BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,097,822	G→T	93.1%	E115* (GAA→TAA)	pfkA →	6‑phosphofructokinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,097,822	0	G	T	93.1%	904.0 / 42.3	287	E115* (GAA→TAA)	pfkA	6‑phosphofructokinase I
Reads supporting (aligned to +/- strand): ref base G (10/10); new base T (111/155); total (122/165)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

GGTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGCG  >  CP009273/4097675‑4097967

ggTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTaa                                                                                                                                                 >  2:805487/1‑150 (MQ=255)

  tACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAAt                                                                                                                                               <  1:3907077/150‑1 (MQ=255)

  tACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAAt                                                                                                                                               <  1:572837/150‑1 (MQ=255)

  tACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAAt                                                                                                                                               >  1:1856033/1‑150 (MQ=255)

  tACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAAt                                                                                                                                               <  2:1504444/150‑1 (MQ=255)

    cGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATgg                                                                                                                                             >  1:65785/1‑150 (MQ=255)

               tCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGc                                                                                                                                  >  2:2212299/1‑150 (MQ=255)

                cTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCa                                                                                                                                 <  1:2312541/150‑1 (MQ=255)

                 tGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCAt                                                                                                                                <  1:1101092/150‑1 (MQ=255)

                  gcgcGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATc                                                                                                                               <  1:613246/150‑1 (MQ=255)

                    gcgTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCgg                                                                                                                             <  1:2796308/150‑1 (MQ=255)

                      gTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTc                                                                                                                           <  1:401461/150‑1 (MQ=255)

                          cccGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGcc                                                                                                                       <  1:4262130/150‑1 (MQ=255)

                           ccGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCg                                                                                                                      <  2:703906/150‑1 (MQ=255)

                            cGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCgg                                                                                                                     >  1:4177079/1‑150 (MQ=255)

                            cGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCgg                                                                                                                     >  1:3569161/1‑150 (MQ=255)

                                aTTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCAc                                                                                                                 <  2:652181/150‑1 (MQ=255)

                                 ttCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACt                                                                                                                <  1:1337052/150‑1 (MQ=255)

                                    cgcgACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATc                                                                                                             <  1:931776/150‑1 (MQ=255)

                                    cgcgACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATc                                                                                                             >  2:1471527/1‑150 (MQ=255)

                                    cgcgACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATc                                                                                                             <  2:918794/150‑1 (MQ=255)

                                      cgacgaGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGa                                                                                                           >  2:3705860/1‑150 (MQ=255)

                                       gacgaGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGAc                                                                                                          <  2:509814/150‑1 (MQ=255)

                                          gagaACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAAc                                                                                                       <  2:3686118/150‑1 (MQ=255)

                                           agaACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACg                                                                                                      <  2:3311775/150‑1 (MQ=255)

                                           agaACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACg                                                                                                      >  1:2104324/1‑150 (MQ=255)

                                             aaCATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTCTCGACAACGAc                                                                                                    >  2:3655667/1‑150 (MQ=255)

                                                  ccGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCaa                                                                                               <  1:67903/150‑1 (MQ=255)

                                                    gcgcCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAg                                                                                             <  2:3357387/150‑1 (MQ=255)

                                                     cgcCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAgg                                                                                            <  1:2437144/150‑1 (MQ=255)

                                                      gcCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGc                                                                                           >  2:58557/1‑150 (MQ=255)

                                                       ccGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCa                                                                                          <  1:3025681/150‑1 (MQ=255)

                                                         gTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACt                                                                                        >  1:979108/1‑150 (MQ=255)

                                                            gCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGAc                                                                                     >  1:125315/1‑150 (MQ=255)

                                                               aTCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTac                                                                                  >  2:1080032/1‑150 (MQ=255)

                                                               aTCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTac                                                                                  <  2:587628/150‑1 (MQ=255)

                                                                 cGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTacac                                                                                >  2:4227847/1‑150 (MQ=255)

                                                                  gAAAACCTGAAAAAACGTGGTATCGATGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACt                                                                               >  2:3497520/1‑150 (MQ=255)

                                                                  gAAAACCTGAAAAAACGTGGTATCGATGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACt                                                                               >  2:3071731/1‑150 (MQ=255)

                                                                   aaaaCCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTa                                                                              <  2:623136/150‑1 (MQ=255)

                                                                   aaaaCCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTa                                                                              <  1:2170503/150‑1 (MQ=255)

                                                                   aaaaCCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTa                                                                              >  2:1949169/1‑150 (MQ=255)

                                                                    aaaCCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTAt                                                                             >  2:2700450/1‑150 (MQ=255)

                                                                     aaCCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATc                                                                            >  1:1622129/1‑150 (MQ=255)

                                                                      aCCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCg                                                                           >  2:2602049/1‑150 (MQ=255)

                                                                         tGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGtt                                                                        <  1:3088758/150‑1 (MQ=255)

                                                                         tGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGtt                                                                        >  2:1632334/1‑150 (MQ=255)

                                                                          gAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGttt                                                                       <  1:2646365/150‑1 (MQ=255)

                                                                          gAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGttt                                                                       >  1:760674/1‑150 (MQ=255)

                                                                          gAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGttt                                                                       >  1:1126194/1‑150 (MQ=255)

                                                                             aaaaCGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTttctt                                                                    <  2:1611860/150‑1 (MQ=255)

                                                                                 cGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACt                                                                >  2:4017199/1‑150 (MQ=255)

                                                                                 cGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACt                                                                <  1:2679056/150‑1 (MQ=255)

                                                                                  gTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTg                                                               <  1:164196/150‑1 (MQ=255)

                                                                                    ggTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTgcg                                                             <  2:326637/150‑1 (MQ=255)

                                                                                      tATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCt                                                           >  2:823506/1‑150 (MQ=255)

                                                                                       aTCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTg                                                          <  1:38408/150‑1 (MQ=255)

                                                                                        tCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGa                                                         >  1:1826685/1‑150 (MQ=255)

                                                                                         cGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAg                                                        >  2:1798652/1‑150 (MQ=255)

                                                                                         cGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAg                                                        <  2:2106884/150‑1 (MQ=255)

                                                                                            cgcgCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCAc                                                     <  1:2278944/150‑1 (MQ=255)

                                                                                             gcgcTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCAcc                                                    <  2:1391433/150‑1 (MQ=255)

                                                                                                      gtTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGaa                                           <  2:2962223/150‑1 (MQ=255)

                                                                                                       ttATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAg                                          <  2:1165198/150‑1 (MQ=255)

                                                                                                        tATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGc                                         >  1:1478637/1‑150 (MQ=255)

                                                                                                         aTCGGCGGTGACTGTTAATACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACAATATCGGTTTCTTCACTGCGCTGAGAACAGTTGTAGAAGCg                                        >  2:4279393/1‑150 (MQ=255)

                                                                                                         aTCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCg                                        <  2:1867899/150‑1 (MQ=255)

                                                                                                           cggcggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGAt                                      >  2:3836003/1‑150 (MQ=255)

                                                                                                           cggcggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGAt                                      <  2:4177079/150‑1 (MQ=255)

                                                                                                           cggcggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGAt                                      <  2:2597366/150‑1 (MQ=255)

                                                                                                           cggcggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGAt                                      <  1:2328971/150‑1 (MQ=255)

                                                                                                           cggcggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGAt                                      >  2:1552372/1‑150 (MQ=255)

                                                                                                           cggcggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGAt                                      >  2:3136564/1‑150 (MQ=255)

                                                                                                           cggcggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGAt                                      >  2:1255991/1‑150 (MQ=255)

                                                                                                            ggcggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATc                                     <  2:2758597/150‑1 (MQ=255)

                                                                                                            ggcggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATc                                     <  2:461568/150‑1 (MQ=255)

                                                                                                            ggcggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATc                                     <  2:2290189/150‑1 (MQ=255)

                                                                                                             gcggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCg                                    <  1:1762809/150‑1 (MQ=255)

                                                                                                             gcggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCg                                    <  1:816799/150‑1 (MQ=255)

                                                                                                                gTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGCTCGAcc                                 <  1:3724953/150‑1 (MQ=255)

                                                                                                                    cGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCt                             <  1:3398570/150‑1 (MQ=255)

                                                                                                                      gTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGc                           >  1:3311961/1‑150 (MQ=255)

                                                                                                                      gTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGc                           >  1:3277373/1‑150 (MQ=255)

                                                                                                                           tACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGac                      <  2:3951158/150‑1 (MQ=255)

                                                                                                                            aCATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGaca                     >  2:2195171/1‑150 (MQ=255)

                                                                                                                             cATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGacac                    <  1:1500040/150‑1 (MQ=255)

                                                                                                                              aTGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACAcc                   <  1:2234252/150‑1 (MQ=255)

                                                                                                                                 ggTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACctct                <  2:2057537/150‑1 (MQ=255)

                                                                                                                                 ggTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACctct                <  2:357327/150‑1 (MQ=255)

                                                                                                                                 ggTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACctct                >  1:463866/1‑150 (MQ=255)

                                                                                                                                 ggTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACctct                >  1:2934174/1‑150 (MQ=255)

                                                                                                                                  gTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAACGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCtctt               <  1:3373181/150‑1 (MQ=255)

                                                                                                                                     cAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCtcttctt            >  1:3597128/1‑150 (MQ=255)

                                                                                                                                      aaTGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCtcttcttc           >  2:2537398/1‑150 (MQ=255)

                                                                                                                                      aaTGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCtcttcttc           >  2:2265963/1‑150 (MQ=255)

                                                                                                                                           gTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCAcc      <  2:4047481/150‑1 (MQ=255)

                                                                                                                                           gTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCAcc      <  1:3770211/150‑1 (MQ=255)

                                                                                                                                             cTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAg    <  2:3774774/150‑1 (MQ=255)

                                                                                                                                             cTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAg    <  1:1004026/150‑1 (MQ=255)

                                                                                                                                               gACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGCg  >  1:2503991/1‑150 (MQ=255)

GGTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGCG  >  CP009273/4097675‑4097967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`