BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,516,553	T→G	20.5%	E264D (GAA→GAC)	xapB ←	xanthosine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,516,553	0	T	G	20.5%	552.7 / 186.5	279	E264D (GAA→GAC)	xapB	xanthosine transporter
Reads supporting (aligned to +/- strand): ref base T (121/101); new base G (33/24); total (154/125)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01

Only 100 of 284 total aligned reads displayed.

ACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAG  >  CP009273/2516405‑2516702

 cagcagcagCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTc                                                                                                                                                     >  2:3428073/1‑150 (MQ=255)

  agcagcagcAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCt                                                                                                                                                    >  1:838526/1‑150 (MQ=255)

   gcagcagcaAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTg                                                                                                                                                   >  2:1318354/1‑150 (MQ=255)

   gcagcagcaAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTg                                                                                                                                                   >  2:1985447/1‑150 (MQ=255)

   gcagcagcaAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTg                                                                                                                                                   >  2:857816/1‑150 (MQ=255)

     agcagcaAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGcc                                                                                                                                                 >  2:855390/1‑150 (MQ=255)

     agcagcaAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGcc                                                                                                                                                 <  1:1294759/150‑1 (MQ=255)

      gcagcaAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCa                                                                                                                                                >  1:3375443/1‑150 (MQ=255)

       cagcaAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCAt                                                                                                                                               <  2:2117492/150‑1 (MQ=255)

        agcaAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATc                                                                                                                                              >  1:3924025/1‑150 (MQ=255)

             aataaatCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGa                                                                                                                                         <  1:3348417/150‑1 (MQ=255)

               taaatCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGaaa                                                                                                                                       >  1:990742/1‑150 (MQ=255)

                 aatCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACt                                                                                                                                     >  1:4308546/1‑150 (MQ=255)

                 aatCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACt                                                                                                                                     >  1:3404488/1‑150 (MQ=255)

                 aatCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACt                                                                                                                                     <  2:2753803/150‑1 (MQ=255)

                  atCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTg                                                                                                                                    >  1:3832492/1‑150 (MQ=255)

                      ggTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAg                                                                                                                                <  2:2952230/150‑1 (MQ=255)

                         tgttgACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTaa                                                                                                                             <  2:1051608/150‑1 (MQ=255)

                         tgttgACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTaa                                                                                                                             >  1:2206041/1‑150 (MQ=255)

                         tgttgACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTaa                                                                                                                             >  1:29855/1‑150 (MQ=255)

                             gACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATg                                                                                                                         >  2:1798945/1‑150 (MQ=255)

                              aCGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATgg                                                                                                                        <  2:2239829/150‑1 (MQ=255)

                                ggATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAg                                                                                                                      <  1:665530/150‑1 (MQ=255)

                                ggATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAg                                                                                                                      >  2:2163232/1‑150 (MQ=255)

                                 gATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAgg                                                                                                                     <  2:4318958/150‑1 (MQ=255)

                                   tCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGa                                                                                                                   <  2:1284738/150‑1 (MQ=255)

                                   tCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGa                                                                                                                   <  1:514429/150‑1 (MQ=255)

                                   tCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGa                                                                                                                   >  1:1666741/1‑150 (MQ=255)

                                       cATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATAtt                                                                                                               <  2:2887863/150‑1 (MQ=255)

                                              gaagaaGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTcaccac                                                                                                        <  2:4212495/150‑1 (MQ=255)

                                                    gCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACt                                                                                                  <  2:1677604/150‑1 (MQ=255)

                                                     ccAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTg                                                                                                 <  1:857896/150‑1 (MQ=255)

                                                        aaGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCa                                                                                              >  2:1035412/1‑150 (MQ=255)

                                                        aaGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCa                                                                                              >  2:2874417/1‑150 (MQ=255)

                                                         aGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAg                                                                                             >  1:1051181/1‑150 (MQ=255)

                                                         aGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAg                                                                                             >  2:2575376/1‑150 (MQ=255)

                                                             cAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCaaa                                                                                         <  1:2775945/150‑1 (MQ=255)

                                                                cGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTc                                                                                      >  2:1646730/1‑150 (MQ=255)

                                                                cGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTc                                                                                      >  1:1833457/1‑150 (MQ=255)

                                                                cGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTc                                                                                      >  2:3828607/1‑150 (MQ=255)

                                                                 gTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTcc                                                                                     >  2:18478/1‑150 (MQ=255)

                                                                  tCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCg                                                                                    >  1:438484/1‑150 (MQ=255)

                                                                      ggCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGtt                                                                                <  1:3105674/150‑1 (MQ=255)

                                                                         caccaTACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACg                                                                             <  1:2662529/150‑1 (MQ=255)

                                                                             ctACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCg                                                                         <  2:3630672/149‑1 (MQ=255)

                                                                                cTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGaaa                                                                      >  2:2357360/1‑150 (MQ=255)

                                                                                  catcaGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATc                                                                    <  2:1427302/150‑1 (MQ=255)

                                                                                     caGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATg                                                                 <  1:2322162/150‑1 (MQ=255)

                                                                                       gCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTa                                                               <  1:2891368/150‑1 (MQ=255)

                                                                                        cATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAg                                                              >  1:1410412/1‑150 (MQ=255)

                                                                                         aTGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAgg                                                             >  1:715605/1‑150 (MQ=255)

                                                                                         aTGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAgg                                                             <  1:2485096/150‑1 (MQ=255)

                                                                                          tGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGa                                                            >  2:3356744/1‑150 (MQ=255)

                                                                                             cGGTTTTAATGCCAAATCGATTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACg                                                         <  2:443505/150‑1 (MQ=255)

                                                                                              ggTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACgg                                                        <  1:2353633/150‑1 (MQ=255)

                                                                                               gTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGa                                                       >  1:3510314/1‑150 (MQ=255)

                                                                                               gTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGa                                                       >  2:1145987/1‑150 (MQ=255)

                                                                                                ttttAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGAt                                                      >  2:938843/1‑150 (MQ=255)

                                                                                                 tttAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGAtt                                                     >  2:3246842/1‑150 (MQ=255)

                                                                                                  ttAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTa                                                    <  1:3068755/150‑1 (MQ=255)

                                                                                                  ttAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTa                                                    <  1:3317871/150‑1 (MQ=255)

                                                                                                   tAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTAc                                                   <  2:11744/150‑1 (MQ=255)

                                                                                                   tAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTAc                                                   >  1:928707/1‑150 (MQ=255)

                                                                                                     aTGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCa                                                 >  1:54740/1‑150 (MQ=255)

                                                                                                        ccAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCaaaa                                              >  2:642797/1‑150 (MQ=255)

                                                                                                        ccAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCaaaa                                              >  1:4292846/1‑150 (MQ=255)

                                                                                                         cAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCaaaaa                                             >  2:3854843/1‑150 (MQ=255)

                                                                                                            aTCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGt                                          >  1:4334928/1‑150 (MQ=255)

                                                                                                            aTCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGt                                          >  1:3979945/1‑150 (MQ=255)

                                                                                                             tCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGtt                                         <  1:4262532/150‑1 (MQ=255)

                                                                                                               gCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTgg                                       >  2:1589383/1‑150 (MQ=255)

                                                                                                               gCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTgg                                       >  1:2740739/1‑150 (MQ=255)

                                                                                                               gCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTgg                                       >  1:2216188/1‑150 (MQ=255)

                                                                                                                cTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACATTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGt                                      <  1:786744/150‑1 (MQ=255)

                                                                                                                cTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGt                                      >  1:4290630/1‑150 (MQ=255)

                                                                                                                  ttAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTaa                                    >  1:3460993/1‑150 (MQ=255)

                                                                                                                    aaaaaGAATGGGATAGTCAGTATAAATCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAAtt                                  >  2:3756430/1‑150 (MQ=255)

                                                                                                                      aaaGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTg                                <  2:1431866/150‑1 (MQ=255)

                                                                                                                      aaaGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATAATGTAGGAACGGATTACCAAAAACGTTGGTAATTTg                                >  2:54636/1‑150 (MQ=255)

                                                                                                                       aaGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGc                               <  2:3924025/150‑1 (MQ=255)

                                                                                                                        aGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCa                              <  2:3404488/150‑1 (MQ=255)

                                                                                                                        aGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCa                              <  1:3031566/150‑1 (MQ=255)

                                                                                                                         gAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAg                             >  1:1958625/1‑150 (MQ=255)

                                                                                                                         gAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAg                             <  1:325977/150‑1 (MQ=255)

                                                                                                                           aTGGGATAGTCAGTATAACGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTa                           <  2:3973846/150‑1 (MQ=255)

                                                                                                                           aTGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTa                           <  2:3135885/150‑1 (MQ=255)

                                                                                                                            tGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTAc                          >  2:1309617/1‑150 (MQ=255)

                                                                                                                            tGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTAc                          >  1:1999178/1‑150 (MQ=255)

                                                                                                                               gATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGc                       <  1:428285/150‑1 (MQ=255)

                                                                                                                                    tCAGTATAAAGCCCACTTCTGACTTCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGGAGTAACGGATTACCACATACGTGGGTAATTTGCAGTACCGCACCCa                  >  2:357376/1‑150 (MQ=255)

                                                                                                                                      aGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGc                <  1:1687490/150‑1 (MQ=255)

                                                                                                                                        tataAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGcat              <  1:2874417/150‑1 (MQ=255)

                                                                                                                                        nataAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCACAAACGTTGGTAATTTGCAGTACCGCACCCAGcat              >  1:1165745/2‑150 (MQ=255)

                                                                                                                                         ataAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGcatc             >  1:538579/1‑150 (MQ=255)

                                                                                                                                         ataAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGcatc             <  2:173115/150‑1 (MQ=255)

                                                                                                                                          taAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGcatca            <  1:1198279/150‑1 (MQ=255)

                                                                                                                                           aaaGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGcatcat           <  2:74678/150‑1 (MQ=255)

                                                                                                                                             aGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATgg         <  2:3209353/150‑1 (MQ=255)

                                                                                                                                             aGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATgg         <  2:1933530/150‑1 (MQ=255)

                                                                                                                                                   cTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAaga   <  1:366408/150‑1 (MQ=255)

ACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAG  >  CP009273/2516405‑2516702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`