Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,908,686 G→T 10.7% E91* (GAA→TAA)  barA → hybrid sensory histidine kinase, in two‑component regulatory system with UvrY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,908,6860GT10.7% 578.2 / 3.1 220E91* (GAA→TAA) barAhybrid sensory histidine kinase, in two‑component regulatory system with UvrY
Reads supporting (aligned to +/- strand):  ref base G (122/51);  new base T (4/34);  total (126/94)
Fisher's exact test for biased strand distribution p-value = 5.59e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 238 total aligned reads displayed.

TGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGCACGCCGATTATTTCTGAGAGTTA  >  CP009273/2908537‑2908834
                                                                                                                                                     |                                                                                                                                                    
tGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCCTTCCGATATTGTTCGCGCGATTTCGGTTTatgatg                                                                                                                                                      >  1:4297313/1‑150 (MQ=255)
 ggAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTAtgatga                                                                                                                                                     <  2:2817924/150‑1 (MQ=255)
  gAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGaa                                                                                                                                                    <  1:651103/150‑1 (MQ=255)
  gAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGaa                                                                                                                                                    <  2:3304118/150‑1 (MQ=255)
   aaGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGaaa                                                                                                                                                   <  2:879684/150‑1 (MQ=255)
   aaGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGaaa                                                                                                                                                   <  2:3057764/150‑1 (MQ=255)
     gATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAAt                                                                                                                                                 <  2:681727/150‑1 (MQ=255)
      aTGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATa                                                                                                                                                <  2:2642999/150‑1 (MQ=255)
               ccAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAATCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATTAAAATAACCGActct                                                                                                                                       >  2:3699421/1‑150 (MQ=255)
               ccAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGActct                                                                                                                                       >  2:3626210/1‑150 (MQ=255)
               ccAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTAATGCATAGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGAatct                                                                                                                                       >  2:2745448/1‑150 (MQ=255)
                cAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCtt                                                                                                                                      >  2:911897/1‑150 (MQ=255)
                  gCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGAGCCAGCAAAATCGCGAATATATCGGTCAGTTAAAAAGCGTACTGCATCGTCGCCATTCCGAAATTGTTCGCGCGATTTCGGTTTATGATTAAAATAAACGACTCTTTg                                                                                                                                    >  1:2219645/1‑150 (MQ=255)
                    attattGAGCCGCTTGCAGTTTCTACTGAATATGGCATGATCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATTATGAAAATAACCGACTCTTTGTc                                                                                                                                  >  1:3217004/1‑150 (MQ=255)
                    attattGAGCCGCTTGCAGTTTCTACTGAAAATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTc                                                                                                                                  >  1:701759/1‑150 (MQ=255)
                     ttattGAGCCGCTTGCAGTTTCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCa                                                                                                                                 >  2:3761365/1‑150 (MQ=255)
                       attGAGCCGCTTGCAGTTTCTACTGAATATTGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATAGTCGCCATTACGATATTTTTAGCGCGATTTCGGTTTATGATGAAAATAAACGACACTTTGTCAcc                                                                                                                               >  1:2336986/1‑150 (MQ=255)
                       attGAGCCGCTTGCAGTTTCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCAcc                                                                                                                               >  2:1364998/1‑150 (MQ=255)
                                tttGCATTTTCTACTTATTATGGCATGAGCGTGAAAAATCGCTAATCTATCGTTCTTTTAATAAGCGTACTGATTCGTCGCCATTCCTATATTGTTCGCGGGTTTTGGGTTTTTTATTAAATTAACCGACTCTTTGTCACCTCCAAtttt                                                                                                                      <  2:1009543/149‑1 (MQ=255)
                                ctttatgTTTATAATCAATATGTCATTAGCCTTCAAAATCGCTAATATATCGGTATTTTAATACGCGTACTTCTTCGTCGCCTTTCCTATTTTGTTCGCGCCATTTCGTTTTTTTATTAAAATAACCGACTCTTTGTCACCTCCAAtttt                                                                                                                      <  2:3269296/144‑1 (MQ=255)
                                   gctgTTTCTACTGAATATGGCATGAGCCTGCAAATTCGCGAATCTATCGGACAGTTAATAAGCGTACTGCATCGTCGCCATGCCGATATTGTTCGCGCTATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCAt                                                                                                                   <  2:1788885/147‑1 (MQ=255)
                                    cAGTTTCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATc                                                                                                                  >  2:2999828/1‑150 (MQ=255)
                                       tttCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTg                                                                                                               >  2:1135816/1‑150 (MQ=255)
                                        ttCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGa                                                                                                              >  2:2157601/1‑150 (MQ=255)
                                        ttCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGa                                                                                                              >  2:577163/1‑150 (MQ=255)
                                          cTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATc                                                                                                            >  1:2772365/1‑150 (MQ=255)
                                            aCTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATccc                                                                                                          >  2:2287727/1‑150 (MQ=255)
                                              tttatttGGCATGAGCCTGAAAATTCGCTAATCTATGGGTCTTTTATAAAGCGTACTGCATGGTCGCAATTCCTATATTGTTCGCGCGTTTTCGGTTTTTGATTAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCtc                                                                                                        <  2:1945250/144‑1 (MQ=255)
                                                attatGGCATGAGCCTGCAAAATCGCTAATCTATGGGTCTTTAAATACGCGTCCTGCATCGACGCCATTCCGATTTTGTCCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCtcat                                                                                                      <  2:44170/148‑1 (MQ=255)
                                                aaTATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCtcat                                                                                                      >  2:15093/1‑150 (MQ=255)
                                                  tatGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCtcatca                                                                                                    >  1:2971763/1‑150 (MQ=255)
                                                    tGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAAt                                                                                                  >  1:1132663/1‑150 (MQ=255)
                                                    tGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCGTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAAt                                                                                                  >  2:4104584/1‑150 (MQ=255)
                                                    tGGAATGAGCCTGCAAAAGCGCGAATCTATCGGTCTGTTAATAAGCGTACTGCATCGTCGCCTTTCCTATATGGTTCGCGCTATTTCGTTTTATGATTAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAAt                                                                                                  <  2:1708454/150‑1 (MQ=255)
                                                     ggattGAGCCTGCAAAATCGCGACTCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCTTTATTGTTCGCGCGTTTTGGGTTTTTGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATg                                                                                                 <  2:3492232/146‑1 (MQ=255)
                                                     ggCATGAGCCTGCAAAATCGCTAATCTACCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTAATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATg                                                                                                 <  2:1425888/150‑1 (MQ=255)
                                                        aTGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAg                                                                                              >  1:3135644/1‑150 (MQ=255)
                                                        aTGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATATTGATCCCTCATCAATGCAg                                                                                              >  2:3077124/1‑150 (MQ=255)
                                                           aGCCTGAAAAATCGCTAATCTATCGGTCTTTTAATAAGCTTACTGCATCGTCGCCTTTCCTATTTTGTTCGCGCTTTTTCGTTTTATGATTAAAAAAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTc                                                                                           <  1:1569931/150‑1 (MQ=255)
                                                            gCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCg                                                                                          >  1:1832141/1‑150 (MQ=255)
                                                             ccTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCgg                                                                                         >  1:422944/1‑150 (MQ=255)
                                                                 cAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGgcagc                                                                                     >  1:2095906/1‑150 (MQ=255)
                                                                  aaaaTCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGgcagca                                                                                    >  1:452052/1‑150 (MQ=255)
                                                                       cgcgAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTg                                                                               >  2:3785904/1‑150 (MQ=255)
                                                                         cgAATCTATCGGTCATTTAATACGCGTACTGCATCGTCGCCATTCCTATTTTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGcc                                                                             <  1:315173/150‑1 (MQ=255)
                                                                              cTATCGTTCAGTTAATAAGCGTACTGCTTCGTCGCCATGCCTATATGGTTCGCGCGATTTCGTTTTATTATTAAAATACCCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTc                                                                        <  2:2400377/150‑1 (MQ=255)
                                                                                 tCGGTCATTTAATAAGCGTACTGCAGCGTCGCCATTCCGATTGTGTTCGCGCTTTTTCGGTTTATGATTAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTc                                                                     <  2:3813829/150‑1 (MQ=255)
                                                                                     tCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCa                                                                 >  1:1136341/1‑150 (MQ=255)
                                                                                        gTTAATACGCGTACTGCATCGTCGCCTTTCCGATATTGTTCGCGCGTTTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCt                                                              <  2:1267713/150‑1 (MQ=255)
                                                                                        gTTAATACGCGTACTGAATCGTCGCCATTCCGATTTTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCt                                                              <  2:698687/150‑1 (MQ=255)
                                                                                        gTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCt                                                              >  1:3591309/1‑150 (MQ=255)
                                                                                         ttAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTc                                                             >  1:3394796/1‑150 (MQ=255)
                                                                                          taataaGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCa                                                            >  2:1227640/1‑150 (MQ=255)
                                                                                           aataaGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCAc                                                           >  2:1698903/1‑150 (MQ=255)
                                                                                           aataaGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCAc                                                           >  2:169116/1‑150 (MQ=255)
                                                                                           aataaGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCAc                                                           >  2:3887717/1‑150 (MQ=255)
                                                                                            atacGCGTAGTGCATGGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACt                                                          <  2:1150565/150‑1 (MQ=255)
                                                                                            ataaGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACt                                                          >  2:3125085/1‑150 (MQ=255)
                                                                                             taaGCGTACTGAATCTTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTg                                                         <  2:3548906/150‑1 (MQ=255)
                                                                                               aGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTc                                                       >  1:1991737/1‑150 (MQ=255)
                                                                                               aGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTc                                                       >  1:3909149/1‑150 (MQ=255)
                                                                                                 cGTAGTGCATCGTCGCCATTCCTTTTTTGTGGGCGGGTTTTGGTTTTATGATTAAAAAAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCAc                                                     <  1:2210081/150‑1 (MQ=255)
                                                                                                    aCTGCATCGTCGCATTTCCGATATTGTTCGCGCGATTTCGGTTTATGATTAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCg                                                  <  2:2504134/150‑1 (MQ=255)
                                                                                                      tGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTg                                                >  2:284934/1‑150 (MQ=255)
                                                                                                        aaTCGTCGCCATTCCTATTTTGTTCGCGCGATTTGGGTTTATGATTAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGAc                                              <  1:3605485/149‑1 (MQ=255)
                                                                                                        aaTCGTCGCAATTCCTATTTTGTTCGCGCTTTTTCGTTTTATTATTAAAATAACCGACTCTTTGTCACCTCCGATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGAc                                              <  1:2278654/149‑1 (MQ=255)
                                                                                                         aTCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACg                                             >  1:1204666/1‑150 (MQ=255)
                                                                                                         aTCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACg                                             >  1:2702523/1‑150 (MQ=255)
                                                                                                         aTCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACg                                             >  1:3099126/1‑150 (MQ=255)
                                                                                                            gtcgCCATTCCGATATTGTTCGCGCGATTTGGTTTTATTATTAAAATAACCGACTCTTCGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCg                                          <  1:2805238/150‑1 (MQ=255)
                                                                                                            gtcgCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCg                                          >  1:2464426/1‑150 (MQ=255)
                                                                                                              cgcgATTCCGATTTTGTTCGCGCGATTTCGTTTTATTATCAAAAAAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGat                                        <  1:2657969/150‑1 (MQ=255)
                                                                                                              cgCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGat                                        >  1:2414335/1‑150 (MQ=255)
                                                                                                              cgCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGat                                        >  1:4126039/1‑150 (MQ=255)
                                                                                                              cgCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGat                                        >  2:2974116/1‑150 (MQ=255)
                                                                                                                ccATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGatat                                      >  1:3627618/1‑150 (MQ=255)
                                                                                                                 cATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGAtatt                                     >  2:425896/1‑150 (MQ=255)
                                                                                                                   ttCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGAtattat                                   >  2:310268/1‑150 (MQ=255)
                                                                                                                     ccGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGa                                 >  2:841739/1‑150 (MQ=255)
                                                                                                                       gATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGAtc                               >  1:3327717/1‑150 (MQ=255)
                                                                                                                         tatTGTTCGCGCGATTTCGGTTTATGATTAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGAtcct                             <  2:1568838/150‑1 (MQ=255)
                                                                                                                          atTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGAtcctc                            <  2:3591309/150‑1 (MQ=255)
                                                                                                                           ttGTTCGCGCGTTTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGAtcctcc                           <  2:4022420/150‑1 (MQ=255)
                                                                                                                           ttGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGAtcctcc                           >  1:811535/1‑150 (MQ=255)
                                                                                                                             gTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGc                         >  2:2010096/1‑150 (MQ=255)
                                                                                                                             gTTCGCGCGATTTCGGTTTATGATGAAAATAACAGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGc                         >  2:2008890/1‑150 (MQ=255)
                                                                                                                                  cgcgTTTTCGTTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGCACGcc                    <  2:1029246/150‑1 (MQ=255)
                                                                                                                                  cgcgATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGCACGcc                    >  2:3442374/1‑150 (MQ=255)
                                                                                                                                      aTTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGAACCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGCACGCCGatt                >  2:4299381/1‑150 (MQ=255)
                                                                                                                                      aTTTCGGTTTATGATCAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGCACGCCGatt                <  2:2282935/150‑1 (MQ=255)
                                                                                                                                       tttCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGCACGCCGatta               >  1:3524264/1‑150 (MQ=255)
                                                                                                                                         tgggTTTATTATCAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGCACGCCGattatt             <  1:2924811/148‑1 (MQ=255)
                                                                                                                                           gttttATTATTAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGCACGCCGATTATTTc           <  1:3779723/148‑1 (MQ=255)
                                                                                                                                           ggTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGCACGCCGATTATTTc           >  1:1005008/1‑150 (MQ=255)
                                                                                                                                              ttATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGCACGCCGATTATTTCTga        >  2:667314/1‑150 (MQ=255)
                                                                                                                                                atgatgAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGCACGCCGATTATTTCTgaga      >  1:1720084/1‑150 (MQ=255)
                                                                                                                                                atgatgAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGCACGCCGATTATTTCTgaga      >  1:1760681/1‑150 (MQ=255)
                                                                                                                                                atgatgAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGCACGCCGATTATTTCTgaga      >  2:760415/1‑150 (MQ=255)
                                                                                                                                                 tgatgaAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGCACGCCGATTATTTCTGagag     >  1:2359231/1‑150 (MQ=255)
                                                                                                                                                    tgaAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGCACTCCGATTATTTCTGAGAGTTa  >  1:1345997/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGAGCCTGCAAAATCGCGAATCTATCGGTCAGTTAATAAGCGTACTGCATCGTCGCCATTCCGATATTGTTCGCGCGATTTCGGTTTATGATGAAAATAACCGACTCTTTGTCACCTCCAATTTTCATCTTGATCCCTCATCAATGCAGCTCGGCAGCAACGTGCCGTTTCCTCGCCAGCTCACTGTCACTCGTGACGGCGATATTATGATCCTCCGCACGCCGATTATTTCTGAGAGTTA  >  CP009273/2908537‑2908834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑