BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,412,205	C→A	46.5%	D151Y (GAT→TAT)	yfcD ←	putative NUDIX hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,412,205	0	C	A	46.5%	163.7 / inf	294	D151Y (GAT→TAT)	yfcD	putative NUDIX hydrolase
Reads supporting (aligned to +/- strand): ref base C (94/63); new base A (81/56); total (175/119)
Fisher's exact test for biased strand distribution p-value = 9.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 298 total aligned reads displayed.

CGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCT  >  CP009273/2412056‑2412351

cgcTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATc                                                                                                                                                    <  2:10394/150‑1 (MQ=255)

     aGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATagcagc                                                                                                                                               <  2:2693840/150‑1 (MQ=255)

     aGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGc                                                                                                                                               >  2:1302544/1‑150 (MQ=255)

     aGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGc                                                                                                                                               >  1:3939218/1‑150 (MQ=255)

      gTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCg                                                                                                                                              <  1:2981721/150‑1 (MQ=255)

        tGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTg                                                                                                                                            >  2:3760359/1‑150 (MQ=255)

         gATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGc                                                                                                                                           >  1:2041711/1‑150 (MQ=255)

          aTGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCg                                                                                                                                          >  1:2131470/1‑150 (MQ=255)

          aTGGGCTACCACTAAAAATGTAAAGAGCCGCAATTGCCGCTCATTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCg                                                                                                                                          <  1:17401/150‑1 (MQ=255)

           tGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCgg                                                                                                                                         >  2:1896301/1‑150 (MQ=255)

           tGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCgg                                                                                                                                         <  1:3601310/150‑1 (MQ=255)

                 aCCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGAttt                                                                                                                                   <  1:2938037/150‑1 (MQ=255)

                 aCCACTAAAAATGTTAAGAGCCGCAAGTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGAttt                                                                                                                                   <  1:2394808/150‑1 (MQ=255)

                   cACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATttct                                                                                                                                 <  2:744805/150‑1 (MQ=255)

                       aaaaaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCg                                                                                                                             >  2:241128/1‑150 (MQ=255)

                       aaaaaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATttcttcag                                                                                                                             >  1:209151/1‑148 (MQ=255)

                        aaaaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCgg                                                                                                                            >  1:935895/1‑150 (MQ=255)

                         aaaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGAGCGGTGATTTCTTCCGGc                                                                                                                           >  2:1418960/1‑150 (MQ=255)

                           aTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCAACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTATCGCTTTAAGCGAGTCTGGAGTGAACTCATATCAGCGTGCGGGGTTTTATTGCGGCGt                                                                                                                         >  1:769071/1‑150 (MQ=255)

                                 agagCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTcagcca                                                                                                                   >  2:1949076/1‑150 (MQ=255)

                                  gagCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTcagccag                                                                                                                  <  1:543831/150‑1 (MQ=255)

                                  gagCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTcagccag                                                                                                                  <  1:1119504/150‑1 (MQ=255)

                                      cGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCaaa                                                                                                              >  1:916749/1‑150 (MQ=255)

                                       gCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAAc                                                                                                             <  1:2018107/150‑1 (MQ=255)

                                        cAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACt                                                                                                            >  2:2154085/1‑150 (MQ=255)

                                        cAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACt                                                                                                            >  2:1515173/1‑150 (MQ=255)

                                          aTTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTc                                                                                                          <  2:723485/150‑1 (MQ=255)

                                             gCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACt                                                                                                       <  1:3443923/150‑1 (MQ=255)

                                              cGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTg                                                                                                      <  1:336598/150‑1 (MQ=255)

                                              cGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTg                                                                                                      <  1:3402014/150‑1 (MQ=255)

                                              cGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATACACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCAACGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTg                                                                                                      >  1:3200672/1‑150 (MQ=255)

                                               ggCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCGTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGa                                                                                                     >  1:3632267/1‑150 (MQ=255)

                                                gCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGAc                                                                                                    <  1:652771/150‑1 (MQ=255)

                                                gCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGAc                                                                                                    >  2:1711223/1‑150 (MQ=255)

                                                gCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTATTCCGGCGTCAGCCAGCAAACTTCACTGAc                                                                                                    >  1:3433480/1‑150 (MQ=255)

                                                     tttttCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCAt                                                                                               <  1:2627204/150‑1 (MQ=255)

                                                     tttttCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCAt                                                                                               >  1:3195848/1‑150 (MQ=255)

                                                        ttCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCtt                                                                                            <  1:2556032/150‑1 (MQ=255)

                                                        ttCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCtt                                                                                            <  1:2436574/150‑1 (MQ=255)

                                                          cATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTcc                                                                                          >  2:3206306/1‑150 (MQ=255)

                                                           aTTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCt                                                                                         >  1:1396752/1‑150 (MQ=255)

                                                           aTTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCt                                                                                         >  2:3011705/1‑150 (MQ=255)

                                                              cTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTa                                                                                      >  2:2577909/1‑150 (MQ=255)

                                                              cTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTa                                                                                      <  1:4188092/150‑1 (MQ=255)

                                                                 ccGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTg                                                                                   >  1:2769666/1‑150 (MQ=255)

                                                                   gTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCg                                                                                 >  2:3712577/1‑150 (MQ=255)

                                                                     ttCAGTATATTCGGCTTCATTTTTGTCATTGCGCTTCATCCACAACGCTAGCGCTTTGAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGaa                                                                               <  1:3883579/150‑1 (MQ=255)

                                                                       cAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAgg                                                                             >  1:716529/1‑150 (MQ=255)

                                                                       cAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAgg                                                                             >  2:816642/1‑150 (MQ=255)

                                                                         gTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGa                                                                           >  1:4014526/1‑150 (MQ=255)

                                                                           ctctACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGAGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGAGAAGGGAcc                                                                         >  2:3827850/1‑150 (MQ=255)

                                                                              tACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTg                                                                      <  1:2219450/150‑1 (MQ=255)

                                                                              tACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTg                                                                      >  2:3525500/1‑150 (MQ=255)

                                                                               aCGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTga                                                                     >  1:3529993/1‑150 (MQ=255)

                                                                                cGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTgag                                                                    <  1:673377/150‑1 (MQ=255)

                                                                                  gCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTgagag                                                                  <  1:2699777/150‑1 (MQ=255)

                                                                                       aTTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCa                                                             >  1:789918/1‑150 (MQ=255)

                                                                                        tttttGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAg                                                            <  2:916749/150‑1 (MQ=255)

                                                                                         ttttGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGTGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGc                                                           >  2:686598/1‑150 (MQ=255)

                                                                                             ggCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGaa                                                       <  1:4334722/150‑1 (MQ=255)

                                                                                                 ttGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAAt                                                   <  1:2674699/150‑1 (MQ=255)

                                                                                                 ttGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAAt                                                   <  1:1185959/150‑1 (MQ=255)

                                                                                                   gcgcTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATgc                                                 <  2:2029874/150‑1 (MQ=255)

                                                                                                    cgcTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATgcg                                                >  2:3231795/1‑150 (MQ=255)

                                                                                                    cgcTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATgcg                                                <  2:2858993/150‑1 (MQ=255)

                                                                                                       ttCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGccc                                             >  1:3794934/1‑150 (MQ=255)

                                                                                                        tCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGcccc                                            <  2:1168330/150‑1 (MQ=255)

                                                                                                        tCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGcccc                                            >  2:3952075/1‑150 (MQ=255)

                                                                                                         cATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCa                                           >  2:3168226/1‑150 (MQ=255)

                                                                                                         cATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCa                                           >  2:3301057/1‑150 (MQ=255)

                                                                                                         cATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCa                                           <  2:4273962/150‑1 (MQ=255)

                                                                                                              acaACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGacac                                      <  2:3062799/150‑1 (MQ=255)

                                                                                                                aaCGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACgg                                    >  2:1100508/1‑150 (MQ=255)

                                                                                                                aaCGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACgg                                    >  1:1786113/1‑150 (MQ=255)

                                                                                                                aaCGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACgg                                    >  2:2813064/1‑150 (MQ=255)

                                                                                                                  cGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCa                                  <  2:1240092/150‑1 (MQ=255)

                                                                                                                   gCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCaa                                 >  1:3244997/1‑150 (MQ=255)

                                                                                                                    cTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAt                                >  1:889534/1‑150 (MQ=255)

                                                                                                                    cTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAt                                >  1:3070532/1‑150 (MQ=255)

                                                                                                                     tAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAtt                               <  2:176789/150‑1 (MQ=255)

                                                                                                                      aGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAttt                              >  2:3687093/1‑150 (MQ=255)

                                                                                                                        cgcTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCACttttt                            >  2:1058005/1‑150 (MQ=255)

                                                                                                                         gcTTTCAGCGAGTCTGGAGTGAACTAATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGAAGCTGAACAATGCGCCCCAGACACGGCAATTTTTa                           >  1:1882913/1‑150 (MQ=255)

                                                                                                                          cTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGTCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTAt                          <  1:1654829/150‑1 (MQ=255)

                                                                                                                            ttCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCt                        <  2:539604/150‑1 (MQ=255)

                                                                                                                             tCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCtt                       <  1:4051394/150‑1 (MQ=255)

                                                                                                                               aGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCg                     <  2:2430865/150‑1 (MQ=255)

                                                                                                                               aGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCg                     <  1:1700014/150‑1 (MQ=255)

                                                                                                                                    gTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATa                <  1:2802119/150‑1 (MQ=255)

                                                                                                                                    gTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATa                >  1:2174491/1‑150 (MQ=255)

                                                                                                                                     tCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAg               >  2:2103043/1‑150 (MQ=255)

                                                                                                                                      cTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGa              <  2:1663635/150‑1 (MQ=255)

                                                                                                                                         gAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACt           <  1:3861912/150‑1 (MQ=255)

                                                                                                                                          aGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTg          <  2:3939218/150‑1 (MQ=255)

                                                                                                                                           gTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGc         >  2:27817/1‑150 (MQ=255)

                                                                                                                                             gAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAACTAGAACTGccc       >  2:3456015/1‑150 (MQ=255)

                                                                                                                                              aaCTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCg      <  2:1203974/150‑1 (MQ=255)

                                                                                                                                                cTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTg    <  1:1535414/150‑1 (MQ=255)

                                                                                                                                                cTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTg    <  1:3746683/150‑1 (MQ=255)

                                                                                                                                                  cATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCt  >  1:1275405/1‑150 (MQ=255)

CGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCT  >  CP009273/2412056‑2412351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`