Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,402,850 G→T 42.5% L2L (CTG→CTT)  yjfC → ATP‑Grasp family ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,402,8500GT42.5% 209.8 / 316.6 254L2L (CTG→CTT) yjfCATP‑Grasp family ATPase
Reads supporting (aligned to +/- strand):  ref base G (77/69);  new base T (55/53);  total (132/122)
Fisher's exact test for biased strand distribution p-value = 8.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

Only 100 of 260 total aligned reads displayed.

CTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAACC  >  CP009273/4402703‑4402999
                                                                                                                                                   |                                                                                                                                                     
 tGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTATTACACCGCCCCGGGGCAGTGGGGTGGCGAATCATTCTtagt                                                                                                                                                    >  1:2936026/1‑149 (MQ=255)
  ggTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGac                                                                                                                                                   <  1:529048/150‑1 (MQ=255)
    tGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCAAGCAGAGaccc                                                                                                                                                 >  1:4297642/1‑148 (MQ=255)
          cTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGtt                                                                                                                                           <  1:1894260/150‑1 (MQ=255)
             cTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCt                                                                                                                                        <  1:2536802/150‑1 (MQ=255)
              tGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTTTGGTCGTTCTTCCAGCGCCCCTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCtg                                                                                                                                       <  1:4119400/150‑1 (MQ=255)
              tGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCtg                                                                                                                                       <  1:1366287/150‑1 (MQ=255)
               ggCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGGGGGCCGTGGGGAGGCTAATCATTCTGAGACACAACCttacagt                                                                                                                                      >  1:4151124/1‑145 (MQ=255)
                gcgcTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCtgtg                                                                                                                                     <  1:3522027/150‑1 (MQ=255)
                 cgcTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGc                                                                                                                                    >  2:671855/1‑150 (MQ=255)
                  gcTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCg                                                                                                                                   >  1:967161/1‑150 (MQ=255)
                    tCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGAc                                                                                                                                 <  2:3164545/150‑1 (MQ=255)
                       ggTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCCTTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACggg                                                                                                                              >  2:3904626/1‑150 (MQ=255)
                       ggTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACggg                                                                                                                              >  1:2787534/1‑150 (MQ=255)
                             ggCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTgg                                                                                                                        <  1:2279037/150‑1 (MQ=255)
                               cAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGAc                                                                                                                      <  1:1450093/150‑1 (MQ=255)
                               cAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGAc                                                                                                                      <  1:1450050/150‑1 (MQ=255)
                                 aaaaaCAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCa                                                                                                                    >  2:3396117/1‑150 (MQ=255)
                                     agagTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACCACGTTCCTGTGCGACGGGATCTGGACCAGATc                                                                                                                >  2:4175376/1‑150 (MQ=255)
                                     agagTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGATc                                                                                                                >  2:3541196/1‑150 (MQ=255)
                                     agagTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATc                                                                                                                >  2:466087/1‑150 (MQ=255)
                                     agagTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATc                                                                                                                <  2:3938076/150‑1 (MQ=255)
                                        gTCTGACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATcgcc                                                                                                             <  1:40546/150‑1 (MQ=255)
                                         tCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATATGGACCAGATcgccg                                                                                                            >  2:1133165/1‑150 (MQ=255)
                                          cTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTGTTCCAGCGCCCGTGGGCATTGGGGCGGCTAATCAAGCTGAGACACAACGTTCCTGTGCGACGGGATATGGACCAGATagccgc                                                                                                           >  1:2579869/1‑150 (MQ=255)
                                           ttACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCCTTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGATCGCCGCt                                                                                                          >  1:1557847/1‑150 (MQ=255)
                                           ttACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACCACGTTCCTGTGCTACGGGATCTGGACCAGATcgccgcg                                                                                                          >  2:1889311/1‑149 (MQ=255)
                                           ttACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCt                                                                                                          <  1:1241434/150‑1 (MQ=255)
                                            tACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCATCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATAGCCGCTg                                                                                                         >  1:2486658/1‑150 (MQ=255)
                                            tACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCCTTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTg                                                                                                         >  2:662729/1‑150 (MQ=255)
                                               tctTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTATTACAGCGCCCGGGGGGCTTTGGGGGGCGAATAATTCTTATACAAAAAGTTACATTTCGACCGGATCTGGAACAGATAGCCGCAGACa                                                                                                      >  1:3295424/1‑150 (MQ=255)
                                                ctTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACaa                                                                                                     >  2:3027663/1‑150 (MQ=255)
                                                 ttCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAAc                                                                                                    >  2:3530495/1‑150 (MQ=255)
                                                    gggCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGt                                                                                                 <  2:936666/150‑1 (MQ=255)
                                                    gggCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGt                                                                                                 >  2:3004335/1‑150 (MQ=255)
                                                    gggCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGt                                                                                                 <  1:3962774/150‑1 (MQ=255)
                                                      gcggcTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGATCGCCGCTGACAACGGttt                                                                                               <  2:2343319/150‑1 (MQ=255)
                                                      gcggcTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGttt                                                                                               <  1:2889550/150‑1 (MQ=255)
                                                       cggcTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTc                                                                                              <  1:3125151/150‑1 (MQ=255)
                                                       cggcTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTc                                                                                              <  1:2223290/150‑1 (MQ=255)
                                                       cggcTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCAGTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGCTCTGGACCAGATCGCCGCGGACAACGGTTTc                                                                                              >  2:3240021/1‑150 (MQ=255)
                                                          cTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGAc                                                                                           >  2:3973069/1‑150 (MQ=255)
                                                            ttACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACtt                                                                                         >  1:601244/1‑150 (MQ=255)
                                                            ttACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACtt                                                                                         >  2:1982221/1‑150 (MQ=255)
                                                             tACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACACCGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACttt                                                                                        <  1:3040502/150‑1 (MQ=255)
                                                              accaccAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGGGGGCCTTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTc                                                                                       >  1:2146586/1‑150 (MQ=255)
                                                                    aaaaaaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATatca                                                                                 >  2:2251060/1‑150 (MQ=255)
                                                                     aaaaaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATAATGCTGAGACACAACGTTACAGTGCGACCGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTatcatatcat                                                                                >  1:2716953/1‑150 (MQ=255)
                                                                       aaaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCg                                                                              >  1:276622/1‑150 (MQ=255)
                                                                           cGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACaa                                                                          <  2:1623753/150‑1 (MQ=255)
                                                                            gTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACCGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAAt                                                                         >  1:199944/1‑150 (MQ=255)
                                                                             tCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATg                                                                        <  1:1632913/150‑1 (MQ=255)
                                                                              cTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGa                                                                       <  2:2414234/150‑1 (MQ=255)
                                                                               tACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa                                                                      >  2:873536/1‑150 (MQ=255)
                                                                                 ccGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAAt                                                                    <  1:3613071/150‑1 (MQ=255)
                                                                                      tctcGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTAtt                                                               <  1:1725529/150‑1 (MQ=255)
                                                                                        tcGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTgg                                                             <  2:2855078/150‑1 (MQ=255)
                                                                                         cgcgGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTggg                                                            <  1:2655976/150‑1 (MQ=255)
                                                                                           cggcggCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGAt                                                          <  2:3740197/150‑1 (MQ=255)
                                                                                            ggcggcTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATg                                                         >  2:1371423/1‑150 (MQ=255)
                                                                                            ggcggcTATGGTCGTTCTGCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATg                                                         <  1:744180/150‑1 (MQ=255)
                                                                                                 cTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGt                                                    <  2:3381155/150‑1 (MQ=255)
                                                                                                 cTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGt                                                    <  1:923850/150‑1 (MQ=255)
                                                                                                       tCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCt                                              <  1:3525791/150‑1 (MQ=255)
                                                                                                       tCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCt                                              <  1:1268399/150‑1 (MQ=255)
                                                                                                         gTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTa                                            <  1:488233/150‑1 (MQ=255)
                                                                                                           tcttcCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTAcc                                          >  2:2813935/1‑150 (MQ=255)
                                                                                                           tcttcCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTAcc                                          >  2:253998/1‑150 (MQ=255)
                                                                                                           tcttcCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTAcc                                          <  1:1134702/150‑1 (MQ=255)
                                                                                                           tcttcCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTAcc                                          >  1:3841059/1‑150 (MQ=255)
                                                                                                            cttcCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCg                                         <  1:274357/150‑1 (MQ=255)
                                                                                                               ccAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGttt                                      >  2:2873020/1‑150 (MQ=255)
                                                                                                                 aGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTa                                    >  1:1769464/1‑150 (MQ=255)
                                                                                                                 aGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTa                                    >  2:494989/1‑150 (MQ=255)
                                                                                                                  gcgcCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTAc                                   <  2:1045759/150‑1 (MQ=255)
                                                                                                                    gcCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTActc                                 <  2:3947175/150‑1 (MQ=255)
                                                                                                                     cccGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTActct                                >  1:3674308/1‑150 (MQ=255)
                                                                                                                        gTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTgcg                             >  2:3616135/1‑150 (MQ=255)
                                                                                                                            gCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAg                         >  2:1357390/1‑150 (MQ=255)
                                                                                                                            gCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTAATCTGCGCCAg                         >  2:3337269/1‑150 (MQ=255)
                                                                                                                             cATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGTGAGTCGGGCTTACCGTTTTACTCTGCGCCAGa                        <  1:668065/150‑1 (MQ=255)
                                                                                                                             cATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGa                        <  1:3027663/150‑1 (MQ=255)
                                                                                                                              aTTGGGGAGGCTAATCATGCTGAGACACAACGGTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGAt                       <  2:2102304/150‑1 (MQ=255)
                                                                                                                               ttGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGAtt                      <  2:601244/150‑1 (MQ=255)
                                                                                                                                 ggggAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGa                    >  2:4326199/1‑150 (MQ=255)
                                                                                                                                  gggAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGaa                   <  1:1781204/150‑1 (MQ=255)
                                                                                                                                  gggAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGaa                   <  1:3435686/150‑1 (MQ=255)
                                                                                                                                    gAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAaga                 <  2:1340255/150‑1 (MQ=255)
                                                                                                                                    gAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAaga                 <  2:1405578/150‑1 (MQ=255)
                                                                                                                                    gAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAaga                 <  1:238413/150‑1 (MQ=255)
                                                                                                                                        cTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAg             >  1:3226252/1‑150 (MQ=255)
                                                                                                                                        cTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAg             <  2:349495/150‑1 (MQ=255)
                                                                                                                                        cTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAg             >  1:1032594/1‑150 (MQ=255)
                                                                                                                                         tAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCGGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTCTTGGGATGAGAGTCGGGCTTCCCGTTTTACTCTGCGCCAGATTGAAGAGCAGa            >  1:843959/1‑150 (MQ=255)
                                                                                                                                           aTCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATc          <  2:4297642/150‑1 (MQ=255)
                                                                                                                                              aTGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGaa       >  2:842305/1‑150 (MQ=255)
                                                                                                                                              aTGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGaa       >  2:3844450/1‑150 (MQ=255)
                                                                                                                                              aTGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGaa       >  2:2515671/1‑150 (MQ=255)
                                                                                                                                              aTGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAAGCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGaa       >  2:2203855/1‑150 (MQ=255)
                                                                                                                                                   gagaCACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAAcc  <  1:2976115/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAACC  >  CP009273/4402703‑4402999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑