BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,373,797	T→C	15.7%	L48L (TTA→CTA)	yjeM →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,373,797	0	T	C	15.7%	652.6 / 102.3	242	L48L (TTA→CTA)	yjeM	putative transporter
Reads supporting (aligned to +/- strand): ref base T (99/105); new base C (21/17); total (120/122)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

ATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATC  >  CP009273/4373648‑4373946

aTGGTTTCATGCATCACACGTTAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCTTATTTGGATTTGCCAATAGACCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATCTTTTCTGCATTGt                                                                                                                                                       <  1:3254429/150‑1 (MQ=255)

  ggTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTa                                                                                                                                                     <  2:3392327/150‑1 (MQ=255)

   gTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTAt                                                                                                                                                    <  2:1701014/150‑1 (MQ=255)

   gTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTAt                                                                                                                                                    >  1:270257/1‑150 (MQ=255)

     ttCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATATCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGAATTGTTATTc                                                                                                                                                  >  1:1928911/1‑150 (MQ=255)

      tCATGCCTCACACGATAAAAAAGCTGAGTCTGATAGGAGTCCTATTGATGTTCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTCTAGTGCGGTTCCCGTGTATATATTTTCTGCATGGTTAttgg                                                                                                                                                 >  2:347697/1‑148 (MQ=255)

      tCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCt                                                                                                                                                 <  1:2318414/150‑1 (MQ=255)

       cATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCtt                                                                                                                                                >  2:3977915/1‑150 (MQ=255)

       cATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCtt                                                                                                                                                <  1:2812620/150‑1 (MQ=255)

        aTGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCttt                                                                                                                                               <  2:726140/150‑1 (MQ=255)

        aTGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGACAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCttt                                                                                                                                               >  2:3347700/1‑150 (MQ=255)

          gCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTAt                                                                                                                                             <  1:2498692/150‑1 (MQ=255)

             tCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCTATTCCCTTTTATATATTTTCTTCATTGTTATTCTTTATTcc                                                                                                                                          >  1:2607764/1‑150 (MQ=255)

               acacGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCAt                                                                                                                                        >  2:3542530/1‑150 (MQ=255)

               acacGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTACGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTGTATATTTTCTGCATTGTTATTCTTTATTCCAt                                                                                                                                        >  2:112658/1‑150 (MQ=255)

                  cGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCg                                                                                                                                     >  2:1909142/1‑150 (MQ=255)

                       aaaaaGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAAAGTGTTATAGTGCGATTCCCTTTTATATATTTTCTGCAGTGTTATTCTTTATTCCATTAGCCTTa                                                                                                                                >  1:869871/1‑150 (MQ=255)

                         aaaTATGAGTCTGATGGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGCTATTCTTTATTCCATTCGCCTTAat                                                                                                                              <  2:987254/150‑1 (MQ=255)

                         aaaGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGCTATTCTTTATTCCATTCGCCTTAat                                                                                                                              <  2:1536120/150‑1 (MQ=255)

                          aaGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAatg                                                                                                                             <  2:1000401/150‑1 (MQ=255)

                           aGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTc                                                                                                                                      <  1:1852012/140‑1 (MQ=255)

                               gAGTCGGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGc                                                                                                                        <  2:2596161/150‑1 (MQ=255)

                                   cTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGaa                                                                                                                    >  2:2235635/1‑150 (MQ=255)

                                        aGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATggg                                                                                                               <  1:2893220/150‑1 (MQ=255)

                                         ggACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGa                                                                                                              >  2:1369821/1‑150 (MQ=255)

                                          gACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAg                                                                                                             <  2:2411629/150‑1 (MQ=255)

                                           aCTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAgc                                                                                                            >  1:971699/1‑150 (MQ=255)

                                           aCTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAgc                                                                                                            >  2:3525297/1‑150 (MQ=255)

                                             tCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAgctg                                                                                                          <  1:3873816/150‑1 (MQ=255)

                                             tCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGCTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAgctg                                                                                                          >  1:3465981/1‑150 (MQ=255)

                                               atatTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAgctgct                                                                                                        <  2:2879953/150‑1 (MQ=255)

                                                 atTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTa                                                                                                      <  2:2123629/150‑1 (MQ=255)

                                                  ttGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGTGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGCTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTAt                                                                                                     >  2:3598860/1‑150 (MQ=255)

                                                   tgatgaTCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATc                                                                                                    >  1:518036/1‑150 (MQ=255)

                                                   tgatgaTCTTTACTTCCGTATTTGGATTTGCCAAAAGCCCAACGGCTTATTACTTAATGGGTTATAGTGCGATTACCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATc                                                                                                    >  2:3987075/1‑150 (MQ=255)

                                                    gatgatCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTTCTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCg                                                                                                   <  1:3599603/150‑1 (MQ=255)

                                                      tgatCTTTACGTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCa                                                                                                 <  2:2856807/150‑1 (MQ=255)

                                                          cTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAaaga                                                                                             >  1:549503/1‑150 (MQ=255)

                                                           tttACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAaagaa                                                                                            <  2:708564/150‑1 (MQ=255)

                                                            ttACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAaagaag                                                                                           <  1:4173054/150‑1 (MQ=255)

                                                            ttACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAaagaag                                                                                           <  1:2527062/150‑1 (MQ=255)

                                                               cTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAaagaagaag                                                                                        >  2:936904/1‑150 (MQ=255)

                                                               cTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAaagaagaag                                                                                        >  2:893150/1‑150 (MQ=255)

                                                                ttCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAgg                                                                                       >  1:3190347/1‑150 (MQ=255)

                                                                   cGTCTTTGGATTTGCCAAGAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTCTCTACTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGAGGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCgg                                                                                    <  2:3692428/150‑1 (MQ=255)

                                                                        ttGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCt                                                                               <  1:2859565/150‑1 (MQ=255)

                                                                        ttGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCt                                                                               <  1:1776528/150‑1 (MQ=255)

                                                                        ttGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGCTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCt                                                                               >  2:4321204/1‑150 (MQ=255)

                                                                         tGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGCTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTa                                                                              >  2:371106/1‑150 (MQ=255)

                                                                          ggATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTAt                                                                             <  2:3302469/150‑1 (MQ=255)

                                                                            aTTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTc                                                                           <  1:702458/150‑1 (MQ=255)

                                                                                   aaTAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATg                                                                    >  2:4260680/1‑150 (MQ=255)

                                                                                    aTAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGCTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGa                                                                   >  2:830537/1‑150 (MQ=255)

                                                                                     tAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGaa                                                                  <  2:1414827/150‑1 (MQ=255)

                                                                                      aGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGaat                                                                 <  1:1451498/150‑1 (MQ=255)

                                                                                         ccATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGaataat                                                              >  1:1084833/1‑150 (MQ=255)

                                                                                           aTCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAg                                                            >  1:1624465/1‑150 (MQ=255)

                                                                                           aTCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGCTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAg                                                            <  2:3614485/150‑1 (MQ=255)

                                                                                              ggCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAgtgt                                                         <  2:3427470/150‑1 (MQ=255)

                                                                                              ggCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAgtgt                                                         >  2:1978595/1‑150 (MQ=255)

                                                                                               gCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTc                                                        >  1:262964/1‑150 (MQ=255)

                                                                                                cTTATTACTTACTGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCg                                                       <  1:2005661/150‑1 (MQ=255)

                                                                                                 ttattaCTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCgg                                                      >  2:3564582/1‑150 (MQ=255)

                                                                                                  tattaCTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGa                                                     >  2:1348107/1‑150 (MQ=255)

                                                                                                  tattaCTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGa                                                     <  1:2235635/150‑1 (MQ=255)

                                                                                                     taCTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGCTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCa                                                  >  1:913748/1‑150 (MQ=255)

                                                                                                      aCTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCAc                                                 >  2:928280/1‑150 (MQ=255)

                                                                                                       cTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACg                                                <  2:1523755/150‑1 (MQ=255)

                                                                                                       cTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACg                                                >  2:1376414/1‑150 (MQ=255)

                                                                                                       cTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGCTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACg                                                >  2:2330956/1‑150 (MQ=255)

                                                                                                        ttAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTGTATTCCATTCGCCTGAATGATGGCTGAAATGGGAGCTGCTGATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGt                                               <  1:840362/150‑1 (MQ=255)

                                                                                                           aTGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGCTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGtttt                                            <  2:1490282/150‑1 (MQ=255)

                                                                                                            tgtgTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTg                                           <  2:3182516/147‑1 (MQ=255)

                                                                                                            tGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTg                                           >  1:2728142/1‑150 (MQ=255)

                                                                                                               gTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCt                                        >  1:64930/1‑150 (MQ=255)

                                                                                                                 tataGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTc                                      >  2:2024716/1‑150 (MQ=255)

                                                                                                                  ataGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCa                                     >  1:3939316/1‑150 (MQ=255)

                                                                                                                  ataGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCa                                     <  2:4196360/150‑1 (MQ=255)

                                                                                                                    aGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCAtt                                   <  1:1032550/150‑1 (MQ=255)

                                                                                                                    aGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCAtt                                   <  1:3128025/150‑1 (MQ=255)

                                                                                                                      tGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTgg                                 <  2:841726/150‑1 (MQ=255)

                                                                                                                         gATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTAc                              >  2:437306/1‑150 (MQ=255)

                                                                                                                          aTTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACg                             <  1:1155581/150‑1 (MQ=255)

                                                                                                                           ttCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATACGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGt                            >  2:2695624/1‑150 (MQ=255)

                                                                                                                            tCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGtt                           <  2:3639143/150‑1 (MQ=255)

                                                                                                                            tCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGtt                           >  2:3172976/1‑150 (MQ=255)

                                                                                                                            tCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGtt                           >  1:3366157/1‑150 (MQ=255)

                                                                                                                               cTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTAt                        >  2:1155619/1‑150 (MQ=255)

                                                                                                                               cTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTAt                        >  1:1001227/1‑150 (MQ=255)

                                                                                                                               cTTTTATATATTTTCTGCATTGCTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTAt                        <  1:3598860/150‑1 (MQ=255)

                                                                                                                                  ttATATATTTTCTGCATTGCTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTAtgtg                     >  1:1273806/1‑150 (MQ=255)

                                                                                                                                      atatTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGttt                 >  1:1718387/1‑150 (MQ=255)

                                                                                                                                       tatTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGtttt                >  1:955535/1‑150 (MQ=255)

                                                                                                                                        atTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTc               >  1:1391173/1‑150 (MQ=255)

                                                                                                                                            tCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCctct           <  1:3979169/150‑1 (MQ=255)

                                                                                                                                             cTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCtt          <  1:2816339/150‑1 (MQ=255)

                                                                                                                                             cTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCtt          <  2:1822510/150‑1 (MQ=255)

                                                                                                                                               gCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTtat        <  1:3542689/150‑1 (MQ=255)

                                                                                                                                                 aTTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTatat      >  1:4306109/1‑150 (MQ=255)

                                                                                                                                                     ttATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTGTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATatcatc  >  2:2044130/1‑150 (MQ=255)

ATGGTTTCATGCCTCACACGATAAAAAAGATGAGTCTGATAGGACTCATATTGATGATCTTTACTTCCGTATTTGGATTTGCCAATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATC  >  CP009273/4373648‑4373946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`