Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,822,340 A→C 12.0% Q12H (CAA→CAC) ‡ xanP → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,822,3400AC12.0% 379.2 / 3.2 198Q12H (CAA→CAC) ‡xanPxanthine permease
Reads supporting (aligned to +/- strand):  ref base A (114/53);  new base C (10/21);  total (124/74)
Fisher's exact test for biased strand distribution p-value = 2.23e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 202 total aligned reads displayed.

ATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGG  >  CP009273/3822191‑3822489
                                                                                                                                                     |                                                                                                                                                     
atataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTATTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCaa                                                                                                                                                       >  1:1581819/1‑150 (MQ=255)
 tataAAATAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAc                                                                                                                                                      <  1:1163529/150‑1 (MQ=255)
  ataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAcc                                                                                                                                                     >  2:3919200/1‑150 (MQ=255)
   taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCg                                                                                                                                                    <  1:1687460/150‑1 (MQ=255)
    aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAcccg                                                                                                                                                   >  2:373686/1‑148 (MQ=255)
    aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCgg                                                                                                                                                   >  2:526390/1‑150 (MQ=255)
      aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGtt                                                                                                                                                 >  2:1544276/1‑150 (MQ=255)
       aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTg                                                                                                                                                <  1:952138/150‑1 (MQ=255)
       aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTg                                                                                                                                                <  1:241890/150‑1 (MQ=255)
       aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTg                                                                                                                                                <  2:4043601/150‑1 (MQ=255)
         aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTgcg                                                                                                                                              >  2:4111328/1‑150 (MQ=255)
         aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgcg                                                                                                                                              >  1:1418650/1‑150 (MQ=255)
           tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCa                                                                                                                                            >  2:2355636/1‑150 (MQ=255)
              aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                                                                                                                         >  2:1092487/1‑150 (MQ=255)
              aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                                                                                                                         >  1:2462036/1‑150 (MQ=255)
              aTTTAGCTCATTTAAATATTTATATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                                                                                                                         <  2:2569509/150‑1 (MQ=255)
               tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCCTTGCGCACACt                                                                                                                                        >  1:47692/1‑150 (MQ=255)
                 tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCa                                                                                                                                      >  1:3183628/1‑150 (MQ=255)
                    ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCCCTTGGGCAGACACcaaa                                                                                                                                   >  1:223956/1‑150 (MQ=255)
                    ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCaaaa                                                                                                                                   >  2:2110368/1‑150 (MQ=255)
                    ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCaaaa                                                                                                                                   >  2:1825925/1‑150 (MQ=255)
                      cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAACCGTTGCGCAGACTCaaaaaa                                                                                                                                 >  1:3241172/1‑148 (MQ=255)
                       aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAg                                                                                                                                >  2:3776018/1‑150 (MQ=255)
                          tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCACACACAAAAAAGCGa                                                                                                                             >  1:542220/1‑150 (MQ=255)
                           aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGGTGCGCAGACTCAAAAAAACGaa                                                                                                                            >  1:3088393/1‑150 (MQ=255)
                           aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACaaaaaacccaa                                                                                                                            >  1:3088462/1‑143 (MQ=255)
                           aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACACAAAACACCGac                                                                                                                            >  1:4209344/1‑149 (MQ=255)
                            aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAAAAGCGAAc                                                                                                                           >  2:239104/1‑150 (MQ=255)
                            aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACACGGGTGCGCAGACTCAAAACAGCGAAc                                                                                                                           >  2:786324/1‑150 (MQ=255)
                             atatTTAGATGAGGATGTGGCACACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCCCGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCTCAGACTCAAAACAGCGAAca                                                                                                                          >  2:3119358/1‑149 (MQ=255)
                              tatTTAGATGAGGATGTGGCAAACGTTTGCTTTTGTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTg                                                                                                                         >  2:2618249/1‑150 (MQ=255)
                               atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACACAAAACAGCGAACTGa                                                                                                                        >  1:3362374/1‑150 (MQ=255)
                                   aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAATACTGCGAAGGGATTTa                                                                                                                    >  2:4129421/1‑150 (MQ=255)
                                   aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGGCACCCGTGGCGCAGACACAAAACATCGAACAGATTTa                                                                                                                    >  1:1580257/1‑150 (MQ=255)
                                   aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTa                                                                                                                    >  2:1939871/1‑150 (MQ=255)
                                            tgtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGa                                                                                                           <  2:2713106/150‑1 (MQ=255)
                                              tgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGATTCAGAAAATGCGCAAACGGGTGCGCAGACTCAAAACACCGAACTGATTTAACGACATGAAg                                                                                                         >  2:3403065/1‑150 (MQ=255)
                                              tgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAg                                                                                                         >  2:1208491/1‑150 (MQ=255)
                                                 cAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATc                                                                                                      >  2:2808483/1‑150 (MQ=255)
                                                 cAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAATATc                                                                                                      >  2:2045153/1‑150 (MQ=255)
                                                  aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCg                                                                                                     >  2:841304/1‑150 (MQ=255)
                                                  aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACGGATTTACCGTCTTGAAGATCg                                                                                                     >  2:294426/1‑150 (MQ=255)
                                                       tttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACCGTTGCGCAGACTCAAAACAGCGAACAGATTTACCGTCTTTAAGATCGTcccc                                                                                                >  1:190508/1‑148 (MQ=255)
                                                          gCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCcgccgc                                                                                             >  2:3943773/1‑150 (MQ=255)
                                                             tttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCCAACTGATTTAACGTCTTTAAGATAGTCCGCCCCtta                                                                                          >  1:1316079/1‑149 (MQ=255)
                                                              ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGGTGCGCAGACTCAAAACAGAGAACTGATTTAACGTCTTGAAGATAGTCCGCCGCATcc                                                                                         >  1:2811133/1‑150 (MQ=255)
                                                                     tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAcc                                                                                  >  1:3631525/1‑150 (MQ=255)
                                                                     ccGGTCAGCTAACCGGCCCGCTTTGCTACCCCGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAcc                                                                                  <  2:3636471/149‑1 (MQ=255)
                                                                       ggTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCCCCCCGGGGCGCAGTCTCAAAACAACGACCAGATTTACCGTCTTGTAGTTAGTCCGCCCCGGCCTCAAAacca                                                                                >  1:41011/1‑149 (MQ=255)
                                                                        ttCAGATAAAATGCCGTGTTTGCTACCTTGTTTTTTTTTTCTTTGTTTTTTACCACCCTCGATTAACAAAATGCGCACGGGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTg                                                                               <  1:63636/149‑1 (MQ=255)
                                                                           ataaaaaaTGCCCGCTTTGCAACCTCTTTTTTTTTTTTTTTTTCTTTTACCACCCTCGATACAAAAAATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                            <  1:1509982/146‑1 (MQ=255)
                                                                           aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGGCACCGGTGGCGCAGACTCAAAACACCGCACAGTTTTAACGGCATTAAGATCGTCCGCCGCGTCAGCAAACCCAGttt                                                                            >  1:433799/1‑150 (MQ=255)
                                                                           aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                            >  2:2497954/1‑150 (MQ=255)
                                                                           aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                            >  2:325144/1‑150 (MQ=255)
                                                                           aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCACAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                            <  1:2068859/150‑1 (MQ=255)
                                                                             cTAAAAGGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                          <  2:3832064/150‑1 (MQ=255)
                                                                             cTAAAAGGCCCGCTGTGCTACCTCGGTTTTGTTTTCGTTGTGTGTTACCACCCTCGAGTCAGAAATGGCGCACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                          <  1:2995969/150‑1 (MQ=255)
                                                                               aaaaTGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTCGGTTTCCACCCTCGAGTCATAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccg                                                                        <  2:3224340/150‑1 (MQ=255)
                                                                                    gCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTTTTTCCACCCTCTAGACAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGt                                                                   <  2:759892/150‑1 (MQ=255)
                                                                                      ccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCCGACACAAAAAACCGAACAGATTTACCGTCTTGAAGAACGTCCGCCGCGACCGCAAACCCCGTTTGCCGCCAGTCa                                                                 >  1:4268961/1‑150 (MQ=255)
                                                                                      ccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCGTTAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTTCCGCCTGTCa                                                                 >  2:3466392/1‑150 (MQ=255)
                                                                                       cGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTTATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCAGTTTGCCGCCTGTCAg                                                                >  2:3100380/1‑150 (MQ=255)
                                                                                         ttttGCTACCTGGGGTTTTTTTTCGTTTTGTGTTACCCCCCGCGAGCCAGAAAATGCGCCCGCGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCa                                                              <  1:3651950/149‑1 (MQ=255)
                                                                                            tGCTACCTCGGGTTTGTTTTGGATGTCTGTTACCACCCTCGAGACAGAAATTGCGAACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                           <  1:2484243/150‑1 (MQ=255)
                                                                                            tGCTACCAGGGGATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCATAAACTGCGCACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                           <  2:444338/150‑1 (MQ=255)
                                                                                                aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgctg                                                       >  2:3173625/1‑150 (MQ=255)
                                                                                                aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATcagctg                                                       >  2:4255975/1‑150 (MQ=255)
                                                                                                 caaggggATTGTTTTCTATGTCTTTTTCCACCCTCGAGTCATAAAATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTgg                                                      <  2:1817752/146‑1 (MQ=255)
                                                                                                            ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTTCCGCCTGTCAGCATCTGCTGGCGATGTTCGtt                                           >  2:2141841/1‑150 (MQ=255)
                                                                                                               tCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCg                                        <  2:1626636/150‑1 (MQ=255)
                                                                                                                 gATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAGCGAACTGATTAACCGTCTTGAAGATCGTACGCCGCTTCCTCAAACACAGTTTGCAGCCTGTCAGCATCTGCTGGCGATGTTCGTTGAGGt                                      >  2:3062945/1‑150 (MQ=255)
                                                                                                                  aTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGTTTTAACCTGTTGGAGATCGTCCGCCGCTTGCTCAAACCCTGTTTTGCGCCTGTCAGCCTCTGCTGGCGATGTTAGTGGGGGTg                                     >  1:2328232/1‑150 (MQ=255)
                                                                                                                   tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCAAAAAACCTGTTTTCCGCCTGTCAGCATCTGCTGGCGATGTTAGTTGCGGTGa                                    >  1:250158/1‑150 (MQ=255)
                                                                                                                   tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCTCAAAACCTGTTTTCCGCCAGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                    >  2:742399/1‑150 (MQ=255)
                                                                                                                   tGTCTGTTCCCACCCTCGAGCCAGAAAAGGCGCACCGGGTCGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                    <  1:2005268/150‑1 (MQ=255)
                                                                                                                     tCTGTTCCCACCCTCGAGTCATAAAAGGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATc                                  <  1:242404/150‑1 (MQ=255)
                                                                                                                      gtGTTTCCCCCCTCGATCAACATAGTGCGCACCCGGTGCCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCa                                 <  1:4121353/149‑1 (MQ=255)
                                                                                                                       tGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCAc                                <  2:1003642/150‑1 (MQ=255)
                                                                                                                       tGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGGCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCAc                                >  2:3831117/1‑150 (MQ=255)
                                                                                                                       tGTTTCCACCCTCGAGTCAGAAAATGCCCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCCGCATCTGCTGGCGCTGTTCGTTGCGGTGATCAc                                >  2:2674839/1‑150 (MQ=255)
                                                                                                                        gTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAAAACCGAACTGATTTAACGTCATGAATATCGTCCGCCGCTTCCTAAAACCCAGTTTTCCGCCAGTCAGCAACTGCTGGCGAGGTTCGTTGCGGTGATAACg                               >  1:4293956/1‑150 (MQ=255)
                                                                                                                        gTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTTAAGATCGTCCGCCCCTTCCACAAACCCAGTTTGCCCCCTGTCAGCAAAATATGGCGATGTTCGTTGCGGTGTTAACg                               >  1:4294504/1‑150 (MQ=255)
                                                                                                                           tCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGCACTGATTTAACGTCGTTAAGATAGTCCGCCGCTTCCTCAAAACCTGTTTTGCGCCGGTCAGCATATGCTGGCGATGTTGGTTGCGGTGTTCACGccc                            >  1:251813/1‑149 (MQ=255)
                                                                                                                            ccACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAATATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcccg                           >  2:1396643/1‑148 (MQ=255)
                                                                                                                             cACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACAAAAAACAGCGAACAGATTTACCCTCTTGAAGATCGTCCGCCGCTTCCACAAACCCAGTTTTCCGCCTGTCAGCATCTGCTGGCGATGTTCGGTGCGGTGATAcagccagc                          >  2:2556223/1‑150 (MQ=255)
                                                                                                                             cACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACGGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAGCACGCCAgc                          >  2:3896436/1‑150 (MQ=255)
                                                                                                                                ccgcgcgTCACAACAGGCGCACCCGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCt                       <  1:43726/144‑1 (MQ=255)
                                                                                                                                  tCGAGCAAGAAAAGGCGCCCCGGGGGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTAt                     <  1:3889427/150‑1 (MQ=255)
                                                                                                                                   cGAGTCAGAAAATGCGCAACCGGTTTCCCAGACTCAAAAAAGCGCACAGATTTAACGTCTTGAAGATAGTCCGCCGCTTCCTCAAACCCAGTTTTCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgcgcgaat                    >  1:3485611/1‑146 (MQ=255)
                                                                                                                                      ttaataaatTGAGCCACGGGTTCCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAAt                 <  1:1334596/145‑1 (MQ=255)
                                                                                                                                       tCACAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATc                <  2:573066/150‑1 (MQ=255)
                                                                                                                                        cAGAACAGGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt               <  1:3953452/150‑1 (MQ=255)
                                                                                                                                         aGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCAGTCACCAACTGCTGGCGATGTTCGTTGCGGGGATCACGCCAGCGCTATTAATATg              >  2:631801/1‑150 (MQ=255)
                                                                                                                                            aaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTCTTAATCTGCCa           >  2:2224003/1‑150 (MQ=255)
                                                                                                                                             aaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCGGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAg          <  2:2623038/150‑1 (MQ=255)
                                                                                                                                               tGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgc        >  2:4059243/1‑150 (MQ=255)
                                                                                                                                                 cgcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCCGCGCTATTAATCTGCCAGgcgc      >  2:1960348/1‑150 (MQ=255)
                                                                                                                                                   cAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTg    >  2:4200483/1‑150 (MQ=255)
                                                                                                                                                    aaCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg   >  1:3610055/1‑150 (MQ=255)
                                                                                                                                                     aCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGTATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTggg  >  2:1773564/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGG  >  CP009273/3822191‑3822489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑