1‑150 (MQ=255)
                           aTGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACg                                                                                                                       >  2:3761464/1‑150 (MQ=255)
                           aTGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACg                                                                                                                       >  1:3186646/1‑150 (MQ=255)
                             gTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGc                                                                                                                     >  2:3469312/1‑150 (MQ=255)
                               tGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCAt                                                                                                                   <  2:3394377/150‑1 (MQ=255)
                               tGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGAGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCAt                                                                                                                   >  1:3525732/1‑150 (MQ=255)
                                ggCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCAtt                                                                                                                  >  1:1701000/1‑150 (MQ=255)
                                 gCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTa                                                                                                                 <  2:313663/150‑1 (MQ=255)
                                  cAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTAc                                                                                                                >  2:2181104/1‑150 (MQ=255)
                                    ctCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTa                                                                                                              <  2:791729/149‑1 (MQ=255)
                                     tCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCTGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTAt                                                                                                             <  2:283184/150‑1 (MQ=255)
                                     tCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTAt                                                                                                             <  2:1742345/150‑1 (MQ=255)
                                        gAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCa                                                                                                          >  1:1046556/1‑150 (MQ=255)
                                        gAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCa                                                                                                          >  1:1047613/1‑150 (MQ=255)
                                         aaaTTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAg                                                                                                         >  2:3495919/1‑150 (MQ=255)
                                          aaTTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCAACGGCATTACTATGCAgg                                                                                                        >  1:2011373/1‑150 (MQ=255)
                                             tCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCt                                                                                                     <  1:230409/150‑1 (MQ=255)
                                             tCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCt                                                                                                     <  1:3884992/150‑1 (MQ=255)
                                                 cGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCa                                                                                                 <  1:3115789/150‑1 (MQ=255)
                                                    cTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAAtt                                                                                              >  1:3011634/1‑150 (MQ=255)
                                                    cTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAAtt                                                                                              >  1:1217172/1‑150 (MQ=255)
                                                     tGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTa                                                                                             >  2:2531413/1‑150 (MQ=255)
                                                     tGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTa                                                                                             >  1:560077/1‑150 (MQ=255)
                                                      ggTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAg                                                                                            <  1:2129665/150‑1 (MQ=255)
                                                       gtcgtcGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAgc                                                                                           <  1:439289/150‑1 (MQ=255)
                                                        tcgtcgCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCTGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAgcg                                                                                          <  2:681798/150‑1 (MQ=255)
                                                          gtcgCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCa                                                                                        >  2:3629551/1‑150 (MQ=255)
                                                          gtcgCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCa                                                                                        >  2:2221586/1‑150 (MQ=255)
                                                          gtcgCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCAGTACTATGCAGGGCTCGCACTTAGCGCa                                                                                        >  2:3210250/1‑150 (MQ=255)
                                                           tcgCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAg                                                                                       <  1:4186123/150‑1 (MQ=255)
                                                            cgcgTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGa                                                                                      >  2:3982485/1‑150 (MQ=255)
                                                               gtgtTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGc                                                                                   <  2:1696426/150‑1 (MQ=255)
                                                                tgtTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCt                                                                                  >  1:366827/1‑150 (MQ=255)
                                                                 gtTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTg                                                                                 >  1:3650934/1‑150 (MQ=255)
                                                                 gtTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTg                                                                                 >  1:1751556/1‑150 (MQ=255)
                                                                 gtTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTg                                                                                 <  2:4320136/150‑1 (MQ=255)
                                                                  ttCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTgg                                                                                <  1:3661221/150‑1 (MQ=255)
                                                                   tCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTggg                                                                               >  2:3378487/1‑150 (MQ=255)
                                                                         tCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCCGACGCTGGGCCTGGt                                                                         >  1:2506588/1‑150 (MQ=255)
                                                                         tCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGt                                                                         <  1:4122306/150‑1 (MQ=255)
                                                                         tCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGt                                                                         >  1:332629/1‑150 (MQ=255)
                                                                              ccAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAg                                                                    >  2:2773094/1‑150 (MQ=255)
                                                                                   aaaGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCa                                                               <  1:1341343/150‑1 (MQ=255)
                                                                                   aaaGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCa                                                               >  2:1170481/1‑150 (MQ=255)
                                                                                   aaaGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCa                                                               >  2:2713469/1‑150 (MQ=255)
                                                                                    aaGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCAc                                                              >  2:575031/1‑150 (MQ=255)
                                                                                    aaGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCAc                                                              >  2:2868297/1‑150 (MQ=255)
                                                                                      gCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCTGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGc                                                            >  2:2582961/1‑150 (MQ=255)
                                                                                        acaGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCtt                                                          >  1:160696/1‑150 (MQ=255)
                                                                                         caGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTg                                                         >  2:3431896/1‑150 (MQ=255)
                                                                                            ccGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAAt                                                      <  1:285526/150‑1 (MQ=255)
                                                                                            ccGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAAt                                                      <  1:4078131/150‑1 (MQ=255)
                                                                                            ccGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAAt                                                      <  2:1906893/150‑1 (MQ=255)
                                                                                             cGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATa                                                     >  1:194909/1‑150 (MQ=255)
                                                                                             cGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATa                                                     <  2:3868613/150‑1 (MQ=255)
                                                                                              ggggAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATag                                                    >  2:740708/1‑150 (MQ=255)
                                                                                              ggggAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATag                                                    >  1:4000638/1‑150 (MQ=255)
                                                                                               gggAGCGTGATTTCTGTTGCGCCACTGCTGATTGACTGTGGCGAAGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGAGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATaga                                                   >  1:2065121/1‑149 (MQ=255)
                                                                                                ggAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATagcc                                                  <  2:1913908/150‑1 (MQ=255)
                                                                                                 gAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATagcca                                                 <  2:2569823/150‑1 (MQ=255)
                                                                                                     gTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcct                                             <  2:3704179/150‑1 (MQ=255)
                                                                                                     gTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcct                                             <  2:2139640/150‑1 (MQ=255)
                                                                                                      tGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctg                                            >  2:1192106/1‑150 (MQ=255)
                                                                                                      tGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctg                                            >  1:3035883/1‑150 (MQ=255)
                                                                                                      tGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctg                                            >  2:4090483/1‑150 (MQ=255)
                                                                                                      tGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctg                                            >  1:2657536/1‑150 (MQ=255)
                                                                                                      tGATTTCTGTTGCGCCACTGCTGATGGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctg                                            <  2:1856196/150‑1 (MQ=255)
                                                                                                        aTTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctgcc                                          >  2:2460688/1‑150 (MQ=255)
                                                                                                         tttCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctgcct                                         >  1:938906/1‑150 (MQ=255)
                                                                                                             tGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCAc                                     >  1:782673/1‑150 (MQ=255)
                                                                                                              gTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCAcc                                    <  2:1786770/150‑1 (MQ=255)
                                                                                                                      aCTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACg                            >  2:2225410/1‑150 (MQ=255)
                                                                                                                      aCTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACg                            <  1:1456748/150‑1 (MQ=255)
                                                                                                                        tgctgATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGcc                          <  1:2593472/150‑1 (MQ=255)
                                                                                                                           tgATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTa                       >  2:3513358/1‑150 (MQ=255)
                                                                                                                           tgATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTa                       <  1:2432178/150‑1 (MQ=255)
                                                                                                                              ttGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTAccc                    <  1:626611/150‑1 (MQ=255)
                                                                                                                                 ccTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCggg                 <  2:3080996/150‑1 (MQ=255)
                                                                                                                                       gCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCa           <  2:1343768/150‑1 (MQ=255)
                                                                                                                                        cGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCAt          <  1:1125952/150‑1 (MQ=255)
                                                                                                                                         gATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATc         <  2:2627331/150‑1 (MQ=255)
                                                                                                                                         gATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATc         >  1:373248/1‑150 (MQ=255)
                                                                                                                                          aTGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATcc        <  1:4249633/150‑1 (MQ=255)
                                                                                                                                           tGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCt       >  1:151751/1‑150 (MQ=255)
                                                                                                                                            ggCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCTc      <  2:1096625/150‑1 (MQ=255)
                                                                                                                                              cgcgcAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCTCgg    <  1:4270849/150‑1 (MQ=255)
                                                                                                                                                cgcAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCTCgggg  <  1:3503986/150‑1 (MQ=255)
                                                                                                                                                cgcAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCTCgggg  <  1:3293923/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCGATCCGTGGCCGGGTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCTGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCTCGGGG  >  CP009273/2362191‑2362484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑