Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,473,139 G→T 100% I276I (ATC→ATA)  kefB ← potassium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,473,1390GT96.1% 668.1 / 13.0 204I276I (ATC→ATA) kefBpotassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (7/2);  new base T (103/92);  total (110/94)
Fisher's exact test for biased strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 210 total aligned reads displayed.

AACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAAC  >  CP009273/3472990‑3473287
                                                                                                                                                     |                                                                                                                                                    
      aTCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAg                                                                                                                                                <  1:1631405/150‑1 (MQ=255)
              tGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAAcc                                                                                                                                        >  1:2126996/1‑150 (MQ=255)
               gAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGTGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCg                                                                                                                                       <  1:1378415/150‑1 (MQ=255)
               gAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGACCAAACCg                                                                                                                                       >  1:384128/1‑150 (MQ=255)
                aGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAAc                                                                                                                                         <  2:1355081/147‑1 (MQ=255)
                aGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAAc                                                                                                                                         >  1:1355081/1‑147 (MQ=255)
                   tACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGagca                                                                                                                                   >  1:1249217/1‑150 (MQ=255)
                      gcacgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAgcagca                                                                                                                                <  2:373557/150‑1 (MQ=255)
                      gcacgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAgcagca                                                                                                                                >  2:2310943/1‑150 (MQ=255)
                        acgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAg                                                                                                                              >  1:2043417/1‑150 (MQ=255)
                            cATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCtt                                                                                                                          >  1:3993935/1‑150 (MQ=255)
                             aTACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCttt                                                                                                                         <  2:1173644/150‑1 (MQ=255)
                                 aaTCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAg                                                                                                                     >  1:522782/1‑150 (MQ=255)
                                 aaTCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAg                                                                                                                     >  2:3701514/1‑150 (MQ=255)
                                 aaTCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTGTGAAg                                                                                                                     >  2:1249542/1‑150 (MQ=255)
                                  aTCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAgg                                                                                                                    >  2:2914149/1‑150 (MQ=255)
                                     gcgcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGAt                                                                                                                 <  1:4205048/150‑1 (MQ=255)
                                      cgcCAGCAGATACAGCACGAGAATTTTCTCCGCCACGATCACAAACACGATTATCACTACCCAAAACAGATTGGTATAAAGCACCCAGATGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGTGCAGCAAGCCTTTGAAGTGATc                                                                                                                <  2:4205973/150‑1 (MQ=255)
                                       gcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCg                                                                                                               >  2:1774973/1‑150 (MQ=255)
                                         cagcagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGAt                                                                                                             >  2:2598812/1‑150 (MQ=255)
                                          agcagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATa                                                                                                            >  2:3156086/1‑150 (MQ=255)
                                              gATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCg                                                                                                        <  1:3046311/150‑1 (MQ=255)
                                                 aCAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGttt                                                                                                     <  2:309497/150‑1 (MQ=255)
                                                   aGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTcc                                                                                                   >  1:2335039/1‑150 (MQ=255)
                                                       cGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGtt                                                                                               <  2:2203951/150‑1 (MQ=255)
                                                         agaATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCa                                                                                             >  2:2957406/1‑150 (MQ=255)
                                                          gaATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCAt                                                                                            <  2:397652/150‑1 (MQ=255)
                                                           aaTTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATg                                                                                           <  1:661159/150‑1 (MQ=255)
                                                           aaTTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATg                                                                                           <  1:4018669/150‑1 (MQ=255)
                                                           aaTTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATg                                                                                           <  1:3141285/150‑1 (MQ=255)
                                                               ttCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGa                                                                                       >  1:1203516/1‑150 (MQ=255)
                                                                  aCCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGatat                                                                                    <  1:217514/150‑1 (MQ=255)
                                                                   ccGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATAtt                                                                                   >  1:4291511/1‑150 (MQ=255)
                                                                   ccGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATAtt                                                                                   <  1:3349300/150‑1 (MQ=255)
                                                                    cGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTc                                                                                  <  2:3758902/150‑1 (MQ=255)
                                                                    cGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTc                                                                                  >  1:592769/1‑150 (MQ=255)
                                                                    cGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTc                                                                                  <  2:3641198/150‑1 (MQ=255)
                                                                     gCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATCTCAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCa                                                                                 >  1:2260412/1‑150 (MQ=255)
                                                                     gCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCa                                                                                 >  2:3397785/1‑150 (MQ=255)
                                                                       caccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACt                                                                               >  2:1011635/1‑150 (MQ=255)
                                                                       caccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACt                                                                               <  2:3365328/150‑1 (MQ=255)
                                                                          caGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTc                                                                            >  1:3224050/1‑150 (MQ=255)
                                                                          caGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTc                                                                            >  1:1030706/1‑150 (MQ=255)
                                                                            gCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCg                                                                          <  2:1446233/150‑1 (MQ=255)
                                                                             cacaACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGc                                                                         >  1:85123/1‑150 (MQ=255)
                                                                                aaCCACGCTTATCACTTCCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCcag                                                                      >  1:384516/1‑150 (MQ=255)
                                                                                  ccACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCcagca                                                                    <  1:3113692/150‑1 (MQ=255)
                                                                                    aCGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCagcagc                                                                  >  2:1427048/1‑150 (MQ=255)
                                                                                    aCGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCagcagc                                                                  >  1:1168399/1‑150 (MQ=255)
                                                                                    aCGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCagcagc                                                                  >  2:4334598/1‑150 (MQ=255)
                                                                                     cGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCATTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAgcagca                                                                 <  2:3499980/150‑1 (MQ=255)
                                                                                        ttATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGc                                                              >  2:1701622/1‑150 (MQ=255)
                                                                                          aTCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGccc                                                            >  2:2734380/1‑150 (MQ=255)
                                                                                          aTCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGccc                                                            >  2:1353291/1‑150 (MQ=255)
                                                                                            cACTACCCACAACAGATGGGTATAACGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGc                                                          <  1:4333521/150‑1 (MQ=255)
                                                                                             aCTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCa                                                         >  1:3209455/1‑150 (MQ=255)
                                                                                              cTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCaa                                                        >  2:1514634/1‑150 (MQ=255)
                                                                                                 cccACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATaa                                                     >  1:500483/1‑150 (MQ=255)
                                                                                                  ccACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATaaa                                                    <  2:591781/150‑1 (MQ=255)
                                                                                                  ccACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATaaa                                                    >  2:4096761/1‑150 (MQ=255)
                                                                                                   caccacAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAACCCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAAt                                                   <  2:4185508/150‑1 (MQ=255)
                                                                                                   caccacAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCTATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAAt                                                   <  2:3266653/150‑1 (MQ=255)
                                                                                                      aacaGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTa                                                >  2:4312967/1‑150 (MQ=255)
                                                                                                      aacaGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTa                                                >  2:2796842/1‑150 (MQ=255)
                                                                                                        caGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTAcc                                              <  2:2043210/150‑1 (MQ=255)
                                                                                                        caGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTAcc                                              >  1:3512474/1‑150 (MQ=255)
                                                                                                         aGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCg                                             >  2:701145/1‑150 (MQ=255)
                                                                                                         aGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCg                                             >  2:3950120/1‑150 (MQ=255)
                                                                                                         aGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCg                                             >  2:1791223/1‑150 (MQ=255)
                                                                                                         aGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCg                                             >  2:1791000/1‑150 (MQ=255)
                                                                                                            tGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAgc                                          >  1:3868176/1‑150 (MQ=255)
                                                                                                             gggTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAgcg                                         <  2:641836/150‑1 (MQ=255)
                                                                                                             gggTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAgcg                                         <  1:986852/150‑1 (MQ=255)
                                                                                                             gggTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAgcg                                         <  1:4123519/150‑1 (MQ=255)
                                                                                                               gTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGcc                                       <  2:571933/150‑1 (MQ=255)
                                                                                                                 ataAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCAt                                     <  2:2134795/150‑1 (MQ=255)
                                                                                                                 ataAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCAt                                     >  1:2746137/1‑150 (MQ=255)
                                                                                                                  taAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATc                                    <  1:2539903/150‑1 (MQ=255)
                                                                                                                  taAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATc                                    <  1:3654285/150‑1 (MQ=255)
                                                                                                                   aaaGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCg                                   <  2:839974/150‑1 (MQ=255)
                                                                                                                    aaGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGa                                  >  1:1927938/1‑150 (MQ=255)
                                                                                                                      gCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACa                                >  1:4155426/1‑150 (MQ=255)
                                                                                                                      gCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACa                                <  2:522782/150‑1 (MQ=255)
                                                                                                                         ccccGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCTATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGcc                             >  2:1869339/1‑150 (MQ=255)
                                                                                                                            cGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCa                          >  1:3728587/1‑150 (MQ=255)
                                                                                                                            cGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCa                          >  1:2538825/1‑150 (MQ=255)
                                                                                                                             gAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAg                         >  1:1818481/1‑150 (MQ=255)
                                                                                                                             gAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAg                         >  2:1795415/1‑150 (MQ=255)
                                                                                                                                gTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgcgc                      >  1:468297/1‑150 (MQ=255)
                                                                                                                                  tGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCAt                    >  1:2600671/1‑150 (MQ=255)
                                                                                                                                   gAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATc                   >  2:2101784/1‑150 (MQ=255)
                                                                                                                                   gAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATc                   <  1:315886/150‑1 (MQ=255)
                                                                                                                                   gAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATAGACAGCCCCAGCGCATc                   >  1:1333536/1‑150 (MQ=255)
                                                                                                                                    aGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATcc                  <  1:789533/150‑1 (MQ=255)
                                                                                                                                     gTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCa                 <  1:3499015/150‑1 (MQ=255)
                                                                                                                                        aCATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATaa              <  2:1342070/150‑1 (MQ=255)
                                                                                                                                          aTGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAAc            >  1:3500773/1‑150 (MQ=255)
                                                                                                                                          aTGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAAc            >  1:1760074/1‑150 (MQ=255)
                                                                                                                                                  agatATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGa    <  1:627572/150‑1 (MQ=255)
                                                                                                                                                  agatATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGa    >  2:1605473/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAAC  >  CP009273/3472990‑3473287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑