Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,211,803 G→A 100% P205S (CCT→TCT)  arpA ← ankyrin repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,211,8030GA95.6% 809.6 / 18.1 226P205S (CCT→TCT) arpAankyrin repeat protein
Reads supporting (aligned to +/- strand):  ref base G (7/3);  new base A (107/109);  total (114/112)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 226 total aligned reads displayed.

CTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGGACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGT  >  CP009273/4211657‑4211948
                                                                                                                                                  |                                                                                                                                                 
  ttATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGc                                                                                                                                              >  2:4133705/1‑150 (MQ=255)
        ttAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGaaa                                                                                                                                        >  1:3544620/1‑150 (MQ=255)
         tAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAAc                                                                                                                                       >  2:3816392/1‑150 (MQ=255)
          aGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAAcc                                                                                                                                      >  2:2733888/1‑150 (MQ=255)
           gCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCa                                                                                                                                     >  2:1477230/1‑150 (MQ=255)
            ccGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCaa                                                                                                                                    >  2:1320123/1‑150 (MQ=255)
             cGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAAc                                                                                                                                   <  2:2501595/150‑1 (MQ=255)
              ggACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACg                                                                                                                                  >  2:3204583/1‑150 (MQ=255)
              ggACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACg                                                                                                                                  >  1:1763286/1‑150 (MQ=255)
                aCCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGcc                                                                                                                                <  1:3684553/150‑1 (MQ=255)
                   gTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCaaa                                                                                                                             <  1:3665425/150‑1 (MQ=255)
                   gTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCaaa                                                                                                                             <  1:3428149/150‑1 (MQ=255)
                     cAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGt                                                                                                                           <  2:3677488/150‑1 (MQ=255)
                      aaaGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTc                                                                                                                          >  2:279904/1‑150 (MQ=255)
                      aaaGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTc                                                                                                                          >  1:517884/1‑150 (MQ=255)
                      aaaGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTc                                                                                                                          <  2:1014052/150‑1 (MQ=255)
                       aaGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCt                                                                                                                         <  1:243317/150‑1 (MQ=255)
                        aGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTg                                                                                                                        >  2:1230181/1‑150 (MQ=255)
                        aGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTg                                                                                                                        >  2:1151717/1‑150 (MQ=255)
                        aGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTg                                                                                                                        <  2:1739309/150‑1 (MQ=255)
                         gCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTgg                                                                                                                       <  2:3566696/150‑1 (MQ=255)
                          cATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGt                                                                                                                      <  1:2252794/150‑1 (MQ=255)
                          cATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGt                                                                                                                      <  2:3272235/150‑1 (MQ=255)
                              gTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGatttt                                                                                                                  >  1:3115417/1‑150 (MQ=255)
                               ttATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTg                                                                                                                 <  2:2359152/150‑1 (MQ=255)
                                    caccaGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCAttt                                                                                                            >  1:570799/1‑150 (MQ=255)
                                      ccaGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCAttttt                                                                                                          <  2:4079160/150‑1 (MQ=255)
                                        aGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTttg                                                                                                        >  1:2968146/1‑150 (MQ=255)
                                        aGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTttg                                                                                                        <  1:4162451/150‑1 (MQ=255)
                                         gTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTttgt                                                                                                       <  2:2577958/150‑1 (MQ=255)
                                         gTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTttgt                                                                                                       <  1:3594955/150‑1 (MQ=255)
                                            atatGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGa                                                                                                    <  2:742353/150‑1 (MQ=255)
                                               aggCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGt                                                                                                 >  1:102649/2‑150 (MQ=255)
                                                ggCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCCCCGCAGTCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTg                                                                                                <  1:2364146/150‑1 (MQ=255)
                                                ggCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTg                                                                                                >  1:2731118/1‑150 (MQ=255)
                                                     ccATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTga                                                                                           >  2:3883194/1‑150 (MQ=255)
                                                       aTAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTgaga                                                                                         >  2:3513234/1‑150 (MQ=255)
                                                         aaCACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGaaa                                                                                       >  1:2261482/1‑150 (MQ=255)
                                                             cTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAAtttt                                                                                   >  2:3003896/1‑150 (MQ=255)
                                                              tGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAAttttt                                                                                  >  1:624434/1‑150 (MQ=255)
                                                              tGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAAttttt                                                                                  >  2:601903/1‑150 (MQ=255)
                                                                 tGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAg                                                                               >  2:1760432/1‑150 (MQ=255)
                                                                   ccATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCt                                                                             >  1:3240976/1‑150 (MQ=255)
                                                                     aTTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGa                                                                           >  2:2106876/1‑150 (MQ=255)
                                                                     aTTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGa                                                                           >  2:2737154/1‑150 (MQ=255)
                                                                        tGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAaga                                                                        <  2:399603/150‑1 (MQ=255)
                                                                         gATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAagag                                                                       >  1:286249/1‑150 (MQ=255)
                                                                           ttCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTa                                                                     >  1:436891/1‑150 (MQ=255)
                                                                            tCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTaa                                                                    <  1:1375409/150‑1 (MQ=255)
                                                                               aTCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAatca                                                                 >  2:2779562/1‑150 (MQ=255)
                                                                               aTCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAatca                                                                 >  1:1610099/1‑150 (MQ=255)
                                                                                 cTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAatcatc                                                               >  2:1142820/1‑150 (MQ=255)
                                                                                 cTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAatcatc                                                               <  1:1538909/150‑1 (MQ=255)
                                                                                 cTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAatc                                                                  >  2:3465870/1‑147 (MQ=255)
                                                                                 cTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAatc                                                                  <  1:3465870/147‑1 (MQ=255)
                                                                                  ttGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCt                                                              <  2:2518008/150‑1 (MQ=255)
                                                                                  ttGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCt                                                              <  1:2005598/150‑1 (MQ=255)
                                                                                   tGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCtt                                                             <  1:2498217/150‑1 (MQ=255)
                                                                                      cAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGAt                                                          <  1:720576/150‑1 (MQ=255)
                                                                                      cAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGAt                                                          <  1:3131253/150‑1 (MQ=255)
                                                                                      cAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGAt                                                          <  1:4031974/150‑1 (MQ=255)
                                                                                         aaTATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGAgggg                                                       >  2:1175756/1‑150 (MQ=255)
                                                                                         aaTATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATggg                                                       >  2:1929693/1‑150 (MQ=255)
                                                                                            atGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGtt                                                    <  1:2725354/150‑1 (MQ=255)
                                                                                             tGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGttt                                                   <  1:1056107/150‑1 (MQ=255)
                                                                                               cTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGt                                                 <  2:501911/150‑1 (MQ=255)
                                                                                               cTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGt                                                 >  2:3915832/1‑150 (MQ=255)
                                                                                                  tCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCATAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCATCTGAAGAGTAATCATCTTGATGGGGTTTGTAAt                                              <  2:1808757/150‑1 (MQ=255)
                                                                                                  tCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAAt                                              >  1:2785076/1‑150 (MQ=255)
                                                                                                  tCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAAt                                              >  1:2785143/1‑150 (MQ=255)
                                                                                                     tAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCaa                                           >  1:1712400/1‑150 (MQ=255)
                                                                                                     tAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGGCGGGTTTTTGCCTTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGACGAGTAAACATCTTGATGGGGTTGGTACTCaa                                           >  1:442111/1‑150 (MQ=255)
                                                                                                       aCTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACa                                         <  2:981723/150‑1 (MQ=255)
                                                                                                       aCTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACa                                         <  2:1816198/150‑1 (MQ=255)
                                                                                                       aCTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACa                                         >  1:808274/1‑150 (MQ=255)
                                                                                                          aGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTc                                      <  1:2335321/150‑1 (MQ=255)
                                                                                                           gCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCt                                     <  1:4061406/150‑1 (MQ=255)
                                                                                                            cccGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTg                                    <  1:54640/150‑1 (MQ=255)
                                                                                                             ccGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGa                                   >  1:1722070/1‑150 (MQ=255)
                                                                                                                aaaTCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATaa                                <  1:398239/150‑1 (MQ=255)
                                                                                                                  aTCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAat                              <  2:4004065/150‑1 (MQ=255)
                                                                                                                    cACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTACTCATCTTGATGGGGTTTGTAATCAACATTCTGATACatat                            >  2:3526507/1‑150 (MQ=255)
                                                                                                                      ccGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCa                          >  2:4315162/1‑150 (MQ=255)
                                                                                                                        gcatgcagCAACGCATCGCCCCTCAGAACGTCTGGAAACCAACGCCATAGTCTGGTTTTTGCATTTTTTGTTGAATTGCGTGAGAAATTTTTCAGCTGATGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCAtt                        <  2:101344/150‑1 (MQ=255)
                                                                                                                           ggcagCAACGCAACGCCCCTCAGAANGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAat                     <  1:1109904/150‑1 (MQ=255)
                                                                                                                           ggcagCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAat                     >  1:1943352/1‑150 (MQ=255)
                                                                                                                            gcagcaACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAata                    <  2:4039586/150‑1 (MQ=255)
                                                                                                                               gcaacgcaacGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAatatat                 <  1:123920/150‑1 (MQ=255)
                                                                                                                                  acgcaacgcCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATtgt              <  1:428069/150‑1 (MQ=255)
                                                                                                                                    gcaacgcCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTgc            >  1:1512410/1‑150 (MQ=255)
                                                                                                                                     caacgcCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTgca           <  2:3653656/150‑1 (MQ=255)
                                                                                                                                      aacgcCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTgcag          >  2:1864453/1‑150 (MQ=255)
                                                                                                                                      aacgcCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTgcag          >  1:540891/1‑150 (MQ=255)
                                                                                                                                       acgcCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTgcagc         <  2:917695/150‑1 (MQ=255)
                                                                                                                                        cgcCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTgcagca        <  2:809298/150‑1 (MQ=255)
                                                                                                                                          ccccTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAAt      <  1:3816392/150‑1 (MQ=255)
                                                                                                                                          ccccTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAAt      <  2:3689252/150‑1 (MQ=255)
                                                                                                                                           cccTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAAtg     >  2:4083801/1‑150 (MQ=255)
                                                                                                                                            ccTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAAtgt    >  2:2855278/1‑150 (MQ=255)
                                                                                                                                             cTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAAtgtg   <  1:1990171/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGGACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGT  >  CP009273/4211657‑4211948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑