Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,142,668 G→T 41.5% L55I (CTC→ATC)  yqhA ← UPF0114 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,142,6680GT41.5% 224.3 / 290.2 241L55I (CTC→ATC) yqhAUPF0114 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (80/61);  new base T (44/56);  total (124/117)
Fisher's exact test for biased strand distribution p-value = 6.69e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 246 total aligned reads displayed.

GACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCATTTTCA  >  CP009273/3142520‑3142816
                                                                                                                                                    |                                                                                                                                                    
gACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAgg                                                                                                                                                     >  1:3638774/1‑150 (MQ=255)
   gTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATc                                                                                                                                                  <  1:3896850/150‑1 (MQ=255)
   gTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATc                                                                                                                                                  >  1:2614368/1‑150 (MQ=255)
    ttGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCa                                                                                                                                                 <  2:4043311/150‑1 (MQ=255)
       cGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTAGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGAGTCATATCCACCAGCGACAGCAACACTATGATCAAAt                                                                                                                                              >  2:568039/1‑150 (MQ=255)
        gTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATc                                                                                                                                             >  2:1309390/1‑150 (MQ=255)
         tCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCt                                                                                                                                            >  2:3463520/1‑150 (MQ=255)
          ccATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTg                                                                                                                                           <  1:2821896/150‑1 (MQ=255)
           cATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGa                                                                                                                                          >  2:1882950/1‑150 (MQ=255)
            aTTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGAt                                                                                                                                         >  2:3131403/1‑150 (MQ=255)
            aTTTTCCCAAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGAt                                                                                                                                         <  1:3654044/150‑1 (MQ=255)
              tttCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTc                                                                                                                                       <  2:1896294/150‑1 (MQ=255)
                  cccAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGcc                                                                                                                                   >  2:1604040/1‑150 (MQ=255)
                  cccAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATAGCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGcc                                                                                                                                   >  2:3026763/1‑150 (MQ=255)
                   ccagccagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCa                                                                                                                                  <  2:1742417/150‑1 (MQ=255)
                     agccagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATc                                                                                                                                >  1:1200991/1‑150 (MQ=255)
                      gccagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCg                                                                                                                               >  2:1747007/1‑150 (MQ=255)
                        cagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCgag                                                                                                                             <  2:2325643/150‑1 (MQ=255)
                        cagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCgag                                                                                                                             >  2:937927/1‑150 (MQ=255)
                           ttCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCACACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGagaag                                                                                                                          <  2:2254983/150‑1 (MQ=255)
                            tCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCAACGagaaga                                                                                                                         <  2:3845306/150‑1 (MQ=255)
                              aGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGata                                                                                                                       <  2:2676300/150‑1 (MQ=255)
                              aGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGata                                                                                                                       >  2:4153252/1‑150 (MQ=255)
                               gCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGatat                                                                                                                      >  1:2705087/1‑150 (MQ=255)
                                cTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATAtt                                                                                                                     >  1:3394250/1‑150 (MQ=255)
                                cTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATAtt                                                                                                                     <  1:3799522/150‑1 (MQ=255)
                                cTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATAtt                                                                                                                     >  2:357992/1‑150 (MQ=255)
                                  tctcTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCg                                                                                                                   <  1:2559169/150‑1 (MQ=255)
                                        tGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTa                                                                                                             <  2:101107/150‑1 (MQ=255)
                                         gTTCTCGTAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTAc                                                                                                            <  1:3939038/150‑1 (MQ=255)
                                         gTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCCCCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTAc                                                                                                            <  2:486963/150‑1 (MQ=255)
                                          ttCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACg                                                                                                           <  1:3749839/150‑1 (MQ=255)
                                            ctcGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTg                                                                                                         <  2:28156/150‑1 (MQ=255)
                                            ctcGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTg                                                                                                         <  2:28631/150‑1 (MQ=255)
                                               ggAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaat                                                                                                      >  1:2629632/1‑150 (MQ=255)
                                                    tatCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAAtct                                                                                                 <  2:790897/150‑1 (MQ=255)
                                                     atCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAAtctc                                                                                                >  1:294655/1‑150 (MQ=255)
                                                     atCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAAtctc                                                                                                >  1:2205634/1‑150 (MQ=255)
                                                      tCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTcc                                                                                               <  2:1096918/150‑1 (MQ=255)
                                                       ccAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCt                                                                                              <  2:3940586/150‑1 (MQ=255)
                                                         aGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTgg                                                                                            >  1:3305192/1‑150 (MQ=255)
                                                           cTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGgaa                                                                                          >  1:3995483/1‑150 (MQ=255)
                                                           cTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGgaa                                                                                          <  2:3915237/150‑1 (MQ=255)
                                                                agaCGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACt                                                                                     <  2:3769839/150‑1 (MQ=255)
                                                                    gAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAg                                                                                 <  1:395453/150‑1 (MQ=255)
                                                                     aaaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAgc                                                                                >  2:4273922/1‑150 (MQ=255)
                                                                          ctcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCCTCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAg                                                                           >  2:3523818/1‑150 (MQ=255)
                                                                          ctcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAg                                                                           <  1:2154130/150‑1 (MQ=255)
                                                                            cATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAggg                                                                         <  1:1664556/150‑1 (MQ=255)
                                                                              tAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCa                                                                       <  2:3303217/150‑1 (MQ=255)
                                                                              tAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCa                                                                       <  2:3259409/150‑1 (MQ=255)
                                                                                aCCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAAc                                                                     >  1:3520155/1‑150 (MQ=255)
                                                                                      aaaCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGc                                                               >  2:3833912/1‑150 (MQ=255)
                                                                                          atcaccatcaccAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGc                                                           >  2:3559522/1‑150 (MQ=255)
                                                                                           tcaccatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCg                                                          >  1:3507062/1‑150 (MQ=255)
                                                                                             accatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGaa                                                        >  2:4225779/1‑150 (MQ=255)
                                                                                                 tcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGc                                                    >  2:1338504/1‑150 (MQ=255)
                                                                                                  caccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGcc                                                   >  2:1406468/1‑150 (MQ=255)
                                                                                                   accaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCa                                                  >  2:4189861/1‑150 (MQ=255)
                                                                                                   accaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCa                                                  >  2:4189865/1‑150 (MQ=255)
                                                                                                     caGCAACCCGCCAACCAGCGTCATAACCCCCAGCGACAGCAACACGTGGAACAAATCTGATTCCGCAATCTAGAAGATATTCGGCATTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCaaa                                                <  1:1134386/150‑1 (MQ=255)
                                                                                                      aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAg                                               >  2:199255/1‑150 (MQ=255)
                                                                                                      aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAg                                               >  1:2101294/1‑150 (MQ=255)
                                                                                                         aaaCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTac                                            >  2:3016429/1‑150 (MQ=255)
                                                                                                             cGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGACAGGCCAAAGTACACgg                                        >  2:1152098/1‑150 (MQ=255)
                                                                                                             cGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACgg                                        <  2:3436807/150‑1 (MQ=255)
                                                                                                               ccAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACgggg                                      >  2:1206521/1‑150 (MQ=255)
                                                                                                               ccAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACgggg                                      <  1:702503/150‑1 (MQ=255)
                                                                                                                 aaCCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGc                                    <  1:557861/150‑1 (MQ=255)
                                                                                                                   ccAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCaa                                  <  2:2952128/150‑1 (MQ=255)
                                                                                                                      gCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAagca                               <  1:1908195/150‑1 (MQ=255)
                                                                                                                      gCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAagca                               >  2:280131/1‑150 (MQ=255)
                                                                                                                       cGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAagcag                              >  2:2138926/1‑150 (MQ=255)
                                                                                                                          cATATCCACCAGCGACAGCAACACGATGATCAAATCTGAGTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGcagcca                           <  1:2653467/150‑1 (MQ=255)
                                                                                                                          cATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGcagcca                           >  2:2726871/1‑150 (MQ=255)
                                                                                                                              tCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCga                       >  1:433796/1‑150 (MQ=255)
                                                                                                                               ccaccaGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCgag                      >  1:2124671/1‑150 (MQ=255)
                                                                                                                               ccaccaGCGACAGCAACACGAGGATCAAATCTGAGTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCgag                      <  2:61993/150‑1 (MQ=255)
                                                                                                                                caccaGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCgaga                     >  2:153354/1‑150 (MQ=255)
                                                                                                                                 accaGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGaa                    >  2:3117541/1‑150 (MQ=255)
                                                                                                                                  ccaGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAg                   <  2:3124688/150‑1 (MQ=255)
                                                                                                                                   caGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGc                  <  2:429766/150‑1 (MQ=255)
                                                                                                                                    aGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCa                 >  1:2068217/1‑150 (MQ=255)
                                                                                                                                     gCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCAt                <  1:3186361/150‑1 (MQ=255)
                                                                                                                                     gCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCAt                <  1:1882950/150‑1 (MQ=255)
                                                                                                                                      cGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATa               <  2:2408492/150‑1 (MQ=255)
                                                                                                                                      agACAGCAAAACGATTATCAAATCTGATTCCGCAATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATa               <  2:4023848/149‑1 (MQ=255)
                                                                                                                                        aCAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACa             >  1:138554/1‑150 (MQ=255)
                                                                                                                                         cAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACAt            <  1:172484/150‑1 (MQ=255)
                                                                                                                                          aGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACAtt           <  1:1011921/150‑1 (MQ=255)
                                                                                                                                           gCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTg          >  2:958091/1‑150 (MQ=255)
                                                                                                                                            cAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGc         >  2:3806267/1‑150 (MQ=255)
                                                                                                                                             aaCACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCa        >  1:4265971/1‑150 (MQ=255)
                                                                                                                                               cacGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCAtt      <  2:729721/150‑1 (MQ=255)
                                                                                                                                                acGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCAttt     >  1:508729/1‑150 (MQ=255)
                                                                                                                                                acGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCAttt     <  1:849178/150‑1 (MQ=255)
                                                                                                                                                acGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCAttt     <  1:2531612/150‑1 (MQ=255)
                                                                                                                                                 cGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCAtttt    >  1:1419427/1‑150 (MQ=255)
                                                                                                                                                 cGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCAtttt    >  2:3238473/1‑150 (MQ=255)
                                                                                                                                                   aGGATCAAATCTGAGTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCATTTTCa  <  2:2484397/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCATTTTCA  >  CP009273/3142520‑3142816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑