Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,520,457 C→A 5.7% V210V (GTC→GTA)  yrfF → inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,520,4570CA 5.7% 1031.6 / 27.1 264V210V (GTC→GTA) yrfFinner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (126/123);  new base A (9/6);  total (135/129)
Fisher's exact test for biased strand distribution p-value = 5.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 276 total aligned reads displayed.

AGCTATGCCTTGCAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGAAATTCATTGTC  >  CP009273/3520308‑3520605
                                                                                                                                                     |                                                                                                                                                    
aGCTATGCCTTGCAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGAtcgtc                                                                                                                                                      >  2:2715480/1‑150 (MQ=255)
  cTATGCCTTGCAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGc                                                                                                                                                    >  2:3284497/1‑150 (MQ=255)
  cTATGCCTTGCAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGc                                                                                                                                                    >  2:1926206/1‑150 (MQ=255)
    aTGCCTTGCAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGcct                                                                                                                                                  <  1:386505/150‑1 (MQ=255)
     tGCCTTGCAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATTCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTctcctc                                                                                                                                                 >  1:3702354/1‑150 (MQ=255)
     tGCCTTGCAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGcctc                                                                                                                                                 <  2:3753320/150‑1 (MQ=255)
       ccTTGCAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGcctcct                                                                                                                                               <  2:3232383/150‑1 (MQ=255)
           gCAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCtccttcct                                                                                                                                           <  1:3143521/150‑1 (MQ=255)
            cAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTg                                                                                                                                          <  1:3181330/150‑1 (MQ=255)
             aaCCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGa                                                                                                                                         >  2:218381/1‑150 (MQ=255)
              aCCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGAt                                                                                                                                        <  1:3360170/150‑1 (MQ=255)
              aCCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCGGAt                                                                                                                                        >  2:145665/1‑150 (MQ=255)
               ccTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATg                                                                                                                                       >  1:2684258/1‑150 (MQ=255)
               ccTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATg                                                                                                                                       <  2:296801/150‑1 (MQ=255)
               ccTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATg                                                                                                                                       <  2:283516/150‑1 (MQ=255)
                  gTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTc                                                                                                                                    <  1:3782511/150‑1 (MQ=255)
                  gTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTAGCCTCCTTCCTGATGTTc                                                                                                                                    >  2:1481998/1‑150 (MQ=255)
                      cGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCtttt                                                                                                                                >  1:907403/1‑150 (MQ=255)
                       gTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCttttt                                                                                                                               <  1:732795/150‑1 (MQ=255)
                       gTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCttttt                                                                                                                               >  2:2727036/1‑150 (MQ=255)
                        tCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTc                                                                                                                              <  2:3836913/150‑1 (MQ=255)
                         cAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCt                                                                                                                             <  2:1640972/150‑1 (MQ=255)
                          aaCGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTg                                                                                                                            <  2:3881685/150‑1 (MQ=255)
                                  cGTCGATTCGCGGGGAAGAGAGTGAGCAACTCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTa                                                                                                                    <  2:2386269/150‑1 (MQ=255)
                                  cGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTa                                                                                                                    <  1:1341323/150‑1 (MQ=255)
                                   gTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTAc                                                                                                                   <  2:2002010/150‑1 (MQ=255)
                                      gATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTAcccc                                                                                                                >  1:629319/1‑150 (MQ=255)
                                      gATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTAcccc                                                                                                                <  1:2503777/150‑1 (MQ=255)
                                      gATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTAcccc                                                                                                                >  2:2957691/1‑150 (MQ=255)
                                       aTTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCg                                                                                                               <  2:1951023/150‑1 (MQ=255)
                                          cgcgGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGAt                                                                                                            >  1:398543/1‑150 (MQ=255)
                                            cgGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGt                                                                                                          <  1:211706/150‑1 (MQ=255)
                                            cgGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGt                                                                                                          >  2:3814120/1‑150 (MQ=255)
                                              gggAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTAt                                                                                                        >  2:3948363/1‑150 (MQ=255)
                                              gggAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTAt                                                                                                        <  1:4069391/150‑1 (MQ=255)
                                              gggAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTACGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTAt                                                                                                        <  2:1011691/150‑1 (MQ=255)
                                                gAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTAttt                                                                                                      <  1:3038509/150‑1 (MQ=255)
                                                gAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTAttt                                                                                                      <  1:4302433/150‑1 (MQ=255)
                                                 aaGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTg                                                                                                     >  1:2874074/1‑150 (MQ=255)
                                                 aaGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTg                                                                                                     <  1:142211/150‑1 (MQ=255)
                                                  agagagTGAGCAAATCGAGCTACTCAAGATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGt                                                                                                    <  2:4281972/150‑1 (MQ=255)
                                                   gagagTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGtt                                                                                                   <  1:2998343/150‑1 (MQ=255)
                                                    agagTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTc                                                                                                  >  2:1325107/1‑150 (MQ=255)
                                                      agTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCg                                                                                                <  1:1872220/150‑1 (MQ=255)
                                                          aGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTAGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGtggc                                                                                            >  1:1601233/1‑150 (MQ=255)
                                                           gCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGtggct                                                                                           <  1:1295912/150‑1 (MQ=255)
                                                            cAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGtggctg                                                                                          <  2:815853/150‑1 (MQ=255)
                                                             aaaTCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGtggctgg                                                                                         >  2:3126793/1‑150 (MQ=255)
                                                                 cGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAgg                                                                                     >  2:970470/1‑150 (MQ=255)
                                                                  gAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAggc                                                                                    >  1:4255452/1‑150 (MQ=255)
                                                                   aGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAggcg                                                                                   >  1:1774258/1‑150 (MQ=255)
                                                                     cTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAggcggc                                                                                 >  1:552714/1‑150 (MQ=255)
                                                                       aCTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGgcgc                                                                               >  1:3897523/1‑150 (MQ=255)
                                                                        cTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGcgcg                                                                              <  1:118167/150‑1 (MQ=255)
                                                                          cAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGtt                                                                            >  2:1026270/1‑150 (MQ=255)
                                                                           aaTATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTa                                                                           >  2:2782670/1‑150 (MQ=255)
                                                                              atTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTActg                                                                        >  1:686343/1‑150 (MQ=255)
                                                                              atTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTActg                                                                        <  2:3972262/150‑1 (MQ=255)
                                                                              atTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTActg                                                                        >  1:1282606/1‑150 (MQ=255)
                                                                              atTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTActg                                                                        >  1:1848695/1‑150 (MQ=255)
                                                                              atTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTActg                                                                        <  1:3811027/150‑1 (MQ=255)
                                                                               ttCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTActgc                                                                       <  1:2661796/150‑1 (MQ=255)
                                                                                 cGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTActgctg                                                                     <  2:2530362/150‑1 (MQ=255)
                                                                                   cAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTActgctgct                                                                   >  2:720069/1‑150 (MQ=255)
                                                                                    aaaGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTActgctgctg                                                                  >  1:2089773/1‑150 (MQ=255)
                                                                                     aaGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTgg                                                                 <  1:773650/150‑1 (MQ=255)
                                                                                     aaGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTAGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTgg                                                                 <  2:341036/150‑1 (MQ=255)
                                                                                       gAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGgc                                                               >  1:225309/1‑150 (MQ=255)
                                                                                       gAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGgc                                                               <  1:2966294/150‑1 (MQ=255)
                                                                                        aaaCGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGgcg                                                              <  1:3497218/150‑1 (MQ=255)
                                                                                             cATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGt                                                         >  1:1985113/1‑150 (MQ=255)
                                                                                               tGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTAGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCt                                                       >  1:738889/1‑150 (MQ=255)
                                                                                                 aagaaTATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCtgt                                                     >  1:1022538/1‑150 (MQ=255)
                                                                                                     agatGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTgggg                                                 <  1:2751587/148‑1 (MQ=255)
                                                                                                      tatGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTggggg                                                >  2:3692332/1‑150 (MQ=255)
                                                                                                      tatGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTggggg                                                >  1:1234761/1‑150 (MQ=255)
                                                                                                       atGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGc                                               >  2:4352790/1‑150 (MQ=255)
                                                                                                            cTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTc                                          <  2:3587500/150‑1 (MQ=255)
                                                                                                             tGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTcg                                         <  2:1774645/150‑1 (MQ=255)
                                                                                                               aGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTcgcg                                       >  2:1694373/1‑150 (MQ=255)
                                                                                                                gtcgtcCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCgcgc                                      >  1:3293269/1‑150 (MQ=255)
                                                                                                                   gtcCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGcccc                                   <  1:4143460/150‑1 (MQ=255)
                                                                                                                         gcgGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCaa                             <  1:2375208/150‑1 (MQ=255)
                                                                                                                          cgGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCaaa                            >  2:3036607/1‑150 (MQ=255)
                                                                                                                            ggCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAAt                          <  2:341919/150‑1 (MQ=255)
                                                                                                                                gCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAAtcctc                      <  2:4035972/150‑1 (MQ=255)
                                                                                                                                 cGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAAtcctcc                     <  1:457091/150‑1 (MQ=255)
                                                                                                                                  gTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTccc                    >  2:3765034/1‑150 (MQ=255)
                                                                                                                                   tGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCt                   <  1:2019577/150‑1 (MQ=255)
                                                                                                                                   tGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCt                   >  1:3850845/1‑150 (MQ=255)
                                                                                                                                    gAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTg                  <  2:2129183/150‑1 (MQ=255)
                                                                                                                                      aGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCg                >  1:3606116/1‑150 (MQ=255)
                                                                                                                                       gCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCgg               <  2:1225290/150‑1 (MQ=255)
                                                                                                                                        cGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCggg              >  2:3830796/1‑150 (MQ=255)
                                                                                                                                          ttGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGac            >  2:683518/1‑149 (MQ=255)
                                                                                                                                           tGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGaca           >  2:4210730/1‑148 (MQ=255)
                                                                                                                                              tGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGAAATTc        <  1:619211/150‑1 (MQ=255)
                                                                                                                                              tGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGAAATTc        >  1:3393706/1‑150 (MQ=255)
                                                                                                                                                 tcgtcgCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGAAATTCAtt     <  1:2483461/150‑1 (MQ=255)
                                                                                                                                                    tcgCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGAAATTCATTGTc  >  1:1775564/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGCTATGCCTTGCAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGAAATTCATTGTC  >  CP009273/3520308‑3520605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑