Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,066,133 T→G 93.9% S267R (AGC→CGC)  yihU ← gamma‑hydroxybutyrate dehydrogenase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,066,1330TG93.9% 1181.0 / 30.7 321S267R (AGC→CGC) yihUgamma‑hydroxybutyrate dehydrogenase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (15/6);  new base G (132/168);  total (147/174)
Fisher's exact test for biased strand distribution p-value = 2.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 323 total aligned reads displayed.

TGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGT  >  CP009273/4065987‑4066280
                                                                                                                                                  |                                                                                                                                                   
tGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCgata                                                                                                                                                  <  2:1691137/150‑1 (MQ=255)
  tCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATaaa                                                                                                                                                <  2:2418697/150‑1 (MQ=255)
   cGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAAc                                                                                                                                               <  1:2552791/150‑1 (MQ=255)
   cGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAAc                                                                                                                                               <  2:451193/150‑1 (MQ=255)
   cGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCAGCGCGCGCCTGGCGATAAAc                                                                                                                                               >  2:1436402/1‑150 (MQ=255)
     ttGATGGTGTACTGATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCt                                                                                                                                             <  2:3815306/150‑1 (MQ=255)
        aTGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACAATTTTAATTTTGCAGTCATCCCCGCCCCGACACCGACCTGTTACACAAATGCGGACCAGTACAGGCGACCCCGACCCCCCGCGCCCGCCAGGCGATAAACCAccc                                                                                                                                          >  2:764758/1‑150 (MQ=255)
        aTGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACAATTTTAATTTTGCAGTAAAACCCGCACAGAAAACGACCTGTTACAGAAATGCGGACCAGTACAGGCGAACCCCACCCCCCGCGCGCGCCAGGCGATaaaacaacc                                                                                                                                          >  1:2410353/1‑144 (MQ=255)
         tGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCg                                                                                                                                         <  2:628231/150‑1 (MQ=255)
         tGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCg                                                                                                                                         <  1:3785509/150‑1 (MQ=255)
           gtgtACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTg                                                                                                                                       <  2:4234180/150‑1 (MQ=255)
              tACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAgg                                                                                                                                    <  2:1900962/150‑1 (MQ=255)
              tACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAgg                                                                                                                                    <  1:815783/150‑1 (MQ=255)
              tACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAgg                                                                                                                                    <  2:2946548/150‑1 (MQ=255)
                  tATTCATTCATTTATTCCTTTATCCAGTCGTGACAAATTTAAATTTGCAGTCAAACCCGCACTTACACCGACCTGTTACAGAAAGGCGGACCAGTCCAGGCGACCCCGACCCCCAGCGCCCGCCAGGCGATAAACCACCCGAGAggcggc                                                                                                                                >  1:3268380/1‑150 (MQ=255)
                        ttcattTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAg                                                                                                                          <  2:3620643/150‑1 (MQ=255)
                         tcattTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGc                                                                                                                         <  2:3710531/150‑1 (MQ=255)
                         tcattTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGc                                                                                                                         <  2:649221/150‑1 (MQ=255)
                           attTATTCCTTTATCCAGTCGTTACAATTTTACTTTGGCAGTCATCCCGGCACTGACACGGAACTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCCGCgg                                                                                                                       >  2:3754534/1‑150 (MQ=255)
                              tATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCAt                                                                                                                    <  2:2018242/150‑1 (MQ=255)
                                ttCCTTTATCCAGTCGTTAAATTTTTAATTTTGGAGTGAAACCGGCCCAGACAACGACCAGGTCCAGAAATGCGGTCCACTCCAGGCGGCCGCGACCCCCGGCGCGCGCCAGGCGCTAAAACAACCCAGAGGCGGCCCCCCCCGGccatt                                                                                                                  >  2:2545710/1‑146 (MQ=255)
                                 tCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGTATGGCGGACCTGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTgg                                                                                                                 <  2:3266302/150‑1 (MQ=255)
                                  ccTGTATCCAGTCGTTACATTTTGACTGTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGc                                                                                                                <  2:3138125/150‑1 (MQ=255)
                                   cTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGca                                                                                                               <  1:3447375/150‑1 (MQ=255)
                                      tATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACAt                                                                                                            <  1:2757815/150‑1 (MQ=255)
                                         ccAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCa                                                                                                         <  2:2425027/150‑1 (MQ=255)
                                          cAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACAGACACGGACCTGTTCCAGAATTGCGGACCAGTCCTGGCGACCGCGACCCCCTGCGCGCGCCAGGCGATAAACCACCCCTGAGGCGGCCCCCACCCGCATTGGCACATGCAg                                                                                                        >  1:1311129/1‑150 (MQ=255)
                                           aGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCCTTGGCACATGCAGc                                                                                                       >  2:3584592/1‑150 (MQ=255)
                                           aGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGc                                                                                                       <  1:3874537/150‑1 (MQ=255)
                                           aGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGc                                                                                                       <  1:1686552/150‑1 (MQ=255)
                                           aGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGc                                                                                                       >  2:908271/1‑150 (MQ=255)
                                               gTTACCTTTTTACTGTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGt                                                                                                   <  2:1373492/150‑1 (MQ=255)
                                               gTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGt                                                                                                   >  1:2885069/1‑150 (MQ=255)
                                               gTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGt                                                                                                   <  1:2733938/150‑1 (MQ=255)
                                               gTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGt                                                                                                   >  1:1917010/1‑150 (MQ=255)
                                                 tACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTg                                                                                                 >  2:3402610/1‑150 (MQ=255)
                                                 tACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTg                                                                                                 <  1:2497298/150‑1 (MQ=255)
                                                 tACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTg                                                                                                 <  1:4172120/150‑1 (MQ=255)
                                                   cATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGc                                                                                               <  1:3200634/150‑1 (MQ=255)
                                                    aTTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCg                                                                                              <  1:444259/150‑1 (MQ=255)
                                                               ggCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCCGCGGCAGTGGCACATGCGGCTGGTTGGCGACATCAAGGGc                                                                                   >  1:1237751/1‑150 (MQ=255)
                                                                gCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCg                                                                                  <  1:1552001/150‑1 (MQ=255)
                                                                  aGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACAGGCAGCTGGTTGGCGACATCAAGGGCGAt                                                                                >  2:1273177/1‑150 (MQ=255)
                                                                   gTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATg                                                                               >  1:2305668/1‑150 (MQ=255)
                                                                     cATCCCTGCAATGAAACGGACCTGTTCCAGAATGGCGGACGAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCTTTGGCACATGCAGCTGGTTGTCGACATCAAGGGCGATGcc                                                                             <  2:4244602/150‑1 (MQ=255)
                                                                     cATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGcc                                                                             <  2:3036517/150‑1 (MQ=255)
                                                                     cATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGcc                                                                             >  1:3484478/1‑150 (MQ=255)
                                                                      aTCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCa                                                                            >  1:768381/1‑150 (MQ=255)
                                                                      aTCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCa                                                                            <  2:1485854/150‑1 (MQ=255)
                                                                      aTCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCAGTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGCTGCCa                                                                            >  2:22278/1‑150 (MQ=255)
                                                                        cccGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAg                                                                          >  1:2571689/1‑150 (MQ=255)
                                                                          cGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGAt                                                                        <  2:439951/150‑1 (MQ=255)
                                                                          cGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGAt                                                                        >  2:2500035/1‑150 (MQ=255)
                                                                           ggCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATc                                                                       <  1:1002833/150‑1 (MQ=255)
                                                                           ggCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATc                                                                       >  2:498407/1‑150 (MQ=255)
                                                                           ggCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATc                                                                       <  1:2003582/150‑1 (MQ=255)
                                                                             cACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCt                                                                     >  1:683090/1‑150 (MQ=255)
                                                                              aCTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCtt                                                                    >  2:2084205/1‑150 (MQ=255)
                                                                              aCTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCtt                                                                    <  1:1366894/150‑1 (MQ=255)
                                                                               cTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATcc                                                                      <  1:3627753/147‑1 (MQ=255)
                                                                               cTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCCAGATCCTTa                                                                   >  2:1940639/1‑150 (MQ=255)
                                                                                tGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTAt                                                                  >  2:1331697/1‑150 (MQ=255)
                                                                                tGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTAt                                                                  >  2:3276802/1‑150 (MQ=255)
                                                                                 gACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATg                                                                 <  2:3630499/150‑1 (MQ=255)
                                                                                   cacGGTCCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATggg                                                               <  2:2717259/150‑1 (MQ=255)
                                                                                   cacGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATggg                                                               <  2:1706036/150‑1 (MQ=255)
                                                                                       gACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAg                                                           <  1:2616804/150‑1 (MQ=255)
                                                                                        aCCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAga                                                          >  1:1433171/1‑150 (MQ=255)
                                                                                          cTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatc                                                        <  2:2996633/150‑1 (MQ=255)
                                                                                          cTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatc                                                        >  1:3123981/1‑150 (MQ=255)
                                                                                          cTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatc                                                        <  2:787088/150‑1 (MQ=255)
                                                                                               ccAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCa                                                   <  2:4142252/150‑1 (MQ=255)
                                                                                                 aGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATg                                                 >  1:4038837/1‑150 (MQ=255)
                                                                                                  gAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGa                                                <  1:3561105/150‑1 (MQ=255)
                                                                                                   aaTGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGaa                                               <  2:3536247/150‑1 (MQ=255)
                                                                                                    aTGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGTGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAg                                              >  1:4153769/1‑150 (MQ=255)
                                                                                                    aTGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAg                                              >  2:3966180/1‑150 (MQ=255)
                                                                                                      ggCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGc                                            <  1:2190380/150‑1 (MQ=255)
                                                                                                        cGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCgg                                          <  1:4060210/150‑1 (MQ=255)
                                                                                                         ggACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCggg                                         >  2:878376/1‑150 (MQ=255)
                                                                                                          gACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGga                                        <  2:3016658/150‑1 (MQ=255)
                                                                                                            ccAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGgaga                                      <  2:1878264/150‑1 (MQ=255)
                                                                                                            ccAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGgaga                                      <  1:3106796/150‑1 (MQ=255)
                                                                                                            ccAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGgaga                                      <  2:57432/150‑1 (MQ=255)
                                                                                                               gTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAg                                   >  2:682064/1‑150 (MQ=255)
                                                                                                               gTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAg                                   <  2:1669041/150‑1 (MQ=255)
                                                                                                                 ccTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAt                                 <  2:1319958/150‑1 (MQ=255)
                                                                                                                   tGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAtct                               >  1:84553/1‑150 (MQ=255)
                                                                                                                         ccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTg                         <  2:382948/150‑1 (MQ=255)
                                                                                                                         ccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTg                         >  1:2471594/1‑150 (MQ=255)
                                                                                                                          cgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGa                        <  1:241851/150‑1 (MQ=255)
                                                                                                                            cgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAgg                      >  1:3354328/1‑150 (MQ=255)
                                                                                                                              accCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCCAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGAc                    >  2:3166022/1‑150 (MQ=255)
                                                                                                                              accCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGAc                    >  1:544759/1‑150 (MQ=255)
                                                                                                                                         gcgcCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGcc         <  2:4013085/150‑1 (MQ=255)
                                                                                                                                            ccTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAgg      >  2:2736679/1‑150 (MQ=255)
                                                                                                                                            ccTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAgg      <  1:4332724/150‑1 (MQ=255)
                                                                                                                                              tGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGaa    <  1:1772439/150‑1 (MQ=255)
                                                                                                                                              tGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGaa    <  2:1111061/150‑1 (MQ=255)
                                                                                                                                              tGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGaa    <  2:38830/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGT  >  CP009273/4065987‑4066280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑