Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,365,607 G→T 91.6% I109I (ATC→ATA)  nanT ← sialic acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,365,6070GT91.6% 889.4 / 65.5 302I109I (ATC→ATA) nanTsialic acid transporter
Reads supporting (aligned to +/- strand):  ref base G (15/11);  new base T (160/116);  total (175/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

GACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCAGAGAT  >  CP009273/3365460‑3365753
                                                                                                                                                   |                                                                                                                                                  
 acgGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATg                                                                                                                                                 <  2:4252932/150‑1 (MQ=255)
  cgGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGt                                                                                                                                                >  1:1013814/1‑150 (MQ=255)
     cccccACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGc                                                                                                                                             >  1:3821714/1‑150 (MQ=255)
         cacaGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTgg                                                                                                                                         <  1:3221619/150‑1 (MQ=255)
          acaGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGgc                                                                                                                                        >  1:705714/1‑150 (MQ=255)
           caGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGgcg                                                                                                                                       <  2:2942743/150‑1 (MQ=255)
              agaAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCaa                                                                                                                                    >  1:3841409/1‑150 (MQ=255)
              agaAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCaa                                                                                                                                    >  1:146395/1‑150 (MQ=255)
                aaGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAg                                                                                                                                  <  2:1612206/150‑1 (MQ=255)
                   ccTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCg                                                                                                                               >  1:2924048/1‑150 (MQ=255)
                   ccTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCg                                                                                                                               >  2:2394152/1‑150 (MQ=255)
                    cTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGc                                                                                                                              <  1:1908765/150‑1 (MQ=255)
                       aaaTCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAgg                                                                                                                           <  1:2053524/150‑1 (MQ=255)
                          tCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCa                                                                                                                        <  2:2298180/150‑1 (MQ=255)
                          tCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCa                                                                                                                        <  2:2668874/150‑1 (MQ=255)
                          tCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCa                                                                                                                        <  1:1221085/150‑1 (MQ=255)
                           cAAAAAACCACTGGCTTTGTTACGCAGAAGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAg                                                                                                                       <  2:3798235/150‑1 (MQ=255)
                                 aCCACTGACTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTccc                                                                                                                 >  2:2841571/1‑150 (MQ=255)
                                  ccACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCg                                                                                                                <  1:4056376/150‑1 (MQ=255)
                                   cACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCgg                                                                                                               >  2:815898/1‑150 (MQ=255)
                                      tGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCg                                                                                                            >  2:2344023/1‑150 (MQ=255)
                                      tGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCg                                                                                                            >  1:920368/1‑150 (MQ=255)
                                      tGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCg                                                                                                            <  1:934673/150‑1 (MQ=255)
                                      tGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCg                                                                                                            <  2:1804463/150‑1 (MQ=255)
                                       ggCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCga                                                                                                           >  2:3721480/1‑150 (MQ=255)
                                        gCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgag                                                                                                          >  2:2587052/1‑150 (MQ=255)
                                          tttGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaa                                                                                                        >  1:3968211/1‑150 (MQ=255)
                                            tGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaaga                                                                                                      <  1:1350772/150‑1 (MQ=255)
                                                aCGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAAc                                                                                                  >  2:3401072/1‑150 (MQ=255)
                                                aCGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAAc                                                                                                  >  2:1952437/1‑150 (MQ=255)
                                                aCGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCACATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAAc                                                                                                  <  1:2771079/150‑1 (MQ=255)
                                                 cGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACg                                                                                                 >  1:2145649/1‑150 (MQ=255)
                                                  gCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGa                                                                                                >  2:4112377/1‑150 (MQ=255)
                                                   cAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGAt                                                                                               <  2:1491194/150‑1 (MQ=255)
                                                   cAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGAt                                                                                               <  1:984526/150‑1 (MQ=255)
                                                    aGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATg                                                                                              >  2:2076019/1‑150 (MQ=255)
                                                    aGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATg                                                                                              >  1:4145236/1‑150 (MQ=255)
                                                    aGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATg                                                                                              <  2:511665/150‑1 (MQ=255)
                                                     gATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGc                                                                                             >  1:1630676/1‑150 (MQ=255)
                                                      aTGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCt                                                                                            >  2:299748/1‑150 (MQ=255)
                                                       tGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTg                                                                                           >  2:448668/1‑150 (MQ=255)
                                                           atGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGa                                                                                       >  2:1821887/2‑150 (MQ=255)
                                                              gCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCa                                                                                    >  2:3158777/1‑150 (MQ=255)
                                                                cAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCAtt                                                                                  <  2:1544306/150‑1 (MQ=255)
                                                                    tttCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCa                                                                              <  2:334466/150‑1 (MQ=255)
                                                                    tttCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCa                                                                              <  2:213906/150‑1 (MQ=255)
                                                                    tttCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCa                                                                              <  2:3896233/150‑1 (MQ=255)
                                                                     ttCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAg                                                                             >  2:1559815/1‑150 (MQ=255)
                                                                          tGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAgacgac                                                                        <  1:3906009/150‑1 (MQ=255)
                                                                            aCATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGc                                                                      >  1:2557925/1‑150 (MQ=255)
                                                                              aTAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGccc                                                                    >  2:3211516/1‑150 (MQ=255)
                                                                              aTAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGccc                                                                    >  2:3490351/1‑150 (MQ=255)
                                                                               tAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCg                                                                   >  1:3641260/1‑150 (MQ=255)
                                                                                  gTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAg                                                                <  1:3468324/150‑1 (MQ=255)
                                                                                   tGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGc                                                               >  2:944650/1‑150 (MQ=255)
                                                                                    ggCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCg                                                              <  1:4074743/150‑1 (MQ=255)
                                                                                      cgcTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGt                                                            <  1:880589/150‑1 (MQ=255)
                                                                                          ggAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCAcc                                                        >  1:3179060/1‑150 (MQ=255)
                                                                                           gAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCAccc                                                       >  2:3637798/1‑150 (MQ=255)
                                                                                            aaCCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCa                                                      >  2:3466666/1‑150 (MQ=255)
                                                                                             aCCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCAt                                                     <  1:3275352/150‑1 (MQ=255)
                                                                                             aCCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCAt                                                     <  2:624571/150‑1 (MQ=255)
                                                                                                   ttCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGcc                                               <  2:2612759/150‑1 (MQ=255)
                                                                                                    tCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCg                                              <  1:3137110/150‑1 (MQ=255)
                                                                                                    tCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCg                                              >  1:2940693/1‑150 (MQ=255)
                                                                                                     cACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGa                                             <  1:1673817/150‑1 (MQ=255)
                                                                                                       cccGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGc                                           >  1:2368352/1‑150 (MQ=255)
                                                                                                         cGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCAt                                         >  1:250119/1‑150 (MQ=255)
                                                                                                         cGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCAt                                         >  1:2426039/1‑150 (MQ=255)
                                                                                                         cGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCAt                                         >  1:838173/1‑150 (MQ=255)
                                                                                                          gCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATc                                        <  2:3484677/150‑1 (MQ=255)
                                                                                                               ccccATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGgcc                                   <  1:2289824/150‑1 (MQ=255)
                                                                                                               ccccATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGgcc                                   <  2:3072483/150‑1 (MQ=255)
                                                                                                                   aTGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGccgccg                               >  2:4004757/1‑150 (MQ=255)
                                                                                                                    tGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGa                              <  1:2737698/150‑1 (MQ=255)
                                                                                                                        gATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGACCGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGAATCAGGCCGCCGAACCa                          >  2:1242204/1‑150 (MQ=255)
                                                                                                                          tGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGc                        >  2:1641551/1‑150 (MQ=255)
                                                                                                                           gACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCg                       <  1:1034949/150‑1 (MQ=255)
                                                                                                                             ccAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgag                     >  2:1715492/1‑150 (MQ=255)
                                                                                                                             ccAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgag                     <  1:2629140/150‑1 (MQ=255)
                                                                                                                               aGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgagag                   >  2:2473785/1‑150 (MQ=255)
                                                                                                                               aGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgagag                   >  2:3267477/1‑150 (MQ=255)
                                                                                                                               aGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgagag                   >  2:2628396/1‑150 (MQ=255)
                                                                                                                               aGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgagag                   >  1:1411967/1‑150 (MQ=255)
                                                                                                                                gACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgagaga                  >  1:1563342/1‑150 (MQ=255)
                                                                                                                                 aCGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGAt                 <  2:2364556/150‑1 (MQ=255)
                                                                                                                                 aCGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGAt                 <  1:787455/150‑1 (MQ=255)
                                                                                                                                  cGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATa                <  1:2033707/150‑1 (MQ=255)
                                                                                                                                    aGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATaaa              >  1:19209/1‑150 (MQ=255)
                                                                                                                                     gCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAg             <  2:4214164/150‑1 (MQ=255)
                                                                                                                                      cGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAgg            >  1:1601742/1‑150 (MQ=255)
                                                                                                                                       gATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGc           >  2:1701783/1‑150 (MQ=255)
                                                                                                                                       gATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACTCCCGTATCGGTCACCCATAGCGCCGAGCATCAGTCCGCCGAACCAGCGAGAGATAAAGGc           <  2:1103570/150‑1 (MQ=255)
                                                                                                                                       gATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGc           >  1:1171806/1‑150 (MQ=255)
                                                                                                                                         tAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTg         >  2:1290991/1‑150 (MQ=255)
                                                                                                                                          aaaCATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGc        >  2:2216169/1‑150 (MQ=255)
                                                                                                                                           aaCATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCa       <  1:3101453/150‑1 (MQ=255)
                                                                                                                                               tGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCAgaga   >  1:464610/1‑150 (MQ=255)
                                                                                                                                                ggTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCAGAGAt  <  1:3370787/150‑1 (MQ=255)
                                                                                                                                                ggTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCAGAGAt  <  1:1175077/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCAGAGAT  >  CP009273/3365460‑3365753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑