BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,470,873	G→T	48.5%	R928S (CGT→AGT)	valS ←	valyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,470,873	0	G	T	48.5%	116.5 / inf	286	R928S (CGT→AGT)	valS	valyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (86/61); new base T (72/67); total (158/128)
Fisher's exact test for biased strand distribution p-value = 2.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

TCTTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGGCCAGCCATCG  >  CP009273/4470726‑4471022

    tACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCttt                                                                                                                                                 <  2:4135019/150‑1 (MQ=255)

      ccATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCCGCTTCTCACGCTCTTTcg                                                                                                                                               >  1:2033834/1‑150 (MQ=255)

       cATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTcgc                                                                                                                                              >  1:699226/1‑150 (MQ=255)

        aTTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTcgcg                                                                                                                                             >  2:2014728/1‑150 (MQ=255)

          ttCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGAt                                                                                                                                           <  1:3951572/150‑1 (MQ=255)

          ttCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGAt                                                                                                                                           >  2:2861918/1‑150 (MQ=255)

             tGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGAc                                                                                                                                        <  2:1276515/150‑1 (MQ=255)

               tAAGAGATAAAAATGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCg                                                                                                                                      >  2:2460235/1‑150 (MQ=255)

               tAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCg                                                                                                                                      >  2:3741905/1‑150 (MQ=255)

                 agagATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCt                                                                                                                                    >  2:5807/1‑150 (MQ=255)

                 agagATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTAATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATTACCgat                                                                                                                                    >  1:3115812/1‑148 (MQ=255)

                      tAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCgg                                                                                                                               <  2:1815057/150‑1 (MQ=255)

                      tAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCGTCCgg                                                                                                                               >  2:2171519/1‑150 (MQ=255)

                       aaaaaaGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGt                                                                                                                              <  2:132880/150‑1 (MQ=255)

                       aaaaaaGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGt                                                                                                                              >  2:486125/1‑150 (MQ=255)

                          aaaGGCCGGAGCATGCTCCGGCCTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGATCGCTTCCGGTgcg                                                                                                                           >  2:101277/1‑150 (MQ=255)

                           aaGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTgcgc                                                                                                                          <  2:721073/150‑1 (MQ=255)

                            aGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGcgcg                                                                                                                         >  1:2146053/1‑150 (MQ=255)

                                 ggAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGa                                                                                                                    <  2:1509330/150‑1 (MQ=255)

                                     cATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACaaa                                                                                                                <  1:3743598/150‑1 (MQ=255)

                                      aTGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAg                                                                                                               >  2:3347078/1‑150 (MQ=255)

                                         cTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCt                                                                                                            >  2:3679271/1‑150 (MQ=255)

                                          tCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCtt                                                                                                           >  1:1423445/1‑150 (MQ=255)

                                           ccGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttc                                                                                                          <  1:1215139/150‑1 (MQ=255)

                                           ccGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttc                                                                                                          >  2:2318720/1‑150 (MQ=255)

                                              gCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttcgtt                                                                                                       <  1:2662887/150‑1 (MQ=255)

                                               ccTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttcgttc                                                                                                      >  2:1656956/1‑150 (MQ=255)

                                                cTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATATCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCATCCGGTGCGCGGGCGACAAAGCCttcgttcg                                                                                                     >  1:3334969/1‑150 (MQ=255)

                                                 ttCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGc                                                                                                    >  2:1923472/1‑150 (MQ=255)

                                                   cGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCa                                                                                                  <  1:611393/150‑1 (MQ=255)

                                                     ttttCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGt                                                                                                >  1:1616804/1‑150 (MQ=255)

                                                       ttCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGttt                                                                                              <  2:2364838/150‑1 (MQ=255)

                                                          atcaCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGtt                                                                                           <  1:1444227/150‑1 (MQ=255)

                                                            caCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTtct                                                                                         <  2:3893184/150‑1 (MQ=255)

                                                               tgtgTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGa                                                                                      >  1:210449/1‑150 (MQ=255)

                                                                     ttGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATCCGGc                                                                                <  2:3713994/150‑1 (MQ=255)

                                                                     ttGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGc                                                                                <  2:3418991/150‑1 (MQ=255)

                                                                      tGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCt                                                                               >  1:1004904/1‑150 (MQ=255)

                                                                       gATTACAGCGCGGCGATAACAGCCTGATGATCAATCAGATACGCTTTCGATTCCGCATATCCTACCAGCGTCGCACTATCTTTCGCCAATACCTATTCCGCTGCGCGGGCGACAAAGCATACGTGCGACACTTTTTTATCGATACGGCTg                                                                              >  1:2727517/1‑150 (MQ=255)

                                                                        aTTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGa                                                                             <  1:3404324/150‑1 (MQ=255)

                                                                        aTTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGa                                                                             >  1:1330592/1‑150 (MQ=255)

                                                                         ttACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGAGGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGAt                                                                            <  2:2940617/150‑1 (MQ=255)

                                                                         ttACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGAt                                                                            >  1:689480/1‑150 (MQ=255)

                                                                          tACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGAtt                                                                           <  1:3679271/150‑1 (MQ=255)

                                                                          tACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGAtt                                                                           <  1:73191/150‑1 (MQ=255)

                                                                          tACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGAtt                                                                           <  1:3519495/150‑1 (MQ=255)

                                                                                gcggcgATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCt                                                                     >  1:2550144/1‑150 (MQ=255)

                                                                                gcggcgATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCt                                                                     <  2:3045784/150‑1 (MQ=255)

                                                                                gcggcgATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCt                                                                     <  1:2984036/150‑1 (MQ=255)

                                                                                 cggcgATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCtt                                                                    <  1:284111/150‑1 (MQ=255)

                                                                                  ggcgATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTc                                                                   >  2:1268482/1‑150 (MQ=255)

                                                                                  ggcgATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTc                                                                   >  2:3462356/1‑150 (MQ=255)

                                                                                    cgATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCaa                                                                 >  1:4247635/1‑150 (MQ=255)

                                                                                     gATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAAt                                                                >  2:1183044/1‑150 (MQ=255)

                                                                                     gATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAAt                                                                <  2:3515530/150‑1 (MQ=255)

                                                                                      aTAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATc                                                               >  2:4077422/1‑150 (MQ=255)

                                                                                        aaCAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCATCAATCtt                                                             >  2:2479851/1‑150 (MQ=255)

                                                                                          cAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCg                                                           <  1:865122/150‑1 (MQ=255)

                                                                                          cAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCg                                                           <  1:188900/150‑1 (MQ=255)

                                                                                          cAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCg                                                           <  1:3043411/150‑1 (MQ=255)

                                                                                           aGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGc                                                          >  2:3981400/1‑150 (MQ=255)

                                                                                           aGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGc                                                          >  1:655458/1‑150 (MQ=255)

                                                                                             ccTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCa                                                        >  1:2432848/1‑150 (MQ=255)

                                                                                              ctgctgTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCAc                                                       >  2:2547506/1‑150 (MQ=255)

                                                                                                gctgTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCActt                                                     <  2:40713/150‑1 (MQ=255)

                                                                                                  tgTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCGTCGCCActtct                                                   >  1:2251455/1‑150 (MQ=255)

                                                                                                    ttCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCttt                                                 <  2:3542799/150‑1 (MQ=255)

                                                                                                     tCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTc                                                <  1:152567/150‑1 (MQ=255)

                                                                                                     tCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTc                                                <  1:2938783/150‑1 (MQ=255)

                                                                                                       aaTCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTAACGCTCTTTAGCGATGACCGCTTCCGGTGCGCGGGCGACAAATCCTTAGTTCGCCAGTTTGTTCTCGATACGGCTTATTTCACCATCAATCTTCGCCACTTCTTTAGc                                              >  1:4043213/1‑150 (MQ=255)

                                                                                                        aTCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGcc                                             >  2:1522323/1‑150 (MQ=255)

                                                                                                         tCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCa                                            <  2:2256873/150‑1 (MQ=255)

                                                                                                            gTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGAc                                         <  2:1773948/150‑1 (MQ=255)

                                                                                                               tcgctttcgcttCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGAcgcg                                      <  1:2789978/150‑1 (MQ=255)

                                                                                                               tcgctttcgcttCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGAcgcg                                      <  2:2728727/150‑1 (MQ=255)

                                                                                                                 gctttcgcttCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGcc                                    >  2:513533/1‑150 (MQ=255)

                                                                                                                 gctttcgcttCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGcc                                    <  2:3803761/150‑1 (MQ=255)

                                                                                                                 gctttcgcttCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGACAGTTTGTTCTCGATACGGATGATTTCAACTTCAATCTTCGCCACTTCTTTCGCCAGACTCGcc                                    <  2:4703/150‑1 (MQ=255)

                                                                                                                  ctttcgcttCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCa                                   >  1:1096902/1‑150 (MQ=255)

                                                                                                                  ctttcgcttCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCa                                   >  1:1564782/1‑150 (MQ=255)

                                                                                                                     tcgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCt                                <  1:4318705/150‑1 (MQ=255)

                                                                                                                     tcgcttCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCt                                <  1:3303408/150‑1 (MQ=255)

                                                                                                                      cgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTc                               <  2:3116688/150‑1 (MQ=255)

                                                                                                                      cgcttCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTc                               >  1:1839285/1‑150 (MQ=255)

                                                                                                                       gcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTCCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCa                              <  1:4156076/150‑1 (MQ=255)

                                                                                                                       gcttCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCa                              >  2:838844/1‑150 (MQ=255)

                                                                                                                       gcttCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCa                              >  2:1878067/1‑150 (MQ=255)

                                                                                                                        cttCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAt                             <  2:1850413/150‑1 (MQ=255)

                                                                                                                        cttCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAt                             >  1:2991639/1‑150 (MQ=255)

                                                                                                                            cGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtcttc                         >  2:2537389/1‑150 (MQ=255)

                                                                                                                               aTAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCttt                      >  1:3904243/1‑150 (MQ=255)

                                                                                                                                 aGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTttgt                    >  1:2893300/1‑150 (MQ=255)

                                                                                                                                      tCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTtgatg               <  1:1273160/150‑1 (MQ=255)

                                                                                                                                        cAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAg             <  1:2459539/150‑1 (MQ=255)

                                                                                                                                           cTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGgcc          >  1:3438418/1‑150 (MQ=255)

                                                                                                                                            ttCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGgcca         <  2:2698329/150‑1 (MQ=255)

                                                                                                                                             tctcACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGgccag        <  1:472917/150‑1 (MQ=255)

                                                                                                                                             tctcACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGgccag        >  2:41666/1‑150 (MQ=255)

                                                                                                                                                 aCGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTGGTTGATGAGGCCAGCCAt    >  1:1695176/1‑150 (MQ=255)

                                                                                                                                                   gCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGGCCAGCCATCg  >  1:3629591/1‑150 (MQ=255)

TCTTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGGCCAGCCATCG  >  CP009273/4470726‑4471022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`