Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,619,563 C→A 8.4% R364R (CGA→AGA)  ppx → exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,619,5630CA 8.4% 1200.6 / 55.5 323R364R (CGA→AGA) ppxexopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base C (142/153);  new base A (14/14);  total (156/167)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 323 total aligned reads displayed.

CGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACC  >  CP009273/2619416‑2619710
                                                                                                                                                   |                                                                                                                                                   
cGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGa                                                                                                                                                   <  1:3967474/150‑1 (MQ=255)
  tAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATg                                                                                                                                                 >  1:4000709/1‑150 (MQ=255)
   aGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATgg                                                                                                                                                >  1:3689125/1‑150 (MQ=255)
   aGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATgg                                                                                                                                                <  1:909833/150‑1 (MQ=255)
    gTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATggg                                                                                                                                               >  2:4053753/1‑150 (MQ=255)
           cGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCAt                                                                                                                                        >  2:1730845/1‑150 (MQ=255)
           cGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCAt                                                                                                                                        <  1:2111670/150‑1 (MQ=255)
              cagcagCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCAtgct                                                                                                                                     <  2:3242324/150‑1 (MQ=255)
               agcagcCTCGCCAACCAGTATCACATCGACAGCGACCAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCACCTGGAGGCGCTACTGCGATGGGCCGCCAtgctg                                                                                                                                    >  2:3836118/1‑150 (MQ=255)
                 cagcCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCa                                                                                                                                  <  1:4217665/150‑1 (MQ=255)
                  agcCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCAt                                                                                                                                 >  2:3661342/1‑150 (MQ=255)
                   gcCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATg                                                                                                                                <  1:520309/150‑1 (MQ=255)
                    ccTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGa                                                                                                                               <  2:1620784/150‑1 (MQ=255)
                     cTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAg                                                                                                                              <  1:2372785/150‑1 (MQ=255)
                     cTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAg                                                                                                                              <  2:337566/150‑1 (MQ=255)
                        gCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTc                                                                                                                           <  2:2554210/150‑1 (MQ=255)
                          cAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCgg                                                                                                                         >  1:4282429/1‑150 (MQ=255)
                           aaCCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCggg                                                                                                                        <  2:3794685/150‑1 (MQ=255)
                            aCCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGt                                                                                                                       <  1:860320/150‑1 (MQ=255)
                             ccAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGtt                                                                                                                      <  2:3324080/150‑1 (MQ=255)
                              cAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTg                                                                                                                     >  1:2600687/1‑150 (MQ=255)
                              cAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTg                                                                                                                     >  1:77597/1‑150 (MQ=255)
                              cAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTg                                                                                                                     >  1:3284033/1‑150 (MQ=255)
                                 tATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAat                                                                                                                  <  1:589415/150‑1 (MQ=255)
                                 tATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAat                                                                                                                  <  1:3428789/150‑1 (MQ=255)
                                   tCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAatat                                                                                                                >  1:450408/1‑150 (MQ=255)
                                    cacaTCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATc                                                                                                               <  1:2083728/150‑1 (MQ=255)
                                     acaTCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCa                                                                                                              >  1:1277048/1‑150 (MQ=255)
                                       aTCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAAc                                                                                                            <  2:1471045/150‑1 (MQ=255)
                                         cGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACca                                                                                                          >  2:4215573/1‑150 (MQ=255)
                                         cGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACca                                                                                                          >  2:2738134/1‑150 (MQ=255)
                                          gACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACcac                                                                                                         >  2:2740687/1‑150 (MQ=255)
                                           aCAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACcaca                                                                                                        >  1:2984487/1‑150 (MQ=255)
                                            cAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAg                                                                                                       >  1:2054404/1‑150 (MQ=255)
                                              gCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCg                                                                                                     <  1:3100830/150‑1 (MQ=255)
                                                gAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGt                                                                                                   >  1:2936696/1‑150 (MQ=255)
                                                gAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGt                                                                                                   <  1:3950033/150‑1 (MQ=255)
                                                  aCAGGCCCGTCGGGTGCTGGATTCCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCTTCCGCATCTGGAGGCGCTACTGCGATGGGCCTCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGttt                                                                                                 <  2:1796508/150‑1 (MQ=255)
                                                  aCAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGttt                                                                                                 >  1:240255/1‑150 (MQ=255)
                                                   cAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTg                                                                                                >  1:623203/1‑150 (MQ=255)
                                                    aGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGc                                                                                               <  1:3473858/150‑1 (MQ=255)
                                                       cccGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATc                                                                                            >  1:1594191/1‑150 (MQ=255)
                                                        ccGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCg                                                                                           <  1:2239853/150‑1 (MQ=255)
                                                          gACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGcc                                                                                         >  1:135474/1‑150 (MQ=255)
                                                            cGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCAc                                                                                       <  2:4000709/150‑1 (MQ=255)
                                                               gTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTcc                                                                                    <  1:1150084/150‑1 (MQ=255)
                                                                tGCTGGATACCACTATGCAAATGTACGACCAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCg                                                                                   <  1:1974537/150‑1 (MQ=255)
                                                                tGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCg                                                                                   >  2:324630/1‑150 (MQ=255)
                                                                tGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCg                                                                                   >  2:1595121/1‑150 (MQ=255)
                                                                tGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCg                                                                                   >  2:2443428/1‑150 (MQ=255)
                                                                tGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCg                                                                                   <  1:4124568/150‑1 (MQ=255)
                                                                   tGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCtt                                                                                <  2:396801/150‑1 (MQ=255)
                                                                    ggATACCACTATGCAAATGTACGAAGAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTta                                                                               <  2:795651/150‑1 (MQ=255)
                                                                     gATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTtat                                                                              >  1:3321772/1‑150 (MQ=255)
                                                                      aTACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTtata                                                                             <  1:1758667/150‑1 (MQ=255)
                                                                      aTACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTtata                                                                             >  2:3755469/1‑150 (MQ=255)
                                                                      aTACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTtata                                                                             >  2:2765181/1‑150 (MQ=255)
                                                                       tACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTatat                                                                            >  1:1772882/1‑150 (MQ=255)
                                                                            cTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGc                                                                       <  1:987515/150‑1 (MQ=255)
                                                                            cTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGc                                                                       >  1:1825057/1‑150 (MQ=255)
                                                                            cTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGc                                                                       >  1:3776566/1‑150 (MQ=255)
                                                                                   aaTGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAg                                                                >  2:288778/1‑150 (MQ=255)
                                                                                    aTGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGt                                                               <  1:1013378/150‑1 (MQ=255)
                                                                                    aTGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGt                                                               >  2:209832/1‑150 (MQ=255)
                                                                                    aTGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGt                                                               >  2:211343/1‑150 (MQ=255)
                                                                                     tGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTg                                                              <  2:2416852/150‑1 (MQ=255)
                                                                                         cGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACtt                                                          <  2:3394211/150‑1 (MQ=255)
                                                                                         cGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACtt                                                          >  1:1476283/1‑150 (MQ=255)
                                                                                         cGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACtt                                                          <  2:4168653/150‑1 (MQ=255)
                                                                                         cGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACtt                                                          <  1:3182077/150‑1 (MQ=255)
                                                                                          gAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTg                                                         >  1:3565043/1‑150 (MQ=255)
                                                                                               gTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCggg                                                    <  1:3125561/150‑1 (MQ=255)
                                                                                               gTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCggg                                                    <  1:1990965/150‑1 (MQ=255)
                                                                                                 ggCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGtt                                                  <  2:3565406/150‑1 (MQ=255)
                                                                                                  gCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGttt                                                 <  2:3580140/150‑1 (MQ=255)
                                                                                                   cGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGtttt                                                >  2:4310735/1‑150 (MQ=255)
                                                                                                    gggAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTa                                               >  1:25330/1‑150 (MQ=255)
                                                                                                      gAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAAt                                             >  2:249022/1‑150 (MQ=255)
                                                                                                           gCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGa                                        >  2:11404/1‑150 (MQ=255)
                                                                                                            cAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGaa                                       >  1:3383257/1‑150 (MQ=255)
                                                                                                            cAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGaa                                       <  2:379939/150‑1 (MQ=255)
                                                                                                               ccGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAAcag                                    <  1:2174589/150‑1 (MQ=255)
                                                                                                                  aaGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAAcagcag                                 >  1:2666115/1‑150 (MQ=255)
                                                                                                                  aaGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAAcagcag                                 >  1:3071173/1‑150 (MQ=255)
                                                                                                                    gCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCt                               <  2:736779/150‑1 (MQ=255)
                                                                                                                     cTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCtg                              <  1:4199424/150‑1 (MQ=255)
                                                                                                                       ggCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCtgat                            >  1:4098589/1‑150 (MQ=255)
                                                                                                                       ggCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCtgat                            <  1:1029533/150‑1 (MQ=255)
                                                                                                                             tCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGc                      <  2:1855020/150‑1 (MQ=255)
                                                                                                                                   aCTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACt                >  2:1382723/1‑150 (MQ=255)
                                                                                                                                   aCTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACt                <  2:2172413/150‑1 (MQ=255)
                                                                                                                                     tGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTgg              <  1:2923465/150‑1 (MQ=255)
                                                                                                                                         ggCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTgcg          <  2:2905575/150‑1 (MQ=255)
                                                                                                                                           cgcTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCt        <  1:2765181/150‑1 (MQ=255)
                                                                                                                                           cgcTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCt        <  1:3755469/150‑1 (MQ=255)
                                                                                                                                            gcTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTa       >  2:2952935/1‑150 (MQ=255)
                                                                                                                                               aCTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCa    <  2:148347/150‑1 (MQ=255)
                                                                                                                                               aCTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCa    <  2:1921513/150‑1 (MQ=255)
                                                                                                                                                 tGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCAcc  >  1:1561055/1‑150 (MQ=255)
                                                                                                                                                 tGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTGTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCAcc  >  2:2266162/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACC  >  CP009273/2619416‑2619710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑