BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,494,721	G→A	45.5%	A283T (GCC→ACC)	yjhB →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,494,721	0	G	A	45.5%	72.1 / 259.3	180	A283T (GCC→ACC)	yjhB	putative transporter
Reads supporting (aligned to +/- strand): ref base G (42/56); new base A (41/41); total (83/97)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

Only 100 of 181 total aligned reads displayed.

CGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTATATAT  >  CP009273/4494575‑4494870

cGATACACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCt                                                                                                                                                    <  2:2165885/150‑1 (MQ=255)

  aTAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCttt                                                                                                                                                  >  2:1970503/1‑150 (MQ=255)

    aaaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGt                                                                                                                                                <  2:2408251/150‑1 (MQ=255)

     aaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTa                                                                                                                                               >  1:1576812/1‑150 (MQ=255)

        ggACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTc                                                                                                                                            <  1:1843432/150‑1 (MQ=255)

        ggACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTc                                                                                                                                            <  2:3641252/150‑1 (MQ=255)

         gACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCg                                                                                                                                           >  2:2726438/1‑150 (MQ=255)

           ctactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGt                                                                                                                                         >  1:2814294/1‑150 (MQ=255)

            tactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTc                                                                                                                                        <  2:3856103/150‑1 (MQ=255)

             aattccttccTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCt                                                                                                                                       >  2:2115596/3‑150 (MQ=255)

                tccttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCtaat                                                                                                                                    <  1:1126817/150‑1 (MQ=255)

                   ttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAAc                                                                                                                                 <  1:1582892/150‑1 (MQ=255)

                   ttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAAc                                                                                                                                 <  1:2753066/150‑1 (MQ=255)

                   ttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAAc                                                                                                                                 <  2:1778908/150‑1 (MQ=255)

                    tccgACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACt                                                                                                                                <  2:1624536/150‑1 (MQ=255)

                       tACCTGGCATATAATGGATTTAATACAGTTGTAATTTCAACTATTATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCa                                                                                                                             <  2:15037/150‑1 (MQ=255)

                        aCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCat                                                                                                                            <  2:2494400/150‑1 (MQ=255)

                        aCCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATA‑TTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCatt                                                                                                                           >  1:3184381/1‑150 (MQ=255)

                         ccTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCatt                                                                                                                           >  1:606631/1‑150 (MQ=255)

                           tGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCattta                                                                                                                         >  2:3337670/1‑150 (MQ=255)

                            ggCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCatttat                                                                                                                        >  1:3372881/1‑150 (MQ=255)

                               aGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTAtttt                                                                                                                     >  1:1908056/1‑150 (MQ=255)

                               aGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTAtttt                                                                                                                     <  1:3373264/150‑1 (MQ=255)

                               aGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTAtttt                                                                                                                     >  1:2408905/1‑150 (MQ=255)

                               aGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACGTCATTTAtttt                                                                                                                     >  1:2410137/1‑150 (MQ=255)

                                 aTAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTcc                                                                                                                   <  1:1340410/150‑1 (MQ=255)

                                   aaTGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCtt                                                                                                                 <  2:2857730/150‑1 (MQ=255)

                                    aTGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCttt                                                                                                                >  2:3200178/1‑150 (MQ=255)

                                      ggAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCttttt                                                                                                              >  2:3447622/1‑148 (MQ=255)

                                        aGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTcc                                                                                                            <  2:277449/150‑1 (MQ=255)

                                        aGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTcc                                                                                                            >  1:265300/1‑150 (MQ=255)

                                         gTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCct                                                                                                           >  2:1833734/1‑150 (MQ=255)

                                             aTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCttt                                                                                                       <  1:3921828/150‑1 (MQ=255)

                                              tACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtttt                                                                                                      <  2:2308936/150‑1 (MQ=255)

                                              tACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtttt                                                                                                      >  1:1077635/1‑150 (MQ=255)

                                              tACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCAGTTATTTTGGTTTGTCGTCtttt                                                                                                      >  1:1077552/1‑150 (MQ=255)

                                               aCAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCttttt                                                                                                     <  2:4023864/150‑1 (MQ=255)

                                                   tGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTa                                                                                                 >  1:3598940/1‑150 (MQ=255)

                                                    ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTAt                                                                                                <  2:2908326/150‑1 (MQ=255)

                                                    ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTAt                                                                                                <  2:736802/150‑1 (MQ=255)

                                                      tCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTAttt                                                                                              <  1:850338/150‑1 (MQ=255)

                                                      tCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTAttt                                                                                              <  1:2689377/150‑1 (MQ=255)

                                                        aTTTCAACTCTGATGACAATAGCAGTTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCt                                                                                            <  2:3176853/150‑1 (MQ=255)

                                                        aTTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCt                                                                                            <  1:3863620/150‑1 (MQ=255)

                                                         tttCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCtg                                                                                           <  1:4261828/150‑1 (MQ=255)

                                                         tttCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCtg                                                                                           >  2:3459425/1‑150 (MQ=255)

                                                          ttCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCtg                                                                                           >  2:4059331/1‑150 (MQ=255)

                                                             aaCTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGaa                                                                                       >  1:66873/1‑150 (MQ=255)

                                                                      gaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttc                                                                              <  1:3534150/150‑1 (MQ=255)

                                                                       aCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttct                                                                             <  1:4199707/150‑1 (MQ=255)

                                                                        cAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctc                                                                            >  1:341859/1‑150 (MQ=255)

                                                                        cAATAGCAGGGTTAGGAACACTGACAGGTACAATATTGTTGGGTATTGTGGGAGATAAGATTGGTGT‑AAAAAAAGCCGTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctc                                                                            <  2:3487423/150‑1 (MQ=255)

                                                                         aaTAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttatct                                                                           >  2:3594974/1‑150 (MQ=255)

                                                                         aaTAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctct                                                                           <  1:2616522/150‑1 (MQ=255)

                                                                         aaTAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctct                                                                           <  1:442247/150‑1 (MQ=255)

                                                                          aTAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctctt                                                                          <  2:1838592/150‑1 (MQ=255)

                                                                             gCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTtata                                                                       >  1:2570350/1‑150 (MQ=255)

                                                                              cAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAg                                                                      >  2:1793793/1‑150 (MQ=255)

                                                                               aGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAgg                                                                     >  2:183954/1‑150 (MQ=255)

                                                                                ggTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGa                                                                    <  1:4160277/150‑1 (MQ=255)

                                                                                ggTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGa                                                                    <  2:3577225/150‑1 (MQ=255)

                                                                                    tAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTAt                                                                <  2:4241241/150‑1 (MQ=255)

                                                                                     aGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAACAACTCTTCTCTTATAGGATTATg                                                               >  2:2591236/1‑150 (MQ=255)

                                                                                     aGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATg                                                               >  2:2591122/1‑150 (MQ=255)

                                                                                        aaCACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGCTTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtct                                                            <  1:611020/150‑1 (MQ=255)

                                                                                        aaCACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtct                                                            <  1:611019/150‑1 (MQ=255)

                                                                                        aaCACTGACAGGTACAATATTTTTTGGTTGTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtct                                                            <  2:2925225/150‑1 (MQ=255)

                                                                                              gACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTgg                                                      <  2:1137380/150‑1 (MQ=255)

                                                                                              gACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTgg                                                      >  1:2463614/1‑150 (MQ=255)

                                                                                                 aGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGAtt                                                   >  1:4326563/1‑150 (MQ=255)

                                                                                                  ggTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTATTATAGGATTATGTCTCTTTGGATTa                                                  >  2:1002681/1‑150 (MQ=255)

                                                                                                    tACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTATTCTCTTATAGGATTATGTCTCTTTGGATTAAt                                                >  2:1045280/1‑150 (MQ=255)

                                                                                                    tACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTATTCTCTTATAGGATTATGTCTCTTTGGATTAAt                                                >  2:1045289/1‑150 (MQ=255)

                                                                                                    tACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTATTCTCTTATAGGATTATGTCTCTTTGGATTAAt                                                >  2:1853288/1‑150 (MQ=255)

                                                                                                        atatTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTACTTTGTCCTCTTTTTTTTATTTCTGTGAAAAAATCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGttt                                            >  2:513989/1‑150 (MQ=255)

                                                                                                        atatTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGttt                                            <  1:2225007/150‑1 (MQ=255)

                                                                                                        atatTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGttt                                            <  1:4082013/150‑1 (MQ=255)

                                                                                                          atTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTAc                                          <  2:599732/150‑1 (MQ=255)

                                                                                                           ttttttGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACCTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACa                                         <  2:3663569/150‑1 (MQ=255)

                                                                                                                 tgTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTa                                   <  2:673568/149‑1 (MQ=255)

                                                                                                                    tttGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGt                                <  1:28988/150‑1 (MQ=255)

                                                                                                                       gttgGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTAtt                             <  2:4344814/150‑1 (MQ=255)

                                                                                                                         tgGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGc                           >  1:2302855/1‑150 (MQ=255)

                                                                                                                          ggTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCa                          <  2:568222/150‑1 (MQ=255)

                                                                                                                          ggTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCa                          <  2:2422070/150‑1 (MQ=255)

                                                                                                                           gTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAg                         >  2:3904115/1‑150 (MQ=255)

                                                                                                                           gTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAg                         >  2:3741482/1‑150 (MQ=255)

                                                                                                                               tAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggtt                     <  1:201246/150‑1 (MQ=255)

                                                                                                                               tAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATACCTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggtt                     >  2:2267078/1‑150 (MQ=255)

                                                                                                                                aaGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttg                    >  1:4063837/1‑150 (MQ=255)

                                                                                                                                 aGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttgg                   <  2:3372881/150‑1 (MQ=255)

                                                                                                                                  gATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttggt                  <  1:1090789/150‑1 (MQ=255)

                                                                                                                                   aTTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttggtt                 <  2:1237482/150‑1 (MQ=255)

                                                                                                                                   aTTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttggtt                 <  1:544353/150‑1 (MQ=255)

                                                                                                                                   aTTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttggtt                 <  1:2355946/150‑1 (MQ=255)

                                                                                                                                      ggTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCa              <  1:2153593/150‑1 (MQ=255)

                                                                                                                                      ggTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCa              <  1:2153181/150‑1 (MQ=255)

                                                                                                                                                aaaGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTtata     >  2:3551216/1‑150 (MQ=255)

                                                                                                                                                aaaGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTtata     <  2:925958/150‑1 (MQ=255)

                                                                                                                                                   gCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTatatat  >  2:16257/1‑150 (MQ=255)

CGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTATATAT  >  CP009273/4494575‑4494870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`