Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,619,125 G→T 19.1% G218C (GGT→TGT)  ppx → exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,619,1250GT19.1% 737.4 / 124.7 264G218C (GGT→TGT) ppxexopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base G (107/105);  new base T (26/25);  total (134/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

GTTGTGTCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCGG  >  CP009273/2618976‑2619274
                                                                                                                                                     |                                                                                                                                                     
gTTGTGTCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCg                                                                                                                                                       <  1:2020012/150‑1 (MQ=255)
gTTGTGTCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCg                                                                                                                                                       <  1:4210158/150‑1 (MQ=255)
gTTGTGTCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCg                                                                                                                                                       <  1:1457285/150‑1 (MQ=255)
   gtgtCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTa                                                                                                                                                    <  2:595956/150‑1 (MQ=255)
      tCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCa                                                                                                                                                 >  1:2518065/1‑150 (MQ=255)
       cAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGATGGAACGTTGCAATGGGCGCTTCCGGTACCAt                                                                                                                                                >  1:1629828/1‑150 (MQ=255)
             tGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAgc                                                                                                                                          >  2:483788/1‑150 (MQ=255)
               cccAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAgccg                                                                                                                                        >  2:309444/1‑150 (MQ=255)
                 cAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAgccgcc                                                                                                                                      >  1:3816465/1‑150 (MQ=255)
                    cTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCGCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCAt                                                                                                                                   <  2:2027481/150‑1 (MQ=255)
                     tttATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATg                                                                                                                                  >  1:522629/1‑150 (MQ=255)
                     tttATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATg                                                                                                                                  >  2:2110401/1‑150 (MQ=255)
                     tttATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCCGTACCATAAAAGCCGCCCATg                                                                                                                                  >  1:3854613/1‑150 (MQ=255)
                       tATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGaa                                                                                                                                >  2:462535/1‑150 (MQ=255)
                            tCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGtt                                                                                                                           <  1:2001887/150‑1 (MQ=255)
                                      gTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATg                                                                                                                 <  2:638680/150‑1 (MQ=255)
                                       tcatcaATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATgg                                                                                                                <  1:4345887/150‑1 (MQ=255)
                                        catcaATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGACCGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGACGTGTTAATGGAAATggg                                                                                                               >  2:3017906/1‑150 (MQ=255)
                                        catcaATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATggg                                                                                                               >  2:2337015/1‑150 (MQ=255)
                                         atcaATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGc                                                                                                              >  2:828323/1‑150 (MQ=255)
                                          tcaATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCg                                                                                                             <  1:4224970/150‑1 (MQ=255)
                                           caATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCga                                                                                                            <  2:104665/150‑1 (MQ=255)
                                            aaTAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCgag                                                                                                           <  1:396538/150‑1 (MQ=255)
                                            aaTAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCgag                                                                                                           >  2:861075/1‑150 (MQ=255)
                                             aTAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCgaga                                                                                                          >  1:631040/1‑150 (MQ=255)
                                             aTAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCgaga                                                                                                          >  1:2534646/1‑150 (MQ=255)
                                              tAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGaa                                                                                                         >  2:3436507/1‑150 (MQ=255)
                                               aaaGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGaaa                                                                                                        >  1:3248076/1‑150 (MQ=255)
                                                aaGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAg                                                                                                       <  1:477498/150‑1 (MQ=255)
                                                  gagaATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGAc                                                                                                     <  1:2189948/150‑1 (MQ=255)
                                                    gaATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCAGGAAGTGTTAATGGAAATGGGCGAGAAAGACgg                                                                                                   >  2:1081431/1‑150 (MQ=255)
                                                         ttCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATaa                                                                                              <  1:51449/150‑1 (MQ=255)
                                                           cAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAAtt                                                                                            <  2:3955530/150‑1 (MQ=255)
                                                            aGCGAGCTCGCATGGCGGCAGCACTAAAACTGGAATCTTTAACGTGTCAATTCCGTATTCAGGGCTGTATCGTTGCAAGGGGCGCTTCCGGTACCATAAAAGCCGGCCATGAAGTTTTTATGGAAATGGGGGAGTAAGACGGGATAATTa                                                                                           <  2:2595688/150‑1 (MQ=255)
                                                             gcgcgcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTAc                                                                                          <  1:3997824/150‑1 (MQ=255)
                                                                 gcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCg                                                                                      <  1:540648/150‑1 (MQ=255)
                                                                 gcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCg                                                                                      >  2:1192405/1‑150 (MQ=255)
                                                                       aTGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCATGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGt                                                                                >  1:3559713/1‑150 (MQ=255)
                                                                         ggcggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCt                                                                              <  1:1427492/150‑1 (MQ=255)
                                                                         ggcggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCt                                                                              <  1:2987364/150‑1 (MQ=255)
                                                                          gcggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTg                                                                             >  1:2042137/1‑150 (MQ=255)
                                                                          gcggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTg                                                                             <  1:3692985/150‑1 (MQ=255)
                                                                           cggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTgg                                                                            >  1:4050095/1‑150 (MQ=255)
                                                                             gcagcaCAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGaa                                                                          <  1:116308/150‑1 (MQ=255)
                                                                              cagcaCAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGaaa                                                                         >  1:3525168/1‑150 (MQ=255)
                                                                              cagcaCAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGaaa                                                                         >  1:3524710/1‑150 (MQ=255)
                                                                               agcaCAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGaaaa                                                                        >  2:2240423/1‑150 (MQ=255)
                                                                               agcaCAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGaaaa                                                                        <  2:213111/150‑1 (MQ=255)
                                                                                gcaCAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGaaaaa                                                                       <  1:3569227/150‑1 (MQ=255)
                                                                                    aaaaaCTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTgg                                                                   <  1:3480657/150‑1 (MQ=255)
                                                                                     aaaaCTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGt                                                                  <  2:1959001/150‑1 (MQ=255)
                                                                                       aaCTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTaa                                                                <  2:4201676/150‑1 (MQ=255)
                                                                                       aaCTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTaa                                                                <  1:1217357/150‑1 (MQ=255)
                                                                                       aaCTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTaa                                                                <  2:960143/150‑1 (MQ=255)
                                                                                       aaCTGGAAACTATAACCTGGCAAGTCCGTATTCAGGGCTGGAACGGTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAAGGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTaa                                                                <  2:4032973/150‑1 (MQ=255)
                                                                                       aaCTGGAAACTAGAACCTGGCAATTCCGTATTCAGGGCTGGAACGGTGCAAGGGGCGCGTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTac                                                                <  1:551484/150‑2 (MQ=255)
                                                                                          tGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAaag                                                             >  2:926663/1‑150 (MQ=255)
                                                                                           ggAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAaaga                                                            >  2:4097815/1‑150 (MQ=255)
                                                                                           ggAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAaaga                                                            >  1:1306840/1‑150 (MQ=255)
                                                                                            gAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAaagaa                                                           >  2:1437843/1‑150 (MQ=255)
                                                                                              aaCTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGACGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGACCGTCTGGAAAAACTGGTAAAAGAAGt                                                         >  2:2854485/1‑150 (MQ=255)
                                                                                              aaCTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGt                                                         >  2:1813943/1‑150 (MQ=255)
                                                                                              aaCTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGt                                                         <  2:358972/150‑1 (MQ=255)
                                                                                                cTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGttt                                                       >  2:3361115/1‑150 (MQ=255)
                                                                                                 tttAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGtttt                                                      <  1:3435084/150‑1 (MQ=255)
                                                                                                 tttAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGtttt                                                      >  2:2973795/1‑150 (MQ=255)
                                                                                                   tAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTAc                                                    >  1:1807071/1‑150 (MQ=255)
                                                                                                    aaCCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACg                                                   >  1:1620769/1‑150 (MQ=255)
                                                                                                       cTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCa                                                <  1:883067/150‑1 (MQ=255)
                                                                                                       cTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCa                                                <  1:3888378/150‑1 (MQ=255)
                                                                                                       cTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCa                                                <  2:668620/150‑1 (MQ=255)
                                                                                                            aaTTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTa                                           <  1:2122296/150‑1 (MQ=255)
                                                                                                                ccGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAAttt                                       <  2:2496828/150‑1 (MQ=255)
                                                                                                                 cGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTc                                      <  1:2243950/150‑1 (MQ=255)
                                                                                                                      tCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATc                                 <  1:2774171/150‑1 (MQ=255)
                                                                                                                      tCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATc                                 >  1:3798304/1‑150 (MQ=255)
                                                                                                                       cAGGGCTGGACCGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGACGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCg                                >  2:1311922/1‑150 (MQ=255)
                                                                                                                       cAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCg                                <  1:1001826/150‑1 (MQ=255)
                                                                                                                         gggCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCt                              >  2:2487997/1‑150 (MQ=255)
                                                                                                                         gggCTGGAACGTTGCAATGGGCGCCTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGACCGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCt                              >  2:1660849/1‑150 (MQ=255)
                                                                                                                          ggCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTg                             >  2:1858774/1‑150 (MQ=255)
                                                                                                                             tGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGt                          >  1:4107155/1‑150 (MQ=255)
                                                                                                                             tGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGt                          >  1:4285597/1‑150 (MQ=255)
                                                                                                                               gAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGttt                        <  2:522629/150‑1 (MQ=255)
                                                                                                                               gAACGGTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGttt                        <  2:3486090/150‑1 (MQ=255)
                                                                                                                                aaCGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAAAGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTa                       >  1:956951/1‑150 (MQ=255)
                                                                                                                                   gTTGCAATGGGCGCTTCCGGTACCATAAAAGCCTCCCATGAAGTGTTAATGTAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCg                    <  1:3322756/150‑1 (MQ=255)
                                                                                                                                   gTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCg                    >  2:3228786/1‑150 (MQ=255)
                                                                                                                                   gTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCg                    >  1:3171724/1‑150 (MQ=255)
                                                                                                                                   gTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCg                    <  1:767462/150‑1 (MQ=255)
                                                                                                                                   gTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCg                    >  2:1568388/1‑150 (MQ=255)
                                                                                                                                   gTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTAAGTCACCGTAATTTCGCATCGCTGAGTTTAACg                    >  1:30708/1‑150 (MQ=255)
                                                                                                                                      gCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGt                 <  2:3544230/150‑1 (MQ=255)
                                                                                                                                      gCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGt                 >  1:3373039/1‑150 (MQ=255)
                                                                                                                                        aaTGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCt               >  2:39020/1‑150 (MQ=255)
                                                                                                                                         aTGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCtt              <  1:1051386/150‑1 (MQ=255)
                                                                                                                                          tGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCttt             <  1:2678123/150‑1 (MQ=255)
                                                                                                                                            ggCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTcc           >  1:2438341/1‑150 (MQ=255)
                                                                                                                                                cTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAag       >  2:1788610/1‑150 (MQ=255)
                                                                                                                                                     ggTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCgg  >  2:3703323/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTGTGTCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCGG  >  CP009273/2618976‑2619274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑