Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,396,598 A→T 100% E43V (GAA→GTA)  rnr → exoribonuclease R, RNase R

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,396,5980AT95.4% 1155.7 / 32.0 323E43V (GAA→GTA) rnrexoribonuclease R, RNase R
Reads supporting (aligned to +/- strand):  ref base A (11/4);  new base T (178/130);  total (189/134)
Fisher's exact test for biased strand distribution p-value = 2.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 324 total aligned reads displayed.

ATGACAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCC  >  CP009273/4396451‑4396744
                                                                                                                                                   |                                                                                                                                                  
aTGACAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTAc                                                                                                                                                  >  1:3669420/1‑150 (MQ=255)
aTGACAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTAc                                                                                                                                                  >  1:3419755/1‑150 (MQ=255)
  gACAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTg                                                                                                                                                >  1:771292/1‑150 (MQ=255)
   aCAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGc                                                                                                                                               >  2:2228005/1‑150 (MQ=255)
    cAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGca                                                                                                                                              <  1:553378/150‑1 (MQ=255)
     aaCGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGcac                                                                                                                                             >  1:4284082/1‑150 (MQ=255)
        ggaggaACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACAtt                                                                                                                                          <  1:355871/150‑1 (MQ=255)
            gaACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAg                                                                                                                                      <  2:2200240/150‑1 (MQ=255)
              aCCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGc                                                                                                                                    <  1:140936/150‑1 (MQ=255)
                cGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGa                                                                                                                                  <  2:3171789/150‑1 (MQ=255)
                        cacaAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGagcagc                                                                                                                          <  1:3348405/150‑1 (MQ=255)
                        cacaAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGagcagc                                                                                                                          >  2:277425/1‑150 (MQ=255)
                         acaAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCt                                                                                                                         <  1:1266748/150‑1 (MQ=255)
                         acaAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCt                                                                                                                         <  1:3641928/150‑1 (MQ=255)
                            aGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGa                                                                                                                      >  2:931340/1‑150 (MQ=255)
                            aGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGa                                                                                                                      >  1:621446/1‑150 (MQ=255)
                            aGATCATTTCAAGGAACGCGAATCTGAAAAATACGCGAATCCCATCCCTTGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTTATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGa                                                                                                                      <  1:3989991/150‑1 (MQ=255)
                             gATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGaa                                                                                                                     >  2:4324429/1‑150 (MQ=255)
                              aTCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAg                                                                                                                    >  2:2859464/1‑150 (MQ=255)
                               tCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAgg                                                                                                                   <  2:1392091/150‑1 (MQ=255)
                                ccTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGc                                                                                                                  >  1:3906911/1‑150 (MQ=255)
                                   ttCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTg                                                                                                               <  2:3190950/150‑1 (MQ=255)
                                       aGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTc                                                                                                           <  2:3964085/150‑1 (MQ=255)
                                        ggAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTcg                                                                                                          <  1:2793961/150‑1 (MQ=255)
                                        ggAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTcg                                                                                                          <  1:4014655/150‑1 (MQ=255)
                                         gAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACAGTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTcgc                                                                                                         >  2:1765285/1‑150 (MQ=255)
                                          aaCGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTcgcc                                                                                                        >  1:3375421/1‑150 (MQ=255)
                                              cgAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGACTTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCt                                                                                                    >  2:2063412/1‑150 (MQ=255)
                                              cgAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCt                                                                                                    <  1:3799371/150‑1 (MQ=255)
                                                aaGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTgc                                                                                                  >  2:4275496/1‑150 (MQ=255)
                                                  gCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTgcgc                                                                                                >  2:3022192/1‑150 (MQ=255)
                                                   cTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTgcgcg                                                                                               <  2:1268527/150‑1 (MQ=255)
                                                        aactACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCGTGAAGGCCTGCGTCGCCGCCTGCGCGCGATg                                                                                          >  2:1327687/4‑150 (MQ=255)
                                                        aaaTACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATg                                                                                          <  1:634027/150‑1 (MQ=255)
                                                         aaTACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATgg                                                                                         >  2:3455947/1‑150 (MQ=255)
                                                         aaTACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATgg                                                                                         >  1:407329/1‑150 (MQ=255)
                                                          aTACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGa                                                                                        >  2:1032372/1‑150 (MQ=255)
                                                           tACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAg                                                                                       >  2:2808695/1‑150 (MQ=255)
                                                           tACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAg                                                                                       >  2:4065244/1‑150 (MQ=255)
                                                           tACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAg                                                                                       >  2:1946903/1‑150 (MQ=255)
                                                            aCGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAgc                                                                                      <  2:2683179/150‑1 (MQ=255)
                                                             cgcgAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAgcg                                                                                     <  1:146752/150‑1 (MQ=255)
                                                                gAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGa                                                                                  >  2:757925/1‑150 (MQ=255)
                                                                gAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGa                                                                                  >  2:3511707/1‑150 (MQ=255)
                                                                  atcccatcccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATg                                                                                <  1:2228737/150‑1 (MQ=255)
                                                                   tcccatcccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATgg                                                                               <  1:226076/150‑1 (MQ=255)
                                                                   tcccatcccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATgg                                                                               <  1:1773700/150‑1 (MQ=255)
                                                                      catcccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCa                                                                            >  1:2496072/1‑150 (MQ=255)
                                                                       atcccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCaa                                                                           >  2:3598880/1‑150 (MQ=255)
                                                                         cccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACt                                                                         <  1:931340/150‑1 (MQ=255)
                                                                          ccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTg                                                                        <  2:2233395/150‑1 (MQ=255)
                                                                           cTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTgg                                                                       >  1:805328/1‑150 (MQ=255)
                                                                                tgggAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTc                                                                  >  1:3184161/2‑150 (MQ=255)
                                                                                cGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTc                                                                  <  2:1252864/150‑1 (MQ=255)
                                                                                 gggAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCa                                                                 >  1:3087657/1‑150 (MQ=255)
                                                                                  ggAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCAc                                                                >  2:659253/1‑150 (MQ=255)
                                                                                   gAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACt                                                               >  2:4049458/1‑150 (MQ=255)
                                                                                   gAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACt                                                               >  1:1076985/1‑150 (MQ=255)
                                                                                        tATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACtcgtcg                                                          >  1:1142688/1‑150 (MQ=255)
                                                                                         aTCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTAACTcgtcgt                                                         >  1:3033142/1‑150 (MQ=255)
                                                                                           ccTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCa                                                       <  2:604340/150‑1 (MQ=255)
                                                                                           ccTCGAACATATAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCa                                                       <  2:3759682/150‑1 (MQ=255)
                                                                                            cTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACAGGGAAGGCGCAGAGCAGCTTGTAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGGCAACGGGTCTTCCCTCGTCGTCAg                                                      >  2:2357891/1‑150 (MQ=255)
                                                                                             tCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGt                                                     <  2:1104875/150‑1 (MQ=255)
                                                                                               gAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGc                                                   >  1:2703503/1‑150 (MQ=255)
                                                                                               gAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGc                                                   >  1:530999/1‑150 (MQ=255)
                                                                                                 aCATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTa                                                 <  2:3718495/150‑1 (MQ=255)
                                                                                                  cATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTAt                                                >  2:2618049/1‑150 (MQ=255)
                                                                                                   aTTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATg                                               >  2:886260/1‑150 (MQ=255)
                                                                                                      tAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTAACTCGTCGTCAGTGCTATgcgc                                            >  1:446545/1‑150 (MQ=255)
                                                                                                       aaCCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCt                                           >  2:3077669/1‑150 (MQ=255)
                                                                                                       aaCCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCt                                           >  2:2121422/1‑150 (MQ=255)
                                                                                                               gTGAAACACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACCTTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAAc                                   <  2:1598167/150‑1 (MQ=255)
                                                                                                               gTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAAc                                   >  1:2424733/1‑150 (MQ=255)
                                                                                                                  aaaaaCCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGcc                                >  1:731719/1‑150 (MQ=255)
                                                                                                                   aaaaCCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCt                               >  1:3316442/1‑150 (MQ=255)
                                                                                                                     aaCCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCg                             >  2:2493184/1‑150 (MQ=255)
                                                                                                                      aCCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGa                            >  1:1337568/1‑150 (MQ=255)
                                                                                                                         ggCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCt                         >  2:4310433/1‑150 (MQ=255)
                                                                                                                         ggCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCt                         <  2:3203359/150‑1 (MQ=255)
                                                                                                                         ggCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCt                         <  2:1055191/150‑1 (MQ=255)
                                                                                                                           ccAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTgg                       <  2:1125310/150‑1 (MQ=255)
                                                                                                                            cAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTAACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGt                      <  2:1735240/150‑1 (MQ=255)
                                                                                                                             aGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTg                     >  1:3023068/1‑150 (MQ=255)
                                                                                                                                cGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGaaa                  >  1:2075981/1‑150 (MQ=255)
                                                                                                                                cGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGaaa                  >  1:4263559/1‑150 (MQ=255)
                                                                                                                                cGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTAACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGaaa                  >  2:4161149/1‑150 (MQ=255)
                                                                                                                                 gTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAg                 <  1:1032523/150‑1 (MQ=255)
                                                                                                                                 gTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAg                 <  2:3330853/150‑1 (MQ=255)
                                                                                                                                     tgaGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTAc             >  1:4205032/1‑150 (MQ=255)
                                                                                                                                      gaGCTGGCGGTCGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTAACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTAcc            >  1:2164819/1‑150 (MQ=255)
                                                                                                                                      gaGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGNACGCCTCGACCTGGTGAAAGGTAcc            >  1:1050209/1‑150 (MQ=255)
                                                                                                                                      gaGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTAca            <  2:2428442/150‑2 (MQ=255)
                                                                                                                                      gaGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGAGGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTAcc            <  2:531721/150‑1 (MQ=255)
                                                                                                                                       aGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCg           >  1:1971960/1‑150 (MQ=255)
                                                                                                                                       aGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCg           <  1:3511707/150‑1 (MQ=255)
                                                                                                                                          tGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTa        >  1:2327908/1‑150 (MQ=255)
                                                                                                                                             cGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTg     <  1:1482707/150‑1 (MQ=255)
                                                                                                                                               gtagtaCTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGc   <  1:675508/150‑1 (MQ=255)
                                                                                                                                                tAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTgggc  <  1:2592189/150‑3 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATGACAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGGCC  >  CP009273/4396451‑4396744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑