Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,711,673 A→C 9.8% V72G (GTT→GGT)  yfiF ← putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,711,6730AC 9.8% 623.0 / 5.0 245V72G (GTT→GGT) yfiFputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (167/45);  new base C (2/31);  total (169/76)
Fisher's exact test for biased strand distribution p-value = 3.30e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

TCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGA  >  CP009273/2711524‑2711821
                                                                                                                                                     |                                                                                                                                                    
   ggAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg                                                                                                                                                   <  2:778596/150‑1 (MQ=255)
    gAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt                                                                                                                                                  >  1:1074544/1‑150 (MQ=255)
    gAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt                                                                                                                                                  <  2:221460/150‑1 (MQ=255)
    gAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt                                                                                                                                                  <  2:2109898/150‑1 (MQ=255)
    gAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt                                                                                                                                                  >  1:275613/1‑150 (MQ=255)
      aaaGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTgc                                                                                                                                                >  2:2404168/1‑150 (MQ=255)
       aaGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTgcg                                                                                                                                               >  1:1434926/1‑150 (MQ=255)
              tGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGc                                                                                                                                        >  1:3822797/1‑150 (MQ=255)
                aCATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGa                                                                                                                                      >  1:2325026/1‑150 (MQ=255)
                    gCATTTTCGCCGTTGACGGGGGTTTCTCCCGCACGCTGACGACGAAACACTACCGGAAAAAAAAAACTTTTACCCCTGATCCCACCGTGATCGGCCTTTTCCGGCGTATCATCACCCGGCGCGCGGTAAACGGGGCGCCACGGCGAATCt                                                                                                                                  <  2:3626686/150‑1 (MQ=255)
                      aTTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTc                                                                                                                                >  2:2164886/1‑150 (MQ=255)
                      aTTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTc                                                                                                                                >  2:1407130/1‑150 (MQ=255)
                       ttttCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTcc                                                                                                                               >  2:1808433/1‑150 (MQ=255)
                        tttCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGACACCGTGCGCCACGGCGAATCTTccc                                                                                                                              >  2:823763/1‑150 (MQ=255)
                        tttCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTccc                                                                                                                              >  2:823890/1‑150 (MQ=255)
                         ttCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCa                                                                                                                             >  1:487549/1‑150 (MQ=255)
                          gcGCCGTCGCCGGGGGGTTCTGCCCCACGCGGACGCCCCAACACTGCCGGAAAAATAAAAGTTTACCCCCGGCGCCCCCCGTGAGCGGCTTTTCCGGGCGTCTCACCCCCCGGGGCGGGGGAACCGGGGCGCCACGGCGAATCTTCCCAc                                                                                                                            <  2:176594/149‑1 (MQ=255)
                           cGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACt                                                                                                                           >  2:2299497/1‑150 (MQ=255)
                              cGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGGGTCTCATCACCCGGCGCGCGGGACACCGTGCGCCACGGCGAATCTTCCCACTTGc                                                                                                                        >  1:2203248/1‑150 (MQ=255)
                                tAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCgg                                                                                                                      >  2:3284004/1‑150 (MQ=255)
                                           ttcttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCTTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTg                                                                                                           >  1:285648/1‑150 (MQ=255)
                                           ttcttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTg                                                                                                           >  2:237216/1‑150 (MQ=255)
                                             cttcCGCACGCTGACGCCGCAACACTTCCGGATAAATAAAACTTTTACCACTGTTGCCACCGTGATCGGCCTTTGCGGGCGTCTCATCCCCCGGCGCGGGGTAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc                                                                                                         <  2:2477755/150‑1 (MQ=255)
                                             cttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc                                                                                                         >  1:2496565/1‑150 (MQ=255)
                                              ttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAct                                                                                                        >  1:792558/1‑150 (MQ=255)
                                                 cgccggCTGCCGACCAAACACTCCGGGATAAATAAAACTTTCCCCACTGAGCCCCCGGGGCGGGGCGTTGGCCGGGGTCTCAACCCCGGGGGCGGGGTAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTg                                                                                                     <  2:3647309/145‑1 (MQ=255)
                                                  gCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt                                                                                                    >  1:141006/1‑150 (MQ=255)
                                                   cACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt                                                                                                   >  2:380947/1‑150 (MQ=255)
                                                     cGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg                                                                                                 >  2:143018/1‑150 (MQ=255)
                                                     cGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg                                                                                                 >  2:143459/1‑150 (MQ=255)
                                                        tgccgacgaAACCCGTCCGGAAAAATAAAAATTTTACCACTGAGCCCACCGTGATCGGCGTTTTCGGGCTTCTCAACCCCCGGGGGGCGGCAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCa                                                                                              <  2:1692163/147‑1 (MQ=255)
                                                          actacgAAACACTCCCGCATAAAAAAAAATTTAACCCCTGACGCCCCCTTGATCGGCATTGCCCGGCGACTCAACCCCGGGCGGGGGGCAAACCGCCCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATc                                                                                            <  1:2779020/147‑1 (MQ=255)
                                                           cgaccCAAAACCTCCTCATAAATAAAAATTTAACCACAGTTGCCAGGTTGATCGCCCTTTTCCGGCGTATAATCACCCGGCGCGCGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATcg                                                                                           <  2:2695197/150‑1 (MQ=255)
                                                            gacgCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATcgc                                                                                          >  1:3197212/1‑150 (MQ=255)
                                                                cAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggc                                                                                      >  1:4196299/1‑150 (MQ=255)
                                                                 aaCACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcg                                                                                     >  2:1933854/1‑150 (MQ=255)
                                                                 aaCACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcg                                                                                     >  1:91086/1‑150 (MQ=255)
                                                                 aaCACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcg                                                                                     >  2:145074/1‑150 (MQ=255)
                                                                 aaCACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcg                                                                                     >  1:452124/1‑150 (MQ=255)
                                                                   cacTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg                                                                                   >  2:350070/1‑150 (MQ=255)
                                                                     ctcccGCATCAATAAAAATTTAACCACTGAGGCCACCGTGCGCGGCCTTTACCGGCGTCTCATCACCCGGCGCGCGGTAAACCGTCCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcggggc                                                                                 <  2:3860760/147‑1 (MQ=255)
                                                                     cTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcggggc                                                                                 >  1:763162/1‑150 (MQ=255)
                                                                       tCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGa                                                                               >  2:3498534/1‑150 (MQ=255)
                                                                        ccGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGAc                                                                              >  1:1021623/1‑150 (MQ=255)
                                                                             tCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCa                                                                         >  2:1405178/1‑150 (MQ=255)
                                                                              cAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAc                                                                        >  2:1306700/1‑150 (MQ=255)
                                                                              cAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAc                                                                        >  2:2558593/1‑150 (MQ=255)
                                                                              cAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAc                                                                        >  1:1397188/1‑150 (MQ=255)
                                                                                aTAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCg                                                                      >  2:3230149/1‑150 (MQ=255)
                                                                                 tAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGt                                                                     >  1:2340375/1‑150 (MQ=255)
                                                                                  aaaaCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTc                                                                    >  1:3882264/1‑150 (MQ=255)
                                                                                  aaaaCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTc                                                                    >  1:1902805/1‑150 (MQ=255)
                                                                                   aaaCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCa                                                                   >  1:3313736/1‑150 (MQ=255)
                                                                                    aaCTTTAACCCCTGCTCCCACGTTGATCGGCCTTTGCGGGCGTCCAATCACCGGGCGCGGGGCAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAg                                                                  <  2:3516877/150‑1 (MQ=255)
                                                                                     aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGc                                                                 >  2:3554014/1‑150 (MQ=255)
                                                                                            ccACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAt                                                          >  2:4304545/1‑150 (MQ=255)
                                                                                             cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAtt                                                         >  1:3678515/1‑150 (MQ=255)
                                                                                             cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAtt                                                         >  2:2768676/1‑150 (MQ=255)
                                                                                                tGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTc                                                      >  1:3420928/1‑150 (MQ=255)
                                                                                                 gaggCCACGGTGAGCGGCCTTTGCGGGCGTCTCATCCCCCGGCGCGGGGTAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTcc                                                     <  2:4186127/147‑1 (MQ=255)
                                                                                                 gATGCCACGGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGGGCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTcc                                                     <  2:3219594/150‑1 (MQ=255)
                                                                                                  aTGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCt                                                    >  1:1457825/1‑150 (MQ=255)
                                                                                                   gcccACGGTGATCGGCCTTTTCCGGCGTCACATCCCCCGGCGGGCGGGAACCCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTg                                                   <  1:2899635/148‑1 (MQ=255)
                                                                                                     ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGa                                                 >  1:1589182/1‑150 (MQ=255)
                                                                                                      cACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAc                                                >  1:1802453/1‑150 (MQ=255)
                                                                                                      cACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAc                                                >  2:3383264/1‑150 (MQ=255)
                                                                                                      cACCGTGAGCGGCCTTGTCCGGCGTCTCATCACCCGGCGCGCGGCAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAc                                                <  2:4322436/150‑1 (MQ=255)
                                                                                                       aCCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcg                                               >  2:3930691/1‑150 (MQ=255)
                                                                                                       aCCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcg                                               >  2:2750623/1‑150 (MQ=255)
                                                                                                       aCCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcg                                               >  2:412292/1‑150 (MQ=255)
                                                                                                       aCCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcg                                               >  2:412531/1‑150 (MQ=255)
                                                                                                        ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgc                                              >  2:1112290/1‑150 (MQ=255)
                                                                                                        ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgc                                              >  2:3098566/1‑150 (MQ=255)
                                                                                                        ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAGTTTCCTGGAcgc                                              >  2:3485067/1‑150 (MQ=255)
                                                                                                          gTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgccc                                            >  1:170576/1‑150 (MQ=255)
                                                                                                           tGAGCGGCCTGTGCGGGCGTCTCATCCCCGGGCGCGGGGTAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccg                                           <  2:4062093/150‑1 (MQ=255)
                                                                                                            gATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccgc                                          >  1:210517/1‑150 (MQ=255)
                                                                                                            gATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccgc                                          >  1:211272/1‑150 (MQ=255)
                                                                                                            gATCGGCCTTTTCCGGCGTCTAATCACCAGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccgc                                          >  2:2816772/1‑150 (MQ=255)
                                                                                                              tgggCATTTCCCGGCGTCTCATCACCGGGCGCGGGGTAAACGGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACgcccgccc                                        <  1:2518904/148‑1 (MQ=255)
                                                                                                               cGGCCTTTTCCGGCGTCTCAACACCCGGCGCGCGGGAACCCGTCCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGcccc                                       <  2:1636130/150‑1 (MQ=255)
                                                                                                                ggCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGccccc                                      >  2:2951924/1‑150 (MQ=255)
                                                                                                                   cTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCttt                                   >  2:2569133/1‑150 (MQ=255)
                                                                                                                     tttCGGGGGTCTCATCACCGGGCGCGGGGCAACCGGGGCGCCACGGCGAATCTTCCCACGTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTcc                                 <  2:2596890/150‑1 (MQ=255)
                                                                                                                      tgcgggCGTCCCATCCCCCGGCGCGGGGGAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTccc                                <  2:1590465/146‑1 (MQ=255)
                                                                                                                      tcccGGGGTCTCAGCACCCGGCGGGCGTGAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTccc                                <  2:3779249/148‑1 (MQ=255)
                                                                                                                       gcgggCGTATCACCACCCGGCGCGGGGGAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCg                               <  1:4333337/147‑1 (MQ=255)
                                                                                                                         gggCGTCTCAGCACCGGGCGGGGGGTAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGt                             <  2:2650681/149‑1 (MQ=255)
                                                                                                                         cGGCGTCACATCCCGCGGGGGGGGGGACACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGt                             <  2:598210/150‑1 (MQ=255)
                                                                                                                          ggCTTCTCAACCCCCGGCGCGCGGCAACCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTa                            <  2:4072001/150‑1 (MQ=255)
                                                                                                                             gTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcgc                         >  2:2892573/1‑150 (MQ=255)
                                                                                                                                 caccccccGGCGCGCGGCAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGt                     <  1:1897757/145‑1 (MQ=255)
                                                                                                                                   accccgggCGCGCGGTAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGTACGCCCGCCCCCTTTCCCGGTACGCGGGTtg                   <  1:2138162/144‑1 (MQ=255)
                                                                                                                                    caCCGGGCGCGGGGTAACGGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtgt                  <  2:3057599/150‑1 (MQ=255)
                                                                                                                                    caCCCGGCGCGCGGGAACCCGTGCGCCACGGCGAATCTAACCACTTGCGGGCACGGGGCTGACTCTGTATGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCATGGACGCCCGCCCCCATTCCCGGTACGCTGGTtgt                  >  2:1028620/1‑150 (MQ=255)
                                                                                                                                    caCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtgt                  >  2:758262/1‑150 (MQ=255)
                                                                                                                                         ggCGCGCGCGACCCGGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTa             <  1:3519648/150‑1 (MQ=255)
                                                                                                                                               cgGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTa       >  1:1525443/1‑150 (MQ=255)
                                                                                                                                                 ggAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATc     >  2:1969313/1‑150 (MQ=255)
                                                                                                                                                 ggAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATc     >  1:1295046/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGA  >  CP009273/2711524‑2711821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑