Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,402,870 G→T 11.1% R9L (CGA→CTA)  yjfC → ATP‑Grasp family ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,402,8700GT11.1% 852.1 / 62.5 253R9L (CGA→CTA) yjfCATP‑Grasp family ATPase
Reads supporting (aligned to +/- strand):  ref base G (120/105);  new base T (16/12);  total (136/117)
Fisher's exact test for biased strand distribution p-value = 8.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 254 total aligned reads displayed.

GCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAACCGACTGCGGAACTGCAT  >  CP009273/4402721‑4403015
                                                                                                                                                     |                                                                                                                                                 
gcTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCg                                                                                                                                                   >  1:967161/1‑150 (MQ=255)
      gTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGa                                                                                                                                             >  1:277135/1‑150 (MQ=255)
       ttCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGAt                                                                                                                                            >  2:644177/1‑150 (MQ=255)
          tGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTg                                                                                                                                         <  1:2917935/150‑1 (MQ=255)
           ggCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCCTTGGGGAGGCTAATCATGCTGAGACAAAACGTTCCTGTGCTACGGGATCTgg                                                                                                                                        >  1:1523238/1‑150 (MQ=255)
             cAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGAc                                                                                                                                      <  1:1450093/150‑1 (MQ=255)
               aaaaaCAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCa                                                                                                                                    >  2:2495572/1‑150 (MQ=255)
               aaaaaCAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCa                                                                                                                                    >  2:3396117/1‑150 (MQ=255)
                   agagTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGATc                                                                                                                                >  2:3541196/1‑150 (MQ=255)
                   agagTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATc                                                                                                                                >  2:466087/1‑150 (MQ=255)
                   agagTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATc                                                                                                                                <  2:3938051/150‑1 (MQ=255)
                      gTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCCTGGGGCTTGGGGGGGCTAATAAAGCTGAGACACAACCTTACGGTGCGACGGGATCGGGACCCGATagcc                                                                                                                             >  1:737131/1‑150 (MQ=255)
                      gTCTGACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATcgcc                                                                                                                             <  1:40546/150‑1 (MQ=255)
                       tCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATcgccg                                                                                                                            <  1:3363088/150‑1 (MQ=255)
                        cTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTGTTCCAGCGCCCGTGGGCATTGGGGCGGCTAATCAAGCTGAGACACAACGTTCCTGTGCGACGGGATATGGACCAGATagccgc                                                                                                                           >  1:2579869/1‑150 (MQ=255)
                         ttACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACCACGTTCCTGTGCTACGGGATCTGGACCAGATcgccgcg                                                                                                                          >  2:1889311/1‑149 (MQ=255)
                         ttACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCt                                                                                                                          <  1:1241434/150‑1 (MQ=255)
                          tACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCATCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATAGCCGCTg                                                                                                                         >  1:2486658/1‑150 (MQ=255)
                          tACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCGTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTg                                                                                                                         >  1:235200/1‑150 (MQ=255)
                              ctTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACaa                                                                                                                     >  2:3027663/1‑150 (MQ=255)
                              ctTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACaa                                                                                                                     >  2:668065/1‑150 (MQ=255)
                                 cGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGATCGCCGCTGACAACgg                                                                                                                  <  2:1610579/150‑1 (MQ=255)
                                  gggCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGt                                                                                                                 <  1:3962774/150‑1 (MQ=255)
                                     cggcTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTc                                                                                                              <  1:3125151/150‑1 (MQ=255)
                                     cggcTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTc                                                                                                              <  1:3414296/150‑1 (MQ=255)
                                        cTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGAc                                                                                                           >  2:3973069/1‑150 (MQ=255)
                                         tttACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACt                                                                                                          >  1:2683442/1‑150 (MQ=255)
                                          ttACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACtt                                                                                                         >  2:4318933/1‑150 (MQ=255)
                                           tACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACACCGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACttt                                                                                                        <  1:3040502/150‑1 (MQ=255)
                                            accaccAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTc                                                                                                       <  1:2225781/150‑1 (MQ=255)
                                              caccaAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTACAGCGCCCCTGGGCCTTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCat                                                                                                     >  1:4062868/1‑150 (MQ=255)
                                                ccaAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCatat                                                                                                   <  1:2801158/150‑1 (MQ=255)
                                                ccaAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCatat                                                                                                   <  1:3469703/150‑1 (MQ=255)
                                                 caAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATatc                                                                                                  >  2:2251962/1‑150 (MQ=255)
                                                 caAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATatc                                                                                                  >  2:914880/1‑150 (MQ=255)
                                                  aaaaaaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGGGGGCCTTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATatca                                                                                                 >  2:2249731/1‑150 (MQ=255)
                                                  aaaaaaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTACAGCGCCCGTGGGGAGTGGGGAGGCTAATCATTCTGAGACACAACGTTCCTGTGAGACGGGATCTGGACCAGATAGCCGCTGACAACGGTTTAGACTTTCatataa                                                                                                 >  1:3037874/1‑148 (MQ=255)
                                                   aaaaaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATatcat                                                                                                <  2:1565245/150‑1 (MQ=255)
                                                    aaaaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATatcatc                                                                                               <  2:1277768/150‑1 (MQ=255)
                                                    aaaaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATatcatc                                                                                               >  2:1364723/1‑150 (MQ=255)
                                                     aaaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCg                                                                                              <  1:654040/150‑1 (MQ=255)
                                                          gTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACCGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAAt                                                                                         >  1:199944/1‑150 (MQ=255)
                                                           tCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATg                                                                                        >  1:2505023/1‑150 (MQ=255)
                                                           tCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATg                                                                                        <  1:1632913/150‑1 (MQ=255)
                                                             tACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa                                                                                      >  2:873487/1‑150 (MQ=255)
                                                               ccGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAAt                                                                                    <  1:3542311/150‑1 (MQ=255)
                                                                    tctcGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTAtt                                                                               <  1:1725671/150‑1 (MQ=255)
                                                                         cggcggCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTACTGTGCTACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTAATATCATCGACAATTAAATCTATTGGGAt                                                                          >  1:2733229/1‑150 (MQ=255)
                                                                          ggcggcTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATg                                                                         <  2:3762316/150‑1 (MQ=255)
                                                                          ggcggcTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATg                                                                         >  2:1371423/1‑150 (MQ=255)
                                                                          ggcggcTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCACTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATg                                                                         <  2:1926379/150‑1 (MQ=255)
                                                                           gcggcTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATga                                                                        <  1:56160/150‑1 (MQ=255)
                                                                             ggcTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATAATTCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATAGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATgaga                                                                      >  1:2745735/1‑150 (MQ=255)
                                                                               cTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGt                                                                    <  2:3381155/150‑1 (MQ=255)
                                                                               cTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGt                                                                    <  2:4152186/150‑1 (MQ=255)
                                                                               cTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGt                                                                    <  2:2920528/150‑1 (MQ=255)
                                                                                tATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTc                                                                   <  1:1433960/150‑1 (MQ=255)
                                                                                tATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTc                                                                   <  2:2007607/150‑1 (MQ=255)
                                                                                   ggTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCggg                                                                >  1:179844/1‑150 (MQ=255)
                                                                                     tCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCt                                                              <  1:1268399/150‑1 (MQ=255)
                                                                                     tCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCt                                                              >  2:723848/1‑150 (MQ=255)
                                                                                       gTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTa                                                            <  1:488233/150‑1 (MQ=255)
                                                                                        ttcttcCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTAc                                                           <  2:3079743/150‑1 (MQ=255)
                                                                                         tcttcCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTAcc                                                          >  2:253998/1‑150 (MQ=255)
                                                                                         tcttcCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTAcc                                                          >  2:2813937/1‑150 (MQ=255)
                                                                                         tcttcCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTAcc                                                          >  1:3841059/1‑150 (MQ=255)
                                                                                             ccAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGttt                                                      >  1:1342500/1‑150 (MQ=255)
                                                                                             ccAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGttt                                                      >  2:2873020/1‑150 (MQ=255)
                                                                                              cAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGtttt                                                     >  2:3940515/1‑150 (MQ=255)
                                                                                               aGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTa                                                    >  1:1769464/1‑150 (MQ=255)
                                                                                               aGCGCCCGTGGGCAGGGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTCTTGGGATGAGAGTCGGGCTTACCGTTTTa                                                    >  2:495825/1‑150 (MQ=255)
                                                                                                gcgcCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTAc                                                   <  2:1045769/150‑1 (MQ=255)
                                                                                                   cccGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTActct                                                >  1:3674308/1‑150 (MQ=255)
                                                                                                      gTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTgcg                                             >  2:3616135/1‑150 (MQ=255)
                                                                                                          gCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTAATCTGCGCCAg                                         >  2:3337269/1‑150 (MQ=255)
                                                                                                                 ggAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCATACCGTTTTACTCTGCGCCAGATTGAag                                  <  1:2126418/150‑1 (MQ=255)
                                                                                                                  gAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAaga                                 <  2:1340255/150‑1 (MQ=255)
                                                                                                                   aGGCTAATCATGCTTAGTCACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACATCGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAagag                                <  2:205818/150‑1 (MQ=255)
                                                                                                                   aGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAagag                                >  2:4287131/1‑150 (MQ=255)
                                                                                                                      cTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAg                             >  1:3225581/1‑150 (MQ=255)
                                                                                                                      cTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAg                             >  1:1032594/1‑150 (MQ=255)
                                                                                                                      cTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAg                             <  2:349495/150‑1 (MQ=255)
                                                                                                                      cTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAg                             >  2:3521100/1‑150 (MQ=255)
                                                                                                                       tAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGa                            <  2:3205191/150‑1 (MQ=255)
                                                                                                                       tAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCGGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTCTTGGGATGAGAGTCGGGCTTCCCGTTTTACTCTGCGCCAGATTGAAGAGCAGa                            >  1:843959/1‑150 (MQ=255)
                                                                                                                         aaaaTGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGTCTAACCGTTTTACTCTGCGCAAGATAGAAGATCAGaga                          >  2:3855448/4‑148 (MQ=255)
                                                                                                                         aTCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATc                          <  1:937715/150‑1 (MQ=255)
                                                                                                                            aTGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGaa                       >  2:3844450/1‑150 (MQ=255)
                                                                                                                            aTGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAAGCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGaa                       >  2:2203855/1‑150 (MQ=255)
                                                                                                                                 gagaCACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAAcc                  <  1:2976115/150‑1 (MQ=255)
                                                                                                                                     cacaACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAACCGACt              >  2:2841270/1‑150 (MQ=255)
                                                                                                                                      acaACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCTCTGACAACGTTTTCGAATTTCATATAATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGACAGATTGAAGAGCAGATAGAAAAACCGACTg             >  1:1704269/1‑150 (MQ=255)
                                                                                                                                        aaCGTTCCTGTGCGACGGTATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAACCGACTGCg           <  2:2421792/150‑1 (MQ=255)
                                                                                                                                        aaCGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAACCGACTGCg           >  1:2586182/1‑150 (MQ=255)
                                                                                                                                          cGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAACCGACTGCGGa         >  2:3316543/1‑150 (MQ=255)
                                                                                                                                           tttCGTGTGGGACGGTATTTGGACCATATGGCGGCTTACAAGGTTTTCGACTTTCATATCATCGACAATTAAATCTATTGGGTTGAGAGTCGGGCTTACATTTTTACTCTGCGCCAGTTTTATGAGCAGATCTAAAAACCGACTGCGGaa        <  2:2579869/149‑1 (MQ=255)
                                                                                                                                            ttCCGGTGCGCGGGTATCTGGACCAGATCGCCGCTGACAAGGGTTTCGACTTTCATATCACCGACAATTAAATCTTTTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAACCGACTGCGGAAc       <  1:4310532/150‑1 (MQ=255)
                                                                                                                                              ccTGTGCGACGGGATCTGGACAAGATCGCCGCTGACAACGTTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAACCGACTGCGGAACTg     <  2:2584323/150‑1 (MQ=255)
                                                                                                                                                tgtgCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAACCGACTGCGGAACTGCa   >  1:1044222/1‑150 (MQ=255)
                                                                                                                                                 gtgCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAACCGACTGCGGAACTGCAt  >  2:25230/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAACCGACTGCGGAACTGCAT  >  CP009273/4402721‑4403015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑