Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,988,075 T→A 94.1% V65E (GTG→GAG)  yifL → putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,988,0750TA94.1% 946.0 / 35.8 270V65E (GTG→GAG) yifLputative lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (9/7);  new base A (110/144);  total (119/151)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 270 total aligned reads displayed.

CTGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCTGG  >  CP009273/3987930‑3988221
                                                                                                                                                 |                                                                                                                                                  
cTGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAAt                                                                                                                                                >  2:1279462/1‑150 (MQ=255)
 tGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAAtt                                                                                                                                               >  2:1974616/1‑150 (MQ=255)
     gggCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTa                                                                                                                                           >  1:335759/1‑150 (MQ=255)
      ggCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTaa                                                                                                                                          <  2:4175408/150‑1 (MQ=255)
       gCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTaaa                                                                                                                                         <  1:3914131/150‑1 (MQ=255)
        cTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTaaaa                                                                                                                                        <  1:761237/150‑1 (MQ=255)
         tGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAg                                                                                                                                       <  2:678959/150‑1 (MQ=255)
             gTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAg                                                                                                                                   <  2:928635/150‑1 (MQ=255)
              tCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGt                                                                                                                                  >  1:4350567/1‑150 (MQ=255)
              tCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGt                                                                                                                                  >  1:4350345/1‑150 (MQ=255)
               cTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGtt                                                                                                                                 <  1:1031509/150‑1 (MQ=255)
                    aGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGt                                                                                                                            <  1:635975/150‑1 (MQ=255)
                    aGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGt                                                                                                                            <  1:3942714/150‑1 (MQ=255)
                     ggTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTa                                                                                                                           <  2:1998081/150‑1 (MQ=255)
                       tCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTAcc                                                                                                                         >  2:610723/1‑150 (MQ=255)
                       tCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTAcc                                                                                                                         >  2:3504288/1‑150 (MQ=255)
                        ccGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTAccc                                                                                                                        <  2:1552674/150‑1 (MQ=255)
                          gCTCTATGTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCcgc                                                                                                                      <  2:2441686/150‑1 (MQ=255)
                           ctctATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCcgcg                                                                                                                     >  2:212040/1‑150 (MQ=255)
                             ctATTTCCCGCCTGCAGATAAAAACGCATCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTg                                                                                                                   <  2:1848942/150‑1 (MQ=255)
                               aTTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGAt                                                                                                                 >  2:3816882/1‑150 (MQ=255)
                                 ttCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTg                                                                                                               >  1:2607790/1‑150 (MQ=255)
                                 ttCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTg                                                                                                               >  2:1202826/1‑150 (MQ=255)
                                  tCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTgg                                                                                                              <  1:1487211/150‑1 (MQ=255)
                                      gCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTa                                                                                                          <  1:2652102/150‑1 (MQ=255)
                                      gCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTa                                                                                                          <  1:3088979/150‑1 (MQ=255)
                                        cTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTaaa                                                                                                        <  2:1712700/150‑1 (MQ=255)
                                            aGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAatgat                                                                                                    <  2:718824/150‑1 (MQ=255)
                                            aGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAatgat                                                                                                    >  1:3177701/1‑150 (MQ=255)
                                             gATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTACatgatg                                                                                                   >  2:1928815/1‑150 (MQ=255)
                                             gATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAatgatg                                                                                                   >  1:4150406/1‑150 (MQ=255)
                                              aTAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGc                                                                                                  >  1:53707/1‑150 (MQ=255)
                                               tAAAAACGCACCGCCGCCGACCAACCCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCa                                                                                                 <  2:3733907/150‑1 (MQ=255)
                                               tAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCa                                                                                                 >  2:2549023/1‑150 (MQ=255)
                                                aaaaaCGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAg                                                                                                >  1:367440/1‑150 (MQ=255)
                                                   aaCGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTtc                                                                                             >  1:3669278/1‑150 (MQ=255)
                                                    aCGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTtct                                                                                            <  2:691131/150‑1 (MQ=255)
                                                      gCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCg                                                                                          <  1:2838994/150‑1 (MQ=255)
                                                      gCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCg                                                                                          >  1:3577311/1‑150 (MQ=255)
                                                            ccgccgACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATg                                                                                    >  2:3888544/1‑150 (MQ=255)
                                                                cgACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATg                                                                                <  2:2206371/150‑1 (MQ=255)
                                                                cgACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATg                                                                                <  1:2508296/150‑1 (MQ=255)
                                                                cgACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATg                                                                                >  2:3031388/1‑150 (MQ=255)
                                                                      aaCCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTg                                                                          <  2:1146603/150‑1 (MQ=255)
                                                                       aCCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTgg                                                                         <  2:3329025/150‑1 (MQ=255)
                                                                          ggTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCaa                                                                      >  1:1527241/1‑150 (MQ=255)
                                                                          ggTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCaa                                                                      <  2:2319944/150‑1 (MQ=255)
                                                                              gagaCGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGAt                                                                  <  1:2930216/150‑1 (MQ=255)
                                                                               agaCGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGAtt                                                                 <  1:1964232/150‑1 (MQ=255)
                                                                               agaCGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGAtt                                                                 >  2:1360244/1‑150 (MQ=255)
                                                                                gaCGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGAttt                                                                >  1:650698/1‑150 (MQ=255)
                                                                                 acgcaaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGAtttt                                                               <  1:1208292/150‑1 (MQ=255)
                                                                                  cgccaccgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTa                                                              <  2:4287650/144‑1 (MQ=255)
                                                                                  cgcaaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTa                                                              >  1:624649/1‑150 (MQ=255)
                                                                                     aaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATgg                                                           <  1:962079/150‑1 (MQ=255)
                                                                                       acgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGgtc                                                         <  2:1032731/150‑1 (MQ=255)
                                                                                        cgcaaTCCACGGTGCCGGATAAAAACGATTGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGgtcg                                                        <  2:642448/150‑1 (MQ=255)
                                                                                        cgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGgtcg                                                        >  2:3392333/1‑150 (MQ=255)
                                                                                         gcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGgtcgt                                                       <  1:1700844/150‑1 (MQ=255)
                                                                                          caaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGgtcgtc                                                      <  2:2531485/150‑1 (MQ=255)
                                                                                          caaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGgtcgtc                                                      <  1:3181443/150‑1 (MQ=255)
                                                                                           aaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGtcgtcg                                                     >  2:1403187/1‑150 (MQ=255)
                                                                                           aaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGtcgtcg                                                     <  1:2196444/150‑1 (MQ=255)
                                                                                            aTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGa                                                    >  2:4117785/1‑150 (MQ=255)
                                                                                             tCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGAc                                                   <  2:765633/150‑1 (MQ=255)
                                                                                              ccACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGAcg                                                  <  1:183722/150‑1 (MQ=255)
                                                                                                aCGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGAcgcg                                                <  1:347704/150‑1 (MQ=255)
                                                                                                    tGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTaa                                            <  1:804172/150‑1 (MQ=255)
                                                                                                     gCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAAc                                           <  1:1119379/150‑1 (MQ=255)
                                                                                                     gCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAAc                                           <  1:1748931/150‑1 (MQ=255)
                                                                                                      ccGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACg                                          >  2:2856004/1‑150 (MQ=255)
                                                                                                        ggATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCa                                        >  1:1645378/1‑150 (MQ=255)
                                                                                                         gATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAg                                       <  2:3850656/150‑1 (MQ=255)
                                                                                                          aTAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGa                                      >  2:3615189/1‑150 (MQ=255)
                                                                                                          aTAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGa                                      <  2:3425295/150‑1 (MQ=255)
                                                                                                          aTAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGa                                      <  1:553144/150‑1 (MQ=255)
                                                                                                           tAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGaa                                     <  1:1902575/150‑1 (MQ=255)
                                                                                                            aaaaaCGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGact                                    >  2:3442601/1‑148 (MQ=255)
                                                                                                            aaaaaCGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAAt                                    <  2:732963/150‑1 (MQ=255)
                                                                                                              aaaCGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGt                                  >  1:2800659/1‑150 (MQ=255)
                                                                                                                 cGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCtt                               >  2:796619/1‑150 (MQ=255)
                                                                                                                   aTCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCtttt                             <  2:3136880/150‑1 (MQ=255)
                                                                                                                    tCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCttttt                            <  1:1558119/150‑1 (MQ=255)
                                                                                                                      gcgcCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCttttttt                          <  1:3320184/150‑1 (MQ=255)
                                                                                                                        gcCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCa                        <  1:345123/150‑1 (MQ=255)
                                                                                                                        gcCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCa                        <  2:470445/150‑1 (MQ=255)
                                                                                                                         ccACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCAc                       <  2:673633/150‑1 (MQ=255)
                                                                                                                          cACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCAcc                      >  1:180688/1‑150 (MQ=255)
                                                                                                                          cACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCAcc                      >  2:2637690/1‑150 (MQ=255)
                                                                                                                              ggCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAg                  >  2:2333207/1‑150 (MQ=255)
                                                                                                                                cGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCACCGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCt                >  2:2066314/1‑150 (MQ=255)
                                                                                                                                cGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCTACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCt                >  1:3816071/1‑150 (MQ=255)
                                                                                                                                 gATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTg               <  1:4303792/150‑1 (MQ=255)
                                                                                                                                     gTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATtc           <  1:2385965/150‑1 (MQ=255)
                                                                                                                                     gTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATtc           >  1:598673/1‑150 (MQ=255)
                                                                                                                                     gTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATtc           <  1:211248/150‑1 (MQ=255)
                                                                                                                                      tCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATtcg          >  1:2197061/1‑150 (MQ=255)
                                                                                                                                          tCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGc      >  2:2878626/1‑150 (MQ=255)
                                                                                                                                          tCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGc      >  1:1421249/1‑150 (MQ=255)
                                                                                                                                             cAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCTg   <  1:1398625/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCTGG  >  CP009273/3987930‑3988221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑