Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,092,496 C→A 6.9% V143V (GTG→GTT)  yggM ← DUF1202 family putative secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,092,4960CA 6.9% 978.0 / 35.1 259V143V (GTG→GTT) yggMDUF1202 family putative secreted protein
Reads supporting (aligned to +/- strand):  ref base C (129/112);  new base A (8/10);  total (137/122)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 266 total aligned reads displayed.

TTTCCCCAATATGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATCGCCGAGAATTTTACCGGTTTATC  >  CP009273/3092347‑3092645
                                                                                                                                                     |                                                                                                                                                     
  tcaccAATATGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCAc                                                                                                                                                     <  1:472051/147‑1 (MQ=255)
   ccccAATATGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACg                                                                                                                                                    <  1:333758/150‑1 (MQ=255)
    cccAATATGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGa                                                                                                                                                   >  2:1417778/1‑150 (MQ=255)
     ccAATATGCATCCGCTGCTTTCTGCGCTGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGAt                                                                                                                                                  <  1:2417725/150‑1 (MQ=255)
      cAATATGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATc                                                                                                                                                 >  2:703078/1‑150 (MQ=255)
          atGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTAACGATCAGat                                                                                                                                             >  2:1842965/1‑150 (MQ=255)
             cATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGAtatt                                                                                                                                          <  2:3068071/150‑1 (MQ=255)
              aTCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGAtattt                                                                                                                                         >  2:2204877/1‑150 (MQ=255)
                ccGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGAtatttat                                                                                                                                       <  1:675550/150‑1 (MQ=255)
                ccGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGAtatgtat                                                                                                                                       >  2:2216249/1‑150 (MQ=255)
                  gctgctTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATttatta                                                                                                                                     >  1:3668878/1‑150 (MQ=255)
                   ctgctTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTtattat                                                                                                                                    >  2:1050300/1‑150 (MQ=255)
                    tgctTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTattatt                                                                                                                                   >  1:397499/1‑150 (MQ=255)
                     gctTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTc                                                                                                                                  <  2:2732188/150‑1 (MQ=255)
                     gctTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTc                                                                                                                                  <  2:4317878/150‑1 (MQ=255)
                        ttCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGtt                                                                                                                               <  2:4249746/150‑1 (MQ=255)
                         tCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTAACGATCAGATATTTATTATTCGttt                                                                                                                              <  1:1146057/150‑1 (MQ=255)
                           tGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTg                                                                                                                            <  1:2912714/150‑1 (MQ=255)
                           tGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTg                                                                                                                            <  1:2486184/150‑1 (MQ=255)
                              gccgccACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGata                                                                                                                         <  1:1028310/150‑1 (MQ=255)
                              gccgccACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGata                                                                                                                         <  1:987609/150‑1 (MQ=255)
                                 gccACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATAtcg                                                                                                                      >  2:3702420/1‑150 (MQ=255)
                                 gccACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTAACGATCAGATATTTATTATTCGTTTTGATAtcg                                                                                                                      >  1:2797958/1‑150 (MQ=255)
                                  ccaccaTTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATAtcgt                                                                                                                     >  1:2594035/1‑150 (MQ=255)
                                    accaTTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATAtcgtcg                                                                                                                   >  2:1188503/1‑150 (MQ=255)
                                    accaTTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATAtcgtcg                                                                                                                   <  2:683295/150‑1 (MQ=255)
                                       aTTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGacc                                                                                                                >  1:166994/1‑150 (MQ=255)
                                            cTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCg                                                                                                           >  1:2711659/1‑150 (MQ=255)
                                            cTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCg                                                                                                           >  2:3027885/1‑150 (MQ=255)
                                            cTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTAACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCg                                                                                                           <  2:2639312/150‑1 (MQ=255)
                                                    gcgcTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGAt                                                                                                   <  2:357634/150‑1 (MQ=255)
                                                      gcTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCg                                                                                                 >  2:829685/1‑150 (MQ=255)
                                                        tttCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCgct                                                                                               >  1:2764464/1‑150 (MQ=255)
                                                          tCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCGAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATAGTCTACCACCCGCCCACGATCgctgg                                                                                             >  1:2626681/1‑150 (MQ=255)
                                                             cTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCgctggctg                                                                                          >  2:554587/1‑150 (MQ=255)
                                                             cTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCgctggctg                                                                                          >  1:1398762/1‑150 (MQ=255)
                                                              ttCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTgc                                                                                         <  2:1977766/150‑1 (MQ=255)
                                                              ttCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTgc                                                                                         <  2:1977884/150‑1 (MQ=255)
                                                              ttCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTgc                                                                                         <  2:1396251/150‑1 (MQ=255)
                                                               tCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTgcc                                                                                        >  1:2008152/1‑150 (MQ=255)
                                                                ccTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTgccg                                                                                       <  1:2261881/150‑1 (MQ=255)
                                                                ccTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTgccg                                                                                       <  2:485589/150‑1 (MQ=255)
                                                                ccTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTgccg                                                                                       >  2:1266008/1‑150 (MQ=255)
                                                                     tCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGa                                                                                  >  2:2399241/1‑150 (MQ=255)
                                                                      ccAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGaa                                                                                 >  1:2711031/1‑150 (MQ=255)
                                                                              ggAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTaa                                                                         <  1:1833660/150‑1 (MQ=255)
                                                                                aaTCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTAACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTaaaa                                                                       >  1:3456150/1‑150 (MQ=255)
                                                                                 aTCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTAACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAg                                                                      >  1:1825134/1‑150 (MQ=255)
                                                                                  tCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGt                                                                     >  1:3519429/1‑150 (MQ=255)
                                                                                     aGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCa                                                                  >  2:3623041/1‑150 (MQ=255)
                                                                                     aGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCa                                                                  <  2:290953/150‑1 (MQ=255)
                                                                                      gTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCAc                                                                 >  2:2091941/1‑150 (MQ=255)
                                                                                      gTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCAc                                                                 >  1:1097860/1‑150 (MQ=255)
                                                                                        tcttctGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCg                                                               <  1:1229132/150‑1 (MQ=255)
                                                                                            cttgttATTCAACGAAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGTcca                                                           <  2:4355565/145‑1 (MQ=255)
                                                                                            ctGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGAcca                                                           >  2:176654/1‑150 (MQ=255)
                                                                                               ggTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGAccagcc                                                        <  1:262719/150‑1 (MQ=255)
                                                                                                gTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGAccagcca                                                       >  2:1719754/1‑150 (MQ=255)
                                                                                                 tATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGAccagccag                                                      >  2:2156614/1‑150 (MQ=255)
                                                                                                      aaCGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGAcgcg                                                 <  2:3249425/150‑1 (MQ=255)
                                                                                                      aaCGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGAcgcg                                                 <  2:1054647/150‑1 (MQ=255)
                                                                                                      aaCGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGAcgcg                                                 <  2:749192/150‑1 (MQ=255)
                                                                                                      aaCGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGAcgcg                                                 >  1:2057161/1‑150 (MQ=255)
                                                                                                        cGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCggg                                               <  1:1625490/150‑1 (MQ=255)
                                                                                                        cGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCggg                                               <  1:3888999/150‑1 (MQ=255)
                                                                                                        cGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCggg                                               <  1:3038884/150‑1 (MQ=255)
                                                                                                         gCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGc                                              <  1:3423348/150‑1 (MQ=255)
                                                                                                          cAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTAACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCg                                             >  2:616131/1‑150 (MQ=255)
                                                                                                          cAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTAACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCg                                             >  2:3083584/1‑150 (MQ=255)
                                                                                                             aTTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTc                                          >  2:3349720/1‑150 (MQ=255)
                                                                                                                 aaGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCttt                                      >  1:45991/1‑150 (MQ=255)
                                                                                                                 aaGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCttt                                      >  2:3129042/1‑150 (MQ=255)
                                                                                                                 aaGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTGCCttt                                      >  1:4198182/1‑150 (MQ=255)
                                                                                                                     tGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTg                                  >  2:3809079/1‑150 (MQ=255)
                                                                                                                       aCTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGt                                >  1:1706509/1‑150 (MQ=255)
                                                                                                                        cTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTTCTGGtt                               >  1:12826/1‑150 (MQ=255)
                                                                                                                         tAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTa                              >  1:1116878/1‑150 (MQ=255)
                                                                                                                         tAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTa                              <  2:4142935/150‑1 (MQ=255)
                                                                                                                         tAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTGCCTTTACTGGTTa                              >  1:2190330/1‑150 (MQ=255)
                                                                                                                            aGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACa                           <  1:4980/150‑1 (MQ=255)
                                                                                                                            aGCGATAATTGAAATCTTCGCTATTAACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACa                           >  2:3948750/1‑150 (MQ=255)
                                                                                                                               gATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTAATGGTTAACATCg                        >  1:3876072/1‑150 (MQ=255)
                                                                                                                               gATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCGTTACTGGTTAACATCg                        >  1:1008462/1‑150 (MQ=255)
                                                                                                                                aTAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATCGc                       >  1:2107461/1‑150 (MQ=255)
                                                                                                                                     tGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATCGCCgaga                  >  1:2079623/1‑150 (MQ=255)
                                                                                                                                     tGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATCGCCgaga                  >  2:4090337/1‑150 (MQ=255)
                                                                                                                                       aaaTCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAATTTCACCGACCAGCCAGACGCGGGCGTTGCTTTTCTGGTTTTCAAAGCCGCGAAt                >  1:2905217/1‑150 (MQ=255)
                                                                                                                                        aaTCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATCGCCGAGAAtt               <  2:203289/150‑1 (MQ=255)
                                                                                                                                          tCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATCGCCGAGAAtttt             <  2:1583519/150‑1 (MQ=255)
                                                                                                                                           cTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATCGCCGAGAATTTTa            >  1:2653301/1‑150 (MQ=255)
                                                                                                                                             tCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATCGCCGAGAATTTTAcc          <  2:4206837/150‑1 (MQ=255)
                                                                                                                                             tCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATCGCCGAGAATTTTAcc          <  2:1790149/150‑1 (MQ=255)
                                                                                                                                               gCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATCGCCGAGAATTTTACCgg        <  2:3409398/150‑1 (MQ=255)
                                                                                                                                               gCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATCGCCGAGAATTTTACCgg        >  2:3105990/1‑150 (MQ=255)
                                                                                                                                                cTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATCGCCGAGAATTTTACCGGt       <  1:933463/150‑1 (MQ=255)
                                                                                                                                                 tATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATCGCCGAGAATTTTACCGGtt      <  2:1097903/150‑1 (MQ=255)
                                                                                                                                                   ttCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATCGCCGAGAATTTTACCGGTTTa    >  2:2674226/1‑150 (MQ=255)
                                                                                                                                                     cACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATCGCCGAGAATTTTACCGGTTTAta  >  1:4287718/1‑149 (MQ=255)
                                                                                                                                                     cACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATAGCCGAGAATTTTACCGGTTTATc  >  1:3890212/1‑150 (MQ=255)
                                                                                                                                                     cACGATCAGATATTAATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATCGCCGAGAATTTTACCGGTTTATc  <  2:2090195/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTCCCCAATATGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGATAATTGAAATCTTCGCTATTCACGATCAGATATTTATTATTCGTTTTGATATCGTCGACCACCCGCCCACGATCGCTGGCTGCCGCCTGAAAAGAGTAAAAGTTCACCGACCAGCCAGACGCGGGCGTTCCTTTACTGGTTAACATCGCCGAGAATTTTACCGGTTTATC  >  CP009273/3092347‑3092645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑