BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,127,022	T→G	92.9%	E188D (GAA→GAC)	yijF ←	DUF1287 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,127,022	0	T	G	92.9%	1275.0 / 44.7	337	E188D (GAA→GAC)	yijF	DUF1287 family protein
Reads supporting (aligned to +/- strand): ref base T (16/10); new base G (182/129); total (198/139)
Fisher's exact test for biased strand distribution p-value = 8.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 345 total aligned reads displayed.

ATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCTT  >  CP009273/4126876‑4127168

attgtgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACAtcgtct                                                                                                                                                 <  1:719895/150‑1 (MQ=255)

attgtgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACAtcgtct                                                                                                                                                 <  2:741745/150‑1 (MQ=255)

attgtgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACAtcgtct                                                                                                                                                 <  1:2097214/150‑1 (MQ=255)

 ttgtgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATcgtctt                                                                                                                                                <  2:3991370/150‑1 (MQ=255)

      ctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTgc                                                                                                                                           >  1:369718/1‑150 (MQ=255)

       tgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTgcg                                                                                                                                          >  2:1894421/1‑150 (MQ=255)

       tgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTgcg                                                                                                                                          <  1:3824692/150‑1 (MQ=255)

        ggCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTgcgc                                                                                                                                         >  2:748638/1‑150 (MQ=255)

         gCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTgcgcg                                                                                                                                        <  1:3077785/150‑1 (MQ=255)

             tGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTg                                                                                                                                    <  2:2217698/150‑1 (MQ=255)

                 gcTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGcc                                                                                                                                >  1:2736129/1‑150 (MQ=255)

                  cTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCa                                                                                                                               <  2:4263350/150‑1 (MQ=255)

                       gAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGtt                                                                                                                          >  1:3244649/1‑150 (MQ=255)

                         gCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGACAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTAt                                                                                                                        <  1:867550/150‑1 (MQ=255)

                          ccGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCACTGTTATg                                                                                                                       >  2:3102559/1‑150 (MQ=255)

                             tAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTAt                                                                                                                    <  2:4066228/150‑1 (MQ=255)

                             tAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTAt                                                                                                                    <  2:1252937/150‑1 (MQ=255)

                              aaGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATc                                                                                                                   <  2:567256/150‑1 (MQ=255)

                                    aaaGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAgc                                                                                                             <  1:2954236/150‑1 (MQ=255)

                                       gaagCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAgcggc                                                                                                          >  2:1017804/1‑150 (MQ=255)

                                        aagCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAgcggcg                                                                                                         >  1:1315111/1‑150 (MQ=255)

                                         agCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGt                                                                                                        <  1:1748831/150‑1 (MQ=255)

                                            cGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTAcc                                                                                                     <  2:316884/150‑1 (MQ=255)

                                              tCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCATCGGCGTACCGt                                                                                                   >  1:3921816/1‑150 (MQ=255)

                                              tCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGt                                                                                                   >  2:3141136/1‑150 (MQ=255)

                                               cAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTc                                                                                                  <  1:1423107/150‑1 (MQ=255)

                                                 gtgtAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTcgc                                                                                                >  1:1459957/1‑150 (MQ=255)

                                                 gtgtAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTcgc                                                                                                >  2:3825959/1‑150 (MQ=255)

                                                     aaaaaGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGc                                                                                            >  2:3203082/1‑150 (MQ=255)

                                                     aaaaaGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGc                                                                                            >  2:1312435/1‑150 (MQ=255)

                                                     aaaaaGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGc                                                                                            >  2:2423140/1‑150 (MQ=255)

                                                     aaaaaGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGc                                                                                            >  2:254978/1‑150 (MQ=255)

                                                      aaaaGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCg                                                                                           <  2:2474608/150‑1 (MQ=255)

                                                      aaaaGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCg                                                                                           >  2:1215766/1‑150 (MQ=255)

                                                       aaaGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGa                                                                                          >  2:3017723/1‑150 (MQ=255)

                                                         aGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAg                                                                                        >  1:3457432/1‑150 (MQ=255)

                                                         aGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAg                                                                                        >  1:41777/1‑150 (MQ=255)

                                                           gATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGcc                                                                                      >  2:1174265/1‑150 (MQ=255)

                                                           gATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGcc                                                                                      <  2:2821836/150‑1 (MQ=255)

                                                           gATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGcc                                                                                      <  2:2864400/150‑1 (MQ=255)

                                                           gATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGcc                                                                                      >  2:449945/1‑150 (MQ=255)

                                                           gATCTGAATTTGTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTGCCTGCGCGCCTGCGCCAATGTTAGGTCTCCCCAGCGGCGTACCGTCGCGGGCGAAGcc                                                                                      <  2:3301393/150‑1 (MQ=255)

                                                               tGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCt                                                                                  >  1:3779132/1‑150 (MQ=255)

                                                                     ttttCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAacc                                                                            >  2:1604344/1‑150 (MQ=255)

                                                                     ttttCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAacc                                                                            >  2:1605678/1‑150 (MQ=255)

                                                                      tttCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTCTGTATCACAAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAacca                                                                           >  2:3744946/1‑150 (MQ=255)

                                                                        tcttcATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAaccacc                                                                         <  2:3463410/150‑1 (MQ=255)

                                                                        tcttcATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAaccacc                                                                         <  1:1180943/150‑1 (MQ=255)

                                                                          ttcATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCAcccc                                                                       >  2:1750937/1‑150 (MQ=255)

                                                                           tcATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCa                                                                      <  1:4253385/150‑1 (MQ=255)

                                                                            caagaggggCGATCACTTATTTAACAAAATAACGATAAAGCCCCACCAACCGCCAGTAAAACAGCACATAGTCATCCTTCGCGCCGGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAAACACCCCaa                                                                     >  2:1801060/6‑150 (MQ=255)

                                                                             aTGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAat                                                                    <  2:840102/150‑1 (MQ=255)

                                                                              tgtgGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAata                                                                   >  2:1426636/1‑150 (MQ=255)

                                                                               gtgGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAatat                                                                  <  1:4012703/150‑1 (MQ=255)

                                                                                 ggggCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATAtgt                                                                >  2:109323/1‑150 (MQ=255)

                                                                                  gggCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATAtgtg                                                               >  2:2299285/1‑150 (MQ=255)

                                                                                        tctcTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGcc                                                         >  2:3041601/1‑150 (MQ=255)

                                                                                         ctctTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGccc                                                        >  2:2580017/1‑150 (MQ=255)

                                                                                         ctctTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGccc                                                        <  2:3384425/150‑1 (MQ=255)

                                                                                         ctctTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGccc                                                        <  2:3773066/150‑1 (MQ=255)

                                                                                         ctctTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGccc                                                        <  2:69869/150‑1 (MQ=255)

                                                                                          tctTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCg                                                       <  1:2063263/150‑1 (MQ=255)

                                                                                             tATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTa                                                    >  2:2659965/1‑150 (MQ=255)

                                                                                              aTTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTAt                                                   >  1:4105672/1‑150 (MQ=255)

                                                                                              aTTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTAt                                                   <  1:111634/150‑1 (MQ=255)

                                                                                               tttAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATc                                                  <  2:2024706/150‑1 (MQ=255)

                                                                                                 tAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCa                                                >  1:2246772/1‑150 (MQ=255)

                                                                                                 tAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCa                                                <  1:1938349/150‑1 (MQ=255)

                                                                                                      aaaTAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGc                                           >  2:13638/1‑150 (MQ=255)

                                                                                                         tAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAg                                        >  1:3347961/1‑150 (MQ=255)

                                                                                                          aaCGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAgg                                       >  2:2788314/1‑150 (MQ=255)

                                                                                                            cGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGgag                                     <  2:2163759/150‑1 (MQ=255)

                                                                                                              aTAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGAc                                   >  1:1043545/1‑150 (MQ=255)

                                                                                                                aaTGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACaa                                 >  2:1165327/1‑150 (MQ=255)

                                                                                                                aaTGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACaa                                 >  1:1000765/1‑150 (MQ=255)

                                                                                                                aaTGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACaa                                 >  1:2837278/1‑150 (MQ=255)

                                                                                                                 aTGCCCCACCATCCGCCAGTGAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATTTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAat                                <  1:467687/150‑1 (MQ=255)

                                                                                                                    ccccACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATc                             <  1:1390390/150‑1 (MQ=255)

                                                                                                                    ccccACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCACAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATc                             <  2:2948466/150‑1 (MQ=255)

                                                                                                                     cccACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCg                            >  1:624633/1‑150 (MQ=255)

                                                                                                                            tCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCtt                     <  1:1675032/150‑1 (MQ=255)

                                                                                                                            tCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCtt                     >  1:3148609/1‑150 (MQ=255)

                                                                                                                            tCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCtt                     >  1:2473215/1‑150 (MQ=255)

                                                                                                                            tCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCtt                     <  1:1881387/150‑1 (MQ=255)

                                                                                                                             ccGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATCTGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTg                    <  1:2366125/150‑1 (MQ=255)

                                                                                                                              cGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGa                   >  2:2081274/1‑150 (MQ=255)

                                                                                                                                ccAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATa                 <  1:2976062/150‑1 (MQ=255)

                                                                                                                                  aGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGt               <  2:41777/150‑1 (MQ=255)

                                                                                                                                   gTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTc              >  2:3525353/1‑150 (MQ=255)

                                                                                                                                     tAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCAc            <  1:2166414/150‑1 (MQ=255)

                                                                                                                                     tAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCAc            <  1:2608469/150‑1 (MQ=255)

                                                                                                                                     tAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCAc            >  1:4149855/1‑150 (MQ=255)

                                                                                                                                          aGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTgggg       <  2:859319/150‑1 (MQ=255)

                                                                                                                                           gCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGt      <  2:778551/150‑1 (MQ=255)

                                                                                                                                            cacaTCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGtt     <  1:1080019/150‑1 (MQ=255)

                                                                                                                                            cacaTCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGtt     >  1:1361212/1‑150 (MQ=255)

                                                                                                                                            cacaTCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGtt     >  1:806124/1‑150 (MQ=255)

                                                                                                                                            cacaTCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTGGATAGTCACTGGGGtg     >  1:2634317/1‑149 (MQ=255)

                                                                                                                                            cacaTCGTCGTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGtt     <  1:4346846/150‑1 (MQ=255)

                                                                                                                                               aTCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCtt  <  1:155739/150‑1 (MQ=255)

ATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCTT  >  CP009273/4126876‑4127168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`