Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,055,069 C→A 21.2% V287V (GTC→GTA)  scpA → methylmalonyl‑CoA mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,055,0690CA21.2% 759.8 / 163.8 310V287V (GTC→GTA) scpAmethylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand):  ref base C (137/106);  new base A (30/37);  total (167/143)
Fisher's exact test for biased strand distribution p-value = 9.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 315 total aligned reads displayed.

GGTGCCAACTGCGTGCAGCAGGTAGCATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCAC  >  CP009273/3054920‑3055218
                                                                                                                                                     |                                                                                                                                                     
ggTGCCAACTGCGTGCAGCAGGTAGCATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTc                                                                                                                                                       <  1:341699/150‑1 (MQ=255)
 gTGCCAACTGCGTGCAGCAGGTAGCATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCg                                                                                                                                                      <  2:222076/150‑1 (MQ=255)
   gCCAACTGCGTGCAGCAGGTAGCATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGcc                                                                                                                                                    >  1:4145263/1‑150 (MQ=255)
    ccAACTGCGTGCAGCAGGTAGCATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCa                                                                                                                                                   <  1:3865646/150‑1 (MQ=255)
     cAACTGCGTGCAGCAGGTAGCATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCAt                                                                                                                                                  <  2:2932787/150‑1 (MQ=255)
       aCTGCGTGCAGCAGGTAGCATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGt                                                                                                                                                >  2:112978/1‑150 (MQ=255)
        cTGCGTGCAGCAGGTAGCATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTAGCCATGtt                                                                                                                                               <  1:3541662/150‑1 (MQ=255)
         tGCGTGCAGCAGGTAGCATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTtg                                                                                                                                              <  1:2817430/150‑1 (MQ=255)
         tGCGTGCAGCAGGTAGCATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTtg                                                                                                                                              <  1:537130/150‑1 (MQ=255)
              gcagcaGGTAGCATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTtgcgtgc                                                                                                                                         <  2:3485658/150‑1 (MQ=255)
               cagcagGTAGCATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTtgcgtgcg                                                                                                                                        <  1:1979881/150‑1 (MQ=255)
               cagcagGTAGCATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTAGCCATGTtgcgtgcg                                                                                                                                        <  1:4089896/150‑1 (MQ=255)
               cagcagGTAGCATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTAGCCATGTtgcgtgcg                                                                                                                                        <  1:32763/150‑1 (MQ=255)
                 gcagGTAGCATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGc                                                                                                                                      >  1:616253/1‑150 (MQ=255)
                     gTAGCATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGt                                                                                                                                  >  2:4288659/1‑150 (MQ=255)
                         cATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGttatt                                                                                                                              <  2:1632713/150‑1 (MQ=255)
                           tttACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGttattta                                                                                                                            <  1:358090/150‑1 (MQ=255)
                               cgctcgctGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGa                                                                                                                        <  2:4104833/150‑1 (MQ=255)
                               cgctcgctGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTAGCCATGTTGCGTGCGGCACGTTATTTATGGa                                                                                                                        >  2:2013893/1‑150 (MQ=255)
                                    gctGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTAGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGaa                                                                                                                   >  1:517190/1‑150 (MQ=255)
                                       gATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTTCGTGCGGCACGTTATTTATGGAGCGAAGCg                                                                                                                >  1:4027372/1‑150 (MQ=255)
                                         tGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGt                                                                                                              <  2:3769247/150‑1 (MQ=255)
                                         tGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGt                                                                                                              >  2:4042291/1‑150 (MQ=255)
                                         tGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGt                                                                                                              >  2:3045585/1‑150 (MQ=255)
                                          gggATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTc                                                                                                             <  1:2142709/150‑1 (MQ=255)
                                                 aGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGAt                                                                                                      <  1:2291154/150‑1 (MQ=255)
                                                   tACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCCTCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGAttt                                                                                                    >  2:2730949/1‑150 (MQ=255)
                                                   tACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGAttt                                                                                                    <  2:2524327/150‑1 (MQ=255)
                                                   tACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCGTCTTCGGCATCGGCATGGATCTGTTTATGAACGGCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGAttt                                                                                                    >  1:2301952/1‑150 (MQ=255)
                                                       tCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGgcg                                                                                                >  2:3639766/1‑150 (MQ=255)
                                                        cAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGgcgc                                                                                               >  1:3146886/1‑150 (MQ=255)
                                                           agcagcAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGcaca                                                                                            <  2:2569971/150‑1 (MQ=255)
                                                           agcagcAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCGGTTTATGAACGTCGCCATGTTGCGTGCGGCACGGTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGcaca                                                                                            >  1:3696173/1‑150 (MQ=255)
                                                             cagcaATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTGTTAGTCGGCCTCGGCATTGGTCTGTTTATGAACGTCGCCCTGTTGCGTGCGGCACGTTATTTATTGAGCGAAGCGGTCAGTGGATTTGGCGCACAgg                                                                                          >  1:1837551/1‑150 (MQ=255)
                                                              agcaATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTTGCGCACAGGa                                                                                         >  1:255329/1‑150 (MQ=255)
                                                              agcaATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTAGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGa                                                                                         >  1:4260248/1‑150 (MQ=255)
                                                                 aaTCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGAccc                                                                                      >  1:2893065/1‑150 (MQ=255)
                                                                  aTCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCg                                                                                     <  1:4246716/150‑1 (MQ=255)
                                                                      ctGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAAt                                                                                 >  2:3956353/1‑150 (MQ=255)
                                                                        gCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCa                                                                               <  2:3573690/150‑1 (MQ=255)
                                                                        gCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCa                                                                               <  1:169025/150‑1 (MQ=255)
                                                                        gCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCa                                                                               <  1:724609/150‑1 (MQ=255)
                                                                         ccGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTGCTTCTTCGGCCTCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATTGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGaaaaaac                                                                              >  1:10397/1‑146 (MQ=255)
                                                                          cGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACt                                                                             >  1:1224870/1‑150 (MQ=255)
                                                                            gACTGAAAATTGATGACATCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTgg                                                                           >  1:199504/1‑150 (MQ=255)
                                                                             aCTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTAGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGgc                                                                          <  2:3222006/150‑1 (MQ=255)
                                                                              cTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAAAGTCGCCATGTTGCGTGCGGCACCTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACAGgcg                                                                         >  2:393218/1‑150 (MQ=255)
                                                                               tGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTATTCGGCATCGGCATGGATCTGTTTATGAACGTAGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTTGCGCACAGGACCCGAAATCACAGgcgc                                                                        >  1:1518929/1‑150 (MQ=255)
                                                                                gAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCt                                                                       >  2:2560258/1‑150 (MQ=255)
                                                                                gAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCt                                                                       >  2:132178/1‑150 (MQ=255)
                                                                                 aaaaTTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTg                                                                      <  2:1484983/150‑1 (MQ=255)
                                                                                 aaaaTTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTg                                                                      <  1:1317799/150‑1 (MQ=255)
                                                                                      tgatgaCTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTAc                                                                 >  2:1920561/1‑150 (MQ=255)
                                                                                        atgaCTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGAACTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGCCCCGCAATCACTGGCGCTGCGTAccc                                                               >  2:3338433/1‑150 (MQ=255)
                                                                                          gaCTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTAGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCAc                                                             >  1:666646/1‑150 (MQ=255)
                                                                                            cTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTg                                                           >  2:786939/1‑150 (MQ=255)
                                                                                            cTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTg                                                           >  1:916908/1‑150 (MQ=255)
                                                                                            cTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTAGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCCCAGGACCCGACATCACTGGCGCTGCGTACCCACTg                                                           >  2:1837707/1‑150 (MQ=255)
                                                                                            cTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCACGGATCTGTTTATGAACGTAGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTg                                                           <  2:18970/150‑1 (MQ=255)
                                                                                                gCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAg                                                       <  1:121066/150‑1 (MQ=255)
                                                                                                gCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTAGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTTGCGCACAGGACCCGAAATCACAGGCGCTGCGTACCCACTGCCAg                                                       >  2:686212/1‑150 (MQ=255)
                                                                                                   cctcGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGAcc                                                    <  2:2374983/150‑1 (MQ=255)
                                                                                                        ccTGTCGTTCATCTTAGGCATCGGCATGGATCAGTTTATGAACGTCGCCAAGTTTCGTGCGGCACGTTATTTATTGAGCGAAGCGGTCCGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACAGCCAGACCACAgg                                               >  2:251446/1‑150 (MQ=255)
                                                                                                          tGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCt                                             >  2:2460173/1‑150 (MQ=255)
                                                                                                             cGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGa                                          >  1:2538600/1‑150 (MQ=255)
                                                                                                             cGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGa                                          >  1:823007/1‑150 (MQ=255)
                                                                                                                tcttcttcGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGcc                                       >  1:2669660/1‑150 (MQ=255)
                                                                                                                 cttcttcGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCct                                      >  1:2967694/1‑150 (MQ=255)
                                                                                                                 cttcttcGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCct                                      >  2:560418/1‑150 (MQ=255)
                                                                                                                  ttcttcGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCctg                                     >  2:472493/1‑150 (MQ=255)
                                                                                                                  ttcttcGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCctg                                     <  1:2351198/150‑1 (MQ=255)
                                                                                                                        ggcatcggcatGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGaa                               >  2:3154421/1‑150 (MQ=255)
                                                                                                                         gcatcggcatGGATCTGTTTATGAACGTAGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAAc                              >  1:992846/1‑150 (MQ=255)
                                                                                                                          catcggcatGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACa                             <  1:349956/150‑1 (MQ=255)
                                                                                                                          catcggcatGGATCTGTTTATGAACGTAGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACa                             <  1:932424/150‑1 (MQ=255)
                                                                                                                            tcggcatGGATCTGTTTATGAACGTAGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAgg                           <  1:2198494/150‑1 (MQ=255)
                                                                                                                              ggcatGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGAt                         >  1:2025199/1‑150 (MQ=255)
                                                                                                                               gcatGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATc                        <  2:3022572/150‑1 (MQ=255)
                                                                                                                                catGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATcc                       <  1:954427/150‑1 (MQ=255)
                                                                                                                                catGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATcc                       <  1:2596744/150‑1 (MQ=255)
                                                                                                                                  tGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGt                     >  2:1596663/1‑150 (MQ=255)
                                                                                                                                  tGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGt                     >  1:3942156/1‑150 (MQ=255)
                                                                                                                                   ggATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGta                    >  2:2876761/1‑150 (MQ=255)
                                                                                                                                   ggATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGta                    >  1:1627911/1‑150 (MQ=255)
                                                                                                                                   ggATCTGTTTATGAACGTAGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGta                    >  2:3717361/1‑150 (MQ=255)
                                                                                                                                    gATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGtat                   >  2:1561530/1‑150 (MQ=255)
                                                                                                                                    gATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGtat                   >  2:2319222/1‑150 (MQ=255)
                                                                                                                                     aTCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGtata                  >  1:1263651/1‑150 (MQ=255)
                                                                                                                                      tCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATaa                 <  2:2850106/150‑1 (MQ=255)
                                                                                                                                       cTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATaac                <  1:2699797/150‑1 (MQ=255)
                                                                                                                                       cTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATaac                >  2:4326179/1‑150 (MQ=255)
                                                                                                                                        tGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATaaca               <  1:3424666/150‑1 (MQ=255)
                                                                                                                                        tGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATaaca               <  2:3692263/150‑1 (MQ=255)
                                                                                                                                        tGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATaaca               <  2:1697772/150‑1 (MQ=255)
                                                                                                                                        tGTTTATGAACGTCGCCATGGTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATaaca               <  2:3412240/150‑1 (MQ=255)
                                                                                                                                          tttATGAACGTAGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATaacaac             <  2:2030910/150‑1 (MQ=255)
                                                                                                                                                aaCGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATc       >  1:2502090/1‑150 (MQ=255)
                                                                                                                                                aaCGTAGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATc       <  1:9429/150‑1 (MQ=255)
                                                                                                                                                 aCGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATcc      <  1:2786054/150‑1 (MQ=255)
                                                                                                                                                  cGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCg     <  2:3251385/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGTGCCAACTGCGTGCAGCAGGTAGCATTTACGCTCGCTGATGGGATTGAGTACATCAAAGCAGCAATCTCTGCCGGACTGAAAATTGATGACTTCGCTCCTCGCCTGTCGTTCTTCTTCGGCATCGGCATGGATCTGTTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCAC  >  CP009273/3054920‑3055218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑