Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,822,339 A→C 10.2% Q12P (CAA→CCA) ‡ xanP → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,822,3390AC10.2% 404.3 / 5.2 199Q12P (CAA→CCA) ‡xanPxanthine permease
Reads supporting (aligned to +/- strand):  ref base A (103/69);  new base C (22/5);  total (125/74)
Fisher's exact test for biased strand distribution p-value = 3.32e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

TATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822190‑3822488
                                                                                                                                                     |                                                                                                                                                     
tatataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCa                                                                                                                                                       >  2:1701874/1‑150 (MQ=255)
 atataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTATTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCaa                                                                                                                                                      >  1:1581819/1‑150 (MQ=255)
  tataAAATAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAc                                                                                                                                                     <  1:1163529/150‑1 (MQ=255)
   ataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAcc                                                                                                                                                    >  2:3919200/1‑150 (MQ=255)
    taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCg                                                                                                                                                   <  2:3745684/150‑1 (MQ=255)
    taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCg                                                                                                                                                   <  1:1687460/150‑1 (MQ=255)
     aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCgg                                                                                                                                                  >  2:526390/1‑150 (MQ=255)
        aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTg                                                                                                                                               <  1:241890/150‑1 (MQ=255)
        aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTg                                                                                                                                               <  2:4043601/150‑1 (MQ=255)
          aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTgcg                                                                                                                                             >  2:4111328/1‑150 (MQ=255)
          aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgcg                                                                                                                                             >  1:1418650/1‑150 (MQ=255)
            tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCa                                                                                                                                           >  2:2355636/1‑150 (MQ=255)
             gCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCGGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAg                                                                                                                                          >  2:1179490/1‑150 (MQ=255)
               aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTTCGCAGAc                                                                                                                                        >  2:1092478/1‑150 (MQ=255)
               aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                                                                                                                        >  1:2462036/1‑150 (MQ=255)
               aTTTAGCTCATTTAAATATTTATATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                                                                                                                        <  2:2569509/150‑1 (MQ=255)
                  tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCa                                                                                                                                     >  1:3183628/1‑150 (MQ=255)
                   aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCaa                                                                                                                                    >  2:1284318/1‑150 (MQ=255)
                     ctcATTTAAATATTTAGATGTGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCaaaa                                                                                                                                  <  2:3219169/150‑1 (MQ=255)
                     ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCCCTTGGGCAGACACcaaa                                                                                                                                  >  1:223956/1‑150 (MQ=255)
                     ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCaaaa                                                                                                                                  >  2:2110368/1‑150 (MQ=255)
                     ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCaaaa                                                                                                                                  >  2:1825925/1‑150 (MQ=255)
                       cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAACCGTTGCGCAGACTCaaaaaa                                                                                                                                >  1:3241172/1‑148 (MQ=255)
                        aTTTAAATATTTAGATGAGAATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAg                                                                                                                               >  2:3143269/1‑150 (MQ=255)
                            aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGGTGCGCAGACTCAAAAAAACGaa                                                                                                                           >  1:3088393/1‑150 (MQ=255)
                            aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACaaaaaacccaa                                                                                                                           >  1:3088462/1‑143 (MQ=255)
                             aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAAAAGCGAAc                                                                                                                          >  2:239104/1‑150 (MQ=255)
                             aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACACGGGTGCGCAGACTCAAAACAGCGAAc                                                                                                                          >  2:786324/1‑150 (MQ=255)
                              atatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACATCGAACt                                                                                                                         >  2:3119327/1‑150 (MQ=255)
                               tatTTAGATGAGGATGTGGCAAACGTTTGCTTTTGTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTg                                                                                                                        >  2:2618249/1‑150 (MQ=255)
                                atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACACAAAACAGCGAACTGa                                                                                                                       >  1:3362374/1‑150 (MQ=255)
                                    aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAATACTGCGAAGGGATTTa                                                                                                                   >  2:4129421/1‑150 (MQ=255)
                                    aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGGCACCCGTGGCGCAGACACAAAACATCGAACAGATTTa                                                                                                                   >  1:1580257/1‑150 (MQ=255)
                                    aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTa                                                                                                                   >  2:1939871/1‑150 (MQ=255)
                                      ttgtggttgtgGCAAAGGTTTGCTTTTCTCTTTCGGTCTGATAAAATGCCCGTTTTGCTACCAGGGGTTTGTTTTCGTTGTCTGTTTCCACCCTCGAGACATAAAATCCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAcc                                                                                                                 <  1:4342868/143‑1 (MQ=255)
                                                 gCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAAAGCCCGCTTTGCTACCTCGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGACAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGAt                                                                                                      <  2:1629917/150‑1 (MQ=255)
                                                  cAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAATATc                                                                                                     >  2:2045153/1‑150 (MQ=255)
                                                   aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCg                                                                                                    >  2:841304/1‑150 (MQ=255)
                                                   aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCg                                                                                                    >  2:3832482/1‑150 (MQ=255)
                                                   aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAATCAAAACAGCGAACAGATTTACCGTCTTTAatatag                                                                                                    >  2:1569402/1‑148 (MQ=255)
                                                        tttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACCGTTGCGCAGACTCAAAACAGCGAACAGATTTACCGTCTTTAAGATCGTcccc                                                                                               >  1:190508/1‑148 (MQ=255)
                                                         ttGATTTTCTCGTTCGGCCATCTAAAATGCCCTCTTTGCAACCAGGGGTTTTTTTTCGATGTCTGTTTCCCCCCTCGAGTAATAAAATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccgcc                                                                                              <  1:648794/150‑1 (MQ=255)
                                                           gCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCcgccgc                                                                                            >  2:3943773/1‑150 (MQ=255)
                                                              tttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCCAACTGATTTAACGTCTTTAAGATAGTCCGCCCCtta                                                                                         >  1:1316079/1‑149 (MQ=255)
                                                               ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGGTGCGCAGACTCAAAACAGAGAACTGATTTAACGTCTTGAAGATAGTCCGCCGCATcc                                                                                        >  1:2811133/1‑150 (MQ=255)
                                                                tctcTTGCGGTCAGCTAAATTGCCCGCTTTCCTACCACGGGATTGTTTTCGATGTCGGTTTCCACCCTCGAGACAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCt                                                                                       <  2:3069925/150‑1 (MQ=255)
                                                                      tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAcc                                                                                 >  1:3631525/1‑150 (MQ=255)
                                                                        ggTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCCCCCCGGGGCGCAGTCTCAAAACAACGACCAGATTTACCGTCTTGTAGTTAGTCCGCCCCGGCCTCAAAacca                                                                               >  1:41011/1‑149 (MQ=255)
                                                                         gTCAGCTAAAAAGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAAcccgg                                                                              >  2:3310017/1‑148 (MQ=255)
                                                                            ataaaaaaTGCCCGCTTTGCAACCTCTTTTTTTTTTTTTTTTTCTTTTACCACCCTCGATACAAAAAATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                           <  1:1509982/146‑1 (MQ=255)
                                                                            agatAAAATGCCCGCTTTGCTACCAGGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                           <  1:4326082/147‑1 (MQ=255)
                                                                            aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                           >  2:2497954/1‑150 (MQ=255)
                                                                            aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCACAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                           <  1:4257245/150‑1 (MQ=255)
                                                                            aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCACAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                           <  1:2068859/150‑1 (MQ=255)
                                                                              cTAAAAGGCCCGCTGTGCTACCTCGGTTTTGTTTTCGTTGTGTGTTACCACCCTCGAGTCAGAAATGGCGCACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                         <  1:2995969/150‑1 (MQ=255)
                                                                              atAAAATGCCCGATTTGCTACCACGGGATTGTTTTCTTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                         <  2:3831995/149‑1 (MQ=255)
                                                                                    tGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTg                                                                   <  2:3569991/150‑1 (MQ=255)
                                                                                     gCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTTTTTCCACCCTCTAGACAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGt                                                                  <  2:759892/150‑1 (MQ=255)
                                                                                       ccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACACAAAACAGCCAACAGATTTACCCTCGTGAATATCGTCCGCCCCATCCACAAAACCAGTTTGCCCCCAGTCa                                                                >  1:4269011/1‑150 (MQ=255)
                                                                                        cGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAg                                                               <  2:3454429/150‑1 (MQ=255)
                                                                                          ttttGCTACCTGGGGTTTTTTTTCGTTTTGTGTTACCCCCCGCGAGCCAGAAAATGCGCCCGCGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCa                                                             <  1:3651950/149‑1 (MQ=255)
                                                                                             tGCTACCTCGGGTTTGTTTTGGATGTCTGTTACCACCCTCGAGACAGAAATTGCGAACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                          <  1:2484243/150‑1 (MQ=255)
                                                                                             tGCTACCAGGGGATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCATAAACTGCGCACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                          <  2:444338/150‑1 (MQ=255)
                                                                                                 aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATcagctg                                                      >  2:4255975/1‑150 (MQ=255)
                                                                                                  ccAGGGGATGTTTTTCGCTGTCTGTGTCCACCCGCGAGCAAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTgg                                                     <  2:367789/150‑1 (MQ=255)
                                                                                                  caaggggATTGTTTTCTATGTCTTTTTCCACCCTCGAGTCATAAAATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTgg                                                     <  2:1817752/146‑1 (MQ=255)
                                                                                                           ttttttCTTTGTCTGTTCCCCCCCCCTAGTCAGAATATGCGCCCCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                            <  1:493713/148‑1 (MQ=255)
                                                                                                             ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTTCCGCCTGTCAGCATCTGCTGGCGATGTTCGtt                                          >  2:2141841/1‑150 (MQ=255)
                                                                                                                tCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCg                                       <  2:3816036/150‑1 (MQ=255)
                                                                                                                   aTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGTTTTAACCTGTTGGAGATCGTCCGCCGCTTGCTCAAACCCTGTTTTGCGCCTGTCAGCCTCTGCTGGCGATGTTAGTGGGGGTg                                    >  1:2328232/1‑150 (MQ=255)
                                                                                                                    tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCAAAAAACCTGTTTTCCGCCTGTCAGCATCTGCTGGCGATGTTAGTTGCGGTGa                                   >  1:250158/1‑150 (MQ=255)
                                                                                                                    tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCTCAAAACCTGTTTTCCGCCAGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                   >  2:742399/1‑150 (MQ=255)
                                                                                                                    tGTCTGTTCCCACCCTCGAGCCAGAAAAGGCGCACCGGGTCGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                   <  1:2005268/150‑1 (MQ=255)
                                                                                                                      tCTGTTCCCACCCTCGAGTCATAAAAGGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATc                                 <  1:242404/150‑1 (MQ=255)
                                                                                                                       gtGTTTCCCCCCTCGATCAACATAGTGCGCACCCGGTGCCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCa                                <  1:4121353/149‑1 (MQ=255)
                                                                                                                        tGTTTCCACCCTCGAGTCAGAAAATGCCCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCCGCATCTGCTGGCGCTGTTCGTTGCGGTGATCAc                               >  2:2674839/1‑150 (MQ=255)
                                                                                                                        tGTTTCCACCCTCGAGTCAGAAAAGGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCAc                               <  2:1003627/150‑1 (MQ=255)
                                                                                                                        tGTTCCCACCCGCTAGTCATAAAATGCGCACCGGTTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCAc                               <  1:633872/150‑1 (MQ=255)
                                                                                                                           ttCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcc                            >  2:292423/1‑150 (MQ=255)
                                                                                                                           ttCCACCCTCGAGTCAGAAAATGCGAAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcc                            <  2:1439159/150‑1 (MQ=255)
                                                                                                                            tCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGCACTGATTTAACGTCGTTAAGATAGTCCGCCGCTTCCTCAAAACCTGTTTTGCGCCGGTCAGCATATGCTGGCGATGTTGGTTGCGGTGTTCACGccc                           >  1:251813/1‑149 (MQ=255)
                                                                                                                            accACCCTCGACTCATAAAAGGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCa                           <  1:47006/149‑1 (MQ=255)
                                                                                                                              cACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACAAAAAACAGCGAACAGATTTACCCTCTTGAAGATCGTCCGCCGCTTCCACAAACCCAGTTTTCCGCCTGTCAGCATCTGCTGGCGATGTTCGGTGCGGTGATAcagccagc                         >  2:2556223/1‑150 (MQ=255)
                                                                                                                              cACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACGGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAGCACGCCAgc                         >  2:3896436/1‑150 (MQ=255)
                                                                                                                                   tCGAGCAAGAAAAGGCGCCCCGGGGGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTAt                    <  1:3889427/150‑1 (MQ=255)
                                                                                                                                    cGAGTCAGAAAATGCGCAACCGGTTTCCCAGACTCAAAAAAGCGCACAGATTTAACGTCTTGAAGATAGTCCGCCGCTTCCTCAAACCCAGTTTTCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgcgcgaat                   >  1:3485611/1‑146 (MQ=255)
                                                                                                                                       ttaataaatTGAGCCACGGGTTCCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAAt                <  1:1334596/145‑1 (MQ=255)
                                                                                                                                        tCACAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATc               <  2:573066/150‑1 (MQ=255)
                                                                                                                                         cAGAACAGGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt              <  1:3953452/150‑1 (MQ=255)
                                                                                                                                         cAGAAAATGCGCACCCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt              <  2:2965617/150‑1 (MQ=255)
                                                                                                                                         cAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt              >  2:3239116/1‑150 (MQ=255)
                                                                                                                                          aGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCAGTCACCAACTGCTGGCGATGTTCGTTGCGGGGATCACGCCAGCGCTATTAATATg             >  2:631801/1‑150 (MQ=255)
                                                                                                                                          aGAAAATGCGCAACCCGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAATATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGAAGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCtt             >  2:2802733/1‑149 (MQ=255)
                                                                                                                                             aaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTCTTAATCTGCCa          >  2:2224003/1‑150 (MQ=255)
                                                                                                                                              aaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAg         >  1:4294357/1‑150 (MQ=255)
                                                                                                                                              aaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCGGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAg         <  2:2623038/150‑1 (MQ=255)
                                                                                                                                                tGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgc       >  2:4059243/1‑150 (MQ=255)
                                                                                                                                                  cgcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCCGCGCTATTAATCTGCCAGgcgc     >  2:1960348/1‑150 (MQ=255)
                                                                                                                                                    cAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTg   >  2:4200483/1‑150 (MQ=255)
                                                                                                                                                     aaCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg  >  1:3610055/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822190‑3822488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑