BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,462,966	A→T	26.7%	Y21N (TAT→AAT)	yjgH ←	UPF0131 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,462,966	0	A	T	26.7%	497.2 / 223.2	258	Y21N (TAT→AAT)	yjgH	UPF0131 family protein
Reads supporting (aligned to +/- strand): ref base A (88/101); new base T (36/33); total (124/134)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 260 total aligned reads displayed.

GTGCATCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACAAAACCCA  >  CP009273/4462818‑4463115

gTGCATCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATaa                                                                                                                                                      >  2:1955860/1‑150 (MQ=255)

   cATCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGa                                                                                                                                                   >  1:2237690/1‑150 (MQ=255)

    aTCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGAt                                                                                                                                                  <  1:1902775/150‑1 (MQ=255)

    aTCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGAt                                                                                                                                                  <  1:2521728/150‑1 (MQ=255)

     tCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATg                                                                                                                                                 >  2:69673/1‑150 (MQ=255)

       ccGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCt                                                                                                                                               <  2:2657385/150‑1 (MQ=255)

        cGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTc                                                                                                                                              >  1:943369/1‑150 (MQ=255)

           aGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGc                                                                                                                                           <  2:2293136/150‑1 (MQ=255)

              tGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCata                                                                                                                                        >  1:2448857/1‑150 (MQ=255)

               gCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCatct                                                                                                                                       >  2:3798203/1‑148 (MQ=255)

                ccAGGGTCGCATGGAAATTATCAAATGCCAGTATGACTTGTTGCTGTAAATCGGGTTATTGTGTTCCGTCCTCTCGACTTCCTACTTGCACGGAAACAAACAGCAAATCGCCGGAACGTATTGCCGCATAATTAATATGCTCAGCAtata                                                                                                                                      <  2:3667190/150‑1 (MQ=255)

                ccAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCAtata                                                                                                                                      <  2:1477470/150‑1 (MQ=255)

                  aGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGt                                                                                                                                    >  1:1282248/1‑150 (MQ=255)

                   gggTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTg                                                                                                                                   <  1:3309814/150‑1 (MQ=255)

                   gggTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTg                                                                                                                                   <  2:3430123/150‑1 (MQ=255)

                   gggTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTg                                                                                                                                   <  2:2418046/150‑1 (MQ=255)

                        gCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGt                                                                                                                              >  1:2970369/1‑150 (MQ=255)

                         cATGCATATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTc                                                                                                                             <  2:4152486/150‑1 (MQ=255)

                         cATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTc                                                                                                                             >  2:1090698/1‑150 (MQ=255)

                           tGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCgg                                                                                                                           <  2:4078657/150‑1 (MQ=255)

                                  tATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCcagcag                                                                                                                    >  1:2041123/1‑150 (MQ=255)

                                  tATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCcagcag                                                                                                                    >  1:4257623/1‑150 (MQ=255)

                                    tCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAggg                                                                                                                  >  2:2108181/1‑150 (MQ=255)

                                     cAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGa                                                                                                                 >  2:4267677/1‑150 (MQ=255)

                                           ccAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAg                                                                                                           <  1:3123777/150‑1 (MQ=255)

                                           ccAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAg                                                                                                           <  2:3399236/150‑1 (MQ=255)

                                             cgTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGACCGAATAGCCGCAGACTAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCg                                                                                                         >  2:1974095/2‑150 (MQ=255)

                                             aGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCg                                                                                                         >  1:4072408/1‑150 (MQ=255)

                                             aGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCg                                                                                                         >  1:1092702/1‑150 (MQ=255)

                                             aGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCg                                                                                                         >  2:514192/1‑150 (MQ=255)

                                             aGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCg                                                                                                         >  1:1543950/1‑150 (MQ=255)

                                                  gACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGttct                                                                                                    <  1:2887979/150‑1 (MQ=255)

                                                    cTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGttcttt                                                                                                  >  1:3847030/1‑150 (MQ=255)

                                                      tgttgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGttctttct                                                                                                >  2:3855004/1‑150 (MQ=255)

                                                      tgttgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGttctttct                                                                                                >  2:1668562/1‑150 (MQ=255)

                                                         tgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTAcc                                                                                             <  2:2576278/150‑1 (MQ=255)

                                                            tGAAAATCGGTTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGTATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCAtt                                                                                          <  1:3677286/150‑1 (MQ=255)

                                                            tGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCAtt                                                                                          >  1:810262/1‑150 (MQ=255)

                                                              aaaaTCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTg                                                                                        <  2:2874433/150‑1 (MQ=255)

                                                               aaaTCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTgg                                                                                       <  2:1074620/150‑1 (MQ=255)

                                                                aaTCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGt                                                                                      >  1:3486848/1‑150 (MQ=255)

                                                                aaTCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGt                                                                                      <  1:508548/150‑1 (MQ=255)

                                                                 aTCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGtt                                                                                     <  2:1939653/150‑1 (MQ=255)

                                                                 aTCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGtt                                                                                     <  2:4128505/150‑1 (MQ=255)

                                                                       ttCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTc                                                                               <  2:38867/150‑1 (MQ=255)

                                                                            gtgtTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGAt                                                                          <  2:224285/150‑1 (MQ=255)

                                                                             tgtTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGAtt                                                                         <  1:2458623/150‑1 (MQ=255)

                                                                              gtTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGAttt                                                                        <  1:4168830/150‑1 (MQ=255)

                                                                              gtTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGAttt                                                                        <  2:3193677/150‑1 (MQ=255)

                                                                               ttCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTa                                                                       <  2:4033914/150‑1 (MQ=255)

                                                                                 ccGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACg                                                                     >  1:1316519/1‑150 (MQ=255)

                                                                                  cGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGACTGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGa                                                                    >  1:609247/1‑150 (MQ=255)

                                                                                  cGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGa                                                                    >  1:1059999/1‑150 (MQ=255)

                                                                                  cGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGa                                                                    <  1:100163/150‑1 (MQ=255)

                                                                                    tCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACa                                                                  <  1:1982608/150‑1 (MQ=255)

                                                                                     ccTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACAt                                                                 <  2:3501971/150‑1 (MQ=255)

                                                                                     ccTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACAt                                                                 <  1:2391820/150‑1 (MQ=255)

                                                                                      ctctCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATg                                                                <  2:1861175/150‑1 (MQ=255)

                                                                                        ctcGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGaa                                                              >  2:1156135/1‑150 (MQ=255)

                                                                                         tcGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAAc                                                             <  2:3559793/150‑1 (MQ=255)

                                                                                          cGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACa                                                            >  1:1351735/1‑150 (MQ=255)

                                                                                           gACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACag                                                           <  1:1656694/150‑1 (MQ=255)

                                                                                             cTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACagaa                                                         >  2:1422118/1‑150 (MQ=255)

                                                                                             cTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACagaa                                                         >  2:2972713/1‑150 (MQ=255)

                                                                                              ttCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACagaag                                                        <  2:2977168/150‑1 (MQ=255)

                                                                                              ttCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACagaag                                                        >  1:3593558/1‑150 (MQ=255)

                                                                                                      tGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTctct                                                >  1:3876125/1‑150 (MQ=255)

                                                                                                         ccGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTa                                             <  1:1592330/150‑1 (MQ=255)

                                                                                                           ggAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTAcc                                           >  1:2906626/1‑150 (MQ=255)

                                                                                                           ggAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTAcc                                           >  1:1736060/1‑150 (MQ=255)

                                                                                                            gAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCg                                          <  2:3724860/150‑1 (MQ=255)

                                                                                                               acaaacaGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGa                                       <  2:4290597/150‑1 (MQ=255)

                                                                                                                caaacaGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAg                                      <  2:2513287/150‑1 (MQ=255)

                                                                                                                caaacaGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAg                                      <  2:2513275/150‑1 (MQ=255)

                                                                                                                caaacaGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAg                                      >  2:478986/1‑150 (MQ=255)

                                                                                                                 aaacaGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGc                                     >  1:2339831/1‑150 (MQ=255)

                                                                                                                  aacaGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGcc                                    >  2:287610/1‑150 (MQ=255)

                                                                                                                  aacaGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGcc                                    <  1:25323/150‑1 (MQ=255)

                                                                                                                  aacaGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGcc                                    >  2:1489006/1‑150 (MQ=255)

                                                                                                                  aacaGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGcc                                    <  1:2814211/150‑1 (MQ=255)

                                                                                                                      gCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCtt                                <  2:2754860/150‑1 (MQ=255)

                                                                                                                        aaaTCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCtttt                              >  2:3947687/1‑150 (MQ=255)

                                                                                                                        aaaTCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCtttt                              >  1:2341610/1‑150 (MQ=255)

                                                                                                                        aaaTCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCtttt                              <  1:734061/150‑1 (MQ=255)

                                                                                                                         aaTCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTa                             <  1:111176/150‑1 (MQ=255)

                                                                                                                          aTCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAg                            <  2:2041123/150‑1 (MQ=255)

                                                                                                                           tCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGc                           >  1:3697979/1‑150 (MQ=255)

                                                                                                                             gCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCgg                         >  1:1426107/1‑150 (MQ=255)

                                                                                                                               cGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGgac                       >  2:3156808/1‑150 (MQ=255)

                                                                                                                                      aaTAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGa                >  2:4186901/1‑150 (MQ=255)

                                                                                                                                       aTAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAg               <  2:1260501/150‑1 (MQ=255)

                                                                                                                                       aTAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAg               >  1:1157416/1‑150 (MQ=255)

                                                                                                                                        tAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGt              >  2:3325528/1‑150 (MQ=255)

                                                                                                                                        tAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGt              <  1:3519252/150‑1 (MQ=255)

                                                                                                                                         aGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTa             <  2:4072408/150‑1 (MQ=255)

                                                                                                                                           ccGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTaaa           <  2:1595454/150‑1 (MQ=255)

                                                                                                                                            cGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAAc          >  2:1377534/1‑150 (MQ=255)

                                                                                                                                            cGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAAc          >  1:3816416/1‑150 (MQ=255)

                                                                                                                                               aGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACaaa       >  2:625972/1‑150 (MQ=255)

                                                                                                                                                    aaCGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACAAAACCCa  >  2:4100023/1‑150 (MQ=255)

GTGCATCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACAAAACCCA  >  CP009273/4462818‑4463115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`