Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,132,601 T→G 14.1% I172M (ATT→ATG)  yghU → putative S‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,132,6010TG14.1% 640.5 / 110.7 255I172M (ATT→ATG) yghUputative S‑transferase
Reads supporting (aligned to +/- strand):  ref base T (104/115);  new base G (14/22);  total (118/137)
Fisher's exact test for biased strand distribution p-value = 3.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

Only 100 of 258 total aligned reads displayed.

CTTTATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGGTGTTAGG  >  CP009273/3132452‑3132750
                                                                                                                                                     |                                                                                                                                                     
cTTTATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGAtt                                                                                                                                                       <  1:813144/150‑1 (MQ=255)
   tATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAg                                                                                                                                                    >  1:2939516/1‑150 (MQ=255)
     tCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTa                                                                                                                                                  <  1:2518498/150‑1 (MQ=255)
         gCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGcc                                                                                                                                              <  2:742093/150‑1 (MQ=255)
          cGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCa                                                                                                                                             >  2:3695478/1‑150 (MQ=255)
           ggAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCAt                                                                                                                                            >  1:401886/1‑150 (MQ=255)
           ggAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCAt                                                                                                                                            <  2:2861902/150‑1 (MQ=255)
            gagaAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATc                                                                                                                                           <  2:1765902/150‑1 (MQ=255)
            gagaAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATc                                                                                                                                           <  1:182308/150‑1 (MQ=255)
             agaAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCa                                                                                                                                          >  1:698913/1‑150 (MQ=255)
                aaTTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAAcc                                                                                                                                       >  2:461463/1‑150 (MQ=255)
                     ggCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCttt                                                                                                                                  <  2:86132/150‑1 (MQ=255)
                        tACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTAcc                                                                                                                               <  1:404903/150‑1 (MQ=255)
                        tACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTAcc                                                                                                                               <  1:373966/150‑1 (MQ=255)
                          cTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCAt                                                                                                                             <  2:3840055/150‑1 (MQ=255)
                               tGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAg                                                                                                                        >  2:2911698/1‑150 (MQ=255)
                                  cGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTACAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCa                                                                                                                     >  2:1516461/1‑150 (MQ=255)
                                     aGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAAc                                                                                                                  <  1:3925516/150‑1 (MQ=255)
                                     aGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAAc                                                                                                                  <  1:2416731/150‑1 (MQ=255)
                                       gATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGt                                                                                                                >  2:4269119/1‑150 (MQ=255)
                                        aTTTGGCAAAGCGTACTGAAACGATGAACGGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTc                                                                                                               <  2:1184432/150‑1 (MQ=255)
                                          ttGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTg                                                                                                             >  2:3898268/1‑150 (MQ=255)
                                          ttGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTg                                                                                                             <  2:1861553/150‑1 (MQ=255)
                                            ggCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCt                                                                                                           >  1:1292712/1‑150 (MQ=255)
                                             gCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTc                                                                                                          <  1:1957777/150‑1 (MQ=255)
                                                aaGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGAc                                                                                                       >  1:487504/1‑150 (MQ=255)
                                                     tACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCt                                                                                                  >  1:1204486/1‑150 (MQ=255)
                                                      aCTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTg                                                                                                 >  2:2274657/1‑150 (MQ=255)
                                                      aCTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTg                                                                                                 <  1:3477200/150‑1 (MQ=255)
                                                      aCTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTg                                                                                                 <  1:1936691/150‑1 (MQ=255)
                                                         gAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGAt                                                                                              >  1:124716/1‑150 (MQ=255)
                                                           aaCGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATaa                                                                                            >  2:3818114/1‑150 (MQ=255)
                                                              gATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCa                                                                                         >  2:1410609/1‑150 (MQ=255)
                                                                tGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAAc                                                                                       >  1:4219733/1‑150 (MQ=255)
                                                                tGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAAc                                                                                       <  2:2056029/150‑1 (MQ=255)
                                                                tGAACTGGCTGTTCTGGTCACAGGGCGCGGCACCGGTCCTCGGCGGTGGTTTTGGTCACTATTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAAc                                                                                       <  2:3255771/150‑1 (MQ=255)
                                                                 gAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACt                                                                                      <  2:3079755/150‑1 (MQ=255)
                                                                 gAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACt                                                                                      >  1:1978777/1‑150 (MQ=255)
                                                                  aaCTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTg                                                                                     <  2:401886/150‑1 (MQ=255)
                                                                  aaCTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTg                                                                                     >  1:3965335/1‑150 (MQ=255)
                                                                   aCTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTgg                                                                                    >  1:3980819/1‑150 (MQ=255)
                                                                    cTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGgc                                                                                   >  1:871673/1‑150 (MQ=255)
                                                                    cTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGgc                                                                                   >  2:3049645/1‑150 (MQ=255)
                                                                     tGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGgcg                                                                                  >  2:1795235/1‑150 (MQ=255)
                                                                     tGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGgcg                                                                                  >  1:1752828/1‑150 (MQ=255)
                                                                     tGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGgcg                                                                                  >  1:3810182/1‑150 (MQ=255)
                                                                       gCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCgca                                                                                <  1:3517736/150‑1 (MQ=255)
                                                                       gCTGTTCTGGTTACAGGGCGCGGCACCGGTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCgca                                                                                <  1:3534477/150‑1 (MQ=255)
                                                                         tGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCgcagc                                                                              >  1:1883781/1‑150 (MQ=255)
                                                                             cTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATaa                                                                          >  2:1351842/1‑150 (MQ=255)
                                                                                gTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGtt                                                                       <  2:2255971/150‑1 (MQ=255)
                                                                                gTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGtt                                                                       <  2:2256048/150‑1 (MQ=255)
                                                                                gTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGtt                                                                       <  2:83687/150‑1 (MQ=255)
                                                                                 ttACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGttt                                                                      <  1:1123677/150‑1 (MQ=255)
                                                                                 ttACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGttt                                                                      <  2:330441/150‑1 (MQ=255)
                                                                                    cAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTttgtt                                                                   <  1:1070959/150‑1 (MQ=255)
                                                                                    cAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTttgtt                                                                   <  2:59825/150‑1 (MQ=255)
                                                                                       ggCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCg                                                                <  1:1384860/150‑1 (MQ=255)
                                                                                          gcgGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGc                                                             <  1:2209652/150‑1 (MQ=255)
                                                                                          gcgGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGc                                                             <  1:1678444/150‑1 (MQ=255)
                                                                                              cACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATg                                                         <  1:3334057/150‑1 (MQ=255)
                                                                                                  gTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTa                                                     <  2:416144/150‑1 (MQ=255)
                                                                                                   ttCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTac                                                    >  2:1199747/1‑150 (MQ=255)
                                                                                                     ccTCGGCGGTGGTTTTGGTCACTTTTACCATTACTCACCGGTAAAGATGGAGTACGCCAACAACCGCTATACCAAGGAAGCCAAACGTCTGCTCGACGAGCTGGATAAGAAAATGCCGCAGAATAAGTTTGTTGCGGGAGATGAGTaaac                                                  >  1:3123099/1‑150 (MQ=255)
                                                                                                       tCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCa                                                >  1:584912/1‑150 (MQ=255)
                                                                                                         ggcggTGGTTTTGGTCACTTTTACCCTTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCAtt                                              <  2:1673491/150‑1 (MQ=255)
                                                                                                           cggTGGTTTTGGTCACTTTTACCATTACGCACCTGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGc                                            <  2:4081480/150‑1 (MQ=255)
                                                                                                               ggtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGat                                        >  2:494236/1‑150 (MQ=255)
                                                                                                               ggtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGat                                        >  1:4184076/1‑150 (MQ=255)
                                                                                                                gtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCAATCAACCGCTTTACCATGGAAGCCAAACGTCTGCACGACTTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGAGATGAGTACACCATTGCGGata                                       >  1:3055133/1‑150 (MQ=255)
                                                                                                                gtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGata                                       >  1:1165469/1‑150 (MQ=255)
                                                                                                                 ttttGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGatat                                      <  1:3356692/150‑1 (MQ=255)
                                                                                                                 ttttGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGatat                                      <  2:4212080/150‑1 (MQ=255)
                                                                                                                  tttGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATg                                     <  1:1816880/150‑1 (MQ=255)
                                                                                                                      gTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGa                                 >  2:3499272/1‑150 (MQ=255)
                                                                                                                       tCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGAt                                >  2:591582/1‑150 (MQ=255)
                                                                                                                        cACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCAGAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGAtt                               >  2:1155848/1‑150 (MQ=255)
                                                                                                                         aCTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGAttt                              <  2:2508442/150‑1 (MQ=255)
                                                                                                                             ttACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGcc                          <  2:1589144/150‑1 (MQ=255)
                                                                                                                               aCCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGt                        >  2:2326112/1‑150 (MQ=255)
                                                                                                                               aCCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGt                        >  1:1541074/1‑150 (MQ=255)
                                                                                                                                ccATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTg                       >  2:3992088/1‑150 (MQ=255)
                                                                                                                                  aTTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGTATAAGCAACTGGCGCAGCATAAGTTTGTTGAGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGt                     >  1:19197/1‑150 (MQ=255)
                                                                                                                                  aTTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGt                     <  2:2408980/150‑1 (MQ=255)
                                                                                                                                    tACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGttt                   >  2:3111684/1‑150 (MQ=255)
                                                                                                                                    tACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGttt                   <  2:2806886/150‑1 (MQ=255)
                                                                                                                                      cGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTGTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTgg                 >  2:3575680/1‑150 (MQ=255)
                                                                                                                                       gCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGc                <  1:2782019/150‑1 (MQ=255)
                                                                                                                                        cACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCa               >  1:3026130/1‑150 (MQ=255)
                                                                                                                                         aCCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCaa              >  2:644757/1‑150 (MQ=255)
                                                                                                                                         aCCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCaa              >  2:1851114/1‑150 (MQ=255)
                                                                                                                                         aCCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATGTGGCCGTGGTTTGGCaa              >  2:3596156/1‑150 (MQ=255)
                                                                                                                                            ggTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgt           >  2:3730652/1‑150 (MQ=255)
                                                                                                                                            ggTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgt           <  1:1143327/150‑1 (MQ=255)
                                                                                                                                             gTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgtg          <  2:1135715/150‑1 (MQ=255)
                                                                                                                                               aaaGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgtggt        >  1:2285586/1‑150 (MQ=255)
                                                                                                                                               aaaGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgtggt        <  1:2327407/150‑1 (MQ=255)
                                                                                                                                                  gATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGGTGtt     <  1:2707914/150‑1 (MQ=255)
                                                                                                                                                   aTTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGGTGTTa    <  1:4178983/150‑1 (MQ=255)
                                                                                                                                                   aTTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGGTGTTa    <  1:3034809/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTTATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGGTGTTAGG  >  CP009273/3132452‑3132750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑